Heterodimeric complex formation with CD8 and TCR by bispecific antibody sustains paracrine IL-2-dependent growth of CD3 super(+) CD8 super(+) T cells
During physiologic activation of mature CD8 super(+) T cells, TCR and CD8 bind to the same Ag-complexed MHC class I molecule. Thereby, close proximity is induced between CD8 and the TCR/CD3 complex. During this engagement, CD8 may deliver TCR-independent signals via its associated protein tyrosine k...
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| Veröffentlicht in: | The Journal of immunology (1950) 1992-01, Vol.149 (6), p.1840-1846 |
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| container_end_page | 1846 |
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| container_issue | 6 |
| container_start_page | 1840 |
| container_title | The Journal of immunology (1950) |
| container_volume | 149 |
| creator | De Lau, WBM Boom, SE Heije, K Griffioen, A W Braakman, E Bolhuis, RLH Tax, WJM Clevers, H Bast, BJEG |
| description | During physiologic activation of mature CD8 super(+) T cells, TCR and CD8 bind to the same Ag-complexed MHC class I molecule. Thereby, close proximity is induced between CD8 and the TCR/CD3 complex. During this engagement, CD8 may deliver TCR-independent signals via its associated protein tyrosine kinase, p56 super(lck). We studied the potential biologic effects of close association between CD8 and TCR/CD3 complexes by using a bispecific antibody (bsAb) directed against both TCR and CD8 molecules. The data suggest that bsAb-induced activation differs from activation by monospecific anti-TCR antibody. The former appears to more closely mimic physiologic Ag-induced signaling, because it leads to a similar paracrine IL-2-dependent growth pattern. The bsAb may, therefore, be instrumental in studying T cell signaling pathways, in particular the role of CD8-associated p56 super(lck) therein. |
| format | Article |
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Thereby, close proximity is induced between CD8 and the TCR/CD3 complex. During this engagement, CD8 may deliver TCR-independent signals via its associated protein tyrosine kinase, p56 super(lck). We studied the potential biologic effects of close association between CD8 and TCR/CD3 complexes by using a bispecific antibody (bsAb) directed against both TCR and CD8 molecules. The data suggest that bsAb-induced activation differs from activation by monospecific anti-TCR antibody. The former appears to more closely mimic physiologic Ag-induced signaling, because it leads to a similar paracrine IL-2-dependent growth pattern. The bsAb may, therefore, be instrumental in studying T cell signaling pathways, in particular the role of CD8-associated p56 super(lck) therein.</abstract></addata></record> |
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| identifier | ISSN: 0022-1767 |
| ispartof | The Journal of immunology (1950), 1992-01, Vol.149 (6), p.1840-1846 |
| issn | 0022-1767 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16292945 |
| source | Alma/SFX Local Collection |
| title | Heterodimeric complex formation with CD8 and TCR by bispecific antibody sustains paracrine IL-2-dependent growth of CD3 super(+) CD8 super(+) T cells |
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