Growth associated protein (GAP-43): Cloning and the development of a sensitive ELISA for neurological disorders
Abstract GAP-43 has been studied in the rodent and mammalian brain and shown to be present specifically in areas undergoing axonal elongation and synapse formation. GAP-43 was cloned using the baculovirus expression system and purified. A sandwich ELISA was developed using the recombinant GAP-43 as...
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| Veröffentlicht in: | Journal of neuroimmunology 2014-11, Vol.276 (1), p.18-23 |
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| container_title | Journal of neuroimmunology |
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| creator | Gnanapavan, Sharmilee Yousaf, Nasim Heywood, Wendy Grant, Donna Mills, Kevin Chernajovsky, Yuti Keir, Geoff Giovannoni, Gavin |
| description | Abstract GAP-43 has been studied in the rodent and mammalian brain and shown to be present specifically in areas undergoing axonal elongation and synapse formation. GAP-43 was cloned using the baculovirus expression system and purified. A sandwich ELISA was developed using the recombinant GAP-43 as standard and validated. CSF GAP-43 levels were analysed in benign intracranial hypertension, movement disorders, multiple sclerosis, neuropathy, CNS infections, motor neuron disease, and headache (neurological controls). GAP-43 levels were low in all disorders analysed (in particular motor neuron disease; p = 0.001, and movement disorders and multiple sclerosis; p < 0.0001) compared to controls, aside from CNS infections. GAP-43 is preferentially reduced in the CSF of neurological disorders associated with neurodegeneration. |
| doi_str_mv | 10.1016/j.jneuroim.2014.07.008 |
| format | Article |
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GAP-43 was cloned using the baculovirus expression system and purified. A sandwich ELISA was developed using the recombinant GAP-43 as standard and validated. CSF GAP-43 levels were analysed in benign intracranial hypertension, movement disorders, multiple sclerosis, neuropathy, CNS infections, motor neuron disease, and headache (neurological controls). GAP-43 levels were low in all disorders analysed (in particular motor neuron disease; p = 0.001, and movement disorders and multiple sclerosis; p < 0.0001) compared to controls, aside from CNS infections. GAP-43 is preferentially reduced in the CSF of neurological disorders associated with neurodegeneration.</description><identifier>ISSN: 0165-5728</identifier><identifier>EISSN: 1872-8421</identifier><identifier>DOI: 10.1016/j.jneuroim.2014.07.008</identifier><identifier>PMID: 25175067</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Allergy and Immunology ; Animals ; Biomarker ; Cloning, Organism ; CSF ; ELISA ; Enzyme-Linked Immunosorbent Assay - methods ; GAP-43 ; GAP-43 Protein - genetics ; GAP-43 Protein - metabolism ; Humans ; Mice ; Nervous System Diseases - cerebrospinal fluid ; Nervous System Diseases - diagnosis ; Neurology ; Neuroplasticity</subject><ispartof>Journal of neuroimmunology, 2014-11, Vol.276 (1), p.18-23</ispartof><rights>2014</rights><rights>Copyright © 2014. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c493t-91f71bb6149b18abd637f33ec82f826a04ff77ebc92f8451eb35b43ac49af6dd3</citedby><cites>FETCH-LOGICAL-c493t-91f71bb6149b18abd637f33ec82f826a04ff77ebc92f8451eb35b43ac49af6dd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jneuroim.2014.07.008$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25175067$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gnanapavan, Sharmilee</creatorcontrib><creatorcontrib>Yousaf, Nasim</creatorcontrib><creatorcontrib>Heywood, Wendy</creatorcontrib><creatorcontrib>Grant, Donna</creatorcontrib><creatorcontrib>Mills, Kevin</creatorcontrib><creatorcontrib>Chernajovsky, Yuti</creatorcontrib><creatorcontrib>Keir, Geoff</creatorcontrib><creatorcontrib>Giovannoni, Gavin</creatorcontrib><title>Growth associated protein (GAP-43): Cloning and the development of a sensitive ELISA for neurological disorders</title><title>Journal of neuroimmunology</title><addtitle>J Neuroimmunol</addtitle><description>Abstract GAP-43 has been studied in the rodent and mammalian brain and shown to be present specifically in areas undergoing axonal elongation and synapse formation. 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GAP-43 is preferentially reduced in the CSF of neurological disorders associated with neurodegeneration.</description><subject>Allergy and Immunology</subject><subject>Animals</subject><subject>Biomarker</subject><subject>Cloning, Organism</subject><subject>CSF</subject><subject>ELISA</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>GAP-43</subject><subject>GAP-43 Protein - genetics</subject><subject>GAP-43 Protein - metabolism</subject><subject>Humans</subject><subject>Mice</subject><subject>Nervous System Diseases - cerebrospinal fluid</subject><subject>Nervous System Diseases - diagnosis</subject><subject>Neurology</subject><subject>Neuroplasticity</subject><issn>0165-5728</issn><issn>1872-8421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhiMEokvhL1Q-lkOCvxInHBCrVdlWWgmkwtly7HHrkLUXO1nUf4_TbTlw4WSN9LwznmeK4oLgimDSfBiqwcMcg9tXFBNeYVFh3L4oVqQVtGw5JS-LVQbrsha0PSvepDRgTGrGu9fFGa2JqHEjVkXYxvB7ukcqpaCdmsCgQwwTOI8ut-tvJWfvP6LNGLzzd0h5g6Z7QAaOMIbDHvyEgkUKJfDJTe4I6Gp3c7tGNkT0-L0x3DmtRmRcCtFATG-LV1aNCd49vefFjy9X3zfX5e7r9maz3pWad2wqO2IF6fuG8K4nrepNw4RlDHRLbUsbhbm1QkCvu1zzmkDP6p4zldPKNsaw8-Ly1Ddv82uGNMm9SxrGUXkIc5KkoV0nmhaLjDYnVMeQUgQrD9HtVXyQBMtFthzks2y5yJZYyCw7By-eZsz9Hszf2LPdDHw-AZA3PTqIMmkHXoNxEfQkTXD_n_HpnxZ6dH5x-hMeIA1hjj57lEQmKrG8XU6-XJxwjCllHfsDCoupSw</recordid><startdate>20141115</startdate><enddate>20141115</enddate><creator>Gnanapavan, Sharmilee</creator><creator>Yousaf, Nasim</creator><creator>Heywood, Wendy</creator><creator>Grant, Donna</creator><creator>Mills, Kevin</creator><creator>Chernajovsky, Yuti</creator><creator>Keir, Geoff</creator><creator>Giovannoni, Gavin</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20141115</creationdate><title>Growth associated protein (GAP-43): Cloning and the development of a sensitive ELISA for neurological disorders</title><author>Gnanapavan, Sharmilee ; Yousaf, Nasim ; Heywood, Wendy ; Grant, Donna ; Mills, Kevin ; Chernajovsky, Yuti ; Keir, Geoff ; Giovannoni, Gavin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-91f71bb6149b18abd637f33ec82f826a04ff77ebc92f8451eb35b43ac49af6dd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Allergy and Immunology</topic><topic>Animals</topic><topic>Biomarker</topic><topic>Cloning, Organism</topic><topic>CSF</topic><topic>ELISA</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>GAP-43</topic><topic>GAP-43 Protein - genetics</topic><topic>GAP-43 Protein - metabolism</topic><topic>Humans</topic><topic>Mice</topic><topic>Nervous System Diseases - cerebrospinal fluid</topic><topic>Nervous System Diseases - diagnosis</topic><topic>Neurology</topic><topic>Neuroplasticity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gnanapavan, Sharmilee</creatorcontrib><creatorcontrib>Yousaf, Nasim</creatorcontrib><creatorcontrib>Heywood, Wendy</creatorcontrib><creatorcontrib>Grant, Donna</creatorcontrib><creatorcontrib>Mills, Kevin</creatorcontrib><creatorcontrib>Chernajovsky, Yuti</creatorcontrib><creatorcontrib>Keir, Geoff</creatorcontrib><creatorcontrib>Giovannoni, Gavin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuroimmunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gnanapavan, Sharmilee</au><au>Yousaf, Nasim</au><au>Heywood, Wendy</au><au>Grant, Donna</au><au>Mills, Kevin</au><au>Chernajovsky, Yuti</au><au>Keir, Geoff</au><au>Giovannoni, Gavin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Growth associated protein (GAP-43): Cloning and the development of a sensitive ELISA for neurological disorders</atitle><jtitle>Journal of neuroimmunology</jtitle><addtitle>J Neuroimmunol</addtitle><date>2014-11-15</date><risdate>2014</risdate><volume>276</volume><issue>1</issue><spage>18</spage><epage>23</epage><pages>18-23</pages><issn>0165-5728</issn><eissn>1872-8421</eissn><abstract>Abstract GAP-43 has been studied in the rodent and mammalian brain and shown to be present specifically in areas undergoing axonal elongation and synapse formation. 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| identifier | ISSN: 0165-5728 |
| ispartof | Journal of neuroimmunology, 2014-11, Vol.276 (1), p.18-23 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Allergy and Immunology Animals Biomarker Cloning, Organism CSF ELISA Enzyme-Linked Immunosorbent Assay - methods GAP-43 GAP-43 Protein - genetics GAP-43 Protein - metabolism Humans Mice Nervous System Diseases - cerebrospinal fluid Nervous System Diseases - diagnosis Neurology Neuroplasticity |
| title | Growth associated protein (GAP-43): Cloning and the development of a sensitive ELISA for neurological disorders |
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