Antihypertensive effect of the bovine casein-derived peptide Met-Lys-Pro
•The bovine casein-derived peptide Met-Lys-Pro (MKP) was examined in vitro and in vivo.•MKP showed angiotensin I-converting enzyme (ACE)-inhibitory activity in vitro.•Intact MKP could be absorbed into the plasma by the oral administration.•The MKP suppressed (and delayed) angiotensin II-dependent va...
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| Veröffentlicht in: | Food chemistry 2015-04, Vol.172, p.441-446 |
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| creator | Yamada, Akio Sakurai, Takuma Ochi, Daisuke Mitsuyama, Eri Yamauchi, Koji Abe, Fumiaki |
| description | •The bovine casein-derived peptide Met-Lys-Pro (MKP) was examined in vitro and in vivo.•MKP showed angiotensin I-converting enzyme (ACE)-inhibitory activity in vitro.•Intact MKP could be absorbed into the plasma by the oral administration.•The MKP suppressed (and delayed) angiotensin II-dependent vasoconstriction.•The single-dose or daily administration of MKP to SHRs lowered blood pressure.
The antihypertensive effect of the bovine casein-derived peptide Met-Lys-Pro (MKP) was examined in vitro and in vivo. MKP showed angiotensin I-converting enzyme (ACE)-inhibitory activity in vitro (IC50=0.43μM). An in vivo kinetics study using radiolabeled Met-[1-14C]Lys-Pro (14C-MKP) showed that orally administered 14C-MKP to spontaneously hypertensive rats (SHRs) was absorbed and moved into the plasma. In vitro vasoconstriction of thoracic aorta preparations, which was induced by adding angiotensin I, was reduced by prior exposure of MKP. A single oral dose of MKP lowered systolic blood pressure (SBP) of SHRs, and repeated oral administration of MKP for 28days significantly lowered SBP of SHRs. The results obtained in the present study suggest that orally administrated MKP can be absorbed into the plasma and its ACE-inhibitory activity may contribute to induce the antihypertensive effect in vivo. |
| doi_str_mv | 10.1016/j.foodchem.2014.09.098 |
| format | Article |
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The antihypertensive effect of the bovine casein-derived peptide Met-Lys-Pro (MKP) was examined in vitro and in vivo. MKP showed angiotensin I-converting enzyme (ACE)-inhibitory activity in vitro (IC50=0.43μM). An in vivo kinetics study using radiolabeled Met-[1-14C]Lys-Pro (14C-MKP) showed that orally administered 14C-MKP to spontaneously hypertensive rats (SHRs) was absorbed and moved into the plasma. In vitro vasoconstriction of thoracic aorta preparations, which was induced by adding angiotensin I, was reduced by prior exposure of MKP. A single oral dose of MKP lowered systolic blood pressure (SBP) of SHRs, and repeated oral administration of MKP for 28days significantly lowered SBP of SHRs. The results obtained in the present study suggest that orally administrated MKP can be absorbed into the plasma and its ACE-inhibitory activity may contribute to induce the antihypertensive effect in vivo.</description><identifier>ISSN: 0308-8146</identifier><identifier>EISSN: 1873-7072</identifier><identifier>DOI: 10.1016/j.foodchem.2014.09.098</identifier><identifier>PMID: 25442576</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>14C-labeled peptide ; ACE-inhibitory peptide ; Administration, Oral ; Angiotensin I-converting enzyme ; Angiotensin-Converting Enzyme Inhibitors - chemistry ; Angiotensin-Converting Enzyme Inhibitors - pharmacology ; Animals ; Antihypertensive Agents - pharmacology ; Bioavailability ; Blood Pressure - drug effects ; Casein ; Caseins - chemistry ; Caseins - pharmacology ; Dose-Response Relationship, Drug ; Hypertension ; Male ; Peptides - chemistry ; Rats ; Rats, Inbred SHR ; Rats, Wistar ; Vasoconstriction - drug effects</subject><ispartof>Food chemistry, 2015-04, Vol.172, p.441-446</ispartof><rights>2014 Elsevier Ltd</rights><rights>Copyright © 2014 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c487t-2186887fb88a95fe2fcc6768dbaf5c271964a043a2aa6742f247ae5d693f031a3</citedby><cites>FETCH-LOGICAL-c487t-2186887fb88a95fe2fcc6768dbaf5c271964a043a2aa6742f247ae5d693f031a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0308814614014745$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25442576$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yamada, Akio</creatorcontrib><creatorcontrib>Sakurai, Takuma</creatorcontrib><creatorcontrib>Ochi, Daisuke</creatorcontrib><creatorcontrib>Mitsuyama, Eri</creatorcontrib><creatorcontrib>Yamauchi, Koji</creatorcontrib><creatorcontrib>Abe, Fumiaki</creatorcontrib><title>Antihypertensive effect of the bovine casein-derived peptide Met-Lys-Pro</title><title>Food chemistry</title><addtitle>Food Chem</addtitle><description>•The bovine casein-derived peptide Met-Lys-Pro (MKP) was examined in vitro and in vivo.•MKP showed angiotensin I-converting enzyme (ACE)-inhibitory activity in vitro.•Intact MKP could be absorbed into the plasma by the oral administration.•The MKP suppressed (and delayed) angiotensin II-dependent vasoconstriction.•The single-dose or daily administration of MKP to SHRs lowered blood pressure.
The antihypertensive effect of the bovine casein-derived peptide Met-Lys-Pro (MKP) was examined in vitro and in vivo. MKP showed angiotensin I-converting enzyme (ACE)-inhibitory activity in vitro (IC50=0.43μM). An in vivo kinetics study using radiolabeled Met-[1-14C]Lys-Pro (14C-MKP) showed that orally administered 14C-MKP to spontaneously hypertensive rats (SHRs) was absorbed and moved into the plasma. In vitro vasoconstriction of thoracic aorta preparations, which was induced by adding angiotensin I, was reduced by prior exposure of MKP. A single oral dose of MKP lowered systolic blood pressure (SBP) of SHRs, and repeated oral administration of MKP for 28days significantly lowered SBP of SHRs. The results obtained in the present study suggest that orally administrated MKP can be absorbed into the plasma and its ACE-inhibitory activity may contribute to induce the antihypertensive effect in vivo.</description><subject>14C-labeled peptide</subject><subject>ACE-inhibitory peptide</subject><subject>Administration, Oral</subject><subject>Angiotensin I-converting enzyme</subject><subject>Angiotensin-Converting Enzyme Inhibitors - chemistry</subject><subject>Angiotensin-Converting Enzyme Inhibitors - pharmacology</subject><subject>Animals</subject><subject>Antihypertensive Agents - pharmacology</subject><subject>Bioavailability</subject><subject>Blood Pressure - drug effects</subject><subject>Casein</subject><subject>Caseins - chemistry</subject><subject>Caseins - pharmacology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Hypertension</subject><subject>Male</subject><subject>Peptides - chemistry</subject><subject>Rats</subject><subject>Rats, Inbred SHR</subject><subject>Rats, Wistar</subject><subject>Vasoconstriction - drug effects</subject><issn>0308-8146</issn><issn>1873-7072</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFKAzEQhoMotlZfoezRy9Ykm02yN0tRK1T0oOeQJhOa0m7WZFvo25vS6lUYmMv3z898CI0JnhBM-MN64kKwZgXbCcWETXCTR16gIZGiKgUW9BINcYVlKQnjA3ST0hpjnFl5jQa0ZozWgg_RfNr2fnXoIPbQJr-HApwD0xfBFf0KimXY-xYKoxP4trQQM2KLDrreWyjeoC8Xh1R-xHCLrpzeJLg77xH6en76nM3LxfvL62y6KA2Toi8pkVxK4ZZS6qZ2QJ0xXHBpl9rVhgrScKYxqzTVmgtGHWVCQ215UzlcEV2N0P3pbhfD9w5Sr7Y-GdhsdAthlxThtGlEzRqeUX5CTQwpRXCqi36r40ERrI4W1Vr9WlRHiwo3eWQOjs8du-UW7F_sV1sGHk8A5E_3HqJKxkNrwPqY5Skb_H8dP-Z4hpI</recordid><startdate>20150401</startdate><enddate>20150401</enddate><creator>Yamada, Akio</creator><creator>Sakurai, Takuma</creator><creator>Ochi, Daisuke</creator><creator>Mitsuyama, Eri</creator><creator>Yamauchi, Koji</creator><creator>Abe, Fumiaki</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150401</creationdate><title>Antihypertensive effect of the bovine casein-derived peptide Met-Lys-Pro</title><author>Yamada, Akio ; Sakurai, Takuma ; Ochi, Daisuke ; Mitsuyama, Eri ; Yamauchi, Koji ; Abe, Fumiaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c487t-2186887fb88a95fe2fcc6768dbaf5c271964a043a2aa6742f247ae5d693f031a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>14C-labeled peptide</topic><topic>ACE-inhibitory peptide</topic><topic>Administration, Oral</topic><topic>Angiotensin I-converting enzyme</topic><topic>Angiotensin-Converting Enzyme Inhibitors - chemistry</topic><topic>Angiotensin-Converting Enzyme Inhibitors - pharmacology</topic><topic>Animals</topic><topic>Antihypertensive Agents - pharmacology</topic><topic>Bioavailability</topic><topic>Blood Pressure - drug effects</topic><topic>Casein</topic><topic>Caseins - chemistry</topic><topic>Caseins - pharmacology</topic><topic>Dose-Response Relationship, Drug</topic><topic>Hypertension</topic><topic>Male</topic><topic>Peptides - chemistry</topic><topic>Rats</topic><topic>Rats, Inbred SHR</topic><topic>Rats, Wistar</topic><topic>Vasoconstriction - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yamada, Akio</creatorcontrib><creatorcontrib>Sakurai, Takuma</creatorcontrib><creatorcontrib>Ochi, Daisuke</creatorcontrib><creatorcontrib>Mitsuyama, Eri</creatorcontrib><creatorcontrib>Yamauchi, Koji</creatorcontrib><creatorcontrib>Abe, Fumiaki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yamada, Akio</au><au>Sakurai, Takuma</au><au>Ochi, Daisuke</au><au>Mitsuyama, Eri</au><au>Yamauchi, Koji</au><au>Abe, Fumiaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antihypertensive effect of the bovine casein-derived peptide Met-Lys-Pro</atitle><jtitle>Food chemistry</jtitle><addtitle>Food Chem</addtitle><date>2015-04-01</date><risdate>2015</risdate><volume>172</volume><spage>441</spage><epage>446</epage><pages>441-446</pages><issn>0308-8146</issn><eissn>1873-7072</eissn><abstract>•The bovine casein-derived peptide Met-Lys-Pro (MKP) was examined in vitro and in vivo.•MKP showed angiotensin I-converting enzyme (ACE)-inhibitory activity in vitro.•Intact MKP could be absorbed into the plasma by the oral administration.•The MKP suppressed (and delayed) angiotensin II-dependent vasoconstriction.•The single-dose or daily administration of MKP to SHRs lowered blood pressure.
The antihypertensive effect of the bovine casein-derived peptide Met-Lys-Pro (MKP) was examined in vitro and in vivo. MKP showed angiotensin I-converting enzyme (ACE)-inhibitory activity in vitro (IC50=0.43μM). An in vivo kinetics study using radiolabeled Met-[1-14C]Lys-Pro (14C-MKP) showed that orally administered 14C-MKP to spontaneously hypertensive rats (SHRs) was absorbed and moved into the plasma. In vitro vasoconstriction of thoracic aorta preparations, which was induced by adding angiotensin I, was reduced by prior exposure of MKP. A single oral dose of MKP lowered systolic blood pressure (SBP) of SHRs, and repeated oral administration of MKP for 28days significantly lowered SBP of SHRs. The results obtained in the present study suggest that orally administrated MKP can be absorbed into the plasma and its ACE-inhibitory activity may contribute to induce the antihypertensive effect in vivo.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>25442576</pmid><doi>10.1016/j.foodchem.2014.09.098</doi><tpages>6</tpages></addata></record> |
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| subjects | 14C-labeled peptide ACE-inhibitory peptide Administration, Oral Angiotensin I-converting enzyme Angiotensin-Converting Enzyme Inhibitors - chemistry Angiotensin-Converting Enzyme Inhibitors - pharmacology Animals Antihypertensive Agents - pharmacology Bioavailability Blood Pressure - drug effects Casein Caseins - chemistry Caseins - pharmacology Dose-Response Relationship, Drug Hypertension Male Peptides - chemistry Rats Rats, Inbred SHR Rats, Wistar Vasoconstriction - drug effects |
| title | Antihypertensive effect of the bovine casein-derived peptide Met-Lys-Pro |
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