An improved suicide vector for construction of chromosomal insertion mutations in bacteria
We have constructed an R6K-based suicide vector (pJP5603) that requires a trans supply of the pir-encoded π protein of plasmid R6K for replication. Therefore, efficient plasmid suicide results upon transfer to bacteria not harbouring pir. The 3.1-kb vector encodes kanamycin resistance and is mobiliz...
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| Veröffentlicht in: | Gene 1992-09, Vol.118 (1), p.145-146 |
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| container_title | Gene |
| container_volume | 118 |
| creator | Penfold, Robert J. Pemberton, John M. |
| description | We have constructed an R6K-based suicide vector (pJP5603) that requires a
trans supply of the
pir-encoded π protein of plasmid R6K for replication. Therefore, efficient plasmid suicide results upon transfer to bacteria not harbouring
pir. The 3.1-kb vector encodes kanamycin resistance and is mobilizable. When used in conjunction with a JM109 strain carrying
pir, it has nine unique restriction sites available for α-complementation cloning. Vector functionality was demonstrated in
Rhodobacter sphaeroides. |
| doi_str_mv | 10.1016/0378-1119(92)90263-O |
| format | Article |
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trans supply of the
pir-encoded π protein of plasmid R6K for replication. Therefore, efficient plasmid suicide results upon transfer to bacteria not harbouring
pir. The 3.1-kb vector encodes kanamycin resistance and is mobilizable. When used in conjunction with a JM109 strain carrying
pir, it has nine unique restriction sites available for α-complementation cloning. Vector functionality was demonstrated in
Rhodobacter sphaeroides.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(92)90263-O</identifier><identifier>PMID: 1511879</identifier><identifier>CODEN: GENED6</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Bacterial Proteins - genetics ; Biological and medical sciences ; Biotechnology ; Chromosomes, Bacterial ; DNA Helicases ; DNA, Recombinant - genetics ; DNA-Binding Proteins ; Fundamental and applied biological sciences. Psychology ; Genetic engineering ; Genetic technics ; Genetic Vectors - genetics ; Methods. Procedures. Technologies ; mobilization ; Mutagenesis, Insertional - methods ; pir gene ; R6K replicon ; Recombinant DNA ; Replicon - genetics ; Rhodobacter sphaeroides ; Rhodobacter sphaeroides - genetics ; site-directed mutagenesis ; Trans-Activators ; Vectors (cloning, transfer, expression). Insertion sequences and transposons</subject><ispartof>Gene, 1992-09, Vol.118 (1), p.145-146</ispartof><rights>1992</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c534t-446636751247324675d559f12a37732237cea35cd42b6a5a559d27bc96d9babd3</citedby><cites>FETCH-LOGICAL-c534t-446636751247324675d559f12a37732237cea35cd42b6a5a559d27bc96d9babd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1119(92)90263-O$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5463355$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1511879$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Penfold, Robert J.</creatorcontrib><creatorcontrib>Pemberton, John M.</creatorcontrib><title>An improved suicide vector for construction of chromosomal insertion mutations in bacteria</title><title>Gene</title><addtitle>Gene</addtitle><description>We have constructed an R6K-based suicide vector (pJP5603) that requires a
trans supply of the
pir-encoded π protein of plasmid R6K for replication. Therefore, efficient plasmid suicide results upon transfer to bacteria not harbouring
pir. The 3.1-kb vector encodes kanamycin resistance and is mobilizable. When used in conjunction with a JM109 strain carrying
pir, it has nine unique restriction sites available for α-complementation cloning. Vector functionality was demonstrated in
Rhodobacter sphaeroides.</description><subject>Bacterial Proteins - genetics</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Chromosomes, Bacterial</subject><subject>DNA Helicases</subject><subject>DNA, Recombinant - genetics</subject><subject>DNA-Binding Proteins</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Genetic Vectors - genetics</subject><subject>Methods. Procedures. Technologies</subject><subject>mobilization</subject><subject>Mutagenesis, Insertional - methods</subject><subject>pir gene</subject><subject>R6K replicon</subject><subject>Recombinant DNA</subject><subject>Replicon - genetics</subject><subject>Rhodobacter sphaeroides</subject><subject>Rhodobacter sphaeroides - genetics</subject><subject>site-directed mutagenesis</subject><subject>Trans-Activators</subject><subject>Vectors (cloning, transfer, expression). Insertion sequences and transposons</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LwzAYx4Moc06_gUIPInqo5r3LRRjDNxjsohcvIU1SjLSNJu3Ab2-6jnkzEPLw_P_PS34AnCN4iyDid5AU8xwhJK4FvhEQc5KvD8AUzQuRQ0jmh2C6txyDkxg_YTqM4QmYIIYG3xS8L9rMNV_Bb6zJYu-0MzbbWN35kFXpat_GLvS6c77NfJXpj-AbH32j6sy10Yat0PSdGoKYclmpdGeDU6fgqFJ1tGe7dwbeHh9el8_5av30slyscs0I7XJKOSe8YAjTgmCaIsOYqBBWpEgJTAptFWHaUFxyxVQSDS5KLbgRpSoNmYGrsW_6xXdvYycbF7Wta9Va30eJOIGIpCEzQEejDj7GYCv5FVyjwo9EUA5I5cBLDrykwHKLVK5T2cWuf1821vwVjQyTfrnTVdSqroJqtYt7G6OcEMaS7X602cRi42yQUTvbamtcSLyl8e7_PX4BUv-SrQ</recordid><startdate>19920901</startdate><enddate>19920901</enddate><creator>Penfold, Robert J.</creator><creator>Pemberton, John M.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>C1K</scope></search><sort><creationdate>19920901</creationdate><title>An improved suicide vector for construction of chromosomal insertion mutations in bacteria</title><author>Penfold, Robert J. ; Pemberton, John M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c534t-446636751247324675d559f12a37732237cea35cd42b6a5a559d27bc96d9babd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Bacterial Proteins - genetics</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Chromosomes, Bacterial</topic><topic>DNA Helicases</topic><topic>DNA, Recombinant - genetics</topic><topic>DNA-Binding Proteins</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Genetic Vectors - genetics</topic><topic>Methods. Procedures. Technologies</topic><topic>mobilization</topic><topic>Mutagenesis, Insertional - methods</topic><topic>pir gene</topic><topic>R6K replicon</topic><topic>Recombinant DNA</topic><topic>Replicon - genetics</topic><topic>Rhodobacter sphaeroides</topic><topic>Rhodobacter sphaeroides - genetics</topic><topic>site-directed mutagenesis</topic><topic>Trans-Activators</topic><topic>Vectors (cloning, transfer, expression). Insertion sequences and transposons</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Penfold, Robert J.</creatorcontrib><creatorcontrib>Pemberton, John M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Penfold, Robert J.</au><au>Pemberton, John M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An improved suicide vector for construction of chromosomal insertion mutations in bacteria</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1992-09-01</date><risdate>1992</risdate><volume>118</volume><issue>1</issue><spage>145</spage><epage>146</epage><pages>145-146</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><coden>GENED6</coden><abstract>We have constructed an R6K-based suicide vector (pJP5603) that requires a
trans supply of the
pir-encoded π protein of plasmid R6K for replication. Therefore, efficient plasmid suicide results upon transfer to bacteria not harbouring
pir. The 3.1-kb vector encodes kanamycin resistance and is mobilizable. When used in conjunction with a JM109 strain carrying
pir, it has nine unique restriction sites available for α-complementation cloning. Vector functionality was demonstrated in
Rhodobacter sphaeroides.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>1511879</pmid><doi>10.1016/0378-1119(92)90263-O</doi><tpages>2</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1119 |
| ispartof | Gene, 1992-09, Vol.118 (1), p.145-146 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16301366 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Bacterial Proteins - genetics Biological and medical sciences Biotechnology Chromosomes, Bacterial DNA Helicases DNA, Recombinant - genetics DNA-Binding Proteins Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Genetic Vectors - genetics Methods. Procedures. Technologies mobilization Mutagenesis, Insertional - methods pir gene R6K replicon Recombinant DNA Replicon - genetics Rhodobacter sphaeroides Rhodobacter sphaeroides - genetics site-directed mutagenesis Trans-Activators Vectors (cloning, transfer, expression). Insertion sequences and transposons |
| title | An improved suicide vector for construction of chromosomal insertion mutations in bacteria |
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