Functional co-expression of human oxidoreductase and cytochrome P450 1A1 in Saccharomyces cerevisiae results in increased erod activity

A cDNA coding for human oxidoreductase (NADPH-cytochrome P450 reductase) was expressed in S. cerevisiae on a high copy plasmid under control of a constitutive promoter. Microsomes from a transformed strain lacking endogenous oxidoreductase exhibited cytochrome c reductase activity. An apparent K m o...

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Veröffentlicht in:	Biochemical and biophysical research communications 1992-06, Vol.185 (2), p.641-647
Hauptverfasser:	Eugster, Hans-Pietro, Bärtsch, Stephan, Würgler, Friedrich E., Sengstag, Christian
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Base Sequence Biological and medical sciences Cloning, Molecular Cytochrome P-450 CYP1A1 Cytochrome P-450 Enzyme System - metabolism Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Genetic Complementation Test Humans Kinetics Molecular Sequence Data Mutagenesis, Insertional NADPH-Ferrihemoprotein Reductase - metabolism Oligodeoxyribonucleotides - chemistry Oxidoreductases Oxidoreductases - metabolism Recombinant Proteins Saccharomyces cerevisiae Species Specificity Transfection
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