Evaluation of bifidobacterial community composition in the human gut by means of a targeted amplicon sequencing (ITS) protocol

Abstract The precise appraisal of the composition of the human gut microbiota still represents a challenging task. The advent of next generation sequencing approaches has opened new ways to dissect the microbial biodiversity of this ecosystem through the use of 16S rRNA gene-based microbiota analysi...

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Veröffentlicht in:	FEMS microbiology ecology 2014-11, Vol.90 (2), p.493-503
Hauptverfasser:	Milani, Christian, Lugli, Gabriele A., Turroni, Francesca, Mancabelli, Leonardo, Duranti, Sabrina, Viappiani, Alice, Mangifesta, Marta, Segata, Nicola, van Sinderen, Douwe, Ventura, Marco
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Bifidobacteria Bifidobacterium Bifidobacterium - classification Bifidobacterium - genetics Bifidobacterium - isolation & purification Biodiversity Community composition DNA, Bacterial - genetics DNA, Ribosomal Spacer - genetics Ecology Feces - microbiology Gastrointestinal Tract - microbiology Genes High-Throughput Nucleotide Sequencing Humans Infant Ion Torrent ITS Microbiology Molecular Sequence Data Phylogeny Polymerase Chain Reaction - methods protocols RNA, Ribosomal, 16S - genetics
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