The paraventricular nucleus of the hypothalamus influences respiratory timing and activity in the rat
In this study we sought to determine the role of the paraventricular nucleus of the hypothalamus (PVN) in modulating respiratory output. Experiments were performed in urethane anesthetized, vagotomized and mechanically ventilated Wistar rats. Electromyographic activity of the diaphragm (DEMG) was re...
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| Veröffentlicht in: | Neuroscience letters 1997-08, Vol.232 (2), p.63-66 |
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| creator | Yeh, Edwin R Erokwu, Bernadette LaManna, Joseph C Haxhiu, Musa A |
| description | In this study we sought to determine the role of the paraventricular nucleus of the hypothalamus (PVN) in modulating respiratory output. Experiments were performed in urethane anesthetized, vagotomized and mechanically ventilated Wistar rats. Electromyographic activity of the diaphragm (DEMG) was recorded and used to define the respiratory effects of PVN stimulation. The ventilation rate and volume were pre-adjusted so that baseline activity was 30% of the activity observed upon addition of 7% CO2 in O2. Microinjection of l-glutamate (4 nmol, 100 nl) into the PVN produced an increase in peak DEMG, and an increase in frequency of DEMG discharge. Changes in respiratory timing were mainly due to shortening of expiratory time (0.66±0.06 s vs. 0.90±0.10 s; mean±SEM; P005). The rate of rise of DEMG increased by 101±28% from the baseline (P |
| doi_str_mv | 10.1016/S0304-3940(97)00579-X |
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Experiments were performed in urethane anesthetized, vagotomized and mechanically ventilated Wistar rats. Electromyographic activity of the diaphragm (DEMG) was recorded and used to define the respiratory effects of PVN stimulation. The ventilation rate and volume were pre-adjusted so that baseline activity was 30% of the activity observed upon addition of 7% CO2 in O2. Microinjection of l-glutamate (4 nmol, 100 nl) into the PVN produced an increase in peak DEMG, and an increase in frequency of DEMG discharge. Changes in respiratory timing were mainly due to shortening of expiratory time (0.66±0.06 s vs. 0.90±0.10 s; mean±SEM; P<0.05), while inspiratory time was less affected (0.48±0.04 vs. 0.51±0.04 s; P>005). The rate of rise of DEMG increased by 101±28% from the baseline (P<0.05). In addition, neuroanatomical tracing studies suggest the presence of direct connection between PVN and phrenic motoneurons. The results indicate that PVN neurons participate in regulation of breathing activity and in coordination of cardiovascular and respiratory functions.</description><identifier>ISSN: 0304-3940</identifier><identifier>EISSN: 1872-7972</identifier><identifier>DOI: 10.1016/S0304-3940(97)00579-X</identifier><identifier>PMID: 9302087</identifier><identifier>CODEN: NELED5</identifier><language>eng</language><publisher>Shannon: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Blood Pressure - drug effects ; Cardiorespiratory control. Arterial mecano- and chemoreceptor ; Cholera Toxin - analysis ; Control of breathing ; Diaphragm - drug effects ; Diaphragm - physiology ; Electromyographic activity of the diaphragm ; Electromyography ; Expiratory time ; Fundamental and applied biological sciences. Psychology ; Glutamic Acid - pharmacology ; Hypothalamus ; Inspiratory time ; Paraventricular Hypothalamic Nucleus - chemistry ; Paraventricular Hypothalamic Nucleus - drug effects ; Paraventricular Hypothalamic Nucleus - physiology ; Paraventricular nucleus ; Phrenic Nerve - drug effects ; Rat ; Rats ; Rats, Wistar ; Respiration ; Respiratory Mechanics - drug effects ; Respiratory Mechanics - physiology ; Respiratory timing ; Retrograde tracing ; Vagotomy ; Vertebrates: respiratory system</subject><ispartof>Neuroscience letters, 1997-08, Vol.232 (2), p.63-66</ispartof><rights>1997 Elsevier Science Ireland Ltd</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c538t-700b0ecbd1ad804b922325104b4fd2ab4c7faf0fb3cd8f88251f9fc6a26843bf3</citedby><cites>FETCH-LOGICAL-c538t-700b0ecbd1ad804b922325104b4fd2ab4c7faf0fb3cd8f88251f9fc6a26843bf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0304-3940(97)00579-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2819909$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9302087$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yeh, Edwin R</creatorcontrib><creatorcontrib>Erokwu, Bernadette</creatorcontrib><creatorcontrib>LaManna, Joseph C</creatorcontrib><creatorcontrib>Haxhiu, Musa A</creatorcontrib><title>The paraventricular nucleus of the hypothalamus influences respiratory timing and activity in the rat</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>In this study we sought to determine the role of the paraventricular nucleus of the hypothalamus (PVN) in modulating respiratory output. Experiments were performed in urethane anesthetized, vagotomized and mechanically ventilated Wistar rats. Electromyographic activity of the diaphragm (DEMG) was recorded and used to define the respiratory effects of PVN stimulation. The ventilation rate and volume were pre-adjusted so that baseline activity was 30% of the activity observed upon addition of 7% CO2 in O2. Microinjection of l-glutamate (4 nmol, 100 nl) into the PVN produced an increase in peak DEMG, and an increase in frequency of DEMG discharge. Changes in respiratory timing were mainly due to shortening of expiratory time (0.66±0.06 s vs. 0.90±0.10 s; mean±SEM; P<0.05), while inspiratory time was less affected (0.48±0.04 vs. 0.51±0.04 s; P>005). The rate of rise of DEMG increased by 101±28% from the baseline (P<0.05). In addition, neuroanatomical tracing studies suggest the presence of direct connection between PVN and phrenic motoneurons. The results indicate that PVN neurons participate in regulation of breathing activity and in coordination of cardiovascular and respiratory functions.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blood Pressure - drug effects</subject><subject>Cardiorespiratory control. Arterial mecano- and chemoreceptor</subject><subject>Cholera Toxin - analysis</subject><subject>Control of breathing</subject><subject>Diaphragm - drug effects</subject><subject>Diaphragm - physiology</subject><subject>Electromyographic activity of the diaphragm</subject><subject>Electromyography</subject><subject>Expiratory time</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glutamic Acid - pharmacology</subject><subject>Hypothalamus</subject><subject>Inspiratory time</subject><subject>Paraventricular Hypothalamic Nucleus - chemistry</subject><subject>Paraventricular Hypothalamic Nucleus - drug effects</subject><subject>Paraventricular Hypothalamic Nucleus - physiology</subject><subject>Paraventricular nucleus</subject><subject>Phrenic Nerve - drug effects</subject><subject>Rat</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Respiration</subject><subject>Respiratory Mechanics - drug effects</subject><subject>Respiratory Mechanics - physiology</subject><subject>Respiratory timing</subject><subject>Retrograde tracing</subject><subject>Vagotomy</subject><subject>Vertebrates: respiratory system</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEuLFDEURoMoY8_oTxiohYizKL1JPZKsRAZfMODCEWYXbqVu7Ei9TFIN_e9NP-itq1zyne8mHMZuObznwNsPP6GCuqx0De-0vANopC6fnrENV1KUUkvxnG0uyEt2HeMfyBRv6it2pSsQoOSG0eOWigUD7mhKwdt1wFBMqx1ojcXsipTj7X6Z0xYHHPOdn9yw0mQpFoHi4gOmOeyL5Ec__S5w6gu0ye982mf0WM_EK_bC4RDp9fm8Yb--fH68_1Y-_Pj6_f7TQ2mbSqVSAnRAtus59grqTgtRiYbnqXa9wK620qED11W2V06pnDntbIuiVXXVueqGvT3tXcL8d6WYzOijpWHAieY1Gt6KFkCJDDYn0IY5xkDOLMGPGPaGgznoNUe95uDOaGmOes1T7t2eH1i7kfpL6-wz52_OOUaLgws4WR8vmFBca9AZ-3jCKMvYeQomWn-Q2vtANpl-9v_5yD_GSpl5</recordid><startdate>19970829</startdate><enddate>19970829</enddate><creator>Yeh, Edwin R</creator><creator>Erokwu, Bernadette</creator><creator>LaManna, Joseph C</creator><creator>Haxhiu, Musa A</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>19970829</creationdate><title>The paraventricular nucleus of the hypothalamus influences respiratory timing and activity in the rat</title><author>Yeh, Edwin R ; Erokwu, Bernadette ; LaManna, Joseph C ; Haxhiu, Musa A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c538t-700b0ecbd1ad804b922325104b4fd2ab4c7faf0fb3cd8f88251f9fc6a26843bf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blood Pressure - drug effects</topic><topic>Cardiorespiratory control. Arterial mecano- and chemoreceptor</topic><topic>Cholera Toxin - analysis</topic><topic>Control of breathing</topic><topic>Diaphragm - drug effects</topic><topic>Diaphragm - physiology</topic><topic>Electromyographic activity of the diaphragm</topic><topic>Electromyography</topic><topic>Expiratory time</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glutamic Acid - pharmacology</topic><topic>Hypothalamus</topic><topic>Inspiratory time</topic><topic>Paraventricular Hypothalamic Nucleus - chemistry</topic><topic>Paraventricular Hypothalamic Nucleus - drug effects</topic><topic>Paraventricular Hypothalamic Nucleus - physiology</topic><topic>Paraventricular nucleus</topic><topic>Phrenic Nerve - drug effects</topic><topic>Rat</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Respiration</topic><topic>Respiratory Mechanics - drug effects</topic><topic>Respiratory Mechanics - physiology</topic><topic>Respiratory timing</topic><topic>Retrograde tracing</topic><topic>Vagotomy</topic><topic>Vertebrates: respiratory system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yeh, Edwin R</creatorcontrib><creatorcontrib>Erokwu, Bernadette</creatorcontrib><creatorcontrib>LaManna, Joseph C</creatorcontrib><creatorcontrib>Haxhiu, Musa A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yeh, Edwin R</au><au>Erokwu, Bernadette</au><au>LaManna, Joseph C</au><au>Haxhiu, Musa A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The paraventricular nucleus of the hypothalamus influences respiratory timing and activity in the rat</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>1997-08-29</date><risdate>1997</risdate><volume>232</volume><issue>2</issue><spage>63</spage><epage>66</epage><pages>63-66</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>In this study we sought to determine the role of the paraventricular nucleus of the hypothalamus (PVN) in modulating respiratory output. Experiments were performed in urethane anesthetized, vagotomized and mechanically ventilated Wistar rats. Electromyographic activity of the diaphragm (DEMG) was recorded and used to define the respiratory effects of PVN stimulation. The ventilation rate and volume were pre-adjusted so that baseline activity was 30% of the activity observed upon addition of 7% CO2 in O2. Microinjection of l-glutamate (4 nmol, 100 nl) into the PVN produced an increase in peak DEMG, and an increase in frequency of DEMG discharge. Changes in respiratory timing were mainly due to shortening of expiratory time (0.66±0.06 s vs. 0.90±0.10 s; mean±SEM; P<0.05), while inspiratory time was less affected (0.48±0.04 vs. 0.51±0.04 s; P>005). The rate of rise of DEMG increased by 101±28% from the baseline (P<0.05). In addition, neuroanatomical tracing studies suggest the presence of direct connection between PVN and phrenic motoneurons. The results indicate that PVN neurons participate in regulation of breathing activity and in coordination of cardiovascular and respiratory functions.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>9302087</pmid><doi>10.1016/S0304-3940(97)00579-X</doi><tpages>4</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Blood Pressure - drug effects Cardiorespiratory control. Arterial mecano- and chemoreceptor Cholera Toxin - analysis Control of breathing Diaphragm - drug effects Diaphragm - physiology Electromyographic activity of the diaphragm Electromyography Expiratory time Fundamental and applied biological sciences. Psychology Glutamic Acid - pharmacology Hypothalamus Inspiratory time Paraventricular Hypothalamic Nucleus - chemistry Paraventricular Hypothalamic Nucleus - drug effects Paraventricular Hypothalamic Nucleus - physiology Paraventricular nucleus Phrenic Nerve - drug effects Rat Rats Rats, Wistar Respiration Respiratory Mechanics - drug effects Respiratory Mechanics - physiology Respiratory timing Retrograde tracing Vagotomy Vertebrates: respiratory system |
| title | The paraventricular nucleus of the hypothalamus influences respiratory timing and activity in the rat |
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