Imidazole--a new ligand for metal affinity precipitation: precipitation of Kunitz soybean trypsin inhibitor using Cu(II)-loaded copolymers of 1-vinylimidazole with N-vinylcaprolactam or N-isopropylacrylamide

Kunitz soybean trypsin inhibitor (STI) was specifically coprecipitated during precipitation of Cu(II)-loaded copolymers induced by increase in temperature and ionic strength. The copolymers used consisted of 1-vinylimidazole and N-vinylcaprolactam or N-isopropylacrylamide. The elution of STI was ach...

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Veröffentlicht in:Applied biochemistry and biotechnology 1997, Vol.68 (1/2), p.121-133
Hauptverfasser: Galaev, I.Y, Kumar, A, Agarwal, R, Gupta, M.N, Mattiasson, B
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container_issue 1/2
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container_title Applied biochemistry and biotechnology
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creator Galaev, I.Y
Kumar, A
Agarwal, R
Gupta, M.N
Mattiasson, B
description Kunitz soybean trypsin inhibitor (STI) was specifically coprecipitated during precipitation of Cu(II)-loaded copolymers induced by increase in temperature and ionic strength. The copolymers used consisted of 1-vinylimidazole and N-vinylcaprolactam or N-isopropylacrylamide. The elution of STI was achieved by solubilization of the STI-Cu(II)-polymer complex in the presence of an excess of the competing ligand, imidazole, and a subsequent precipitation of the polymer with STI remaining free in solution in a purified form as judged by Sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE). To the best of our knowledge this is the first reported successful metal affinity precipitation of protein in a heterobifunctional format.
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source SpringerNature Journals
subjects Analytical biochemistry: general aspects, technics, instrumentation
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Biological Sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Others
Various methods and equipments
title Imidazole--a new ligand for metal affinity precipitation: precipitation of Kunitz soybean trypsin inhibitor using Cu(II)-loaded copolymers of 1-vinylimidazole with N-vinylcaprolactam or N-isopropylacrylamide
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