Production of functional inclusion bodies in endotoxin-free Escherichia coli

Escherichia coli is the workhorse for gene cloning and production of soluble recombinant proteins in both biotechnological and biomedical industries. The bacterium is also a good producer of several classes of protein-based self-assembling materials such as inclusion bodies (IBs). Apart from being a...

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Veröffentlicht in:Applied microbiology and biotechnology 2014-11, Vol.98 (22), p.9229-9238
Hauptverfasser: Rueda, Fabián, Cano-Garrido, Olivia, Mamat, Uwe, Wilke, Kathleen, Seras-Franzoso, Joaquin, García-Fruitós, Elena, Villaverde, Antonio
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container_end_page 9238
container_issue 22
container_start_page 9229
container_title Applied microbiology and biotechnology
container_volume 98
creator Rueda, Fabián
Cano-Garrido, Olivia
Mamat, Uwe
Wilke, Kathleen
Seras-Franzoso, Joaquin
García-Fruitós, Elena
Villaverde, Antonio
description Escherichia coli is the workhorse for gene cloning and production of soluble recombinant proteins in both biotechnological and biomedical industries. The bacterium is also a good producer of several classes of protein-based self-assembling materials such as inclusion bodies (IBs). Apart from being a relatively pure source of protein for in vitro refolding, IBs are under exploration as functional, protein-releasing materials in regenerative medicine and protein replacement therapies. Endotoxin removal is a critical step for downstream applications of therapeutic proteins. The same holds true for IBs as they are often highly contaminated with cell-wall components of the host cells. Here, we have investigated the production of IBs in a recently developed endotoxin-free E. coli strain. The characterization of IBs revealed this mutant as a very useful cell factory for the production of functional endotoxin-free IBs that are suitable for the use at biological interfaces without inducing endotoxic responses in human immune cells.
doi_str_mv 10.1007/s00253-014-6008-9
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subjects bacteria
Biological Products - metabolism
Biomedical and Life Sciences
biopharmaceuticals
Biotechnological Products and Process Engineering
Biotechnology
Biotechnology - methods
Cloning
Cold
E coli
Endotoxins
Endotoxins - deficiency
Escherichia coli
Escherichia coli - metabolism
Gene expression
Genetic recombination
Genetics
Glycerol
Health aspects
humans
inclusion bodies
Inclusion Bodies - metabolism
industry
Life Sciences
Mammals
medicine
Methods
Microbial Genetics and Genomics
Microbiological synthesis
Microbiology
molecular cloning
mutants
Nanostructured materials
Physiological aspects
Plasmids
Production processes
Proteins
Recombinant proteins
Recombinant Proteins - metabolism
Studies
Technology, Pharmaceutical - methods
Toxins
title Production of functional inclusion bodies in endotoxin-free Escherichia coli
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