Lgr5 expression as stem cell marker in human gastric gland and its relatedness with other putative cancer stem cell markers
To explore Lgr5 as the possible stem cell marker in human gastric tissue, 259 normal gastric tissues and dissected gastric adenocarcinoma were analyzed by immunohistochemistry, immunofluorescence double staining and qRT-PCR. The results demonstrated that Lgr5 was expressed in the bottom of the norma...
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| Veröffentlicht in: | Gene 2013-08, Vol.525 (1), p.18-25 |
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| creator | Wu, Chen Xie, Yuanyuan Gao, Feng Wang, Yanan Guo, Yawei Tian, Huanna Li, Yanshuang Fan, Wufang |
| description | To explore Lgr5 as the possible stem cell marker in human gastric tissue, 259 normal gastric tissues and dissected gastric adenocarcinoma were analyzed by immunohistochemistry, immunofluorescence double staining and qRT-PCR. The results demonstrated that Lgr5 was expressed in the bottom of the normal gastric gland units, and showed a differential expression in gastric adenocarcinoma with varying differentiation. Lgr5 and Bmi1 were co-expressed within the same cells of gastric glands. CD26+, CD44+, ALDH1+ and CD133+ cells co-existed with Lgr5+ cells in the stem cell zone of adjacent normal gastric mucosa, and they were detectable in gastric adenocarcinoma but behaved differently. We concluded that Lgr5 may be the adult stem cell marker in human gastric epithelium; Lgr5 and Bmi1 may belong to the same stem cell population; Lgr5, CD26, CD44, ALDH1, and CD133 may be functionally-associated.
•Lgr5+ cells exist at base of gland units in normal gastric mucosa.•Lgr5 was differentially expressed in varying stages of differentiation of cancer.•Lgr5 and Bmi1 were co-expressed within the same cells of gastric glands.•CD26+, CD44+, ALDH1+ and CD133+ cells co-existed with Lgr5+ cells. |
| doi_str_mv | 10.1016/j.gene.2013.04.067 |
| format | Article |
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•Lgr5+ cells exist at base of gland units in normal gastric mucosa.•Lgr5 was differentially expressed in varying stages of differentiation of cancer.•Lgr5 and Bmi1 were co-expressed within the same cells of gastric glands.•CD26+, CD44+, ALDH1+ and CD133+ cells co-existed with Lgr5+ cells.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/j.gene.2013.04.067</identifier><identifier>PMID: 23664892</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>adenocarcinoma ; Adenocarcinoma - genetics ; Adenocarcinoma - metabolism ; Adenocarcinoma - pathology ; adults ; Antigens, CD - genetics ; Antigens, CD - metabolism ; Biomarkers, Tumor - biosynthesis ; Biomarkers, Tumor - genetics ; Biomarkers, Tumor - metabolism ; Bmi1 ; Cancer stem cells ; Cell Differentiation - genetics ; Epithelium - metabolism ; fluorescent antibody technique ; Gastric adenocarcinoma ; gastric mucosa ; Gastric Mucosa - metabolism ; gene expression regulation ; Humans ; Immunohistochemistry ; Isoenzymes - genetics ; Isoenzymes - metabolism ; Lgr5 ; Neoplastic Stem Cells - cytology ; Neoplastic Stem Cells - metabolism ; Polycomb Repressive Complex 1 - genetics ; Polycomb Repressive Complex 1 - metabolism ; Receptors, G-Protein-Coupled - biosynthesis ; Receptors, G-Protein-Coupled - genetics ; Receptors, G-Protein-Coupled - metabolism ; Retinal Dehydrogenase - genetics ; Retinal Dehydrogenase - metabolism ; Retrospective Studies ; reverse transcriptase polymerase chain reaction ; stem cells ; Stem Cells - cytology ; Stem Cells - metabolism ; Stomach Neoplasms - genetics ; Stomach Neoplasms - metabolism ; Stomach Neoplasms - pathology</subject><ispartof>Gene, 2013-08, Vol.525 (1), p.18-25</ispartof><rights>2013 Elsevier B.V.</rights><rights>Copyright © 2013 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-b88a34459d0c1d686c63821638c92bc8fef0048aefb3c5b4c0452b239ff697653</citedby><cites>FETCH-LOGICAL-c413t-b88a34459d0c1d686c63821638c92bc8fef0048aefb3c5b4c0452b239ff697653</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.gene.2013.04.067$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23664892$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Chen</creatorcontrib><creatorcontrib>Xie, Yuanyuan</creatorcontrib><creatorcontrib>Gao, Feng</creatorcontrib><creatorcontrib>Wang, Yanan</creatorcontrib><creatorcontrib>Guo, Yawei</creatorcontrib><creatorcontrib>Tian, Huanna</creatorcontrib><creatorcontrib>Li, Yanshuang</creatorcontrib><creatorcontrib>Fan, Wufang</creatorcontrib><title>Lgr5 expression as stem cell marker in human gastric gland and its relatedness with other putative cancer stem cell markers</title><title>Gene</title><addtitle>Gene</addtitle><description>To explore Lgr5 as the possible stem cell marker in human gastric tissue, 259 normal gastric tissues and dissected gastric adenocarcinoma were analyzed by immunohistochemistry, immunofluorescence double staining and qRT-PCR. The results demonstrated that Lgr5 was expressed in the bottom of the normal gastric gland units, and showed a differential expression in gastric adenocarcinoma with varying differentiation. Lgr5 and Bmi1 were co-expressed within the same cells of gastric glands. CD26+, CD44+, ALDH1+ and CD133+ cells co-existed with Lgr5+ cells in the stem cell zone of adjacent normal gastric mucosa, and they were detectable in gastric adenocarcinoma but behaved differently. We concluded that Lgr5 may be the adult stem cell marker in human gastric epithelium; Lgr5 and Bmi1 may belong to the same stem cell population; Lgr5, CD26, CD44, ALDH1, and CD133 may be functionally-associated.
•Lgr5+ cells exist at base of gland units in normal gastric mucosa.•Lgr5 was differentially expressed in varying stages of differentiation of cancer.•Lgr5 and Bmi1 were co-expressed within the same cells of gastric glands.•CD26+, CD44+, ALDH1+ and CD133+ cells co-existed with Lgr5+ cells.</description><subject>adenocarcinoma</subject><subject>Adenocarcinoma - genetics</subject><subject>Adenocarcinoma - metabolism</subject><subject>Adenocarcinoma - pathology</subject><subject>adults</subject><subject>Antigens, CD - genetics</subject><subject>Antigens, CD - metabolism</subject><subject>Biomarkers, Tumor - biosynthesis</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Bmi1</subject><subject>Cancer stem cells</subject><subject>Cell Differentiation - genetics</subject><subject>Epithelium - metabolism</subject><subject>fluorescent antibody technique</subject><subject>Gastric adenocarcinoma</subject><subject>gastric mucosa</subject><subject>Gastric Mucosa - metabolism</subject><subject>gene expression regulation</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Isoenzymes - genetics</subject><subject>Isoenzymes - metabolism</subject><subject>Lgr5</subject><subject>Neoplastic Stem Cells - cytology</subject><subject>Neoplastic Stem Cells - metabolism</subject><subject>Polycomb Repressive Complex 1 - genetics</subject><subject>Polycomb Repressive Complex 1 - metabolism</subject><subject>Receptors, G-Protein-Coupled - biosynthesis</subject><subject>Receptors, G-Protein-Coupled - genetics</subject><subject>Receptors, G-Protein-Coupled - metabolism</subject><subject>Retinal Dehydrogenase - genetics</subject><subject>Retinal Dehydrogenase - metabolism</subject><subject>Retrospective Studies</subject><subject>reverse transcriptase polymerase chain reaction</subject><subject>stem cells</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - metabolism</subject><subject>Stomach Neoplasms - genetics</subject><subject>Stomach Neoplasms - metabolism</subject><subject>Stomach Neoplasms - pathology</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUuLFDEUhYMoTs_oH3ChWbqpMu9KwI0MOgoNLnTWIZW6VZ22Hm2SGhX_vCl6dOFCA0kIfOdwcw5CzyipKaHq1bEeYIaaEcprImqimgdoR3VjKkK4foh2hDe6opSaC3SZ0pGUJSV7jC4YV0pow3bo536IEsP3U4SUwjJjl3DKMGEP44gnF79AxGHGh3VyMx5cyjF4PIxu7vC2Q044wugydHNxwN9CPuAlH4rqtGaXwx1g72Zf3n_bpifoUe_GBE_v7yt0--7t5-v31f7jzYfrN_vKC8pz1WrtuBDSdMTTTmnlFdeMlsMb1nrdQ0-I0A76lnvZCk-EZC3jpu-VaZTkV-jl2fcUl68rpGynkLZB3AzLmixVrDGaCKr_j5bgZNM01BSUnVEfl5Qi9PYUQ_nZD0uJ3fqxR7v1Y7d-LBG29FNEz-_913aC7o_kdyEFeHEGerdYN8SQ7O2n4iBLdyUDTQrx-kxAiewuQLTJBygJdyGCz7Zbwr8m-AVmRqsN</recordid><startdate>20130801</startdate><enddate>20130801</enddate><creator>Wu, Chen</creator><creator>Xie, Yuanyuan</creator><creator>Gao, Feng</creator><creator>Wang, Yanan</creator><creator>Guo, Yawei</creator><creator>Tian, Huanna</creator><creator>Li, Yanshuang</creator><creator>Fan, Wufang</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20130801</creationdate><title>Lgr5 expression as stem cell marker in human gastric gland and its relatedness with other putative cancer stem cell markers</title><author>Wu, Chen ; Xie, Yuanyuan ; Gao, Feng ; Wang, Yanan ; Guo, Yawei ; Tian, Huanna ; Li, Yanshuang ; Fan, Wufang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-b88a34459d0c1d686c63821638c92bc8fef0048aefb3c5b4c0452b239ff697653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>adenocarcinoma</topic><topic>Adenocarcinoma - genetics</topic><topic>Adenocarcinoma - metabolism</topic><topic>Adenocarcinoma - pathology</topic><topic>adults</topic><topic>Antigens, CD - genetics</topic><topic>Antigens, CD - metabolism</topic><topic>Biomarkers, Tumor - biosynthesis</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Bmi1</topic><topic>Cancer stem cells</topic><topic>Cell Differentiation - genetics</topic><topic>Epithelium - metabolism</topic><topic>fluorescent antibody technique</topic><topic>Gastric adenocarcinoma</topic><topic>gastric mucosa</topic><topic>Gastric Mucosa - metabolism</topic><topic>gene expression regulation</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Isoenzymes - genetics</topic><topic>Isoenzymes - metabolism</topic><topic>Lgr5</topic><topic>Neoplastic Stem Cells - cytology</topic><topic>Neoplastic Stem Cells - metabolism</topic><topic>Polycomb Repressive Complex 1 - genetics</topic><topic>Polycomb Repressive Complex 1 - metabolism</topic><topic>Receptors, G-Protein-Coupled - biosynthesis</topic><topic>Receptors, G-Protein-Coupled - genetics</topic><topic>Receptors, G-Protein-Coupled - metabolism</topic><topic>Retinal Dehydrogenase - genetics</topic><topic>Retinal Dehydrogenase - metabolism</topic><topic>Retrospective Studies</topic><topic>reverse transcriptase polymerase chain reaction</topic><topic>stem cells</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - metabolism</topic><topic>Stomach Neoplasms - genetics</topic><topic>Stomach Neoplasms - metabolism</topic><topic>Stomach Neoplasms - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Chen</creatorcontrib><creatorcontrib>Xie, Yuanyuan</creatorcontrib><creatorcontrib>Gao, Feng</creatorcontrib><creatorcontrib>Wang, Yanan</creatorcontrib><creatorcontrib>Guo, Yawei</creatorcontrib><creatorcontrib>Tian, Huanna</creatorcontrib><creatorcontrib>Li, Yanshuang</creatorcontrib><creatorcontrib>Fan, Wufang</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Chen</au><au>Xie, Yuanyuan</au><au>Gao, Feng</au><au>Wang, Yanan</au><au>Guo, Yawei</au><au>Tian, Huanna</au><au>Li, Yanshuang</au><au>Fan, Wufang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lgr5 expression as stem cell marker in human gastric gland and its relatedness with other putative cancer stem cell markers</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>2013-08-01</date><risdate>2013</risdate><volume>525</volume><issue>1</issue><spage>18</spage><epage>25</epage><pages>18-25</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>To explore Lgr5 as the possible stem cell marker in human gastric tissue, 259 normal gastric tissues and dissected gastric adenocarcinoma were analyzed by immunohistochemistry, immunofluorescence double staining and qRT-PCR. The results demonstrated that Lgr5 was expressed in the bottom of the normal gastric gland units, and showed a differential expression in gastric adenocarcinoma with varying differentiation. Lgr5 and Bmi1 were co-expressed within the same cells of gastric glands. CD26+, CD44+, ALDH1+ and CD133+ cells co-existed with Lgr5+ cells in the stem cell zone of adjacent normal gastric mucosa, and they were detectable in gastric adenocarcinoma but behaved differently. We concluded that Lgr5 may be the adult stem cell marker in human gastric epithelium; Lgr5 and Bmi1 may belong to the same stem cell population; Lgr5, CD26, CD44, ALDH1, and CD133 may be functionally-associated.
•Lgr5+ cells exist at base of gland units in normal gastric mucosa.•Lgr5 was differentially expressed in varying stages of differentiation of cancer.•Lgr5 and Bmi1 were co-expressed within the same cells of gastric glands.•CD26+, CD44+, ALDH1+ and CD133+ cells co-existed with Lgr5+ cells.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>23664892</pmid><doi>10.1016/j.gene.2013.04.067</doi><tpages>8</tpages></addata></record> |
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| subjects | adenocarcinoma Adenocarcinoma - genetics Adenocarcinoma - metabolism Adenocarcinoma - pathology adults Antigens, CD - genetics Antigens, CD - metabolism Biomarkers, Tumor - biosynthesis Biomarkers, Tumor - genetics Biomarkers, Tumor - metabolism Bmi1 Cancer stem cells Cell Differentiation - genetics Epithelium - metabolism fluorescent antibody technique Gastric adenocarcinoma gastric mucosa Gastric Mucosa - metabolism gene expression regulation Humans Immunohistochemistry Isoenzymes - genetics Isoenzymes - metabolism Lgr5 Neoplastic Stem Cells - cytology Neoplastic Stem Cells - metabolism Polycomb Repressive Complex 1 - genetics Polycomb Repressive Complex 1 - metabolism Receptors, G-Protein-Coupled - biosynthesis Receptors, G-Protein-Coupled - genetics Receptors, G-Protein-Coupled - metabolism Retinal Dehydrogenase - genetics Retinal Dehydrogenase - metabolism Retrospective Studies reverse transcriptase polymerase chain reaction stem cells Stem Cells - cytology Stem Cells - metabolism Stomach Neoplasms - genetics Stomach Neoplasms - metabolism Stomach Neoplasms - pathology |
| title | Lgr5 expression as stem cell marker in human gastric gland and its relatedness with other putative cancer stem cell markers |
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