Cloning and mutation of the gene encoding endothiapepsin from Cryphonectria parasitica

Endothiapepsin is an aspartic protease secreted by Cryphonectria parasitica. It has a milk-clotting activity and is used in the cheese industry. The eapA gene encoding endothiapepsin has been cloned and sequenced. An open reading frame of 419 codons, which encodes a precursor differing from mature e...

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Veröffentlicht in:	Current genetics 1992-05, Vol.21 (6), p.455-461
Hauptverfasser:	Razanamparany, V. (Sanofi Elf Biorecherches, Labege (France). Unite de Microbiologie), Jara, P, Legoux, R, Delmas, P, Msayeh, F, Kaghad, M, Loison, G
Format:	Artikel
Sprache:	eng
Schlagworte:	ACIDE ASPARTIQUE ACIDO ASPARTICO Amino Acid Sequence Ascomycota - genetics ASPARTIC ACID Aspartic Acid Endopeptidases - chemistry Aspartic Acid Endopeptidases - genetics Aspartyl protease Base Sequence Biological and medical sciences Blotting, Southern CLONACION MOLECULAR CLONAGE MOLECULAIRE Cloning, Molecular CRYPHONECTRIA PARASITICA eapA gene Endothiapepsin Fundamental and applied biological sciences. Psychology Fungal Proteins - chemistry Fungal Proteins - genetics GENE GENES Genes. Genome Molecular and cellular biology MOLECULAR CLONING Molecular genetics Molecular Sequence Data MUTANT MUTANTES MUTANTS Mutation - genetics nucleotide sequence Oligonucleotide Probes PEPSIN PEPSINA PEPSINE prediction PROTEASAS PROTEASE Protease-deficient mutant PROTEASES Restriction Mapping Transformation, Genetic - genetics
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