In situ microscopy: A perspective for industrial bioethanol production monitoring
This work reviews the state-of-the-art in image-based in situ methods with regard to their potential use for fermentation of Saccharomyces cerevisiae in sugarcane wine. The integration of real time information from fermentation tanks in the control strategies has high potential to promote better fer...
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| Veröffentlicht in: | Journal of microbiological methods 2013-06, Vol.93 (3), p.224-232 |
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| creator | Belini, Valdinei Luís Wiedemann, Philipp Suhr, Hajo |
| description | This work reviews the state-of-the-art in image-based in situ methods with regard to their potential use for fermentation of Saccharomyces cerevisiae in sugarcane wine. The integration of real time information from fermentation tanks in the control strategies has high potential to promote better fermentative performance. While several image-based techniques for the measurement of cell concentration have been established, a reliable and consistent viability measurement still remains a challenging task. Reagent-free methods that estimate viability from information contained in micrograph images are reviewed. Nevertheless, the inherent complexity of the sugarcane syrup medium imposes extra challenges regarding its representation in microscopic images and their evaluation by real time image analysis.
•In situ microscopy as a potential monitoring tool for bioethanol production.•Review of reagent-free methods that estimate cell viability from micrographs.•Comparison of the characteristics of different in situ microscopy systems.•Optical sampling in situ microscopy provides large statistics. |
| doi_str_mv | 10.1016/j.mimet.2013.03.009 |
| format | Article |
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•In situ microscopy as a potential monitoring tool for bioethanol production.•Review of reagent-free methods that estimate cell viability from micrographs.•Comparison of the characteristics of different in situ microscopy systems.•Optical sampling in situ microscopy provides large statistics.</description><subject>Biotechnology - methods</subject><subject>cane syrup</subject><subject>Ethanol - metabolism</subject><subject>Ethanol - toxicity</subject><subject>ethanol production</subject><subject>Fermentation</subject><subject>image analysis</subject><subject>Image processing</subject><subject>Image Processing, Computer-Assisted - methods</subject><subject>In situ microscopy</subject><subject>Microbial Viability - drug effects</subject><subject>microscopy</subject><subject>Microscopy - methods</subject><subject>monitoring</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - cytology</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Saccharomyces cerevisiae - physiology</subject><subject>Saccharum - metabolism</subject><subject>Sugarcane fermentation</subject><subject>tanks</subject><subject>Viability</subject><subject>Vitaceae</subject><subject>wines</subject><subject>Yeast</subject><issn>0167-7012</issn><issn>1872-8359</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkd9r1TAUgIM43N30LxA0j3vp9SRNmkbwYQydg8EYc8-hSU5nLm1Tk3aw_97c3emjSA7k5Tu_vkPIewZbBqz5tNuOYcRly4HVWygB-hXZsFbxqq2lfk02hVKVAsaPyUnOOwAma9G-Ice8llwwKTbk9mqiOSwrHYNLMbs4P32m53TGlGd0S3hE2sdEw-TXvKTQDdSGiMvPbooDnVP0a4HiRMc4hSWmMD28JUd9N2R89_KfkvtvX39cfK-uby6vLs6vKydYs1QWe2m94BybuuNSaes0833bul5LKxWXDUdQPULrVXnOaqtE06CHRlqA-pScHeqWKX6tmBczhuxwGLoJ45oNa7jSqhbqP9BaaAHQgi5ofUD3NnLC3swpjF16MgzMXrvZmWftZq_dQInnrA8vDVY7ov-b88dzAT4egL6LpntIIZv7u1KhgbKJ4LAnvhwILM4eAyaTXcDJoQ-pHML4GP45wm-k5Z5r</recordid><startdate>20130601</startdate><enddate>20130601</enddate><creator>Belini, Valdinei Luís</creator><creator>Wiedemann, Philipp</creator><creator>Suhr, Hajo</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20130601</creationdate><title>In situ microscopy: A perspective for industrial bioethanol production monitoring</title><author>Belini, Valdinei Luís ; Wiedemann, Philipp ; Suhr, Hajo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c416t-bef5bd422e63a2579bc91df88cf95b572562e07fe08d7d7dcb9b7466ed065b003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Biotechnology - methods</topic><topic>cane syrup</topic><topic>Ethanol - metabolism</topic><topic>Ethanol - toxicity</topic><topic>ethanol production</topic><topic>Fermentation</topic><topic>image analysis</topic><topic>Image processing</topic><topic>Image Processing, Computer-Assisted - methods</topic><topic>In situ microscopy</topic><topic>Microbial Viability - drug effects</topic><topic>microscopy</topic><topic>Microscopy - methods</topic><topic>monitoring</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - cytology</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Saccharomyces cerevisiae - physiology</topic><topic>Saccharum - metabolism</topic><topic>Sugarcane fermentation</topic><topic>tanks</topic><topic>Viability</topic><topic>Vitaceae</topic><topic>wines</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Belini, Valdinei Luís</creatorcontrib><creatorcontrib>Wiedemann, Philipp</creatorcontrib><creatorcontrib>Suhr, Hajo</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of microbiological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Belini, Valdinei Luís</au><au>Wiedemann, Philipp</au><au>Suhr, Hajo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In situ microscopy: A perspective for industrial bioethanol production monitoring</atitle><jtitle>Journal of microbiological methods</jtitle><addtitle>J Microbiol Methods</addtitle><date>2013-06-01</date><risdate>2013</risdate><volume>93</volume><issue>3</issue><spage>224</spage><epage>232</epage><pages>224-232</pages><issn>0167-7012</issn><eissn>1872-8359</eissn><abstract>This work reviews the state-of-the-art in image-based in situ methods with regard to their potential use for fermentation of Saccharomyces cerevisiae in sugarcane wine. 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| subjects | Biotechnology - methods cane syrup Ethanol - metabolism Ethanol - toxicity ethanol production Fermentation image analysis Image processing Image Processing, Computer-Assisted - methods In situ microscopy Microbial Viability - drug effects microscopy Microscopy - methods monitoring Saccharomyces cerevisiae Saccharomyces cerevisiae - cytology Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae - physiology Saccharum - metabolism Sugarcane fermentation tanks Viability Vitaceae wines Yeast |
| title | In situ microscopy: A perspective for industrial bioethanol production monitoring |
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