Genomic organization, chromosomal localization, tissue distribution, and biophysical characterization of a novel mammalian Shaker-related voltage-gated potassium channel, Kv1.7
We report the isolation of a novel mouse voltage-gated Shaker-related K+ channel gene, Kv1.7 (Kcna7/KCNA7). Unlike other known Kv1 family genes that have intronless coding regions, the protein-coding region of Kv1.7 is interrupted by a 1.9-kilobase pair intron. The Kv1.7 gene and the related Kv3.3 (...
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| Veröffentlicht in: | The Journal of biological chemistry 1998-03, Vol.273 (10), p.5851-5857 |
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| creator | Kalman, K Nguyen, A Tseng-Crank, J Dukes, I D Chandy, G Hustad, C M Copeland, N G Jenkins, N A Mohrenweiser, H Brandriff, B Cahalan, M Gutman, G A Chandy, K G |
| description | We report the isolation of a novel mouse voltage-gated Shaker-related K+ channel gene, Kv1.7 (Kcna7/KCNA7). Unlike other known Kv1 family genes that have intronless coding regions, the protein-coding region of Kv1.7 is interrupted by a 1.9-kilobase pair intron. The Kv1.7 gene and the related Kv3.3 (Kcnc3/KCNC3) gene map to mouse chromosome 7 and human chromosome 19q13.3, a region that has been suggested to contain a diabetic susceptibility locus. The mouse Kv1.7 channel is voltage-dependent and rapidly inactivating, exhibits cumulative inactivation, and has a single channel conductance of 21 pS. It is potently blocked by noxiustoxin and stichodactylatoxin, and is insensitive to tetraethylammonium, kaliotoxin, and charybdotoxin. Northern blot analysis reveals approximately 3-kilobase pair Kv1.7 transcripts in mouse heart and skeletal muscle. In situ hybridization demonstrates the presence of Kv1.7 in mouse pancreatic islet cells. Kv1.7 was also isolated from mouse brain and hamster insulinoma cells by polymerase chain reaction. |
| doi_str_mv | 10.1074/jbc.273.10.5851 |
| format | Article |
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Unlike other known Kv1 family genes that have intronless coding regions, the protein-coding region of Kv1.7 is interrupted by a 1.9-kilobase pair intron. The Kv1.7 gene and the related Kv3.3 (Kcnc3/KCNC3) gene map to mouse chromosome 7 and human chromosome 19q13.3, a region that has been suggested to contain a diabetic susceptibility locus. The mouse Kv1.7 channel is voltage-dependent and rapidly inactivating, exhibits cumulative inactivation, and has a single channel conductance of 21 pS. It is potently blocked by noxiustoxin and stichodactylatoxin, and is insensitive to tetraethylammonium, kaliotoxin, and charybdotoxin. Northern blot analysis reveals approximately 3-kilobase pair Kv1.7 transcripts in mouse heart and skeletal muscle. In situ hybridization demonstrates the presence of Kv1.7 in mouse pancreatic islet cells. Kv1.7 was also isolated from mouse brain and hamster insulinoma cells by polymerase chain reaction.</description><identifier>ISSN: 0021-9258</identifier><identifier>DOI: 10.1074/jbc.273.10.5851</identifier><identifier>PMID: 9488722</identifier><language>eng</language><publisher>United States</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Chromosome Mapping ; Chromosomes ; Cloning, Molecular ; Electrophysiology ; Humans ; In Situ Hybridization ; Ion Channel Gating - physiology ; Islets of Langerhans - cytology ; Islets of Langerhans - physiology ; Mice ; Mice, Inbred Strains ; Molecular Sequence Data ; Neurotoxins - pharmacology ; Phylogeny ; Potassium Channels - chemistry ; Potassium Channels - genetics ; Sequence Analysis, DNA ; Shaker Superfamily of Potassium Channels</subject><ispartof>The Journal of biological chemistry, 1998-03, Vol.273 (10), p.5851-5857</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9488722$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kalman, K</creatorcontrib><creatorcontrib>Nguyen, A</creatorcontrib><creatorcontrib>Tseng-Crank, J</creatorcontrib><creatorcontrib>Dukes, I D</creatorcontrib><creatorcontrib>Chandy, G</creatorcontrib><creatorcontrib>Hustad, C M</creatorcontrib><creatorcontrib>Copeland, N G</creatorcontrib><creatorcontrib>Jenkins, N A</creatorcontrib><creatorcontrib>Mohrenweiser, H</creatorcontrib><creatorcontrib>Brandriff, B</creatorcontrib><creatorcontrib>Cahalan, M</creatorcontrib><creatorcontrib>Gutman, G A</creatorcontrib><creatorcontrib>Chandy, K G</creatorcontrib><title>Genomic organization, chromosomal localization, tissue distribution, and biophysical characterization of a novel mammalian Shaker-related voltage-gated potassium channel, Kv1.7</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>We report the isolation of a novel mouse voltage-gated Shaker-related K+ channel gene, Kv1.7 (Kcna7/KCNA7). Unlike other known Kv1 family genes that have intronless coding regions, the protein-coding region of Kv1.7 is interrupted by a 1.9-kilobase pair intron. The Kv1.7 gene and the related Kv3.3 (Kcnc3/KCNC3) gene map to mouse chromosome 7 and human chromosome 19q13.3, a region that has been suggested to contain a diabetic susceptibility locus. The mouse Kv1.7 channel is voltage-dependent and rapidly inactivating, exhibits cumulative inactivation, and has a single channel conductance of 21 pS. It is potently blocked by noxiustoxin and stichodactylatoxin, and is insensitive to tetraethylammonium, kaliotoxin, and charybdotoxin. Northern blot analysis reveals approximately 3-kilobase pair Kv1.7 transcripts in mouse heart and skeletal muscle. In situ hybridization demonstrates the presence of Kv1.7 in mouse pancreatic islet cells. Kv1.7 was also isolated from mouse brain and hamster insulinoma cells by polymerase chain reaction.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Chromosome Mapping</subject><subject>Chromosomes</subject><subject>Cloning, Molecular</subject><subject>Electrophysiology</subject><subject>Humans</subject><subject>In Situ Hybridization</subject><subject>Ion Channel Gating - physiology</subject><subject>Islets of Langerhans - cytology</subject><subject>Islets of Langerhans - physiology</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>Molecular Sequence Data</subject><subject>Neurotoxins - pharmacology</subject><subject>Phylogeny</subject><subject>Potassium Channels - chemistry</subject><subject>Potassium Channels - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Shaker Superfamily of Potassium Channels</subject><issn>0021-9258</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kLtOAzEQRV2AIDxqKiRXVNlgezdZp0QRLxGJAqijWe9sYvBjsb2R4Kv4RAxETDGjO3PuLYaQM84mnNXV5WujJqIus5hM5ZTvkRFjghdzMZWH5CjGV5armvMDcjCvpKyFGJGvW3TeakV9WIPTn5C0d2OqNsFbH70FQ41XYP4vScc4IG11TEE3w98SXEsb7fvNR9QZznYIoBKGnY36jgJ1fouGWrA5VYOjTxt4w1AENJCwpVtvEqyxWP-q3ieIUQ_2J8w5NGP6sOWT-oTsd2Ainu7mMXm5uX5e3BXLx9v7xdWy6PlsngrZdZ0spWISZK1mTAmJLcxUmXs-AGOILEOy5qyUAFiV05o3mexY2bVQHpOLv9w--PcBY1pZHRUaAw79EFd8JmQlBM_g-Q4cGovtqg_aQvhY7V5cfgNgWYHT</recordid><startdate>19980306</startdate><enddate>19980306</enddate><creator>Kalman, K</creator><creator>Nguyen, A</creator><creator>Tseng-Crank, J</creator><creator>Dukes, I D</creator><creator>Chandy, G</creator><creator>Hustad, C M</creator><creator>Copeland, N G</creator><creator>Jenkins, N A</creator><creator>Mohrenweiser, H</creator><creator>Brandriff, B</creator><creator>Cahalan, M</creator><creator>Gutman, G A</creator><creator>Chandy, K G</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19980306</creationdate><title>Genomic organization, chromosomal localization, tissue distribution, and biophysical characterization of a novel mammalian Shaker-related voltage-gated potassium channel, Kv1.7</title><author>Kalman, K ; Nguyen, A ; Tseng-Crank, J ; Dukes, I D ; Chandy, G ; Hustad, C M ; Copeland, N G ; Jenkins, N A ; Mohrenweiser, H ; Brandriff, B ; Cahalan, M ; Gutman, G A ; Chandy, K G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p169t-8fff838c08a87c60c28eda6c3edaf83a00ee08ff871038aae43571bc60f03fda3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Chromosome Mapping</topic><topic>Chromosomes</topic><topic>Cloning, Molecular</topic><topic>Electrophysiology</topic><topic>Humans</topic><topic>In Situ Hybridization</topic><topic>Ion Channel Gating - physiology</topic><topic>Islets of Langerhans - cytology</topic><topic>Islets of Langerhans - physiology</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>Molecular Sequence Data</topic><topic>Neurotoxins - pharmacology</topic><topic>Phylogeny</topic><topic>Potassium Channels - chemistry</topic><topic>Potassium Channels - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Shaker Superfamily of Potassium Channels</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kalman, K</creatorcontrib><creatorcontrib>Nguyen, A</creatorcontrib><creatorcontrib>Tseng-Crank, J</creatorcontrib><creatorcontrib>Dukes, I D</creatorcontrib><creatorcontrib>Chandy, G</creatorcontrib><creatorcontrib>Hustad, C M</creatorcontrib><creatorcontrib>Copeland, N G</creatorcontrib><creatorcontrib>Jenkins, N A</creatorcontrib><creatorcontrib>Mohrenweiser, H</creatorcontrib><creatorcontrib>Brandriff, B</creatorcontrib><creatorcontrib>Cahalan, M</creatorcontrib><creatorcontrib>Gutman, G A</creatorcontrib><creatorcontrib>Chandy, K G</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kalman, K</au><au>Nguyen, A</au><au>Tseng-Crank, J</au><au>Dukes, I D</au><au>Chandy, G</au><au>Hustad, C M</au><au>Copeland, N G</au><au>Jenkins, N A</au><au>Mohrenweiser, H</au><au>Brandriff, B</au><au>Cahalan, M</au><au>Gutman, G A</au><au>Chandy, K G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genomic organization, chromosomal localization, tissue distribution, and biophysical characterization of a novel mammalian Shaker-related voltage-gated potassium channel, Kv1.7</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1998-03-06</date><risdate>1998</risdate><volume>273</volume><issue>10</issue><spage>5851</spage><epage>5857</epage><pages>5851-5857</pages><issn>0021-9258</issn><abstract>We report the isolation of a novel mouse voltage-gated Shaker-related K+ channel gene, Kv1.7 (Kcna7/KCNA7). Unlike other known Kv1 family genes that have intronless coding regions, the protein-coding region of Kv1.7 is interrupted by a 1.9-kilobase pair intron. The Kv1.7 gene and the related Kv3.3 (Kcnc3/KCNC3) gene map to mouse chromosome 7 and human chromosome 19q13.3, a region that has been suggested to contain a diabetic susceptibility locus. The mouse Kv1.7 channel is voltage-dependent and rapidly inactivating, exhibits cumulative inactivation, and has a single channel conductance of 21 pS. It is potently blocked by noxiustoxin and stichodactylatoxin, and is insensitive to tetraethylammonium, kaliotoxin, and charybdotoxin. Northern blot analysis reveals approximately 3-kilobase pair Kv1.7 transcripts in mouse heart and skeletal muscle. In situ hybridization demonstrates the presence of Kv1.7 in mouse pancreatic islet cells. 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| ispartof | The Journal of biological chemistry, 1998-03, Vol.273 (10), p.5851-5857 |
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| subjects | Amino Acid Sequence Animals Base Sequence Chromosome Mapping Chromosomes Cloning, Molecular Electrophysiology Humans In Situ Hybridization Ion Channel Gating - physiology Islets of Langerhans - cytology Islets of Langerhans - physiology Mice Mice, Inbred Strains Molecular Sequence Data Neurotoxins - pharmacology Phylogeny Potassium Channels - chemistry Potassium Channels - genetics Sequence Analysis, DNA Shaker Superfamily of Potassium Channels |
| title | Genomic organization, chromosomal localization, tissue distribution, and biophysical characterization of a novel mammalian Shaker-related voltage-gated potassium channel, Kv1.7 |
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