Monoclonal antibody proteomics: Use of antibody mimotope displaying phages and the relevant synthetic peptides for mAb scouting

Highlights • A workflow for selecting monoclonal antibodies for biomarker assays was devised. • Large mAB libraries where cognate antigens are not known were used. • Phage display and SPR were applied to determine key features of antibodies. • Diversity of the selected peptides inversely correlated...

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Veröffentlicht in:	Immunology letters 2014-08, Vol.160 (2), p.172-177
Hauptverfasser:	Hajdú, István, Flachner, Beáta, Bognár, Melinda, Végh, Barbara M, Dobi, Krisztina, Lőrincz, Zsolt, Lázár, József, Cseh, Sándor, Takács, László, Kurucz, István
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity Allergy and Immunology Amino Acid Sequence Antibodies, Monoclonal - chemistry Antibodies, Monoclonal - genetics Antibodies, Monoclonal - immunology Epitopes - chemistry Epitopes - genetics Epitopes - immunology Gene Expression Humans Immunoassay Immunoglobulins - blood Immunoglobulins - genetics Immunoglobulins - immunology Kinetics Molecular Sequence Data Monoclonal antibody Peptide Library Peptides - chemistry Peptides - genetics Peptides - immunology Phage-display Protein Binding Proteomics Proteomics - methods Surface Plasmon Resonance
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Beschreibung
Zusammenfassung:	Highlights • A workflow for selecting monoclonal antibodies for biomarker assays was devised. • Large mAB libraries where cognate antigens are not known were used. • Phage display and SPR were applied to determine key features of antibodies. • Diversity of the selected peptides inversely correlated antibodies’ off-rate. • Using synthetic peptides and complex protein mixtures, antibody specificity can be tested.
ISSN:	0165-2478 1879-0542
DOI:	10.1016/j.imlet.2014.02.001