Small oral squamous cell carcinomas with nodal lymphogenic metastasis show increased infiltration of M2 polarized macrophages – An immunohistochemical analysis
Abstract Background In solid malignancies the influence of immunological parameters – especially of macrophages – on invasiveness, metastatic potential and prognosis has been shown. There are no studies quantitatively analysing the macrophage polarization in oral squamous cell carcinoma (oscc). The...
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| Veröffentlicht in: | Journal of cranio-maxillo-facial surgery 2014-10, Vol.42 (7), p.1087-1094 |
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| container_title | Journal of cranio-maxillo-facial surgery |
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| creator | Weber, Manuel Büttner-Herold, Maike Hyckel, Peter Moebius, Patrick Distel, Luitpold Ries, Jutta Amann, Kerstin Neukam, Friedrich W Wehrhan, Falk |
| description | Abstract Background In solid malignancies the influence of immunological parameters – especially of macrophages – on invasiveness, metastatic potential and prognosis has been shown. There are no studies quantitatively analysing the macrophage polarization in oral squamous cell carcinoma (oscc). The aim of this study was to correlate macrophage polarization in the epithelial and stromal compartment of oscc with histopathologic parameters. Methods T1 and T2 oscc samples ( n = 34) were used. Automated immunohistochemical staining detected CD68, CD11c, CD163 and MRC1 positive cells. All samples were completely digitalized using whole slide imaging and the number of stained cells per area was assessed quantitatively. Results Primary tumours with lymphogenic metastasis (N+) showed a significantly ( p |
| doi_str_mv | 10.1016/j.jcms.2014.01.035 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1629966096</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>1_s2_0_S1010518214000377</els_id><sourcerecordid>1629966096</sourcerecordid><originalsourceid>FETCH-LOGICAL-c481t-1791d267c98b87bc43b0eb96bcae1364028c7b5dbedafb56641f5068e7bb952b3</originalsourceid><addsrcrecordid>eNp9Us2K1jAULaI44-gLuJAs3bQmaZu2IMIw-AcjLkbXIUlvp6lN0sltHT5XvoNP4Kv5JKZ8owsXQiCX5NxzOefcLHvKaMEoEy-mYjIOC05ZVVBW0LK-l52ytmnzirHufqopo3nNWn6SPUKcKKWCtt3D7IRXdS1qXp9mP6-cmmcSopoJ3mzKhQ2JgfRkVDTWB6eQ3Np1JD70CTMf3DKGa_DWEAerwnQsEhzDLbHeRFAIfaoGO69RrTZ4EgbygZMlzCrab-nTKRPDMqprQPLr-w9y7ol1bvNhtLgGM4KzJk1SXs2HxP04ezCoGeHJ3X2WfX7z-tPFu_zy49v3F-eXualatuas6VjPRWO6VreNNlWpKehOaKOAlaKivDWNrnsNvRp0LUTFhpqKFhqtu5rr8ix7fuRdYrjZAFfpLO5OKA_JFMkE7zohaCcSlB-hSQhihEEu0ToVD5JRuUcjJ7lHI_doJGUyRZOant3xb9pB_7flTxYJ8PIIgKTyq4Uo0VjwBnobwayyD_b__K_-aTez9buVX-AAOIUtJkeTDolcUnm1L8e-G6xKe1E2Tfkbv-i6IA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1629966096</pqid></control><display><type>article</type><title>Small oral squamous cell carcinomas with nodal lymphogenic metastasis show increased infiltration of M2 polarized macrophages – An immunohistochemical analysis</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Weber, Manuel ; Büttner-Herold, Maike ; Hyckel, Peter ; Moebius, Patrick ; Distel, Luitpold ; Ries, Jutta ; Amann, Kerstin ; Neukam, Friedrich W ; Wehrhan, Falk</creator><creatorcontrib>Weber, Manuel ; Büttner-Herold, Maike ; Hyckel, Peter ; Moebius, Patrick ; Distel, Luitpold ; Ries, Jutta ; Amann, Kerstin ; Neukam, Friedrich W ; Wehrhan, Falk</creatorcontrib><description>Abstract Background In solid malignancies the influence of immunological parameters – especially of macrophages – on invasiveness, metastatic potential and prognosis has been shown. There are no studies quantitatively analysing the macrophage polarization in oral squamous cell carcinoma (oscc). The aim of this study was to correlate macrophage polarization in the epithelial and stromal compartment of oscc with histopathologic parameters. Methods T1 and T2 oscc samples ( n = 34) were used. Automated immunohistochemical staining detected CD68, CD11c, CD163 and MRC1 positive cells. All samples were completely digitalized using whole slide imaging and the number of stained cells per area was assessed quantitatively. Results Primary tumours with lymphogenic metastasis (N+) showed a significantly ( p < 0.05) increased count of CD68, CD11c, CD163 and MRC1 positive cells in the epithelial fraction compared to N0 tumours. The ratio of CD163 positive cells (M2 macrophages) to CD68 positive cells (M1 and M2 macrophages) was significantly ( p < 0.05) increased in N+ tumours. Conclusion An increased macrophage infiltration and an increased M2 polarization in primary oral squamous cell carcinomas with lymphogenic metastasis was shown. Macrophages that migrated into the epithelial tumour fraction seem to be of special biological importance. The results indicate a central role of macrophages in the progression of oscc.</description><identifier>ISSN: 1010-5182</identifier><identifier>EISSN: 1878-4119</identifier><identifier>DOI: 10.1016/j.jcms.2014.01.035</identifier><identifier>PMID: 24556525</identifier><language>eng</language><publisher>Scotland: Elsevier Ltd</publisher><subject>Alveolar Process - pathology ; Antigens, CD - analysis ; Antigens, Differentiation, Myelomonocytic - analysis ; Carcinoma, Squamous Cell - pathology ; Carcinoma, Squamous Cell - secondary ; CD11c Antigen - analysis ; Cell Count ; Cell Polarity - immunology ; Chemotaxis - immunology ; Connective Tissue - pathology ; Dentistry ; Epithelium - pathology ; Female ; Humans ; Lymphatic Metastasis - pathology ; Macrophage polarization ; Macrophages - classification ; Macrophages - immunology ; Macrophages - pathology ; Male ; Middle Aged ; Mouth Floor - pathology ; Mouth Neoplasms - pathology ; Neoplasm Grading ; Neoplasm Staging ; Oral cancer ; Oral squamous cell carcinoma ; oscc ; Receptors, Cell Surface - analysis ; Receptors, Immunologic - analysis ; Receptors, Scavenger - analysis ; Surgery ; Tongue Neoplasms - pathology</subject><ispartof>Journal of cranio-maxillo-facial surgery, 2014-10, Vol.42 (7), p.1087-1094</ispartof><rights>European Association for Cranio-Maxillo-Facial Surgery</rights><rights>2014 European Association for Cranio-Maxillo-Facial Surgery</rights><rights>Copyright © 2014 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c481t-1791d267c98b87bc43b0eb96bcae1364028c7b5dbedafb56641f5068e7bb952b3</citedby><cites>FETCH-LOGICAL-c481t-1791d267c98b87bc43b0eb96bcae1364028c7b5dbedafb56641f5068e7bb952b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jcms.2014.01.035$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24556525$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Weber, Manuel</creatorcontrib><creatorcontrib>Büttner-Herold, Maike</creatorcontrib><creatorcontrib>Hyckel, Peter</creatorcontrib><creatorcontrib>Moebius, Patrick</creatorcontrib><creatorcontrib>Distel, Luitpold</creatorcontrib><creatorcontrib>Ries, Jutta</creatorcontrib><creatorcontrib>Amann, Kerstin</creatorcontrib><creatorcontrib>Neukam, Friedrich W</creatorcontrib><creatorcontrib>Wehrhan, Falk</creatorcontrib><title>Small oral squamous cell carcinomas with nodal lymphogenic metastasis show increased infiltration of M2 polarized macrophages – An immunohistochemical analysis</title><title>Journal of cranio-maxillo-facial surgery</title><addtitle>J Craniomaxillofac Surg</addtitle><description>Abstract Background In solid malignancies the influence of immunological parameters – especially of macrophages – on invasiveness, metastatic potential and prognosis has been shown. There are no studies quantitatively analysing the macrophage polarization in oral squamous cell carcinoma (oscc). The aim of this study was to correlate macrophage polarization in the epithelial and stromal compartment of oscc with histopathologic parameters. Methods T1 and T2 oscc samples ( n = 34) were used. Automated immunohistochemical staining detected CD68, CD11c, CD163 and MRC1 positive cells. All samples were completely digitalized using whole slide imaging and the number of stained cells per area was assessed quantitatively. Results Primary tumours with lymphogenic metastasis (N+) showed a significantly ( p < 0.05) increased count of CD68, CD11c, CD163 and MRC1 positive cells in the epithelial fraction compared to N0 tumours. The ratio of CD163 positive cells (M2 macrophages) to CD68 positive cells (M1 and M2 macrophages) was significantly ( p < 0.05) increased in N+ tumours. Conclusion An increased macrophage infiltration and an increased M2 polarization in primary oral squamous cell carcinomas with lymphogenic metastasis was shown. Macrophages that migrated into the epithelial tumour fraction seem to be of special biological importance. The results indicate a central role of macrophages in the progression of oscc.</description><subject>Alveolar Process - pathology</subject><subject>Antigens, CD - analysis</subject><subject>Antigens, Differentiation, Myelomonocytic - analysis</subject><subject>Carcinoma, Squamous Cell - pathology</subject><subject>Carcinoma, Squamous Cell - secondary</subject><subject>CD11c Antigen - analysis</subject><subject>Cell Count</subject><subject>Cell Polarity - immunology</subject><subject>Chemotaxis - immunology</subject><subject>Connective Tissue - pathology</subject><subject>Dentistry</subject><subject>Epithelium - pathology</subject><subject>Female</subject><subject>Humans</subject><subject>Lymphatic Metastasis - pathology</subject><subject>Macrophage polarization</subject><subject>Macrophages - classification</subject><subject>Macrophages - immunology</subject><subject>Macrophages - pathology</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Mouth Floor - pathology</subject><subject>Mouth Neoplasms - pathology</subject><subject>Neoplasm Grading</subject><subject>Neoplasm Staging</subject><subject>Oral cancer</subject><subject>Oral squamous cell carcinoma</subject><subject>oscc</subject><subject>Receptors, Cell Surface - analysis</subject><subject>Receptors, Immunologic - analysis</subject><subject>Receptors, Scavenger - analysis</subject><subject>Surgery</subject><subject>Tongue Neoplasms - pathology</subject><issn>1010-5182</issn><issn>1878-4119</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9Us2K1jAULaI44-gLuJAs3bQmaZu2IMIw-AcjLkbXIUlvp6lN0sltHT5XvoNP4Kv5JKZ8owsXQiCX5NxzOefcLHvKaMEoEy-mYjIOC05ZVVBW0LK-l52ytmnzirHufqopo3nNWn6SPUKcKKWCtt3D7IRXdS1qXp9mP6-cmmcSopoJ3mzKhQ2JgfRkVDTWB6eQ3Np1JD70CTMf3DKGa_DWEAerwnQsEhzDLbHeRFAIfaoGO69RrTZ4EgbygZMlzCrab-nTKRPDMqprQPLr-w9y7ol1bvNhtLgGM4KzJk1SXs2HxP04ezCoGeHJ3X2WfX7z-tPFu_zy49v3F-eXualatuas6VjPRWO6VreNNlWpKehOaKOAlaKivDWNrnsNvRp0LUTFhpqKFhqtu5rr8ix7fuRdYrjZAFfpLO5OKA_JFMkE7zohaCcSlB-hSQhihEEu0ToVD5JRuUcjJ7lHI_doJGUyRZOant3xb9pB_7flTxYJ8PIIgKTyq4Uo0VjwBnobwayyD_b__K_-aTez9buVX-AAOIUtJkeTDolcUnm1L8e-G6xKe1E2Tfkbv-i6IA</recordid><startdate>20141001</startdate><enddate>20141001</enddate><creator>Weber, Manuel</creator><creator>Büttner-Herold, Maike</creator><creator>Hyckel, Peter</creator><creator>Moebius, Patrick</creator><creator>Distel, Luitpold</creator><creator>Ries, Jutta</creator><creator>Amann, Kerstin</creator><creator>Neukam, Friedrich W</creator><creator>Wehrhan, Falk</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20141001</creationdate><title>Small oral squamous cell carcinomas with nodal lymphogenic metastasis show increased infiltration of M2 polarized macrophages – An immunohistochemical analysis</title><author>Weber, Manuel ; Büttner-Herold, Maike ; Hyckel, Peter ; Moebius, Patrick ; Distel, Luitpold ; Ries, Jutta ; Amann, Kerstin ; Neukam, Friedrich W ; Wehrhan, Falk</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c481t-1791d267c98b87bc43b0eb96bcae1364028c7b5dbedafb56641f5068e7bb952b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Alveolar Process - pathology</topic><topic>Antigens, CD - analysis</topic><topic>Antigens, Differentiation, Myelomonocytic - analysis</topic><topic>Carcinoma, Squamous Cell - pathology</topic><topic>Carcinoma, Squamous Cell - secondary</topic><topic>CD11c Antigen - analysis</topic><topic>Cell Count</topic><topic>Cell Polarity - immunology</topic><topic>Chemotaxis - immunology</topic><topic>Connective Tissue - pathology</topic><topic>Dentistry</topic><topic>Epithelium - pathology</topic><topic>Female</topic><topic>Humans</topic><topic>Lymphatic Metastasis - pathology</topic><topic>Macrophage polarization</topic><topic>Macrophages - classification</topic><topic>Macrophages - immunology</topic><topic>Macrophages - pathology</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Mouth Floor - pathology</topic><topic>Mouth Neoplasms - pathology</topic><topic>Neoplasm Grading</topic><topic>Neoplasm Staging</topic><topic>Oral cancer</topic><topic>Oral squamous cell carcinoma</topic><topic>oscc</topic><topic>Receptors, Cell Surface - analysis</topic><topic>Receptors, Immunologic - analysis</topic><topic>Receptors, Scavenger - analysis</topic><topic>Surgery</topic><topic>Tongue Neoplasms - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Weber, Manuel</creatorcontrib><creatorcontrib>Büttner-Herold, Maike</creatorcontrib><creatorcontrib>Hyckel, Peter</creatorcontrib><creatorcontrib>Moebius, Patrick</creatorcontrib><creatorcontrib>Distel, Luitpold</creatorcontrib><creatorcontrib>Ries, Jutta</creatorcontrib><creatorcontrib>Amann, Kerstin</creatorcontrib><creatorcontrib>Neukam, Friedrich W</creatorcontrib><creatorcontrib>Wehrhan, Falk</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cranio-maxillo-facial surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Weber, Manuel</au><au>Büttner-Herold, Maike</au><au>Hyckel, Peter</au><au>Moebius, Patrick</au><au>Distel, Luitpold</au><au>Ries, Jutta</au><au>Amann, Kerstin</au><au>Neukam, Friedrich W</au><au>Wehrhan, Falk</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Small oral squamous cell carcinomas with nodal lymphogenic metastasis show increased infiltration of M2 polarized macrophages – An immunohistochemical analysis</atitle><jtitle>Journal of cranio-maxillo-facial surgery</jtitle><addtitle>J Craniomaxillofac Surg</addtitle><date>2014-10-01</date><risdate>2014</risdate><volume>42</volume><issue>7</issue><spage>1087</spage><epage>1094</epage><pages>1087-1094</pages><issn>1010-5182</issn><eissn>1878-4119</eissn><abstract>Abstract Background In solid malignancies the influence of immunological parameters – especially of macrophages – on invasiveness, metastatic potential and prognosis has been shown. There are no studies quantitatively analysing the macrophage polarization in oral squamous cell carcinoma (oscc). The aim of this study was to correlate macrophage polarization in the epithelial and stromal compartment of oscc with histopathologic parameters. Methods T1 and T2 oscc samples ( n = 34) were used. Automated immunohistochemical staining detected CD68, CD11c, CD163 and MRC1 positive cells. All samples were completely digitalized using whole slide imaging and the number of stained cells per area was assessed quantitatively. Results Primary tumours with lymphogenic metastasis (N+) showed a significantly ( p < 0.05) increased count of CD68, CD11c, CD163 and MRC1 positive cells in the epithelial fraction compared to N0 tumours. The ratio of CD163 positive cells (M2 macrophages) to CD68 positive cells (M1 and M2 macrophages) was significantly ( p < 0.05) increased in N+ tumours. Conclusion An increased macrophage infiltration and an increased M2 polarization in primary oral squamous cell carcinomas with lymphogenic metastasis was shown. Macrophages that migrated into the epithelial tumour fraction seem to be of special biological importance. The results indicate a central role of macrophages in the progression of oscc.</abstract><cop>Scotland</cop><pub>Elsevier Ltd</pub><pmid>24556525</pmid><doi>10.1016/j.jcms.2014.01.035</doi><tpages>8</tpages></addata></record> |
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| subjects | Alveolar Process - pathology Antigens, CD - analysis Antigens, Differentiation, Myelomonocytic - analysis Carcinoma, Squamous Cell - pathology Carcinoma, Squamous Cell - secondary CD11c Antigen - analysis Cell Count Cell Polarity - immunology Chemotaxis - immunology Connective Tissue - pathology Dentistry Epithelium - pathology Female Humans Lymphatic Metastasis - pathology Macrophage polarization Macrophages - classification Macrophages - immunology Macrophages - pathology Male Middle Aged Mouth Floor - pathology Mouth Neoplasms - pathology Neoplasm Grading Neoplasm Staging Oral cancer Oral squamous cell carcinoma oscc Receptors, Cell Surface - analysis Receptors, Immunologic - analysis Receptors, Scavenger - analysis Surgery Tongue Neoplasms - pathology |
| title | Small oral squamous cell carcinomas with nodal lymphogenic metastasis show increased infiltration of M2 polarized macrophages – An immunohistochemical analysis |
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