The effects of cadmium exposure on the cytology and function of primary cultures from rainbow trout

Cultured epidermal cells from explants of skin of rainbow trout were used to study the cytological and functional changes following sublethal exposure to cadmium stress. The aim was to develop diagnostic markers for ecotoxicology. Cultures were exposed to the pollutant for 48 h. Cell structural and...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Cell biochemistry and function 1998-03, Vol.16 (1), p.1-13
Hauptverfasser: Lyons-Alcantara, Maria, Mooney, Robert, Lyng, Fiona, Cottell, David, Mothersill, Carmel
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 13
container_issue 1
container_start_page 1
container_title Cell biochemistry and function
container_volume 16
creator Lyons-Alcantara, Maria
Mooney, Robert
Lyng, Fiona
Cottell, David
Mothersill, Carmel
description Cultured epidermal cells from explants of skin of rainbow trout were used to study the cytological and functional changes following sublethal exposure to cadmium stress. The aim was to develop diagnostic markers for ecotoxicology. Cultures were exposed to the pollutant for 48 h. Cell structural and cytological changes were established by light and electron microscopy. Metabolic alterations were detected by immunohistochemistry. The relation between the initiation of cellular alterations and cadmium concentrations was compared in cultures exposed in commercially‐available serum‐free and serum‐containing medium. The expression of stress proteins (metallothionein and heat shock protein) was also studied. Rainbow trout epithelial cells exposed to cadmium showed typical morphological changes indicative of cell death by apoptosis. Sublethal exposure also resulted in cellular metabolic disturbances with increased deposits of glycogen. Increased melanization was also observed. These changes appeared at lower concentrations of cadmium when cells were exposed in serum‐free media than in serum‐containing media. Cadmium induced the expression of heat shock proteins but not of metallothioneins. The results broadly confirm in vivo findings for cadmium toxicity and suggest that this in vitro technique may have applications in aquatic toxicology. © 1998 John Wiley & Sons, Ltd.
doi_str_mv 10.1002/(SICI)1099-0844(199803)16:1<1::AID-CBF754>3.0.CO;2-W
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_16285645</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>16285645</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4314-daa5eb7c2aea042d253c0cc8fd22910f6f30f77b46329db953e2854b174485183</originalsourceid><addsrcrecordid>eNp9kF1v0zAUhiMEGmXwE5B8hbaLFH_FicuEtIVtVJqoEGW9PHIcGwJJXOxEW__9XKXqDQj5wpLPe55XfpLkguA5wZi-P_u2LJfnBEuZ4oLzMyJlgdk5EQtyQRaLy-WntLy6yTP-kc3xvFx9oOnmWTI7LjxPZpgKlgpe8JfJqxB-YYylYPgkOZEZkTwTs0SvfxpkrDV6CMhZpFXdNWOHzOPWhdEb5Ho0xIjeDa51P3ZI9TWyY6-HJk7iwtY3nfI7pMd2iPmArHcd8qrpK_eABu_G4XXywqo2mDeH-zT5fnO9Lj-nd6vbZXl5l2rOCE9rpTJT5ZoqozCnNc2YxloXtqZUEmyFZdjmecUFo7KuZMYMLTJekZzzIiMFO03eTdytd39GEwbomqBN26reuDEAETEveBaD6ymovQvBGwuHXwDBsHcPsHcPe5WwVwmT-0gAEg9E9zC5BwYYyhVQ2ETs20P_WHWmPkIPsuP8fpo_NK3Z_dX538p_Nh5eIjidwE0YzOMRrPxvEDnLM9h8uYWrSCNi_RXu2RNPMa6P</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16285645</pqid></control><display><type>article</type><title>The effects of cadmium exposure on the cytology and function of primary cultures from rainbow trout</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Lyons-Alcantara, Maria ; Mooney, Robert ; Lyng, Fiona ; Cottell, David ; Mothersill, Carmel</creator><creatorcontrib>Lyons-Alcantara, Maria ; Mooney, Robert ; Lyng, Fiona ; Cottell, David ; Mothersill, Carmel</creatorcontrib><description>Cultured epidermal cells from explants of skin of rainbow trout were used to study the cytological and functional changes following sublethal exposure to cadmium stress. The aim was to develop diagnostic markers for ecotoxicology. Cultures were exposed to the pollutant for 48 h. Cell structural and cytological changes were established by light and electron microscopy. Metabolic alterations were detected by immunohistochemistry. The relation between the initiation of cellular alterations and cadmium concentrations was compared in cultures exposed in commercially‐available serum‐free and serum‐containing medium. The expression of stress proteins (metallothionein and heat shock protein) was also studied. Rainbow trout epithelial cells exposed to cadmium showed typical morphological changes indicative of cell death by apoptosis. Sublethal exposure also resulted in cellular metabolic disturbances with increased deposits of glycogen. Increased melanization was also observed. These changes appeared at lower concentrations of cadmium when cells were exposed in serum‐free media than in serum‐containing media. Cadmium induced the expression of heat shock proteins but not of metallothioneins. The results broadly confirm in vivo findings for cadmium toxicity and suggest that this in vitro technique may have applications in aquatic toxicology. © 1998 John Wiley &amp; Sons, Ltd.</description><identifier>ISSN: 0263-6484</identifier><identifier>EISSN: 1099-0844</identifier><identifier>DOI: 10.1002/(SICI)1099-0844(199803)16:1&lt;1::AID-CBF754&gt;3.0.CO;2-W</identifier><identifier>PMID: 9519456</identifier><language>eng</language><publisher>Chichester, UK: John Wiley &amp; Sons, Ltd</publisher><subject>Animals ; Antioxidants - pharmacology ; Apoptosis ; cadmium ; Cadmium - pharmacology ; Cadmium - toxicity ; Cell Differentiation - drug effects ; Cells, Cultured ; cytotoxicity ; epithelial cells ; Epithelial Cells - drug effects ; Epithelial Cells - ultrastructure ; explants ; Freshwater ; Immunoenzyme Techniques ; Marine ; Microscopy, Electron ; Oncorhynchus mykiss ; rainbow trout ; serum-free medium ; Skin - drug effects</subject><ispartof>Cell biochemistry and function, 1998-03, Vol.16 (1), p.1-13</ispartof><rights>Copyright © 1998 John Wiley &amp; Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c4314-daa5eb7c2aea042d253c0cc8fd22910f6f30f77b46329db953e2854b174485183</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F%28SICI%291099-0844%28199803%2916%3A1%3C1%3A%3AAID-CBF754%3E3.0.CO%3B2-W$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F%28SICI%291099-0844%28199803%2916%3A1%3C1%3A%3AAID-CBF754%3E3.0.CO%3B2-W$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9519456$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lyons-Alcantara, Maria</creatorcontrib><creatorcontrib>Mooney, Robert</creatorcontrib><creatorcontrib>Lyng, Fiona</creatorcontrib><creatorcontrib>Cottell, David</creatorcontrib><creatorcontrib>Mothersill, Carmel</creatorcontrib><title>The effects of cadmium exposure on the cytology and function of primary cultures from rainbow trout</title><title>Cell biochemistry and function</title><addtitle>Cell Biochem. Funct</addtitle><description>Cultured epidermal cells from explants of skin of rainbow trout were used to study the cytological and functional changes following sublethal exposure to cadmium stress. The aim was to develop diagnostic markers for ecotoxicology. Cultures were exposed to the pollutant for 48 h. Cell structural and cytological changes were established by light and electron microscopy. Metabolic alterations were detected by immunohistochemistry. The relation between the initiation of cellular alterations and cadmium concentrations was compared in cultures exposed in commercially‐available serum‐free and serum‐containing medium. The expression of stress proteins (metallothionein and heat shock protein) was also studied. Rainbow trout epithelial cells exposed to cadmium showed typical morphological changes indicative of cell death by apoptosis. Sublethal exposure also resulted in cellular metabolic disturbances with increased deposits of glycogen. Increased melanization was also observed. These changes appeared at lower concentrations of cadmium when cells were exposed in serum‐free media than in serum‐containing media. Cadmium induced the expression of heat shock proteins but not of metallothioneins. The results broadly confirm in vivo findings for cadmium toxicity and suggest that this in vitro technique may have applications in aquatic toxicology. © 1998 John Wiley &amp; Sons, Ltd.</description><subject>Animals</subject><subject>Antioxidants - pharmacology</subject><subject>Apoptosis</subject><subject>cadmium</subject><subject>Cadmium - pharmacology</subject><subject>Cadmium - toxicity</subject><subject>Cell Differentiation - drug effects</subject><subject>Cells, Cultured</subject><subject>cytotoxicity</subject><subject>epithelial cells</subject><subject>Epithelial Cells - drug effects</subject><subject>Epithelial Cells - ultrastructure</subject><subject>explants</subject><subject>Freshwater</subject><subject>Immunoenzyme Techniques</subject><subject>Marine</subject><subject>Microscopy, Electron</subject><subject>Oncorhynchus mykiss</subject><subject>rainbow trout</subject><subject>serum-free medium</subject><subject>Skin - drug effects</subject><issn>0263-6484</issn><issn>1099-0844</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kF1v0zAUhiMEGmXwE5B8hbaLFH_FicuEtIVtVJqoEGW9PHIcGwJJXOxEW__9XKXqDQj5wpLPe55XfpLkguA5wZi-P_u2LJfnBEuZ4oLzMyJlgdk5EQtyQRaLy-WntLy6yTP-kc3xvFx9oOnmWTI7LjxPZpgKlgpe8JfJqxB-YYylYPgkOZEZkTwTs0SvfxpkrDV6CMhZpFXdNWOHzOPWhdEb5Ho0xIjeDa51P3ZI9TWyY6-HJk7iwtY3nfI7pMd2iPmArHcd8qrpK_eABu_G4XXywqo2mDeH-zT5fnO9Lj-nd6vbZXl5l2rOCE9rpTJT5ZoqozCnNc2YxloXtqZUEmyFZdjmecUFo7KuZMYMLTJekZzzIiMFO03eTdytd39GEwbomqBN26reuDEAETEveBaD6ymovQvBGwuHXwDBsHcPsHcPe5WwVwmT-0gAEg9E9zC5BwYYyhVQ2ETs20P_WHWmPkIPsuP8fpo_NK3Z_dX538p_Nh5eIjidwE0YzOMRrPxvEDnLM9h8uYWrSCNi_RXu2RNPMa6P</recordid><startdate>199803</startdate><enddate>199803</enddate><creator>Lyons-Alcantara, Maria</creator><creator>Mooney, Robert</creator><creator>Lyng, Fiona</creator><creator>Cottell, David</creator><creator>Mothersill, Carmel</creator><general>John Wiley &amp; Sons, Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H97</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope></search><sort><creationdate>199803</creationdate><title>The effects of cadmium exposure on the cytology and function of primary cultures from rainbow trout</title><author>Lyons-Alcantara, Maria ; Mooney, Robert ; Lyng, Fiona ; Cottell, David ; Mothersill, Carmel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4314-daa5eb7c2aea042d253c0cc8fd22910f6f30f77b46329db953e2854b174485183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Antioxidants - pharmacology</topic><topic>Apoptosis</topic><topic>cadmium</topic><topic>Cadmium - pharmacology</topic><topic>Cadmium - toxicity</topic><topic>Cell Differentiation - drug effects</topic><topic>Cells, Cultured</topic><topic>cytotoxicity</topic><topic>epithelial cells</topic><topic>Epithelial Cells - drug effects</topic><topic>Epithelial Cells - ultrastructure</topic><topic>explants</topic><topic>Freshwater</topic><topic>Immunoenzyme Techniques</topic><topic>Marine</topic><topic>Microscopy, Electron</topic><topic>Oncorhynchus mykiss</topic><topic>rainbow trout</topic><topic>serum-free medium</topic><topic>Skin - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lyons-Alcantara, Maria</creatorcontrib><creatorcontrib>Mooney, Robert</creatorcontrib><creatorcontrib>Lyng, Fiona</creatorcontrib><creatorcontrib>Cottell, David</creatorcontrib><creatorcontrib>Mothersill, Carmel</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) 3: Aquatic Pollution &amp; Environmental Quality</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Cell biochemistry and function</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lyons-Alcantara, Maria</au><au>Mooney, Robert</au><au>Lyng, Fiona</au><au>Cottell, David</au><au>Mothersill, Carmel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effects of cadmium exposure on the cytology and function of primary cultures from rainbow trout</atitle><jtitle>Cell biochemistry and function</jtitle><addtitle>Cell Biochem. Funct</addtitle><date>1998-03</date><risdate>1998</risdate><volume>16</volume><issue>1</issue><spage>1</spage><epage>13</epage><pages>1-13</pages><issn>0263-6484</issn><eissn>1099-0844</eissn><abstract>Cultured epidermal cells from explants of skin of rainbow trout were used to study the cytological and functional changes following sublethal exposure to cadmium stress. The aim was to develop diagnostic markers for ecotoxicology. Cultures were exposed to the pollutant for 48 h. Cell structural and cytological changes were established by light and electron microscopy. Metabolic alterations were detected by immunohistochemistry. The relation between the initiation of cellular alterations and cadmium concentrations was compared in cultures exposed in commercially‐available serum‐free and serum‐containing medium. The expression of stress proteins (metallothionein and heat shock protein) was also studied. Rainbow trout epithelial cells exposed to cadmium showed typical morphological changes indicative of cell death by apoptosis. Sublethal exposure also resulted in cellular metabolic disturbances with increased deposits of glycogen. Increased melanization was also observed. These changes appeared at lower concentrations of cadmium when cells were exposed in serum‐free media than in serum‐containing media. Cadmium induced the expression of heat shock proteins but not of metallothioneins. The results broadly confirm in vivo findings for cadmium toxicity and suggest that this in vitro technique may have applications in aquatic toxicology. © 1998 John Wiley &amp; Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley &amp; Sons, Ltd</pub><pmid>9519456</pmid><doi>10.1002/(SICI)1099-0844(199803)16:1&lt;1::AID-CBF754&gt;3.0.CO;2-W</doi><tpages>13</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0263-6484
ispartof Cell biochemistry and function, 1998-03, Vol.16 (1), p.1-13
issn 0263-6484
1099-0844
language eng
recordid cdi_proquest_miscellaneous_16285645
source MEDLINE; Access via Wiley Online Library
subjects Animals
Antioxidants - pharmacology
Apoptosis
cadmium
Cadmium - pharmacology
Cadmium - toxicity
Cell Differentiation - drug effects
Cells, Cultured
cytotoxicity
epithelial cells
Epithelial Cells - drug effects
Epithelial Cells - ultrastructure
explants
Freshwater
Immunoenzyme Techniques
Marine
Microscopy, Electron
Oncorhynchus mykiss
rainbow trout
serum-free medium
Skin - drug effects
title The effects of cadmium exposure on the cytology and function of primary cultures from rainbow trout
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T16%3A19%3A50IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20effects%20of%20cadmium%20exposure%20on%20the%20cytology%20and%20function%20of%20primary%20cultures%20from%20rainbow%20trout&rft.jtitle=Cell%20biochemistry%20and%20function&rft.au=Lyons-Alcantara,%20Maria&rft.date=1998-03&rft.volume=16&rft.issue=1&rft.spage=1&rft.epage=13&rft.pages=1-13&rft.issn=0263-6484&rft.eissn=1099-0844&rft_id=info:doi/10.1002/(SICI)1099-0844(199803)16:1%3C1::AID-CBF754%3E3.0.CO;2-W&rft_dat=%3Cproquest_cross%3E16285645%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16285645&rft_id=info:pmid/9519456&rfr_iscdi=true