In vivo imaging of mitochondria in intact zebrafish larvae
Visualizing neuronal mitochondria in a living, intact mammalian organism is a challenge that can be overcome in zebrafish larvae, which are highly accessible for optical imaging and genetic manipulation. Here, we detail an approach to visualize neuronal mitochondria in sensory Rohon-Beard axons, whi...
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| Veröffentlicht in: | Methods in enzymology 2014, Vol.547, p.151-164 |
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| container_title | Methods in enzymology |
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| creator | Paquet, Dominik Plucińska, Gabriela Misgeld, Thomas |
| description | Visualizing neuronal mitochondria in a living, intact mammalian organism is a challenge that can be overcome in zebrafish larvae, which are highly accessible for optical imaging and genetic manipulation. Here, we detail an approach to visualize neuronal mitochondria in sensory Rohon-Beard axons, which allows quantitatively measuring mitochondrial shape, dynamics, and transport in vivo. This provides a useful assay for basic studies exploring the behavior of neuronal mitochondria in their natural habitat, for revealing the influence that disease-related alterations have on this behavior and for testing pharmacological compounds and genetic manipulations that might ameliorate disease-related mitochondrial phenotypes in neurons. |
| doi_str_mv | 10.1016/B978-0-12-801415-8.00009-6 |
| format | Article |
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| ispartof | Methods in enzymology, 2014, Vol.547, p.151-164 |
| issn | 1557-7988 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Animals, Genetically Modified Automatic Data Processing Axonal Transport Axons Bacterial Proteins - genetics Bacterial Proteins - metabolism Embryo, Nonmammalian - cytology Image Processing, Computer-Assisted Larva - cytology Luminescent Proteins - genetics Luminescent Proteins - metabolism Microscopy, Fluorescence - instrumentation Microscopy, Fluorescence - methods Mitochondria - physiology Sensory Receptor Cells - cytology Zebrafish - embryology Zebrafish - genetics |
| title | In vivo imaging of mitochondria in intact zebrafish larvae |
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