Synchrotron radiation based STXM analysis and micro-XRF mapping of differential expression of extracellular thiol groups by Acidithiobacillus ferrooxidans grown on Fe2+ and S0
The differential expression of extracellular thiol groups by Acidithiobacillus ferrooxidans grown on substrates Fe2+ and S0 was investigated by using synchrotron radiation based scanning transmission X-ray microscopy (STXM) imaging and microbeam X-ray fluorescence (μ-XRF) mapping. The extracellular...
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| Veröffentlicht in: | Journal of microbiological methods 2013-09, Vol.94 (3), p.257-261 |
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| creator | Xia, Jin-Lan Liu, Hong-Chang Nie, Zhen-Yuan Peng, An-An Zhen, Xiang-Jun Yang, Yun Zhang, Xiu-Li |
| description | The differential expression of extracellular thiol groups by Acidithiobacillus ferrooxidans grown on substrates Fe2+ and S0 was investigated by using synchrotron radiation based scanning transmission X-ray microscopy (STXM) imaging and microbeam X-ray fluorescence (μ-XRF) mapping. The extracellular thiol groups (SH) were first alkylated by iodoacetic acid forming Protein-SCH2COOH and then the P-SCH2COOH was marked by calcium ions forming P-SCH2COOCa. The STXM imaging and μ-XRF mapping of SH were based on analysis of SCH2COO-bonded Ca2+. The results indicated that the thiol group content of A. ferrooxidans grown on S0 is 3.88 times to that on Fe2+. Combined with selective labeling of SH by Ca2+, the STXM imaging and μ-XRF mapping provided an in situ and rapid analysis of differential expression of extracellular thiol groups.
•STXM imaging and m-XRF mapping were firstly used for thiol group analysis.•The membrane thiol groups were selectively labeled with Ca2+.•STXM image provided an in situ evaluation of extracellular thiol groups.•Micro-XRF mapping was rapid to determine the differential expression of thiol groups.•The -SH content of A. ferrooxidans grown on S0 was 3.88 times more to that on Fe2+. |
| doi_str_mv | 10.1016/j.mimet.2013.06.030 |
| format | Article |
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•STXM imaging and m-XRF mapping were firstly used for thiol group analysis.•The membrane thiol groups were selectively labeled with Ca2+.•STXM image provided an in situ evaluation of extracellular thiol groups.•Micro-XRF mapping was rapid to determine the differential expression of thiol groups.•The -SH content of A. ferrooxidans grown on S0 was 3.88 times more to that on Fe2+.</description><identifier>ISSN: 0167-7012</identifier><identifier>EISSN: 1872-8359</identifier><identifier>DOI: 10.1016/j.mimet.2013.06.030</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>Acidithiobacillus ferrooxidans ; Extracellular thiol group ; Micro-XRF mapping ; STXM imaging ; Synchrotron radiation</subject><ispartof>Journal of microbiological methods, 2013-09, Vol.94 (3), p.257-261</ispartof><rights>2013 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1812-c37ddde269f3b8326937cf5d19b28b093e1c488e513b1e79c38787746827122c3</citedby><cites>FETCH-LOGICAL-c1812-c37ddde269f3b8326937cf5d19b28b093e1c488e513b1e79c38787746827122c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.mimet.2013.06.030$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3541,27915,27916,45986</link.rule.ids></links><search><creatorcontrib>Xia, Jin-Lan</creatorcontrib><creatorcontrib>Liu, Hong-Chang</creatorcontrib><creatorcontrib>Nie, Zhen-Yuan</creatorcontrib><creatorcontrib>Peng, An-An</creatorcontrib><creatorcontrib>Zhen, Xiang-Jun</creatorcontrib><creatorcontrib>Yang, Yun</creatorcontrib><creatorcontrib>Zhang, Xiu-Li</creatorcontrib><title>Synchrotron radiation based STXM analysis and micro-XRF mapping of differential expression of extracellular thiol groups by Acidithiobacillus ferrooxidans grown on Fe2+ and S0</title><title>Journal of microbiological methods</title><description>The differential expression of extracellular thiol groups by Acidithiobacillus ferrooxidans grown on substrates Fe2+ and S0 was investigated by using synchrotron radiation based scanning transmission X-ray microscopy (STXM) imaging and microbeam X-ray fluorescence (μ-XRF) mapping. The extracellular thiol groups (SH) were first alkylated by iodoacetic acid forming Protein-SCH2COOH and then the P-SCH2COOH was marked by calcium ions forming P-SCH2COOCa. The STXM imaging and μ-XRF mapping of SH were based on analysis of SCH2COO-bonded Ca2+. The results indicated that the thiol group content of A. ferrooxidans grown on S0 is 3.88 times to that on Fe2+. Combined with selective labeling of SH by Ca2+, the STXM imaging and μ-XRF mapping provided an in situ and rapid analysis of differential expression of extracellular thiol groups.
•STXM imaging and m-XRF mapping were firstly used for thiol group analysis.•The membrane thiol groups were selectively labeled with Ca2+.•STXM image provided an in situ evaluation of extracellular thiol groups.•Micro-XRF mapping was rapid to determine the differential expression of thiol groups.•The -SH content of A. ferrooxidans grown on S0 was 3.88 times more to that on Fe2+.</description><subject>Acidithiobacillus ferrooxidans</subject><subject>Extracellular thiol group</subject><subject>Micro-XRF mapping</subject><subject>STXM imaging</subject><subject>Synchrotron radiation</subject><issn>0167-7012</issn><issn>1872-8359</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNp9kc9u1DAQxi1EJZbSJ-jFRySU1H82sXPgUFUsVCpCYlupN8uxJ-2skjjY2bL7VLwiTpczpxmNf99nzXyEXHJWcsbrq1054ABzKRiXJatLJtkbsuJaiULLqnlLVplShWJcvCPvU9oxxiu51ivyZ3sc3XMMcwwjjdajnTF3rU3g6fb-8Tu1o-2PCVNuPB3QxVA8_tzQwU4Tjk80dNRj10GEcUbbUzhMEVJaTPITHOZoHfT9vreRzs8YevoUw35KtD3Sa4cel2FrHWYm0ewTQzigt2NawN_ZZaQbEJ9ev9-yD-Sss32Ci3_1nDxsvtzffCvufny9vbm-KxzXXBROKu89iLrpZKtlrlK5rvK8aYVuWSOBu7XWUHHZclCNk1pppda1FooL4eQ5-XjynWL4tYc0mwHTsogdIeyT4bVQjapqKTIqT2g-TUoROjNFHGw8Gs7MEo_Zmdd4zBKPYbXJ8WTV55MK8hYvCNEkhzA68BjBzcYH_K_-L_IgnK8</recordid><startdate>201309</startdate><enddate>201309</enddate><creator>Xia, Jin-Lan</creator><creator>Liu, Hong-Chang</creator><creator>Nie, Zhen-Yuan</creator><creator>Peng, An-An</creator><creator>Zhen, Xiang-Jun</creator><creator>Yang, Yun</creator><creator>Zhang, Xiu-Li</creator><general>Elsevier B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>201309</creationdate><title>Synchrotron radiation based STXM analysis and micro-XRF mapping of differential expression of extracellular thiol groups by Acidithiobacillus ferrooxidans grown on Fe2+ and S0</title><author>Xia, Jin-Lan ; Liu, Hong-Chang ; Nie, Zhen-Yuan ; Peng, An-An ; Zhen, Xiang-Jun ; Yang, Yun ; Zhang, Xiu-Li</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1812-c37ddde269f3b8326937cf5d19b28b093e1c488e513b1e79c38787746827122c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Acidithiobacillus ferrooxidans</topic><topic>Extracellular thiol group</topic><topic>Micro-XRF mapping</topic><topic>STXM imaging</topic><topic>Synchrotron radiation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xia, Jin-Lan</creatorcontrib><creatorcontrib>Liu, Hong-Chang</creatorcontrib><creatorcontrib>Nie, Zhen-Yuan</creatorcontrib><creatorcontrib>Peng, An-An</creatorcontrib><creatorcontrib>Zhen, Xiang-Jun</creatorcontrib><creatorcontrib>Yang, Yun</creatorcontrib><creatorcontrib>Zhang, Xiu-Li</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of microbiological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xia, Jin-Lan</au><au>Liu, Hong-Chang</au><au>Nie, Zhen-Yuan</au><au>Peng, An-An</au><au>Zhen, Xiang-Jun</au><au>Yang, Yun</au><au>Zhang, Xiu-Li</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synchrotron radiation based STXM analysis and micro-XRF mapping of differential expression of extracellular thiol groups by Acidithiobacillus ferrooxidans grown on Fe2+ and S0</atitle><jtitle>Journal of microbiological methods</jtitle><date>2013-09</date><risdate>2013</risdate><volume>94</volume><issue>3</issue><spage>257</spage><epage>261</epage><pages>257-261</pages><issn>0167-7012</issn><eissn>1872-8359</eissn><abstract>The differential expression of extracellular thiol groups by Acidithiobacillus ferrooxidans grown on substrates Fe2+ and S0 was investigated by using synchrotron radiation based scanning transmission X-ray microscopy (STXM) imaging and microbeam X-ray fluorescence (μ-XRF) mapping. The extracellular thiol groups (SH) were first alkylated by iodoacetic acid forming Protein-SCH2COOH and then the P-SCH2COOH was marked by calcium ions forming P-SCH2COOCa. The STXM imaging and μ-XRF mapping of SH were based on analysis of SCH2COO-bonded Ca2+. The results indicated that the thiol group content of A. ferrooxidans grown on S0 is 3.88 times to that on Fe2+. Combined with selective labeling of SH by Ca2+, the STXM imaging and μ-XRF mapping provided an in situ and rapid analysis of differential expression of extracellular thiol groups.
•STXM imaging and m-XRF mapping were firstly used for thiol group analysis.•The membrane thiol groups were selectively labeled with Ca2+.•STXM image provided an in situ evaluation of extracellular thiol groups.•Micro-XRF mapping was rapid to determine the differential expression of thiol groups.•The -SH content of A. ferrooxidans grown on S0 was 3.88 times more to that on Fe2+.</abstract><pub>Elsevier B.V</pub><doi>10.1016/j.mimet.2013.06.030</doi><tpages>5</tpages></addata></record> |
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| subjects | Acidithiobacillus ferrooxidans Extracellular thiol group Micro-XRF mapping STXM imaging Synchrotron radiation |
| title | Synchrotron radiation based STXM analysis and micro-XRF mapping of differential expression of extracellular thiol groups by Acidithiobacillus ferrooxidans grown on Fe2+ and S0 |
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