Effects of the calcium channel blockers Phα1β and ω-conotoxin MVIIA on capsaicin and acetic acid-induced visceral nociception in mice
The effects of intrathecal administration of the toxins Phα1β and ω-conotoxin MVIIA were investigated in visceral nociception induced by an intraperitoneal injection of acetic acid and an intracolonic application of capsaicin. The pretreatments for 2h with the toxins reduced the number of writhes or...
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| Veröffentlicht in: | Pharmacology, biochemistry and behavior biochemistry and behavior, 2014-11, Vol.126, p.97-102 |
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| creator | Diniz, Danuza Montijo de Souza, Alessandra Hubner Pereira, Elizete Maria Rita da Silva, Juliana Figueira Rigo, Flavia Karine Romano‐Silva, Marco Aurélio Binda, Nancy Castro, Célio J. Cordeiro, Marta Nascimento Ferreira, Juliano Gomez, Marcus Vinicius |
| description | The effects of intrathecal administration of the toxins Phα1β and ω-conotoxin MVIIA were investigated in visceral nociception induced by an intraperitoneal injection of acetic acid and an intracolonic application of capsaicin. The pretreatments for 2h with the toxins reduced the number of writhes or nociceptive behaviors compared with the control mice. Phα1β administration resulted in an Imax of 84±6 and an ID50 of 12 (5–27), and ω-conotoxin MVIIA resulted in an Imax of 82±9 and an ID50 of 11 (4–35) in the contortions induced by the intraperitoneal injection of acetic acid. The administration of Phα1β resulted in an Imax of 64±4 and an ID50 of 18 (9–38), and ω-conotoxin MVIIA resulted in an Imax of 71±9 and an ID50 of 9 (1–83) in the contortions induced by intracolonic capsaicin administration. Phα1β (100/site) or ω-conotoxin MVIIA (30pmol/site) pretreatments caused a reduction in CSF glutamate release in mice intraperitoneally injected with acetic acid or treated with intracolonic capsaicin. The toxin pretreatments reduced the ROS levels induced by intraperitoneal acetic acid injection. Phα1β, but not ω-conotoxin MVIIA, reduced significantly the ROS levels induced by intracolonic capsaicin administration.
Perspective: Phα1β is a ω-toxin with high therapeutic index and a broader action on calcium channels. It shows analgesic effect in several rodents' models of pain, including visceral pain, suggesting that this toxin has the potential to be used in clinical setting as a drug in the control of persistent pathological pain.
•Toxins calcium channel blockers reduced visceral nociception in mice.•Toxins reduced acetic acid induced visceral nociception in mice.•Toxins reduced capsaicin induced intracolonic visceral nociception in mice.•Toxins reduced CSF glutamate in visceral and intraperitoneal nociception in mice.•Toxins reduced CSF ROS levels in visceral and intraperitoneal nociception in mice. |
| doi_str_mv | 10.1016/j.pbb.2014.09.017 |
| format | Article |
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Perspective: Phα1β is a ω-toxin with high therapeutic index and a broader action on calcium channels. It shows analgesic effect in several rodents' models of pain, including visceral pain, suggesting that this toxin has the potential to be used in clinical setting as a drug in the control of persistent pathological pain.
•Toxins calcium channel blockers reduced visceral nociception in mice.•Toxins reduced acetic acid induced visceral nociception in mice.•Toxins reduced capsaicin induced intracolonic visceral nociception in mice.•Toxins reduced CSF glutamate in visceral and intraperitoneal nociception in mice.•Toxins reduced CSF ROS levels in visceral and intraperitoneal nociception in mice.</description><identifier>ISSN: 0091-3057</identifier><identifier>EISSN: 1873-5177</identifier><identifier>DOI: 10.1016/j.pbb.2014.09.017</identifier><identifier>PMID: 25268314</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Acetic Acid - administration & dosage ; Acetic Acid - pharmacology ; Administration, Rectal ; Animals ; Calcium channel blockers ; Calcium Channel Blockers - pharmacology ; Capsaicin - administration & dosage ; Capsaicin - pharmacology ; Dose-Response Relationship, Drug ; Glutamate ; Glutamic Acid - cerebrospinal fluid ; Injections, Spinal ; Male ; Mice ; Nociception - drug effects ; omega-Conotoxins - pharmacology ; Reactive Oxygen Species - cerebrospinal fluid ; ROS ; Spider Venoms - pharmacology ; Toxins ; Visceral nociception</subject><ispartof>Pharmacology, biochemistry and behavior, 2014-11, Vol.126, p.97-102</ispartof><rights>2014 Elsevier Inc.</rights><rights>Copyright © 2014 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c268t-2c6c270a2edd79f234838b5a4a54fd9b9eb4aa5f73d58fd516bc4fd11a0ddcdd3</citedby><cites>FETCH-LOGICAL-c268t-2c6c270a2edd79f234838b5a4a54fd9b9eb4aa5f73d58fd516bc4fd11a0ddcdd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S009130571400269X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25268314$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Diniz, Danuza Montijo</creatorcontrib><creatorcontrib>de Souza, Alessandra Hubner</creatorcontrib><creatorcontrib>Pereira, Elizete Maria Rita</creatorcontrib><creatorcontrib>da Silva, Juliana Figueira</creatorcontrib><creatorcontrib>Rigo, Flavia Karine</creatorcontrib><creatorcontrib>Romano‐Silva, Marco Aurélio</creatorcontrib><creatorcontrib>Binda, Nancy</creatorcontrib><creatorcontrib>Castro, Célio J.</creatorcontrib><creatorcontrib>Cordeiro, Marta Nascimento</creatorcontrib><creatorcontrib>Ferreira, Juliano</creatorcontrib><creatorcontrib>Gomez, Marcus Vinicius</creatorcontrib><title>Effects of the calcium channel blockers Phα1β and ω-conotoxin MVIIA on capsaicin and acetic acid-induced visceral nociception in mice</title><title>Pharmacology, biochemistry and behavior</title><addtitle>Pharmacol Biochem Behav</addtitle><description>The effects of intrathecal administration of the toxins Phα1β and ω-conotoxin MVIIA were investigated in visceral nociception induced by an intraperitoneal injection of acetic acid and an intracolonic application of capsaicin. The pretreatments for 2h with the toxins reduced the number of writhes or nociceptive behaviors compared with the control mice. Phα1β administration resulted in an Imax of 84±6 and an ID50 of 12 (5–27), and ω-conotoxin MVIIA resulted in an Imax of 82±9 and an ID50 of 11 (4–35) in the contortions induced by the intraperitoneal injection of acetic acid. The administration of Phα1β resulted in an Imax of 64±4 and an ID50 of 18 (9–38), and ω-conotoxin MVIIA resulted in an Imax of 71±9 and an ID50 of 9 (1–83) in the contortions induced by intracolonic capsaicin administration. Phα1β (100/site) or ω-conotoxin MVIIA (30pmol/site) pretreatments caused a reduction in CSF glutamate release in mice intraperitoneally injected with acetic acid or treated with intracolonic capsaicin. The toxin pretreatments reduced the ROS levels induced by intraperitoneal acetic acid injection. Phα1β, but not ω-conotoxin MVIIA, reduced significantly the ROS levels induced by intracolonic capsaicin administration.
Perspective: Phα1β is a ω-toxin with high therapeutic index and a broader action on calcium channels. It shows analgesic effect in several rodents' models of pain, including visceral pain, suggesting that this toxin has the potential to be used in clinical setting as a drug in the control of persistent pathological pain.
•Toxins calcium channel blockers reduced visceral nociception in mice.•Toxins reduced acetic acid induced visceral nociception in mice.•Toxins reduced capsaicin induced intracolonic visceral nociception in mice.•Toxins reduced CSF glutamate in visceral and intraperitoneal nociception in mice.•Toxins reduced CSF ROS levels in visceral and intraperitoneal nociception in mice.</description><subject>Acetic Acid - administration & dosage</subject><subject>Acetic Acid - pharmacology</subject><subject>Administration, Rectal</subject><subject>Animals</subject><subject>Calcium channel blockers</subject><subject>Calcium Channel Blockers - pharmacology</subject><subject>Capsaicin - administration & dosage</subject><subject>Capsaicin - pharmacology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Glutamate</subject><subject>Glutamic Acid - cerebrospinal fluid</subject><subject>Injections, Spinal</subject><subject>Male</subject><subject>Mice</subject><subject>Nociception - drug effects</subject><subject>omega-Conotoxins - pharmacology</subject><subject>Reactive Oxygen Species - cerebrospinal fluid</subject><subject>ROS</subject><subject>Spider Venoms - pharmacology</subject><subject>Toxins</subject><subject>Visceral nociception</subject><issn>0091-3057</issn><issn>1873-5177</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc9u1DAQxi0EokvhAbggH7kk2M4fJ-JUVQVWKioH4Go547HWS2IHO6ngDeBx4EHaV6pX23LkNJrx7_s0no-Ql5yVnPH2zb6ch6EUjNcl60vG5SOy4Z2sioZL-ZhsGOt5UbFGnpBnKe0ZY7Vo5VNyIhrRdhWvN-TXhbUIS6LB0mWHFPQIbp0o7LT3ONJhDPANY6Kfdjd_-M1fqr2ht78LCD4s4Yfz9OPX7faMBp-lc9IO8ujAaMDFQS7OFM6bFdDQa5cAox6pD-AA58VlWean3DwnT6weE764r6fky7uLz-cfisur99vzs8sC8spLIaAFIZkWaIzsrajqruqGRte6qa3phx6HWuvGyso0nTUNbwfID5xrZgwYU52S10ffOYbvK6ZFTYetxlF7DGtSvBV9L2UleUb5EYUYUopo1RzdpONPxZk6BKD2KgegDgEo1qscQNa8urdfhwnNP8XDxTPw9ghg_uS1w6gSOPT5PC7mIJQJ7j_2d6n5mic</recordid><startdate>201411</startdate><enddate>201411</enddate><creator>Diniz, Danuza Montijo</creator><creator>de Souza, Alessandra Hubner</creator><creator>Pereira, Elizete Maria Rita</creator><creator>da Silva, Juliana Figueira</creator><creator>Rigo, Flavia Karine</creator><creator>Romano‐Silva, Marco Aurélio</creator><creator>Binda, Nancy</creator><creator>Castro, Célio J.</creator><creator>Cordeiro, Marta Nascimento</creator><creator>Ferreira, Juliano</creator><creator>Gomez, Marcus Vinicius</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201411</creationdate><title>Effects of the calcium channel blockers Phα1β and ω-conotoxin MVIIA on capsaicin and acetic acid-induced visceral nociception in mice</title><author>Diniz, Danuza Montijo ; de Souza, Alessandra Hubner ; Pereira, Elizete Maria Rita ; da Silva, Juliana Figueira ; Rigo, Flavia Karine ; Romano‐Silva, Marco Aurélio ; Binda, Nancy ; Castro, Célio J. ; Cordeiro, Marta Nascimento ; Ferreira, Juliano ; Gomez, Marcus Vinicius</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c268t-2c6c270a2edd79f234838b5a4a54fd9b9eb4aa5f73d58fd516bc4fd11a0ddcdd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Acetic Acid - administration & dosage</topic><topic>Acetic Acid - pharmacology</topic><topic>Administration, Rectal</topic><topic>Animals</topic><topic>Calcium channel blockers</topic><topic>Calcium Channel Blockers - pharmacology</topic><topic>Capsaicin - administration & dosage</topic><topic>Capsaicin - pharmacology</topic><topic>Dose-Response Relationship, Drug</topic><topic>Glutamate</topic><topic>Glutamic Acid - cerebrospinal fluid</topic><topic>Injections, Spinal</topic><topic>Male</topic><topic>Mice</topic><topic>Nociception - drug effects</topic><topic>omega-Conotoxins - pharmacology</topic><topic>Reactive Oxygen Species - cerebrospinal fluid</topic><topic>ROS</topic><topic>Spider Venoms - pharmacology</topic><topic>Toxins</topic><topic>Visceral nociception</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Diniz, Danuza Montijo</creatorcontrib><creatorcontrib>de Souza, Alessandra Hubner</creatorcontrib><creatorcontrib>Pereira, Elizete Maria Rita</creatorcontrib><creatorcontrib>da Silva, Juliana Figueira</creatorcontrib><creatorcontrib>Rigo, Flavia Karine</creatorcontrib><creatorcontrib>Romano‐Silva, Marco Aurélio</creatorcontrib><creatorcontrib>Binda, Nancy</creatorcontrib><creatorcontrib>Castro, Célio J.</creatorcontrib><creatorcontrib>Cordeiro, Marta Nascimento</creatorcontrib><creatorcontrib>Ferreira, Juliano</creatorcontrib><creatorcontrib>Gomez, Marcus Vinicius</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Pharmacology, biochemistry and behavior</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Diniz, Danuza Montijo</au><au>de Souza, Alessandra Hubner</au><au>Pereira, Elizete Maria Rita</au><au>da Silva, Juliana Figueira</au><au>Rigo, Flavia Karine</au><au>Romano‐Silva, Marco Aurélio</au><au>Binda, Nancy</au><au>Castro, Célio J.</au><au>Cordeiro, Marta Nascimento</au><au>Ferreira, Juliano</au><au>Gomez, Marcus Vinicius</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of the calcium channel blockers Phα1β and ω-conotoxin MVIIA on capsaicin and acetic acid-induced visceral nociception in mice</atitle><jtitle>Pharmacology, biochemistry and behavior</jtitle><addtitle>Pharmacol Biochem Behav</addtitle><date>2014-11</date><risdate>2014</risdate><volume>126</volume><spage>97</spage><epage>102</epage><pages>97-102</pages><issn>0091-3057</issn><eissn>1873-5177</eissn><abstract>The effects of intrathecal administration of the toxins Phα1β and ω-conotoxin MVIIA were investigated in visceral nociception induced by an intraperitoneal injection of acetic acid and an intracolonic application of capsaicin. The pretreatments for 2h with the toxins reduced the number of writhes or nociceptive behaviors compared with the control mice. Phα1β administration resulted in an Imax of 84±6 and an ID50 of 12 (5–27), and ω-conotoxin MVIIA resulted in an Imax of 82±9 and an ID50 of 11 (4–35) in the contortions induced by the intraperitoneal injection of acetic acid. The administration of Phα1β resulted in an Imax of 64±4 and an ID50 of 18 (9–38), and ω-conotoxin MVIIA resulted in an Imax of 71±9 and an ID50 of 9 (1–83) in the contortions induced by intracolonic capsaicin administration. Phα1β (100/site) or ω-conotoxin MVIIA (30pmol/site) pretreatments caused a reduction in CSF glutamate release in mice intraperitoneally injected with acetic acid or treated with intracolonic capsaicin. The toxin pretreatments reduced the ROS levels induced by intraperitoneal acetic acid injection. Phα1β, but not ω-conotoxin MVIIA, reduced significantly the ROS levels induced by intracolonic capsaicin administration.
Perspective: Phα1β is a ω-toxin with high therapeutic index and a broader action on calcium channels. It shows analgesic effect in several rodents' models of pain, including visceral pain, suggesting that this toxin has the potential to be used in clinical setting as a drug in the control of persistent pathological pain.
•Toxins calcium channel blockers reduced visceral nociception in mice.•Toxins reduced acetic acid induced visceral nociception in mice.•Toxins reduced capsaicin induced intracolonic visceral nociception in mice.•Toxins reduced CSF glutamate in visceral and intraperitoneal nociception in mice.•Toxins reduced CSF ROS levels in visceral and intraperitoneal nociception in mice.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>25268314</pmid><doi>10.1016/j.pbb.2014.09.017</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0091-3057 |
| ispartof | Pharmacology, biochemistry and behavior, 2014-11, Vol.126, p.97-102 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Acetic Acid - administration & dosage Acetic Acid - pharmacology Administration, Rectal Animals Calcium channel blockers Calcium Channel Blockers - pharmacology Capsaicin - administration & dosage Capsaicin - pharmacology Dose-Response Relationship, Drug Glutamate Glutamic Acid - cerebrospinal fluid Injections, Spinal Male Mice Nociception - drug effects omega-Conotoxins - pharmacology Reactive Oxygen Species - cerebrospinal fluid ROS Spider Venoms - pharmacology Toxins Visceral nociception |
| title | Effects of the calcium channel blockers Phα1β and ω-conotoxin MVIIA on capsaicin and acetic acid-induced visceral nociception in mice |
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