Role of MoAND1-mediated nuclear positioning in morphogenesis and pathogenicity in the rice blast fungus, Magnaporthe oryzae
•MoAND1 is a putative cell cortex protein involved in nuclear positioning in M. oryzae.•Deletion of MoAND1 interferes with nuclear distribution.•MoAND1-mediated nuclear positioning is required for normal asexual development.•The ΔMoand1 is defective in conidial germination and appressorium formation...
Gespeichert in:
Veröffentlicht in: | Fungal genetics and biology 2014-08, Vol.69, p.43-51 |
---|---|
Hauptverfasser: | , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 51 |
---|---|
container_issue | |
container_start_page | 43 |
container_title | Fungal genetics and biology |
container_volume | 69 |
creator | Jeon, Junhyun Rho, Heekyoung Kim, Seongbeom Kim, Kyoung Su Lee, Yong-Hwan |
description | •MoAND1 is a putative cell cortex protein involved in nuclear positioning in M. oryzae.•Deletion of MoAND1 interferes with nuclear distribution.•MoAND1-mediated nuclear positioning is required for normal asexual development.•The ΔMoand1 is defective in conidial germination and appressorium formation.•MoAND1 is required not for invasive growth but for pre-penetration developments.
To cause disease on host plants, many phytopathogenic fungi undergo morphological transitions including development of reproductive structures as well as specialized infection structures called appressoria. Such morphological transitions display distinct nuclear dynamics. Here we report the developmental requirement of MoAND1-mediated nuclear positioning for pathogenesis of the rice blast fungus, Magnaporthe oryzae. The MoAND1 gene encodes a protein that shows high similarity to Num1 in Saccharomyces cerevisiae and ApsA in Aspergillus nidulans, both of which are cell cortex proteins involved in nuclear migration and positioning. Targeted deletion of MoAND1 did not affect radial growth of the fungus but impaired nuclear distribution along the hyphae, which is reminiscent of ApsA mutant. In contrast to the wild-type, which produces three to five spores in a sympodial manner on the conidiophore, only a single spore was borne on the conidiophore of ΔMoand1, resulting in ∼65% decrease in conidia production, compared to the wild-type. The mutant conidia displayed abnormalities in septation pattern and nuclear distribution, which were correlated with their inability to germinate. Spores of the mutant that did germinate were capable of differentiating appressoria but were defective in the execution of programmed nuclear migration and positioning during development. Furthermore, mutant appressoria were not fully functional, leading to delay in penetration of host plants. However, the ability of ΔMoand1 to grow inside host tissues was comparable to that of the wild-type. All these defects greatly decreased the virulence of the mutant. Taken together, our data suggest that there is a stringent but incomplete developmental requirement for proper migration and positioning of fungal nuclei mediated by MoAND1 during asexual reproduction and pre-penetration phase of fungal pathogenesis. |
doi_str_mv | 10.1016/j.fgb.2014.05.002 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1627963141</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1087184514000802</els_id><sourcerecordid>1627963141</sourcerecordid><originalsourceid>FETCH-LOGICAL-c429t-d7e1eadeeefe5c1eb35ebfd93e0e35ad2bdd4503d3a59d3cc39916eb444e64953</originalsourceid><addsrcrecordid>eNqFkU1v1DAQhi0EoqXwA7ggHzmQ4IntZC1OVfmUWpAQnC3HnqReZe1gO0gLf56ELRzhNKOZZ97DPIQ8BVYDg_blvh7Gvm4YiJrJmrHmHjkHptqKKd7d3_pdV8FOyDPyKOc9YwBSwENy1ohdJ0XTnJOfn-OENA70Jl5-fA3VAZ03BR0Ni53QJDrH7IuPwYeR-kAPMc23ccSA2WdqgqOzKb8H3vpy3JByizR5i7SfTC50WMK45Bf0xozBzDFt65iOPww-Jg8GM2V8clcvyNe3b75cva-uP737cHV5XVnRqFK5DgGNQ8QBpQXsucR-cIojQy6Na3rnhGTccSOV49ZypaDFXgiBrVCSX5Dnp9w5xW8L5qIPPlucJhMwLllD23Sq5SDg_6gUCrqOyw2FE2pTzDnhoOfkDyYdNTC96dF7verRmx7NpF71rDfP7uKXfv3034s_Plbg1QnA9R_fPSadrcdgVysJbdEu-n_E_wJIeqJS</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1549177351</pqid></control><display><type>article</type><title>Role of MoAND1-mediated nuclear positioning in morphogenesis and pathogenicity in the rice blast fungus, Magnaporthe oryzae</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Jeon, Junhyun ; Rho, Heekyoung ; Kim, Seongbeom ; Kim, Kyoung Su ; Lee, Yong-Hwan</creator><creatorcontrib>Jeon, Junhyun ; Rho, Heekyoung ; Kim, Seongbeom ; Kim, Kyoung Su ; Lee, Yong-Hwan</creatorcontrib><description>•MoAND1 is a putative cell cortex protein involved in nuclear positioning in M. oryzae.•Deletion of MoAND1 interferes with nuclear distribution.•MoAND1-mediated nuclear positioning is required for normal asexual development.•The ΔMoand1 is defective in conidial germination and appressorium formation.•MoAND1 is required not for invasive growth but for pre-penetration developments.
To cause disease on host plants, many phytopathogenic fungi undergo morphological transitions including development of reproductive structures as well as specialized infection structures called appressoria. Such morphological transitions display distinct nuclear dynamics. Here we report the developmental requirement of MoAND1-mediated nuclear positioning for pathogenesis of the rice blast fungus, Magnaporthe oryzae. The MoAND1 gene encodes a protein that shows high similarity to Num1 in Saccharomyces cerevisiae and ApsA in Aspergillus nidulans, both of which are cell cortex proteins involved in nuclear migration and positioning. Targeted deletion of MoAND1 did not affect radial growth of the fungus but impaired nuclear distribution along the hyphae, which is reminiscent of ApsA mutant. In contrast to the wild-type, which produces three to five spores in a sympodial manner on the conidiophore, only a single spore was borne on the conidiophore of ΔMoand1, resulting in ∼65% decrease in conidia production, compared to the wild-type. The mutant conidia displayed abnormalities in septation pattern and nuclear distribution, which were correlated with their inability to germinate. Spores of the mutant that did germinate were capable of differentiating appressoria but were defective in the execution of programmed nuclear migration and positioning during development. Furthermore, mutant appressoria were not fully functional, leading to delay in penetration of host plants. However, the ability of ΔMoand1 to grow inside host tissues was comparable to that of the wild-type. All these defects greatly decreased the virulence of the mutant. Taken together, our data suggest that there is a stringent but incomplete developmental requirement for proper migration and positioning of fungal nuclei mediated by MoAND1 during asexual reproduction and pre-penetration phase of fungal pathogenesis.</description><identifier>ISSN: 1087-1845</identifier><identifier>EISSN: 1096-0937</identifier><identifier>DOI: 10.1016/j.fgb.2014.05.002</identifier><identifier>PMID: 24875422</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Appressorium formation ; Asexual reproduction ; Aspergillus nidulans ; Aspergillus nidulans - genetics ; Cell Nucleus - metabolism ; Cytoskeletal Proteins - genetics ; Cytoskeletal Proteins - metabolism ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; Gene Deletion ; Magnaporthe - cytology ; Magnaporthe - growth & development ; Magnaporthe - metabolism ; Magnaporthe - pathogenicity ; MoAND1 ; Nuclear positioning ; Oryza - microbiology ; Oryza sativa ; Pathogenicity ; Plant Diseases - microbiology ; Rice blast ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Sequence Homology, Amino Acid ; Spores, Fungal - growth & development</subject><ispartof>Fungal genetics and biology, 2014-08, Vol.69, p.43-51</ispartof><rights>2014 The Authors</rights><rights>Copyright © 2014 The Authors. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c429t-d7e1eadeeefe5c1eb35ebfd93e0e35ad2bdd4503d3a59d3cc39916eb444e64953</citedby><cites>FETCH-LOGICAL-c429t-d7e1eadeeefe5c1eb35ebfd93e0e35ad2bdd4503d3a59d3cc39916eb444e64953</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.fgb.2014.05.002$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24875422$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jeon, Junhyun</creatorcontrib><creatorcontrib>Rho, Heekyoung</creatorcontrib><creatorcontrib>Kim, Seongbeom</creatorcontrib><creatorcontrib>Kim, Kyoung Su</creatorcontrib><creatorcontrib>Lee, Yong-Hwan</creatorcontrib><title>Role of MoAND1-mediated nuclear positioning in morphogenesis and pathogenicity in the rice blast fungus, Magnaporthe oryzae</title><title>Fungal genetics and biology</title><addtitle>Fungal Genet Biol</addtitle><description>•MoAND1 is a putative cell cortex protein involved in nuclear positioning in M. oryzae.•Deletion of MoAND1 interferes with nuclear distribution.•MoAND1-mediated nuclear positioning is required for normal asexual development.•The ΔMoand1 is defective in conidial germination and appressorium formation.•MoAND1 is required not for invasive growth but for pre-penetration developments.
To cause disease on host plants, many phytopathogenic fungi undergo morphological transitions including development of reproductive structures as well as specialized infection structures called appressoria. Such morphological transitions display distinct nuclear dynamics. Here we report the developmental requirement of MoAND1-mediated nuclear positioning for pathogenesis of the rice blast fungus, Magnaporthe oryzae. The MoAND1 gene encodes a protein that shows high similarity to Num1 in Saccharomyces cerevisiae and ApsA in Aspergillus nidulans, both of which are cell cortex proteins involved in nuclear migration and positioning. Targeted deletion of MoAND1 did not affect radial growth of the fungus but impaired nuclear distribution along the hyphae, which is reminiscent of ApsA mutant. In contrast to the wild-type, which produces three to five spores in a sympodial manner on the conidiophore, only a single spore was borne on the conidiophore of ΔMoand1, resulting in ∼65% decrease in conidia production, compared to the wild-type. The mutant conidia displayed abnormalities in septation pattern and nuclear distribution, which were correlated with their inability to germinate. Spores of the mutant that did germinate were capable of differentiating appressoria but were defective in the execution of programmed nuclear migration and positioning during development. Furthermore, mutant appressoria were not fully functional, leading to delay in penetration of host plants. However, the ability of ΔMoand1 to grow inside host tissues was comparable to that of the wild-type. All these defects greatly decreased the virulence of the mutant. Taken together, our data suggest that there is a stringent but incomplete developmental requirement for proper migration and positioning of fungal nuclei mediated by MoAND1 during asexual reproduction and pre-penetration phase of fungal pathogenesis.</description><subject>Appressorium formation</subject><subject>Asexual reproduction</subject><subject>Aspergillus nidulans</subject><subject>Aspergillus nidulans - genetics</subject><subject>Cell Nucleus - metabolism</subject><subject>Cytoskeletal Proteins - genetics</subject><subject>Cytoskeletal Proteins - metabolism</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>Gene Deletion</subject><subject>Magnaporthe - cytology</subject><subject>Magnaporthe - growth & development</subject><subject>Magnaporthe - metabolism</subject><subject>Magnaporthe - pathogenicity</subject><subject>MoAND1</subject><subject>Nuclear positioning</subject><subject>Oryza - microbiology</subject><subject>Oryza sativa</subject><subject>Pathogenicity</subject><subject>Plant Diseases - microbiology</subject><subject>Rice blast</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Sequence Homology, Amino Acid</subject><subject>Spores, Fungal - growth & development</subject><issn>1087-1845</issn><issn>1096-0937</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EoqXwA7ggHzmQ4IntZC1OVfmUWpAQnC3HnqReZe1gO0gLf56ELRzhNKOZZ97DPIQ8BVYDg_blvh7Gvm4YiJrJmrHmHjkHptqKKd7d3_pdV8FOyDPyKOc9YwBSwENy1ohdJ0XTnJOfn-OENA70Jl5-fA3VAZ03BR0Ni53QJDrH7IuPwYeR-kAPMc23ccSA2WdqgqOzKb8H3vpy3JByizR5i7SfTC50WMK45Bf0xozBzDFt65iOPww-Jg8GM2V8clcvyNe3b75cva-uP737cHV5XVnRqFK5DgGNQ8QBpQXsucR-cIojQy6Na3rnhGTccSOV49ZypaDFXgiBrVCSX5Dnp9w5xW8L5qIPPlucJhMwLllD23Sq5SDg_6gUCrqOyw2FE2pTzDnhoOfkDyYdNTC96dF7verRmx7NpF71rDfP7uKXfv3034s_Plbg1QnA9R_fPSadrcdgVysJbdEu-n_E_wJIeqJS</recordid><startdate>20140801</startdate><enddate>20140801</enddate><creator>Jeon, Junhyun</creator><creator>Rho, Heekyoung</creator><creator>Kim, Seongbeom</creator><creator>Kim, Kyoung Su</creator><creator>Lee, Yong-Hwan</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20140801</creationdate><title>Role of MoAND1-mediated nuclear positioning in morphogenesis and pathogenicity in the rice blast fungus, Magnaporthe oryzae</title><author>Jeon, Junhyun ; Rho, Heekyoung ; Kim, Seongbeom ; Kim, Kyoung Su ; Lee, Yong-Hwan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c429t-d7e1eadeeefe5c1eb35ebfd93e0e35ad2bdd4503d3a59d3cc39916eb444e64953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Appressorium formation</topic><topic>Asexual reproduction</topic><topic>Aspergillus nidulans</topic><topic>Aspergillus nidulans - genetics</topic><topic>Cell Nucleus - metabolism</topic><topic>Cytoskeletal Proteins - genetics</topic><topic>Cytoskeletal Proteins - metabolism</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>Gene Deletion</topic><topic>Magnaporthe - cytology</topic><topic>Magnaporthe - growth & development</topic><topic>Magnaporthe - metabolism</topic><topic>Magnaporthe - pathogenicity</topic><topic>MoAND1</topic><topic>Nuclear positioning</topic><topic>Oryza - microbiology</topic><topic>Oryza sativa</topic><topic>Pathogenicity</topic><topic>Plant Diseases - microbiology</topic><topic>Rice blast</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Sequence Homology, Amino Acid</topic><topic>Spores, Fungal - growth & development</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jeon, Junhyun</creatorcontrib><creatorcontrib>Rho, Heekyoung</creatorcontrib><creatorcontrib>Kim, Seongbeom</creatorcontrib><creatorcontrib>Kim, Kyoung Su</creatorcontrib><creatorcontrib>Lee, Yong-Hwan</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Fungal genetics and biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jeon, Junhyun</au><au>Rho, Heekyoung</au><au>Kim, Seongbeom</au><au>Kim, Kyoung Su</au><au>Lee, Yong-Hwan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of MoAND1-mediated nuclear positioning in morphogenesis and pathogenicity in the rice blast fungus, Magnaporthe oryzae</atitle><jtitle>Fungal genetics and biology</jtitle><addtitle>Fungal Genet Biol</addtitle><date>2014-08-01</date><risdate>2014</risdate><volume>69</volume><spage>43</spage><epage>51</epage><pages>43-51</pages><issn>1087-1845</issn><eissn>1096-0937</eissn><abstract>•MoAND1 is a putative cell cortex protein involved in nuclear positioning in M. oryzae.•Deletion of MoAND1 interferes with nuclear distribution.•MoAND1-mediated nuclear positioning is required for normal asexual development.•The ΔMoand1 is defective in conidial germination and appressorium formation.•MoAND1 is required not for invasive growth but for pre-penetration developments.
To cause disease on host plants, many phytopathogenic fungi undergo morphological transitions including development of reproductive structures as well as specialized infection structures called appressoria. Such morphological transitions display distinct nuclear dynamics. Here we report the developmental requirement of MoAND1-mediated nuclear positioning for pathogenesis of the rice blast fungus, Magnaporthe oryzae. The MoAND1 gene encodes a protein that shows high similarity to Num1 in Saccharomyces cerevisiae and ApsA in Aspergillus nidulans, both of which are cell cortex proteins involved in nuclear migration and positioning. Targeted deletion of MoAND1 did not affect radial growth of the fungus but impaired nuclear distribution along the hyphae, which is reminiscent of ApsA mutant. In contrast to the wild-type, which produces three to five spores in a sympodial manner on the conidiophore, only a single spore was borne on the conidiophore of ΔMoand1, resulting in ∼65% decrease in conidia production, compared to the wild-type. The mutant conidia displayed abnormalities in septation pattern and nuclear distribution, which were correlated with their inability to germinate. Spores of the mutant that did germinate were capable of differentiating appressoria but were defective in the execution of programmed nuclear migration and positioning during development. Furthermore, mutant appressoria were not fully functional, leading to delay in penetration of host plants. However, the ability of ΔMoand1 to grow inside host tissues was comparable to that of the wild-type. All these defects greatly decreased the virulence of the mutant. Taken together, our data suggest that there is a stringent but incomplete developmental requirement for proper migration and positioning of fungal nuclei mediated by MoAND1 during asexual reproduction and pre-penetration phase of fungal pathogenesis.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>24875422</pmid><doi>10.1016/j.fgb.2014.05.002</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1087-1845 |
ispartof | Fungal genetics and biology, 2014-08, Vol.69, p.43-51 |
issn | 1087-1845 1096-0937 |
language | eng |
recordid | cdi_proquest_miscellaneous_1627963141 |
source | MEDLINE; Elsevier ScienceDirect Journals Complete |
subjects | Appressorium formation Asexual reproduction Aspergillus nidulans Aspergillus nidulans - genetics Cell Nucleus - metabolism Cytoskeletal Proteins - genetics Cytoskeletal Proteins - metabolism Fungal Proteins - genetics Fungal Proteins - metabolism Gene Deletion Magnaporthe - cytology Magnaporthe - growth & development Magnaporthe - metabolism Magnaporthe - pathogenicity MoAND1 Nuclear positioning Oryza - microbiology Oryza sativa Pathogenicity Plant Diseases - microbiology Rice blast Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Sequence Homology, Amino Acid Spores, Fungal - growth & development |
title | Role of MoAND1-mediated nuclear positioning in morphogenesis and pathogenicity in the rice blast fungus, Magnaporthe oryzae |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T13%3A42%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Role%20of%20MoAND1-mediated%20nuclear%20positioning%20in%20morphogenesis%20and%20pathogenicity%20in%20the%20rice%20blast%20fungus,%20Magnaporthe%20oryzae&rft.jtitle=Fungal%20genetics%20and%20biology&rft.au=Jeon,%20Junhyun&rft.date=2014-08-01&rft.volume=69&rft.spage=43&rft.epage=51&rft.pages=43-51&rft.issn=1087-1845&rft.eissn=1096-0937&rft_id=info:doi/10.1016/j.fgb.2014.05.002&rft_dat=%3Cproquest_cross%3E1627963141%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1549177351&rft_id=info:pmid/24875422&rft_els_id=S1087184514000802&rfr_iscdi=true |