Repeated harvest of vacuole-located secondary product from in vitro grown plant cells using 1.02 MHz ultrasound
Beta vulgaris L. (red beetroot) cells from a suspension culture were sonicated with continuous wave ultrasound (1.02 Mhz) for periods ranging from 10 s to 60 s. In general, cells sonicated for greater than or equal to 30 s released vacuole-located pigment into the surrounding medium, whereas cells s...
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| Veröffentlicht in: | Applied microbiology and biotechnology 1990-07, Vol.33 (4), p.448-451 |
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| container_title | Applied microbiology and biotechnology |
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| creator | Kilby, N.J Hunter, C.S |
| description | Beta vulgaris L. (red beetroot) cells from a suspension culture were sonicated with continuous wave ultrasound (1.02 Mhz) for periods ranging from 10 s to 60 s. In general, cells sonicated for greater than or equal to 30 s released vacuole-located pigment into the surrounding medium, whereas cells sonicated for < 30 s and control (unsonicated) cells released no pigment. Sonicated cells were viable as determined using fluorescein diacetate; microscopic examination of a selected population of sonicated cells did not show any signs of obvious structural damage. Sonicated cell samples were subcultured to re-establish actively growing, pigment-competent cell cultures. Cells from such cultures were available for further harvests of the pigment by sonication. For sonication periods greater than or equal to 50 s, repeated cycles of sonication and incubation allowed pigments to be harvested from cells every 7 days during a 21-day culture period. |
| doi_str_mv | 10.1007/BF00176663 |
| format | Article |
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(red beetroot) cells from a suspension culture were sonicated with continuous wave ultrasound (1.02 Mhz) for periods ranging from 10 s to 60 s. In general, cells sonicated for greater than or equal to 30 s released vacuole-located pigment into the surrounding medium, whereas cells sonicated for < 30 s and control (unsonicated) cells released no pigment. Sonicated cells were viable as determined using fluorescein diacetate; microscopic examination of a selected population of sonicated cells did not show any signs of obvious structural damage. Sonicated cell samples were subcultured to re-establish actively growing, pigment-competent cell cultures. Cells from such cultures were available for further harvests of the pigment by sonication. For sonication periods greater than or equal to 50 s, repeated cycles of sonication and incubation allowed pigments to be harvested from cells every 7 days during a 21-day culture period.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/BF00176663</identifier><identifier>CODEN: AMBIDG</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Beta vulgaris ; Biological and medical sciences ; Biotechnology ; cell suspension culture ; disruption ; Eukaryotic cell cultures ; Fundamental and applied biological sciences. Psychology ; isolation ; metabolites ; Methods. Procedures. Technologies ; Miscellaneous ; nondestructive methods ; pigments ; Plant cells and fungal cells ; plant pigments ; suspension culture ; ultrasonic treatment ; ultrasonics ; ultrasound ; vacuoles</subject><ispartof>Applied microbiology and biotechnology, 1990-07, Vol.33 (4), p.448-451</ispartof><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c229t-f238985873a24e97498177e967cfd51d1a848b9d912b430c649cab7e422fa683</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19591165$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Kilby, N.J</creatorcontrib><creatorcontrib>Hunter, C.S</creatorcontrib><title>Repeated harvest of vacuole-located secondary product from in vitro grown plant cells using 1.02 MHz ultrasound</title><title>Applied microbiology and biotechnology</title><description>Beta vulgaris L. (red beetroot) cells from a suspension culture were sonicated with continuous wave ultrasound (1.02 Mhz) for periods ranging from 10 s to 60 s. In general, cells sonicated for greater than or equal to 30 s released vacuole-located pigment into the surrounding medium, whereas cells sonicated for < 30 s and control (unsonicated) cells released no pigment. Sonicated cells were viable as determined using fluorescein diacetate; microscopic examination of a selected population of sonicated cells did not show any signs of obvious structural damage. Sonicated cell samples were subcultured to re-establish actively growing, pigment-competent cell cultures. Cells from such cultures were available for further harvests of the pigment by sonication. For sonication periods greater than or equal to 50 s, repeated cycles of sonication and incubation allowed pigments to be harvested from cells every 7 days during a 21-day culture period.</description><subject>Beta vulgaris</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>cell suspension culture</subject><subject>disruption</subject><subject>Eukaryotic cell cultures</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>isolation</subject><subject>metabolites</subject><subject>Methods. Procedures. Technologies</subject><subject>Miscellaneous</subject><subject>nondestructive methods</subject><subject>pigments</subject><subject>Plant cells and fungal cells</subject><subject>plant pigments</subject><subject>suspension culture</subject><subject>ultrasonic treatment</subject><subject>ultrasonics</subject><subject>ultrasound</subject><subject>vacuoles</subject><issn>0175-7598</issn><issn>1432-0614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><recordid>eNpFkEtLAzEUhYMoWKsb_4DZ6EKYmsdMHkst1goVQet6uM0kdWQ6qclMRX-90Ra6unDvdw_nHITOKRlRQuTN3YQQKoUQ_AANaM5ZRgTND9EgbYtMFlodo5MYPxLFlBAD5F_s2kJnK_wOYWNjh73DGzC9b2zWePN_itb4toLwjdfBV73psAt-hesWb-oueLwM_qvF6wbaDhvbNBH3sW6XmI4Iw0_TH9w3XYDo-7Y6RUcOmmjPdnOI5pP7-XiazZ4fHse3s8wwprvMMa60KpTkwHKrZa4VldJqIY2rClpRULla6EpTtsg5MSLXBhbS5ow5EIoP0dVWNhn-7FOsclXHP2vQWt_HkgqmVaongddb0AQfY7CuXId6laKWlJR_lZb7ShN8uVOFaKBxAVpTx_2HLjSlokjcxZZz4EtYhsS8vTJCOWGScCoI_wXEPX6q</recordid><startdate>199007</startdate><enddate>199007</enddate><creator>Kilby, N.J</creator><creator>Hunter, C.S</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>199007</creationdate><title>Repeated harvest of vacuole-located secondary product from in vitro grown plant cells using 1.02 MHz ultrasound</title><author>Kilby, N.J ; Hunter, C.S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c229t-f238985873a24e97498177e967cfd51d1a848b9d912b430c649cab7e422fa683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Beta vulgaris</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>cell suspension culture</topic><topic>disruption</topic><topic>Eukaryotic cell cultures</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>isolation</topic><topic>metabolites</topic><topic>Methods. Procedures. Technologies</topic><topic>Miscellaneous</topic><topic>nondestructive methods</topic><topic>pigments</topic><topic>Plant cells and fungal cells</topic><topic>plant pigments</topic><topic>suspension culture</topic><topic>ultrasonic treatment</topic><topic>ultrasonics</topic><topic>ultrasound</topic><topic>vacuoles</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kilby, N.J</creatorcontrib><creatorcontrib>Hunter, C.S</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Applied microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kilby, N.J</au><au>Hunter, C.S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Repeated harvest of vacuole-located secondary product from in vitro grown plant cells using 1.02 MHz ultrasound</atitle><jtitle>Applied microbiology and biotechnology</jtitle><date>1990-07</date><risdate>1990</risdate><volume>33</volume><issue>4</issue><spage>448</spage><epage>451</epage><pages>448-451</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><coden>AMBIDG</coden><abstract>Beta vulgaris L. (red beetroot) cells from a suspension culture were sonicated with continuous wave ultrasound (1.02 Mhz) for periods ranging from 10 s to 60 s. In general, cells sonicated for greater than or equal to 30 s released vacuole-located pigment into the surrounding medium, whereas cells sonicated for < 30 s and control (unsonicated) cells released no pigment. Sonicated cells were viable as determined using fluorescein diacetate; microscopic examination of a selected population of sonicated cells did not show any signs of obvious structural damage. Sonicated cell samples were subcultured to re-establish actively growing, pigment-competent cell cultures. Cells from such cultures were available for further harvests of the pigment by sonication. For sonication periods greater than or equal to 50 s, repeated cycles of sonication and incubation allowed pigments to be harvested from cells every 7 days during a 21-day culture period.</abstract><cop>Berlin</cop><pub>Springer</pub><doi>10.1007/BF00176663</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0175-7598 |
| ispartof | Applied microbiology and biotechnology, 1990-07, Vol.33 (4), p.448-451 |
| issn | 0175-7598 1432-0614 |
| language | eng |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Beta vulgaris Biological and medical sciences Biotechnology cell suspension culture disruption Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology isolation metabolites Methods. Procedures. Technologies Miscellaneous nondestructive methods pigments Plant cells and fungal cells plant pigments suspension culture ultrasonic treatment ultrasonics ultrasound vacuoles |
| title | Repeated harvest of vacuole-located secondary product from in vitro grown plant cells using 1.02 MHz ultrasound |
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