Chromatographic analysis of some drugs employed in erectile dysfunction therapy: Qualitative and quantitative studies using calixarene stationary phase
In this study, the effect of change in chromatographic process variables on the retention behavior of four drugs employed in erectile dysfunction therapy on a calixarene stationary phase is described. Three of these drugs are known to treat erectile dysfunction, namely, sildenafil citrate, tadalafil...
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| Veröffentlicht in: | Journal of separation science 2014-10, Vol.37 (20), p.2814-2824 |
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| creator | Hashem, Hisham Ibrahim, Adel Ehab Elhenawee, Magda |
| description | In this study, the effect of change in chromatographic process variables on the retention behavior of four drugs employed in erectile dysfunction therapy on a calixarene stationary phase is described. Three of these drugs are known to treat erectile dysfunction, namely, sildenafil citrate, tadalafil, and apomorphine hydrochloride, and one drug that is used as opioid analgesic, tramadol hydrochloride, which is quiet widely misused to treat premature ejaculation. The results indicate the importance of considering the structure and pKa values of drugs to be separated along with mobile phase composition. A new optimized, rapid, and accurate liquid chromatography method is also established for simultaneous determination of sildenafil citrate, tadalafil, and apomorphine hydrochloride in pharmaceutical preparations and bulk powders. The chromatographic separation of the three pharmaceuticals was achieved on a calixarene column in less than 10 min using a binary mobile phase of 35% acetonitrile and 65% 50 mM sodium perchlorate pH2.5 at 1 mL/min flow rate. The method was validated for system efficiency, linearity, accuracy, precision, limits of detection and quantitation, specificity, stability, and robustness. Statistical analysis proved that the method enabled reproducible and selective quantification of all three analytes in bulk drugs and in pharmaceutical preparations. |
| doi_str_mv | 10.1002/jssc.201400276 |
| format | Article |
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Three of these drugs are known to treat erectile dysfunction, namely, sildenafil citrate, tadalafil, and apomorphine hydrochloride, and one drug that is used as opioid analgesic, tramadol hydrochloride, which is quiet widely misused to treat premature ejaculation. The results indicate the importance of considering the structure and pKa values of drugs to be separated along with mobile phase composition. A new optimized, rapid, and accurate liquid chromatography method is also established for simultaneous determination of sildenafil citrate, tadalafil, and apomorphine hydrochloride in pharmaceutical preparations and bulk powders. The chromatographic separation of the three pharmaceuticals was achieved on a calixarene column in less than 10 min using a binary mobile phase of 35% acetonitrile and 65% 50 mM sodium perchlorate pH2.5 at 1 mL/min flow rate. The method was validated for system efficiency, linearity, accuracy, precision, limits of detection and quantitation, specificity, stability, and robustness. Statistical analysis proved that the method enabled reproducible and selective quantification of all three analytes in bulk drugs and in pharmaceutical preparations.</description><identifier>ISSN: 1615-9306</identifier><identifier>EISSN: 1615-9314</identifier><identifier>DOI: 10.1002/jssc.201400276</identifier><identifier>PMID: 25175553</identifier><language>eng</language><publisher>Weinheim: Blackwell Publishing Ltd</publisher><subject>Analysis ; Apomorphine - analysis ; Apomorphine - therapeutic use ; Biological and medical sciences ; Buffers ; Calixarene column ; Calixarenes ; Calixarenes - chemistry ; Carbolines - analysis ; Carbolines - therapeutic use ; Chromatographic variables ; Chromatography ; Chromatography, High Pressure Liquid - methods ; Citrates ; Drugs ; Erectile dysfunction ; Erectile Dysfunction - drug therapy ; General pharmacology ; High-performance liquid chromatography ; Humans ; Hydrochlorides ; Hydrogen-Ion Concentration ; Limit of Detection ; Male ; Medical sciences ; Pharmaceutical Preparations - chemistry ; Pharmaceuticals ; Pharmacology ; Pharmacology. Drug treatments ; Piperazines - analysis ; Piperazines - therapeutic use ; Purines - analysis ; Purines - therapeutic use ; Reproducibility of Results ; Sexual disorders ; Sildenafil Citrate ; Sulfonamides - analysis ; Sulfonamides - therapeutic use ; Tadalafil ; Therapy</subject><ispartof>Journal of separation science, 2014-10, Vol.37 (20), p.2814-2824</ispartof><rights>2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2015 INIST-CNRS</rights><rights>2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><rights>2014 WILEY-VCH Verlag GmbH & Co. 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Sep. Science</addtitle><description>In this study, the effect of change in chromatographic process variables on the retention behavior of four drugs employed in erectile dysfunction therapy on a calixarene stationary phase is described. Three of these drugs are known to treat erectile dysfunction, namely, sildenafil citrate, tadalafil, and apomorphine hydrochloride, and one drug that is used as opioid analgesic, tramadol hydrochloride, which is quiet widely misused to treat premature ejaculation. The results indicate the importance of considering the structure and pKa values of drugs to be separated along with mobile phase composition. A new optimized, rapid, and accurate liquid chromatography method is also established for simultaneous determination of sildenafil citrate, tadalafil, and apomorphine hydrochloride in pharmaceutical preparations and bulk powders. The chromatographic separation of the three pharmaceuticals was achieved on a calixarene column in less than 10 min using a binary mobile phase of 35% acetonitrile and 65% 50 mM sodium perchlorate pH2.5 at 1 mL/min flow rate. The method was validated for system efficiency, linearity, accuracy, precision, limits of detection and quantitation, specificity, stability, and robustness. Statistical analysis proved that the method enabled reproducible and selective quantification of all three analytes in bulk drugs and in pharmaceutical preparations.</description><subject>Analysis</subject><subject>Apomorphine - analysis</subject><subject>Apomorphine - therapeutic use</subject><subject>Biological and medical sciences</subject><subject>Buffers</subject><subject>Calixarene column</subject><subject>Calixarenes</subject><subject>Calixarenes - chemistry</subject><subject>Carbolines - analysis</subject><subject>Carbolines - therapeutic use</subject><subject>Chromatographic variables</subject><subject>Chromatography</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Citrates</subject><subject>Drugs</subject><subject>Erectile dysfunction</subject><subject>Erectile Dysfunction - drug therapy</subject><subject>General pharmacology</subject><subject>High-performance liquid chromatography</subject><subject>Humans</subject><subject>Hydrochlorides</subject><subject>Hydrogen-Ion Concentration</subject><subject>Limit of Detection</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Pharmaceutical Preparations - chemistry</subject><subject>Pharmaceuticals</subject><subject>Pharmacology</subject><subject>Pharmacology. Drug treatments</subject><subject>Piperazines - analysis</subject><subject>Piperazines - therapeutic use</subject><subject>Purines - analysis</subject><subject>Purines - therapeutic use</subject><subject>Reproducibility of Results</subject><subject>Sexual disorders</subject><subject>Sildenafil Citrate</subject><subject>Sulfonamides - analysis</subject><subject>Sulfonamides - therapeutic use</subject><subject>Tadalafil</subject><subject>Therapy</subject><issn>1615-9306</issn><issn>1615-9314</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU2LFDEQhhtR3HX16lECIuylx3x32psM7qgsiqyyx5BJV89k7I_ZVLe7_Uv8u2ac2RG8KARSlXreN1Bvlj1ndMYo5a83iH7GKZOpKfSD7JRppvJSMPnwWFN9kj1B3FDKClPSx9kJV6xQSonT7Od8HfvWDf0quu06eOI610wYkPQ1wb4FUsVxhQTabdNPUJHQEYjgh9Ck0YT12KW678iwhuQwvSFfRteEwQ3hBySzityMrhvuH3AYqwBIRgzdivhE3rkI3W7gdjYuTmS7dghPs0e1axCeHe6z7NvFu6_z9_nl58WH-dvL3CvBZQ5uuax1KQ34StXcMA9V7QUzjFO9VMrJ2siKe8NrACcFN6LiWnMuJfOVM-IsO9_7bmN_MwIOtg3ooWlcB_2IlmleCk05-x-UpZOiKBP68i90048xbfY3RQtmCi4SNdtTPvaIEWq7jaFNK7CM2l26dpeuPaabBC8OtuOyheqI38eZgFcHwGFabh1d5wP-4YwxghUscXLP3aYYp398az9eXc1FWcgky_eygAPcHWUufre6EIWy158WVl-X9EItmJXiF6Muz50</recordid><startdate>201410</startdate><enddate>201410</enddate><creator>Hashem, Hisham</creator><creator>Ibrahim, Adel Ehab</creator><creator>Elhenawee, Magda</creator><general>Blackwell Publishing Ltd</general><general>Wiley</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>201410</creationdate><title>Chromatographic analysis of some drugs employed in erectile dysfunction therapy: Qualitative and quantitative studies using calixarene stationary phase</title><author>Hashem, Hisham ; Ibrahim, Adel Ehab ; Elhenawee, Magda</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5324-eabbf6948ecd5f281cedfc3181206b55a4f84d2c82feea43283d26622441cda83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Analysis</topic><topic>Apomorphine - analysis</topic><topic>Apomorphine - therapeutic use</topic><topic>Biological and medical sciences</topic><topic>Buffers</topic><topic>Calixarene column</topic><topic>Calixarenes</topic><topic>Calixarenes - chemistry</topic><topic>Carbolines - analysis</topic><topic>Carbolines - therapeutic use</topic><topic>Chromatographic variables</topic><topic>Chromatography</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Citrates</topic><topic>Drugs</topic><topic>Erectile dysfunction</topic><topic>Erectile Dysfunction - drug therapy</topic><topic>General pharmacology</topic><topic>High-performance liquid chromatography</topic><topic>Humans</topic><topic>Hydrochlorides</topic><topic>Hydrogen-Ion Concentration</topic><topic>Limit of Detection</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Pharmaceutical Preparations - chemistry</topic><topic>Pharmaceuticals</topic><topic>Pharmacology</topic><topic>Pharmacology. Drug treatments</topic><topic>Piperazines - analysis</topic><topic>Piperazines - therapeutic use</topic><topic>Purines - analysis</topic><topic>Purines - therapeutic use</topic><topic>Reproducibility of Results</topic><topic>Sexual disorders</topic><topic>Sildenafil Citrate</topic><topic>Sulfonamides - analysis</topic><topic>Sulfonamides - therapeutic use</topic><topic>Tadalafil</topic><topic>Therapy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hashem, Hisham</creatorcontrib><creatorcontrib>Ibrahim, Adel Ehab</creatorcontrib><creatorcontrib>Elhenawee, Magda</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of separation science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hashem, Hisham</au><au>Ibrahim, Adel Ehab</au><au>Elhenawee, Magda</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chromatographic analysis of some drugs employed in erectile dysfunction therapy: Qualitative and quantitative studies using calixarene stationary phase</atitle><jtitle>Journal of separation science</jtitle><addtitle>J. Sep. Science</addtitle><date>2014-10</date><risdate>2014</risdate><volume>37</volume><issue>20</issue><spage>2814</spage><epage>2824</epage><pages>2814-2824</pages><issn>1615-9306</issn><eissn>1615-9314</eissn><abstract>In this study, the effect of change in chromatographic process variables on the retention behavior of four drugs employed in erectile dysfunction therapy on a calixarene stationary phase is described. Three of these drugs are known to treat erectile dysfunction, namely, sildenafil citrate, tadalafil, and apomorphine hydrochloride, and one drug that is used as opioid analgesic, tramadol hydrochloride, which is quiet widely misused to treat premature ejaculation. The results indicate the importance of considering the structure and pKa values of drugs to be separated along with mobile phase composition. A new optimized, rapid, and accurate liquid chromatography method is also established for simultaneous determination of sildenafil citrate, tadalafil, and apomorphine hydrochloride in pharmaceutical preparations and bulk powders. The chromatographic separation of the three pharmaceuticals was achieved on a calixarene column in less than 10 min using a binary mobile phase of 35% acetonitrile and 65% 50 mM sodium perchlorate pH2.5 at 1 mL/min flow rate. The method was validated for system efficiency, linearity, accuracy, precision, limits of detection and quantitation, specificity, stability, and robustness. Statistical analysis proved that the method enabled reproducible and selective quantification of all three analytes in bulk drugs and in pharmaceutical preparations.</abstract><cop>Weinheim</cop><pub>Blackwell Publishing Ltd</pub><pmid>25175553</pmid><doi>10.1002/jssc.201400276</doi><tpages>11</tpages></addata></record> |
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| subjects | Analysis Apomorphine - analysis Apomorphine - therapeutic use Biological and medical sciences Buffers Calixarene column Calixarenes Calixarenes - chemistry Carbolines - analysis Carbolines - therapeutic use Chromatographic variables Chromatography Chromatography, High Pressure Liquid - methods Citrates Drugs Erectile dysfunction Erectile Dysfunction - drug therapy General pharmacology High-performance liquid chromatography Humans Hydrochlorides Hydrogen-Ion Concentration Limit of Detection Male Medical sciences Pharmaceutical Preparations - chemistry Pharmaceuticals Pharmacology Pharmacology. Drug treatments Piperazines - analysis Piperazines - therapeutic use Purines - analysis Purines - therapeutic use Reproducibility of Results Sexual disorders Sildenafil Citrate Sulfonamides - analysis Sulfonamides - therapeutic use Tadalafil Therapy |
| title | Chromatographic analysis of some drugs employed in erectile dysfunction therapy: Qualitative and quantitative studies using calixarene stationary phase |
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