Rapid amplification of cDNA ends (RACE) improves the PCR-based isolation of immunoglobulin variable region genes from murine and human lymphoma cells and cell lines

The isolation of rearranged immunoglobulin (Ig) variable region (V) genes is usually performed by PCR with consensus primers binding to conserved regions within the V sequences. However, the isolation of Ig genes by this method is hampered in 15-35% by technical difficulties, mostly mismatches of ol...

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Veröffentlicht in:	Leukemia 1997-10, Vol.11 (10), p.1787-1792
Hauptverfasser:	DOENECKE, A, WINNACKER, E.-L, HALLEK, M
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Amplification Animals B-cell lymphoma Base Sequence Biological and medical sciences Chains Chronic lymphocytic leukemia Cloning Conserved sequence Constant region Deoxyribonucleic acid DNA DNA, Neoplasm - isolation & purification Gene Rearrangement Gene sequencing General aspects Genes Genes, Immunoglobulin Hematologic and hematopoietic diseases Humans Immunoglobulin Variable Region - genetics Immunoglobulins Leukemia Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis Lymphatic leukemia Lymphoma Lymphoma, B-Cell - genetics Medical sciences Molecular Sequence Data Multiple Myeloma - genetics Myeloma Nucleic Acid Amplification Techniques Nucleotide sequence Oligonucleotides Polymerase chain reaction Polymerase Chain Reaction - methods Primers Tumor cell lines Tumor Cells, Cultured Tumors Variable region
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