Profiling celiac disease antibody repertoire
Abstract The aim of this study was to dissect the autoantibody response in celiac disease (CD) that remains largely unknown, with the goal of identifying the disease-specific autoantigenic protein pattern or the so called epitome. Sera from CD patients were used to select immunoreactive antigens fro...
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| Veröffentlicht in: | Clinical immunology (Orlando, Fla.) Fla.), 2013-07, Vol.148 (1), p.99-109 |
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| creator | D'Angelo, Sara Mignone, Flavio Deantonio, Cecilia Di Niro, Roberto Bordoni, Roberta Marzari, Roberto De Bellis, Gianluca Not, Tarcisio Ferrara, Fortunato Bradbury, Andrew Santoro, Claudio Sblattero, Daniele |
| description | Abstract The aim of this study was to dissect the autoantibody response in celiac disease (CD) that remains largely unknown, with the goal of identifying the disease-specific autoantigenic protein pattern or the so called epitome. Sera from CD patients were used to select immunoreactive antigens from a cDNA phage-display library. Candidate genes were identified, the corresponding proteins produced and their immunoreactivity validated with sera from CD patients and controls. Thirteen CD-specific antigens were identified and further validated by protein microarray. The specificity for 6 of these antigens was confirmed by ELISA. Furthermore we showed that this antibody response was not abolished on a gluten free diet and was not shared with other autoimmune diseases. These antigens appear to be CD specific and independent of gluten induction. The utility of this panel extends beyond its diagnostic value and it may drive the attention to new targets for unbiased screens in autoimmunity research. |
| doi_str_mv | 10.1016/j.clim.2013.04.009 |
| format | Article |
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Sera from CD patients were used to select immunoreactive antigens from a cDNA phage-display library. Candidate genes were identified, the corresponding proteins produced and their immunoreactivity validated with sera from CD patients and controls. Thirteen CD-specific antigens were identified and further validated by protein microarray. The specificity for 6 of these antigens was confirmed by ELISA. Furthermore we showed that this antibody response was not abolished on a gluten free diet and was not shared with other autoimmune diseases. These antigens appear to be CD specific and independent of gluten induction. The utility of this panel extends beyond its diagnostic value and it may drive the attention to new targets for unbiased screens in autoimmunity research.</description><identifier>ISSN: 1521-6616</identifier><identifier>EISSN: 1521-7035</identifier><identifier>DOI: 10.1016/j.clim.2013.04.009</identifier><identifier>PMID: 23685219</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adolescent ; Adult ; Allergy and Immunology ; Autoantibodies - blood ; Autoantibodies - immunology ; Autoantibody ; Autoantigen ; Autoantigens - genetics ; Autoantigens - immunology ; Celiac disease ; Celiac Disease - blood ; Celiac Disease - diagnosis ; Celiac Disease - genetics ; Celiac Disease - immunology ; Cell Surface Display Techniques ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Male ; Middle Aged ; Next generation sequencing ; Oligonucleotide Array Sequence Analysis ; ORF-display libraries ; Protein Array Analysis ; Protein microarray ; ROC Curve ; Young Adult</subject><ispartof>Clinical immunology (Orlando, Fla.), 2013-07, Vol.148 (1), p.99-109</ispartof><rights>Elsevier Inc.</rights><rights>2013 Elsevier Inc.</rights><rights>Copyright © 2013 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c444t-dcd3ca4d7d9bc8664c1cff74136c65d030f81bc7ecb838cd0d15c185d02b08143</citedby><cites>FETCH-LOGICAL-c444t-dcd3ca4d7d9bc8664c1cff74136c65d030f81bc7ecb838cd0d15c185d02b08143</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1521661613001125$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23685219$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>D'Angelo, Sara</creatorcontrib><creatorcontrib>Mignone, Flavio</creatorcontrib><creatorcontrib>Deantonio, Cecilia</creatorcontrib><creatorcontrib>Di Niro, Roberto</creatorcontrib><creatorcontrib>Bordoni, Roberta</creatorcontrib><creatorcontrib>Marzari, Roberto</creatorcontrib><creatorcontrib>De Bellis, Gianluca</creatorcontrib><creatorcontrib>Not, Tarcisio</creatorcontrib><creatorcontrib>Ferrara, Fortunato</creatorcontrib><creatorcontrib>Bradbury, Andrew</creatorcontrib><creatorcontrib>Santoro, Claudio</creatorcontrib><creatorcontrib>Sblattero, Daniele</creatorcontrib><title>Profiling celiac disease antibody repertoire</title><title>Clinical immunology (Orlando, Fla.)</title><addtitle>Clin Immunol</addtitle><description>Abstract The aim of this study was to dissect the autoantibody response in celiac disease (CD) that remains largely unknown, with the goal of identifying the disease-specific autoantigenic protein pattern or the so called epitome. Sera from CD patients were used to select immunoreactive antigens from a cDNA phage-display library. Candidate genes were identified, the corresponding proteins produced and their immunoreactivity validated with sera from CD patients and controls. Thirteen CD-specific antigens were identified and further validated by protein microarray. The specificity for 6 of these antigens was confirmed by ELISA. Furthermore we showed that this antibody response was not abolished on a gluten free diet and was not shared with other autoimmune diseases. These antigens appear to be CD specific and independent of gluten induction. The utility of this panel extends beyond its diagnostic value and it may drive the attention to new targets for unbiased screens in autoimmunity research.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Allergy and Immunology</subject><subject>Autoantibodies - blood</subject><subject>Autoantibodies - immunology</subject><subject>Autoantibody</subject><subject>Autoantigen</subject><subject>Autoantigens - genetics</subject><subject>Autoantigens - immunology</subject><subject>Celiac disease</subject><subject>Celiac Disease - blood</subject><subject>Celiac Disease - diagnosis</subject><subject>Celiac Disease - genetics</subject><subject>Celiac Disease - immunology</subject><subject>Cell Surface Display Techniques</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Female</subject><subject>Humans</subject><subject>Infant</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Next generation sequencing</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>ORF-display libraries</subject><subject>Protein Array Analysis</subject><subject>Protein microarray</subject><subject>ROC Curve</subject><subject>Young Adult</subject><issn>1521-6616</issn><issn>1521-7035</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1r3DAQhkVJab76B3Ioe8wh685IsmRDCYTQpoFAC03Pwh6Ng7Zeeyt5A_vvK7PbHnpIThqY530RzwhxgVAgoPm4KqgP60ICqgJ0AVC_ESdYSlxaUOXRYTYGzbE4TWkFAKWU5p04lspUeVefiKvvcexCH4anBXEfGlr4kLhJvGiGKbSj3y0ibzhOY4h8Lt52TZ_4_eE9Ez-_fH68_bp8-HZ3f3vzsCSt9bT05BU12ltft1QZowmp66xGZciUHhR0FbZkmdpKVeTBY0lY5Y1soUKtzsTlvncTx99bTpNbh5S_1zcDj9vk0EhbW1kr-TqqjCmtrWydUblHKY4pRe7cJoZ1E3cOwc1C3crNQt0s1IF2WWgOfTj0b9s1-3-RvwYz8GkPcBbyHDi6RIEHYp-F0eT8GF7uv_4vnpEhUNP_4h2n1biNQ1bt0CXpwP2YTzpfFBUAoizVH9eGmmo</recordid><startdate>20130701</startdate><enddate>20130701</enddate><creator>D'Angelo, Sara</creator><creator>Mignone, Flavio</creator><creator>Deantonio, Cecilia</creator><creator>Di Niro, Roberto</creator><creator>Bordoni, Roberta</creator><creator>Marzari, Roberto</creator><creator>De Bellis, Gianluca</creator><creator>Not, Tarcisio</creator><creator>Ferrara, Fortunato</creator><creator>Bradbury, Andrew</creator><creator>Santoro, Claudio</creator><creator>Sblattero, Daniele</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>20130701</creationdate><title>Profiling celiac disease antibody repertoire</title><author>D'Angelo, Sara ; 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| subjects | Adolescent Adult Allergy and Immunology Autoantibodies - blood Autoantibodies - immunology Autoantibody Autoantigen Autoantigens - genetics Autoantigens - immunology Celiac disease Celiac Disease - blood Celiac Disease - diagnosis Celiac Disease - genetics Celiac Disease - immunology Cell Surface Display Techniques Child Child, Preschool Female Humans Infant Male Middle Aged Next generation sequencing Oligonucleotide Array Sequence Analysis ORF-display libraries Protein Array Analysis Protein microarray ROC Curve Young Adult |
| title | Profiling celiac disease antibody repertoire |
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