Imidazole--a new ligand for metal affinity precipitation: precipitation of Kunitz soybean trypsin inhibitor using Cu(II)-loaded copolymers of 1-vinylimidazole with N-vinylcaprolactam or N-isopropylacrylamide
Kunitz soybean trypsin inhibitor (STI) was specifically coprecipitated during precipitation of Cu(II)-loaded copolymers induced by increase in temperature and ionic strength. The copolymers used consisted of 1-vinylimidazole and N-vinylcaprolactam or N-isopropylacrylamide. The elution of STI was ach...
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| Veröffentlicht in: | Applied biochemistry and biotechnology 1997, Vol.68 (1/2), p.121-133 |
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| container_title | Applied biochemistry and biotechnology |
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| creator | Galaev, I.Y Kumar, A Agarwal, R Gupta, M.N Mattiasson, B |
| description | Kunitz soybean trypsin inhibitor (STI) was specifically coprecipitated during precipitation of Cu(II)-loaded copolymers induced by increase in temperature and ionic strength. The copolymers used consisted of 1-vinylimidazole and N-vinylcaprolactam or N-isopropylacrylamide. The elution of STI was achieved by solubilization of the STI-Cu(II)-polymer complex in the presence of an excess of the competing ligand, imidazole, and a subsequent precipitation of the polymer with STI remaining free in solution in a purified form as judged by Sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE). To the best of our knowledge this is the first reported successful metal affinity precipitation of protein in a heterobifunctional format. |
| doi_str_mv | 10.1007/BF02785985 |
| format | Article |
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| ispartof | Applied biochemistry and biotechnology, 1997, Vol.68 (1/2), p.121-133 |
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| source | SpringerNature Journals |
| subjects | Analytical biochemistry: general aspects, technics, instrumentation Analytical, structural and metabolic biochemistry Biological and medical sciences Biological Sciences Biotechnology Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Others Various methods and equipments |
| title | Imidazole--a new ligand for metal affinity precipitation: precipitation of Kunitz soybean trypsin inhibitor using Cu(II)-loaded copolymers of 1-vinylimidazole with N-vinylcaprolactam or N-isopropylacrylamide |
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