The AdoCbl-riboswitch interaction investigated by in-line probing and surface plasmon resonance spectroscopy (SPR)

The unique feature of riboswitches is to control selected gene expression by specific recognition of a cognate ligand. AdoCbl (adenosyl cobalamin, coenzyme B12) riboswitches regulate the expression of enzymes and transporters involved in bacterial AdoCbl biosynthesis or uptake on a transcriptional a...

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Veröffentlicht in:	Methods in enzymology 2014, Vol.549, p.467-488
Hauptverfasser:	Schaffer, Michelle F, Choudhary, Pallavi K, Sigel, Roland K O
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Outer Membrane Proteins - genetics Base Sequence Binding Sites Cobamides - metabolism Escherichia coli - genetics Escherichia coli - metabolism Escherichia coli Proteins - genetics Membrane Transport Proteins - genetics Molecular Sequence Data Riboswitch RNA, Bacterial - chemistry RNA, Bacterial - genetics RNA, Bacterial - metabolism Surface Plasmon Resonance - methods
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