Fucoxanthin induces apoptosis in human cervical cancer cell line HeLa via PI3K/Akt pathway
Cervical cancer (CC) is a malignant neoplasm arising from cells originating in the cervix uteri, among the top causes of death from cancer in women. In a gene expression profiling study of metabolic response to treatment, PI3K/Akt signaling pathway are associated with the development of CC. A common...
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| Veröffentlicht in: | Tumor biology 2014-11, Vol.35 (11), p.11261-11267 |
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| creator | Ye, Guoliu Lu, Qin Zhao, Weidong Du, Danli Jin, Lijie Liu, Yusheng |
| description | Cervical cancer (CC) is a malignant neoplasm arising from cells originating in the cervix uteri, among the top causes of death from cancer in women. In a gene expression profiling study of metabolic response to treatment, PI3K/Akt signaling pathway are associated with the development of CC. A common mechanism of Akt activation seen in cancer types is alterations in the upstream regulators of Akt such as phosphatidylinositol 3-kinase (PI3K), which is overexpressed in cervical cancer tissues, and leads to phosphorylation of Akt. Both PI3K and Akt inhibitors exist and may be therapeutically valuable. In the present study, we use MTT assay and western blot for the high-throughput screening to select specific inhibitors of PI3K/Akt signaling pathway, and then obtain fucoxanthin. Fucoxanthin is a water-soluble dietary fiber, taken from the unique slimy component of alginic cells. Various studies have pointed out that fucoxanthin is very effective for the treatment of cancer. Our results have shown that fucoxanthin induced a significant apoptosis of HeLa cells, compared with other candidates. After treatment with fucoxanthin for 24 h, the level of phosphorylation was inhibited in a dose-dependent manner, and the proteins of apoptotic markers were changed in HeLa cells. And fucoxanthin could suppress tumor growth in vivo. In addition, the mitochondrial signal transduction pathway maybe was involved in its mechanism and NF-κB activation was decreased after treatment with fucoxanthin. Therefore, fucoxanthin may be used as anti-cervical cancer drugs in the future. |
| doi_str_mv | 10.1007/s13277-014-2337-7 |
| format | Article |
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In a gene expression profiling study of metabolic response to treatment, PI3K/Akt signaling pathway are associated with the development of CC. A common mechanism of Akt activation seen in cancer types is alterations in the upstream regulators of Akt such as phosphatidylinositol 3-kinase (PI3K), which is overexpressed in cervical cancer tissues, and leads to phosphorylation of Akt. Both PI3K and Akt inhibitors exist and may be therapeutically valuable. In the present study, we use MTT assay and western blot for the high-throughput screening to select specific inhibitors of PI3K/Akt signaling pathway, and then obtain fucoxanthin. Fucoxanthin is a water-soluble dietary fiber, taken from the unique slimy component of alginic cells. Various studies have pointed out that fucoxanthin is very effective for the treatment of cancer. Our results have shown that fucoxanthin induced a significant apoptosis of HeLa cells, compared with other candidates. After treatment with fucoxanthin for 24 h, the level of phosphorylation was inhibited in a dose-dependent manner, and the proteins of apoptotic markers were changed in HeLa cells. And fucoxanthin could suppress tumor growth in vivo. In addition, the mitochondrial signal transduction pathway maybe was involved in its mechanism and NF-κB activation was decreased after treatment with fucoxanthin. Therefore, fucoxanthin may be used as anti-cervical cancer drugs in the future.</description><identifier>ISSN: 1010-4283</identifier><identifier>EISSN: 1423-0380</identifier><identifier>DOI: 10.1007/s13277-014-2337-7</identifier><identifier>PMID: 25113250</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Adenosine Triphosphate - metabolism ; Animals ; Apoptosis ; Apoptosis - drug effects ; Biomedical and Life Sciences ; Biomedicine ; Blotting, Western ; Cancer Research ; Cell Proliferation - drug effects ; Cervical cancer ; Female ; Flow Cytometry ; HeLa Cells ; Humans ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; NF-kappa B - metabolism ; Oxygen Consumption - drug effects ; Phosphatidylinositol 3-Kinase - metabolism ; Phosphorylation - drug effects ; Phytochemicals ; Proto-Oncogene Proteins c-akt - metabolism ; Research Article ; Signal transduction ; Signal Transduction - drug effects ; Tumor Cells, Cultured ; Uterine Cervical Neoplasms - drug therapy ; Uterine Cervical Neoplasms - metabolism ; Uterine Cervical Neoplasms - pathology ; Xanthophylls - pharmacology ; Xenograft Model Antitumor Assays</subject><ispartof>Tumor biology, 2014-11, Vol.35 (11), p.11261-11267</ispartof><rights>International Society of Oncology and BioMarkers (ISOBM) 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-758062188d828c2249083ce9a24baff26d06f1db58f4f7a1cef2ad7578f61ce3</citedby><cites>FETCH-LOGICAL-c442t-758062188d828c2249083ce9a24baff26d06f1db58f4f7a1cef2ad7578f61ce3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s13277-014-2337-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s13277-014-2337-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27925,27926,41489,42558,51320</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25113250$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ye, Guoliu</creatorcontrib><creatorcontrib>Lu, Qin</creatorcontrib><creatorcontrib>Zhao, Weidong</creatorcontrib><creatorcontrib>Du, Danli</creatorcontrib><creatorcontrib>Jin, Lijie</creatorcontrib><creatorcontrib>Liu, Yusheng</creatorcontrib><title>Fucoxanthin induces apoptosis in human cervical cancer cell line HeLa via PI3K/Akt pathway</title><title>Tumor biology</title><addtitle>Tumor Biol</addtitle><addtitle>Tumour Biol</addtitle><description>Cervical cancer (CC) is a malignant neoplasm arising from cells originating in the cervix uteri, among the top causes of death from cancer in women. In a gene expression profiling study of metabolic response to treatment, PI3K/Akt signaling pathway are associated with the development of CC. A common mechanism of Akt activation seen in cancer types is alterations in the upstream regulators of Akt such as phosphatidylinositol 3-kinase (PI3K), which is overexpressed in cervical cancer tissues, and leads to phosphorylation of Akt. Both PI3K and Akt inhibitors exist and may be therapeutically valuable. In the present study, we use MTT assay and western blot for the high-throughput screening to select specific inhibitors of PI3K/Akt signaling pathway, and then obtain fucoxanthin. Fucoxanthin is a water-soluble dietary fiber, taken from the unique slimy component of alginic cells. Various studies have pointed out that fucoxanthin is very effective for the treatment of cancer. Our results have shown that fucoxanthin induced a significant apoptosis of HeLa cells, compared with other candidates. After treatment with fucoxanthin for 24 h, the level of phosphorylation was inhibited in a dose-dependent manner, and the proteins of apoptotic markers were changed in HeLa cells. And fucoxanthin could suppress tumor growth in vivo. In addition, the mitochondrial signal transduction pathway maybe was involved in its mechanism and NF-κB activation was decreased after treatment with fucoxanthin. Therefore, fucoxanthin may be used as anti-cervical cancer drugs in the future.</description><subject>Adenosine Triphosphate - metabolism</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Blotting, Western</subject><subject>Cancer Research</subject><subject>Cell Proliferation - drug effects</subject><subject>Cervical cancer</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Nude</subject><subject>NF-kappa B - metabolism</subject><subject>Oxygen Consumption - drug effects</subject><subject>Phosphatidylinositol 3-Kinase - metabolism</subject><subject>Phosphorylation - drug effects</subject><subject>Phytochemicals</subject><subject>Proto-Oncogene Proteins c-akt - metabolism</subject><subject>Research Article</subject><subject>Signal transduction</subject><subject>Signal Transduction - drug effects</subject><subject>Tumor Cells, Cultured</subject><subject>Uterine Cervical Neoplasms - drug therapy</subject><subject>Uterine Cervical Neoplasms - metabolism</subject><subject>Uterine Cervical Neoplasms - pathology</subject><subject>Xanthophylls - pharmacology</subject><subject>Xenograft Model Antitumor Assays</subject><issn>1010-4283</issn><issn>1423-0380</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp1kL1OwzAURi0EoqXwACzIEgtLwD9J7IxVRWlFJRg6sViuY1OX1AlxUujb4ygFISQmX9vnfvfqAHCJ0S1GiN15TAljEcJxRChlETsCQxwTGiHK0XGoEUZRTDgdgDPvNwjhJMvSUzAgCQ6tCRqCl2mryk_pmrV10Lq8VdpDWZVVU3rrwwtct1vpoNL1zipZQCVdqMO9KGBhnYYzvZBwZyV8ntPHu_FbAyvZrD_k_hycGFl4fXE4R2A5vV9OZtHi6WE-GS8iFcekiVjCUUow5zknXBESZ4hTpTNJ4pU0hqQ5Sg3OVwk3sWESK22IzFnCuEnDhY7ATR9b1eV7q30jttZ360mny9YLnBKeBAeMBfT6D7op29qF5TqKZShM5oHCPaXq0vtaG1HVdivrvcBIdN5F710E76LzLrrkq0Nyu9rq_KfjW3QASA_48OVedf1r9L-pX0d8jCU</recordid><startdate>20141101</startdate><enddate>20141101</enddate><creator>Ye, Guoliu</creator><creator>Lu, Qin</creator><creator>Zhao, Weidong</creator><creator>Du, Danli</creator><creator>Jin, Lijie</creator><creator>Liu, Yusheng</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20141101</creationdate><title>Fucoxanthin induces apoptosis in human cervical cancer cell line HeLa via PI3K/Akt pathway</title><author>Ye, Guoliu ; Lu, Qin ; Zhao, Weidong ; Du, Danli ; Jin, Lijie ; Liu, Yusheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-758062188d828c2249083ce9a24baff26d06f1db58f4f7a1cef2ad7578f61ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Adenosine Triphosphate - 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Academic</collection><jtitle>Tumor biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ye, Guoliu</au><au>Lu, Qin</au><au>Zhao, Weidong</au><au>Du, Danli</au><au>Jin, Lijie</au><au>Liu, Yusheng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fucoxanthin induces apoptosis in human cervical cancer cell line HeLa via PI3K/Akt pathway</atitle><jtitle>Tumor biology</jtitle><stitle>Tumor Biol</stitle><addtitle>Tumour Biol</addtitle><date>2014-11-01</date><risdate>2014</risdate><volume>35</volume><issue>11</issue><spage>11261</spage><epage>11267</epage><pages>11261-11267</pages><issn>1010-4283</issn><eissn>1423-0380</eissn><abstract>Cervical cancer (CC) is a malignant neoplasm arising from cells originating in the cervix uteri, among the top causes of death from cancer in women. In a gene expression profiling study of metabolic response to treatment, PI3K/Akt signaling pathway are associated with the development of CC. A common mechanism of Akt activation seen in cancer types is alterations in the upstream regulators of Akt such as phosphatidylinositol 3-kinase (PI3K), which is overexpressed in cervical cancer tissues, and leads to phosphorylation of Akt. Both PI3K and Akt inhibitors exist and may be therapeutically valuable. In the present study, we use MTT assay and western blot for the high-throughput screening to select specific inhibitors of PI3K/Akt signaling pathway, and then obtain fucoxanthin. Fucoxanthin is a water-soluble dietary fiber, taken from the unique slimy component of alginic cells. Various studies have pointed out that fucoxanthin is very effective for the treatment of cancer. Our results have shown that fucoxanthin induced a significant apoptosis of HeLa cells, compared with other candidates. After treatment with fucoxanthin for 24 h, the level of phosphorylation was inhibited in a dose-dependent manner, and the proteins of apoptotic markers were changed in HeLa cells. And fucoxanthin could suppress tumor growth in vivo. In addition, the mitochondrial signal transduction pathway maybe was involved in its mechanism and NF-κB activation was decreased after treatment with fucoxanthin. Therefore, fucoxanthin may be used as anti-cervical cancer drugs in the future.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>25113250</pmid><doi>10.1007/s13277-014-2337-7</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adenosine Triphosphate - metabolism Animals Apoptosis Apoptosis - drug effects Biomedical and Life Sciences Biomedicine Blotting, Western Cancer Research Cell Proliferation - drug effects Cervical cancer Female Flow Cytometry HeLa Cells Humans Mice Mice, Inbred BALB C Mice, Nude NF-kappa B - metabolism Oxygen Consumption - drug effects Phosphatidylinositol 3-Kinase - metabolism Phosphorylation - drug effects Phytochemicals Proto-Oncogene Proteins c-akt - metabolism Research Article Signal transduction Signal Transduction - drug effects Tumor Cells, Cultured Uterine Cervical Neoplasms - drug therapy Uterine Cervical Neoplasms - metabolism Uterine Cervical Neoplasms - pathology Xanthophylls - pharmacology Xenograft Model Antitumor Assays |
| title | Fucoxanthin induces apoptosis in human cervical cancer cell line HeLa via PI3K/Akt pathway |
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