Sensitive detection of trace amounts of KRAS codon 12 mutations by a fast and novel one-step technique

The objective of this study is to develop a novel and sensitive method for KRAS codon 12 mutation testing. We developed a sensitive one-step real-time digestion-and-block TaqMan probe PCR (RTDB-PCR) technique that uses a thermostable endonuclease and a minor groove binder (MGB) blocker to detect KRA...

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Veröffentlicht in:	Clinical biochemistry 2014-11, Vol.47 (16-17), p.237-242
Hauptverfasser:	Xie, Feifei, Huang, Jie, Qu, Shoufang, Wu, Weili, Jiang, Jun, Wang, Huagui, Wang, Shujuan, Liu, Qi, Zhang, Senlin, Xu, Lizhi, Gao, Shangxian, Zhang, Yunqing, Zhao, Jinyin, Chen, Weijun
Format:	Artikel
Sprache:	eng
Schlagworte:	Codon - genetics Colorectal cancer DNA Mutational Analysis - methods Humans KRAS mutation detection Low-level mutation Mutation ras Proteins - genetics Reproducibility of Results
Online-Zugang:	Volltext
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