5' end maturation and RNA editing have to precede tRNA 3' processing in plant mitochondria
We report the characterization and partial purification of potato mitochondrial RNase Z, an endonuclease that generates mature tRNA 3' ends. The enzyme consists of one (or more) protein(s) without RNA subunits. Products of the processing reaction are tRNA molecules with 3' terminal hydroxy...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1998-01, Vol.95 (1), p.108-113 |
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| creator | Kunzmann, A Brennicke, A Marchfelder, A |
| description | We report the characterization and partial purification of potato mitochondrial RNase Z, an endonuclease that generates mature tRNA 3' ends. The enzyme consists of one (or more) protein(s) without RNA subunits. Products of the processing reaction are tRNA molecules with 3' terminal hydroxyl groups and 3' trailers with 5' terminal phosphates. The main processing sites are located immediately 3' to the discriminator and one nucleotide further downstream. This endonucleolytic processing at and dose to the tRNA 3' end in potato mitochondria suggests a higher similarity to the eukaryotic than to the prokaryotic tRNA 3' processing pathway. Partial purification and separation of RNase Z from the 5' processing activity RNase P allowed us to determine biochemical characteristics of the enzyme. The activity is stable over broad pH and temperature ranges, with peak activity at pH 8 and 30 degrees C. Optimal concentrations for MgCl2 and KCl are 5 mM and 30 mM, respectively. The potato mitochondrial RNase Z accepts only tRNA precursors with mature 5' ends. The precursor for tRNA Phe requires RNA editing for efficient processing by RNase Z |
| doi_str_mv | 10.1073/pnas.95.1.108 |
| format | Article |
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The enzyme consists of one (or more) protein(s) without RNA subunits. Products of the processing reaction are tRNA molecules with 3' terminal hydroxyl groups and 3' trailers with 5' terminal phosphates. The main processing sites are located immediately 3' to the discriminator and one nucleotide further downstream. This endonucleolytic processing at and dose to the tRNA 3' end in potato mitochondria suggests a higher similarity to the eukaryotic than to the prokaryotic tRNA 3' processing pathway. Partial purification and separation of RNase Z from the 5' processing activity RNase P allowed us to determine biochemical characteristics of the enzyme. The activity is stable over broad pH and temperature ranges, with peak activity at pH 8 and 30 degrees C. Optimal concentrations for MgCl2 and KCl are 5 mM and 30 mM, respectively. The potato mitochondrial RNase Z accepts only tRNA precursors with mature 5' ends. The precursor for tRNA Phe requires RNA editing for efficient processing by RNase Z</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.95.1.108</identifier><identifier>PMID: 9419337</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>ACTIVIDAD ENZIMATICA ; ACTIVITE ENZYMATIQUE ; ARN ; ARN DE TRANSFERENCIA ; ARN DE TRANSFERT ; Base Sequence ; Biochemistry ; Biological Sciences ; CHEMICAL REACTIONS ; ENDONUCLEOLYTIC PROCESSING ; Endoribonucleases - isolation & purification ; Endoribonucleases - metabolism ; Enzymes ; ENZYMIC ACTIVITY ; Gels ; Hydrogen-Ion Concentration ; MITOCHONDRIA ; Mitochondria - genetics ; MITOCHONDRIE ; MITOCONDRIA ; Molecular Sequence Data ; Molecules ; Nucleic Acid Conformation ; Nucleotides ; Potatoes ; PRECURSORS ; PURIFICACION ; PURIFICATION ; REACCIONES QUIMICAS ; REACTION CHIMIQUE ; RIBONUCLEASAS ; RIBONUCLEASE ; RIBONUCLEASE Z ; RIBONUCLEASES ; Ribonucleic acid ; RNA ; RNA Editing ; RNA precursors ; RNA Precursors - metabolism ; RNA, Plant - metabolism ; SOLANUM TUBEROSUM ; Solanum tuberosum - enzymology ; Solanum tuberosum - genetics ; Temperature ; TIROSINA ; Trailers ; TRANSFER RNA ; TYROSINE</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1998-01, Vol.95 (1), p.108-113</ispartof><rights>Copyright 1993-1998 National Academy of Sciences</rights><rights>Copyright National Academy of Sciences Jan 6, 1998</rights><rights>Copyright © 1998, The National Academy of Sciences 1998</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c596t-f760e9f7bd84b8c847be805bc8d4833f8a5dd1af079f0b925d469cd9c18d13823</citedby><cites>FETCH-LOGICAL-c596t-f760e9f7bd84b8c847be805bc8d4833f8a5dd1af079f0b925d469cd9c18d13823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/95/1.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/44424$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/44424$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,724,777,781,800,882,27905,27906,53772,53774,57998,58231</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9419337$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kunzmann, A</creatorcontrib><creatorcontrib>Brennicke, A</creatorcontrib><creatorcontrib>Marchfelder, A</creatorcontrib><title>5' end maturation and RNA editing have to precede tRNA 3' processing in plant mitochondria</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>We report the characterization and partial purification of potato mitochondrial RNase Z, an endonuclease that generates mature tRNA 3' ends. The enzyme consists of one (or more) protein(s) without RNA subunits. Products of the processing reaction are tRNA molecules with 3' terminal hydroxyl groups and 3' trailers with 5' terminal phosphates. The main processing sites are located immediately 3' to the discriminator and one nucleotide further downstream. This endonucleolytic processing at and dose to the tRNA 3' end in potato mitochondria suggests a higher similarity to the eukaryotic than to the prokaryotic tRNA 3' processing pathway. Partial purification and separation of RNase Z from the 5' processing activity RNase P allowed us to determine biochemical characteristics of the enzyme. The activity is stable over broad pH and temperature ranges, with peak activity at pH 8 and 30 degrees C. Optimal concentrations for MgCl2 and KCl are 5 mM and 30 mM, respectively. The potato mitochondrial RNase Z accepts only tRNA precursors with mature 5' ends. The precursor for tRNA Phe requires RNA editing for efficient processing by RNase Z</description><subject>ACTIVIDAD ENZIMATICA</subject><subject>ACTIVITE ENZYMATIQUE</subject><subject>ARN</subject><subject>ARN DE TRANSFERENCIA</subject><subject>ARN DE TRANSFERT</subject><subject>Base Sequence</subject><subject>Biochemistry</subject><subject>Biological Sciences</subject><subject>CHEMICAL REACTIONS</subject><subject>ENDONUCLEOLYTIC PROCESSING</subject><subject>Endoribonucleases - isolation & purification</subject><subject>Endoribonucleases - metabolism</subject><subject>Enzymes</subject><subject>ENZYMIC ACTIVITY</subject><subject>Gels</subject><subject>Hydrogen-Ion Concentration</subject><subject>MITOCHONDRIA</subject><subject>Mitochondria - genetics</subject><subject>MITOCHONDRIE</subject><subject>MITOCONDRIA</subject><subject>Molecular Sequence Data</subject><subject>Molecules</subject><subject>Nucleic Acid Conformation</subject><subject>Nucleotides</subject><subject>Potatoes</subject><subject>PRECURSORS</subject><subject>PURIFICACION</subject><subject>PURIFICATION</subject><subject>REACCIONES QUIMICAS</subject><subject>REACTION CHIMIQUE</subject><subject>RIBONUCLEASAS</subject><subject>RIBONUCLEASE</subject><subject>RIBONUCLEASE Z</subject><subject>RIBONUCLEASES</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA Editing</subject><subject>RNA precursors</subject><subject>RNA Precursors - metabolism</subject><subject>RNA, Plant - metabolism</subject><subject>SOLANUM TUBEROSUM</subject><subject>Solanum tuberosum - enzymology</subject><subject>Solanum tuberosum - genetics</subject><subject>Temperature</subject><subject>TIROSINA</subject><subject>Trailers</subject><subject>TRANSFER RNA</subject><subject>TYROSINE</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks1vFCEYh4nR1LV69GI0mZjUnmaFAQZIvDRN_UgaTdRevBAGmF02szAFptH_XiY7WT8OeoI3z_MjvLwA8BTBNYIMvx69SmtB16iU_B5YIShQ3RIB74MVhA2rOWnIQ_AopR2EUFAOT8CJIEhgzFbgGz2vrDfVXuUpquyCr1QpP3-8qKxx2flNtVV3tsqhGqPV1pTtDPF5qYO2Kc2K89U4KJ-rvctBb4M30anH4EGvhmSfLOspuHl79fXyfX396d2Hy4vrWlPR5rpnLbSiZ53hpOOaE9ZZDmmnuSEc454ragxSPWSih51oqCGt0EZoxA3CvMGn4M3h3HHq9tZo63NUgxyj26v4Qwbl5J_Eu63chDuJOCJz_NUSj-F2sinLvUvaDqUfG6YkmWgb3qD_i6htBKLNLL78S9yFKfryBrKBCIvSCSlSfZB0DClF2x8vjKCcByvnwUpBJSolL_6L37s82sskCz9b-Bw70iUu-2kYsv2ei_f8H17Bzw54l3KIR05I-US_7tCrINUmuiRvviAhGGSQMo5_Am99xzU</recordid><startdate>19980106</startdate><enddate>19980106</enddate><creator>Kunzmann, A</creator><creator>Brennicke, A</creator><creator>Marchfelder, A</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19980106</creationdate><title>5' end maturation and RNA editing have to precede tRNA 3' processing in plant mitochondria</title><author>Kunzmann, A ; Brennicke, A ; Marchfelder, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c596t-f760e9f7bd84b8c847be805bc8d4833f8a5dd1af079f0b925d469cd9c18d13823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>ACTIVIDAD ENZIMATICA</topic><topic>ACTIVITE ENZYMATIQUE</topic><topic>ARN</topic><topic>ARN DE TRANSFERENCIA</topic><topic>ARN DE TRANSFERT</topic><topic>Base Sequence</topic><topic>Biochemistry</topic><topic>Biological Sciences</topic><topic>CHEMICAL REACTIONS</topic><topic>ENDONUCLEOLYTIC PROCESSING</topic><topic>Endoribonucleases - isolation & purification</topic><topic>Endoribonucleases - metabolism</topic><topic>Enzymes</topic><topic>ENZYMIC ACTIVITY</topic><topic>Gels</topic><topic>Hydrogen-Ion Concentration</topic><topic>MITOCHONDRIA</topic><topic>Mitochondria - genetics</topic><topic>MITOCHONDRIE</topic><topic>MITOCONDRIA</topic><topic>Molecular Sequence Data</topic><topic>Molecules</topic><topic>Nucleic Acid Conformation</topic><topic>Nucleotides</topic><topic>Potatoes</topic><topic>PRECURSORS</topic><topic>PURIFICACION</topic><topic>PURIFICATION</topic><topic>REACCIONES QUIMICAS</topic><topic>REACTION CHIMIQUE</topic><topic>RIBONUCLEASAS</topic><topic>RIBONUCLEASE</topic><topic>RIBONUCLEASE Z</topic><topic>RIBONUCLEASES</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA Editing</topic><topic>RNA precursors</topic><topic>RNA Precursors - metabolism</topic><topic>RNA, Plant - metabolism</topic><topic>SOLANUM TUBEROSUM</topic><topic>Solanum tuberosum - enzymology</topic><topic>Solanum tuberosum - genetics</topic><topic>Temperature</topic><topic>TIROSINA</topic><topic>Trailers</topic><topic>TRANSFER RNA</topic><topic>TYROSINE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kunzmann, A</creatorcontrib><creatorcontrib>Brennicke, A</creatorcontrib><creatorcontrib>Marchfelder, A</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kunzmann, A</au><au>Brennicke, A</au><au>Marchfelder, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>5' end maturation and RNA editing have to precede tRNA 3' processing in plant mitochondria</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1998-01-06</date><risdate>1998</risdate><volume>95</volume><issue>1</issue><spage>108</spage><epage>113</epage><pages>108-113</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>We report the characterization and partial purification of potato mitochondrial RNase Z, an endonuclease that generates mature tRNA 3' ends. The enzyme consists of one (or more) protein(s) without RNA subunits. Products of the processing reaction are tRNA molecules with 3' terminal hydroxyl groups and 3' trailers with 5' terminal phosphates. The main processing sites are located immediately 3' to the discriminator and one nucleotide further downstream. This endonucleolytic processing at and dose to the tRNA 3' end in potato mitochondria suggests a higher similarity to the eukaryotic than to the prokaryotic tRNA 3' processing pathway. Partial purification and separation of RNase Z from the 5' processing activity RNase P allowed us to determine biochemical characteristics of the enzyme. The activity is stable over broad pH and temperature ranges, with peak activity at pH 8 and 30 degrees C. Optimal concentrations for MgCl2 and KCl are 5 mM and 30 mM, respectively. The potato mitochondrial RNase Z accepts only tRNA precursors with mature 5' ends. The precursor for tRNA Phe requires RNA editing for efficient processing by RNase Z</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>9419337</pmid><doi>10.1073/pnas.95.1.108</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| subjects | ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE ARN ARN DE TRANSFERENCIA ARN DE TRANSFERT Base Sequence Biochemistry Biological Sciences CHEMICAL REACTIONS ENDONUCLEOLYTIC PROCESSING Endoribonucleases - isolation & purification Endoribonucleases - metabolism Enzymes ENZYMIC ACTIVITY Gels Hydrogen-Ion Concentration MITOCHONDRIA Mitochondria - genetics MITOCHONDRIE MITOCONDRIA Molecular Sequence Data Molecules Nucleic Acid Conformation Nucleotides Potatoes PRECURSORS PURIFICACION PURIFICATION REACCIONES QUIMICAS REACTION CHIMIQUE RIBONUCLEASAS RIBONUCLEASE RIBONUCLEASE Z RIBONUCLEASES Ribonucleic acid RNA RNA Editing RNA precursors RNA Precursors - metabolism RNA, Plant - metabolism SOLANUM TUBEROSUM Solanum tuberosum - enzymology Solanum tuberosum - genetics Temperature TIROSINA Trailers TRANSFER RNA TYROSINE |
| title | 5' end maturation and RNA editing have to precede tRNA 3' processing in plant mitochondria |
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