Effects of the Isoquinolinesulfonamide H-8 on Fundulus heteroclitus Ovarian Follicles: Role of Cyclic Nucleotide-Dependent Protein Kinases on Steroidogenesis and Oocyte Maturation In Vitro
The possible role of cyclic nucleotide-dependent protein kinases in mediating the stimulatory actions of Fundulus heteroclitus pituitary extract (FPE) during ovarian steroidogenesis and oocyte maturation in vitro was investigated. Follicle-enclosed oocytes were cultured in the presence of FPE and/or...
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| Veröffentlicht in: | Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology Comparative pharmacology and toxicology, 1997-05, Vol.117 (1), p.75-81 |
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| creator | Cerdà, J Petrino, T.R Landin, A.M Lin, Y-W.P |
| description | The possible role of cyclic nucleotide-dependent protein kinases in mediating the stimulatory actions of
Fundulus heteroclitus pituitary extract (FPE) during ovarian steroidogenesis and oocyte maturation
in vitro was investigated. Follicle-enclosed oocytes were cultured in the presence of FPE and/or N-[2-(Methylamino)ethyl]-5-isoquinolinesulfonamide (H-8), a compound that inhibits protein kinase A (PKA) and cGMP-dependent protein kinase. H-8 alone (0.1–1 mM) promoted oocyte germinal vesicle breakdown (GVBD) in a dose-dependent manner. However, the process of GVBD initiated by H-8 was much slower than that triggered by 17
α,20
β-dihydroxy-4-pregnen-3-one (17,20
βP), the natural inducer of oocyte maturation in
F. heteroclitus. Treatment with H-8 also increased 17,20
βP production by the follicles and the accumulation of this steroid in the media was much slower than that initiated by FPE. However, in contrast to the FPE action on the oocyte, which is mediated by 17,20
βP, the stimulatory action of H-8 on GVBD appears to be independent of follicular steroid production, since aminoglutethimide (AGI), an inhibitor of steroidogenesis, did not-block H-8-induced GVBD while inhibiting H-8-induced 17,20
βP production. Moreover, addition of H-8 to FPE-treated follicles significantly reduced 17,20
βP secretion and the percentage of GVBD. These results provide further support for the involvement of PKA in the mechanism by which FPE stimulates ovarian steroidogenesis in
F. heteroclitus. Furthermore, the fact that H-8 alone increased 17,20
βP levels may imply that basal follicular production of this steroid could be induced by inactivation of cyclic nucleotide-dependent protein kinases. Data also indicate that inhibition of PKA and/or c-GMP-dependent protein kinase in the oocyte may be involved in the mechanism leading to resumption of meiosis in this species. |
| doi_str_mv | 10.1016/S0742-8413(96)00236-8 |
| format | Article |
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Fundulus heteroclitus pituitary extract (FPE) during ovarian steroidogenesis and oocyte maturation
in vitro was investigated. Follicle-enclosed oocytes were cultured in the presence of FPE and/or N-[2-(Methylamino)ethyl]-5-isoquinolinesulfonamide (H-8), a compound that inhibits protein kinase A (PKA) and cGMP-dependent protein kinase. H-8 alone (0.1–1 mM) promoted oocyte germinal vesicle breakdown (GVBD) in a dose-dependent manner. However, the process of GVBD initiated by H-8 was much slower than that triggered by 17
α,20
β-dihydroxy-4-pregnen-3-one (17,20
βP), the natural inducer of oocyte maturation in
F. heteroclitus. Treatment with H-8 also increased 17,20
βP production by the follicles and the accumulation of this steroid in the media was much slower than that initiated by FPE. However, in contrast to the FPE action on the oocyte, which is mediated by 17,20
βP, the stimulatory action of H-8 on GVBD appears to be independent of follicular steroid production, since aminoglutethimide (AGI), an inhibitor of steroidogenesis, did not-block H-8-induced GVBD while inhibiting H-8-induced 17,20
βP production. Moreover, addition of H-8 to FPE-treated follicles significantly reduced 17,20
βP secretion and the percentage of GVBD. These results provide further support for the involvement of PKA in the mechanism by which FPE stimulates ovarian steroidogenesis in
F. heteroclitus. Furthermore, the fact that H-8 alone increased 17,20
βP levels may imply that basal follicular production of this steroid could be induced by inactivation of cyclic nucleotide-dependent protein kinases. Data also indicate that inhibition of PKA and/or c-GMP-dependent protein kinase in the oocyte may be involved in the mechanism leading to resumption of meiosis in this species.</description><identifier>ISSN: 0742-8413</identifier><identifier>ISSN: 1367-8280</identifier><identifier>DOI: 10.1016/S0742-8413(96)00236-8</identifier><identifier>PMID: 9185330</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Brackish ; cGMP-dependent protein kinase ; Cyclic AMP-Dependent Protein Kinases - adverse effects ; Cyclic GMP-Dependent Protein Kinases - physiology ; Female ; Fishes ; Fundulus heteroclitus ; gonadotropin ; Isoquinolines - antagonists & inhibitors ; Isoquinolines - pharmacology ; isoquinolinesulfonamide H-8 ; Killifishes - metabolism ; Marine ; oocyte maturation ; Oocytes - drug effects ; Oocytes - enzymology ; Oocytes - growth & development ; Ovarian Follicle - drug effects ; ovarian steroidogenesis ; Ovary - metabolism ; protein kinase A ; Steroids - biosynthesis ; teleost</subject><ispartof>Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology, 1997-05, Vol.117 (1), p.75-81</ispartof><rights>1997 Elsevier Science Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-b650a294d4ac2676775312eed25ffd9e9f23b42b4e0bdc065ab43414e75ae4fc3</citedby><cites>FETCH-LOGICAL-c391t-b650a294d4ac2676775312eed25ffd9e9f23b42b4e0bdc065ab43414e75ae4fc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9185330$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cerdà, J</creatorcontrib><creatorcontrib>Petrino, T.R</creatorcontrib><creatorcontrib>Landin, A.M</creatorcontrib><creatorcontrib>Lin, Y-W.P</creatorcontrib><title>Effects of the Isoquinolinesulfonamide H-8 on Fundulus heteroclitus Ovarian Follicles: Role of Cyclic Nucleotide-Dependent Protein Kinases on Steroidogenesis and Oocyte Maturation In Vitro</title><title>Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology</title><addtitle>Comp Biochem Physiol C Pharmacol Toxicol Endocrinol</addtitle><description>The possible role of cyclic nucleotide-dependent protein kinases in mediating the stimulatory actions of
Fundulus heteroclitus pituitary extract (FPE) during ovarian steroidogenesis and oocyte maturation
in vitro was investigated. Follicle-enclosed oocytes were cultured in the presence of FPE and/or N-[2-(Methylamino)ethyl]-5-isoquinolinesulfonamide (H-8), a compound that inhibits protein kinase A (PKA) and cGMP-dependent protein kinase. H-8 alone (0.1–1 mM) promoted oocyte germinal vesicle breakdown (GVBD) in a dose-dependent manner. However, the process of GVBD initiated by H-8 was much slower than that triggered by 17
α,20
β-dihydroxy-4-pregnen-3-one (17,20
βP), the natural inducer of oocyte maturation in
F. heteroclitus. Treatment with H-8 also increased 17,20
βP production by the follicles and the accumulation of this steroid in the media was much slower than that initiated by FPE. However, in contrast to the FPE action on the oocyte, which is mediated by 17,20
βP, the stimulatory action of H-8 on GVBD appears to be independent of follicular steroid production, since aminoglutethimide (AGI), an inhibitor of steroidogenesis, did not-block H-8-induced GVBD while inhibiting H-8-induced 17,20
βP production. Moreover, addition of H-8 to FPE-treated follicles significantly reduced 17,20
βP secretion and the percentage of GVBD. These results provide further support for the involvement of PKA in the mechanism by which FPE stimulates ovarian steroidogenesis in
F. heteroclitus. Furthermore, the fact that H-8 alone increased 17,20
βP levels may imply that basal follicular production of this steroid could be induced by inactivation of cyclic nucleotide-dependent protein kinases. Data also indicate that inhibition of PKA and/or c-GMP-dependent protein kinase in the oocyte may be involved in the mechanism leading to resumption of meiosis in this species.</description><subject>Animals</subject><subject>Brackish</subject><subject>cGMP-dependent protein kinase</subject><subject>Cyclic AMP-Dependent Protein Kinases - adverse effects</subject><subject>Cyclic GMP-Dependent Protein Kinases - physiology</subject><subject>Female</subject><subject>Fishes</subject><subject>Fundulus heteroclitus</subject><subject>gonadotropin</subject><subject>Isoquinolines - antagonists & inhibitors</subject><subject>Isoquinolines - pharmacology</subject><subject>isoquinolinesulfonamide H-8</subject><subject>Killifishes - metabolism</subject><subject>Marine</subject><subject>oocyte maturation</subject><subject>Oocytes - drug effects</subject><subject>Oocytes - enzymology</subject><subject>Oocytes - growth & development</subject><subject>Ovarian Follicle - drug effects</subject><subject>ovarian steroidogenesis</subject><subject>Ovary - metabolism</subject><subject>protein kinase A</subject><subject>Steroids - biosynthesis</subject><subject>teleost</subject><issn>0742-8413</issn><issn>1367-8280</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1uEzEUhWcBKqXwCJW8QrAY6r-ZidkgFFoatRBEga3lsa-pkWOntqdS3o2Hw9NE3XZl-Z5zz5Hu1zSnBL8nmPRnN3jgtF1wwt6K_h3GlPXt4llz_Dh-0bzM-S_GmFDSHzVHgiw6xvBx8-_cWtAlo2hRuQW0yvFuciF6FyBP3sagNs4AumwXKAZ0MQUz-SmjWyiQovau1M_6XiWnqhq9d9pD_oB-RA9z5nJXPRp9m-o4lprUfoYtBAOhoO8pFnABXbmgMuQ5_2ZOdSb-gVrvMlLBoHXUuwLoqypTUsVV1yqg366k-Kp5bpXP8PrwnjS_Ls5_Li_b6_WX1fLTdauZIKUd-w4rKrjhStN-6IehY4QCGNpZawQIS9nI6cgBj0bjvlMjZ5xwGDoF3Gp20rzZ525TvQ7kIjcua_BeBYhTloPAHcZCPGkkPe06xmdjtzfqFHNOYOU2uY1KO0mwnJHKB6RyZidFLx-QykXdOz0UTOMGzOPWgWfVP-51qOe4d5Bk1g6CBuNSxSxNdE80_Afzd7b2</recordid><startdate>19970501</startdate><enddate>19970501</enddate><creator>Cerdà, J</creator><creator>Petrino, T.R</creator><creator>Landin, A.M</creator><creator>Lin, Y-W.P</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TN</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>19970501</creationdate><title>Effects of the Isoquinolinesulfonamide H-8 on Fundulus heteroclitus Ovarian Follicles: Role of Cyclic Nucleotide-Dependent Protein Kinases on Steroidogenesis and Oocyte Maturation In Vitro</title><author>Cerdà, J ; Petrino, T.R ; Landin, A.M ; Lin, Y-W.P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-b650a294d4ac2676775312eed25ffd9e9f23b42b4e0bdc065ab43414e75ae4fc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Brackish</topic><topic>cGMP-dependent protein kinase</topic><topic>Cyclic AMP-Dependent Protein Kinases - adverse effects</topic><topic>Cyclic GMP-Dependent Protein Kinases - physiology</topic><topic>Female</topic><topic>Fishes</topic><topic>Fundulus heteroclitus</topic><topic>gonadotropin</topic><topic>Isoquinolines - antagonists & inhibitors</topic><topic>Isoquinolines - pharmacology</topic><topic>isoquinolinesulfonamide H-8</topic><topic>Killifishes - metabolism</topic><topic>Marine</topic><topic>oocyte maturation</topic><topic>Oocytes - drug effects</topic><topic>Oocytes - enzymology</topic><topic>Oocytes - growth & development</topic><topic>Ovarian Follicle - drug effects</topic><topic>ovarian steroidogenesis</topic><topic>Ovary - metabolism</topic><topic>protein kinase A</topic><topic>Steroids - biosynthesis</topic><topic>teleost</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cerdà, J</creatorcontrib><creatorcontrib>Petrino, T.R</creatorcontrib><creatorcontrib>Landin, A.M</creatorcontrib><creatorcontrib>Lin, Y-W.P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oceanic Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cerdà, J</au><au>Petrino, T.R</au><au>Landin, A.M</au><au>Lin, Y-W.P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of the Isoquinolinesulfonamide H-8 on Fundulus heteroclitus Ovarian Follicles: Role of Cyclic Nucleotide-Dependent Protein Kinases on Steroidogenesis and Oocyte Maturation In Vitro</atitle><jtitle>Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology</jtitle><addtitle>Comp Biochem Physiol C Pharmacol Toxicol Endocrinol</addtitle><date>1997-05-01</date><risdate>1997</risdate><volume>117</volume><issue>1</issue><spage>75</spage><epage>81</epage><pages>75-81</pages><issn>0742-8413</issn><issn>1367-8280</issn><abstract>The possible role of cyclic nucleotide-dependent protein kinases in mediating the stimulatory actions of
Fundulus heteroclitus pituitary extract (FPE) during ovarian steroidogenesis and oocyte maturation
in vitro was investigated. Follicle-enclosed oocytes were cultured in the presence of FPE and/or N-[2-(Methylamino)ethyl]-5-isoquinolinesulfonamide (H-8), a compound that inhibits protein kinase A (PKA) and cGMP-dependent protein kinase. H-8 alone (0.1–1 mM) promoted oocyte germinal vesicle breakdown (GVBD) in a dose-dependent manner. However, the process of GVBD initiated by H-8 was much slower than that triggered by 17
α,20
β-dihydroxy-4-pregnen-3-one (17,20
βP), the natural inducer of oocyte maturation in
F. heteroclitus. Treatment with H-8 also increased 17,20
βP production by the follicles and the accumulation of this steroid in the media was much slower than that initiated by FPE. However, in contrast to the FPE action on the oocyte, which is mediated by 17,20
βP, the stimulatory action of H-8 on GVBD appears to be independent of follicular steroid production, since aminoglutethimide (AGI), an inhibitor of steroidogenesis, did not-block H-8-induced GVBD while inhibiting H-8-induced 17,20
βP production. Moreover, addition of H-8 to FPE-treated follicles significantly reduced 17,20
βP secretion and the percentage of GVBD. These results provide further support for the involvement of PKA in the mechanism by which FPE stimulates ovarian steroidogenesis in
F. heteroclitus. Furthermore, the fact that H-8 alone increased 17,20
βP levels may imply that basal follicular production of this steroid could be induced by inactivation of cyclic nucleotide-dependent protein kinases. Data also indicate that inhibition of PKA and/or c-GMP-dependent protein kinase in the oocyte may be involved in the mechanism leading to resumption of meiosis in this species.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>9185330</pmid><doi>10.1016/S0742-8413(96)00236-8</doi><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 0742-8413 |
| ispartof | Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology, 1997-05, Vol.117 (1), p.75-81 |
| issn | 0742-8413 1367-8280 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16255349 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Brackish cGMP-dependent protein kinase Cyclic AMP-Dependent Protein Kinases - adverse effects Cyclic GMP-Dependent Protein Kinases - physiology Female Fishes Fundulus heteroclitus gonadotropin Isoquinolines - antagonists & inhibitors Isoquinolines - pharmacology isoquinolinesulfonamide H-8 Killifishes - metabolism Marine oocyte maturation Oocytes - drug effects Oocytes - enzymology Oocytes - growth & development Ovarian Follicle - drug effects ovarian steroidogenesis Ovary - metabolism protein kinase A Steroids - biosynthesis teleost |
| title | Effects of the Isoquinolinesulfonamide H-8 on Fundulus heteroclitus Ovarian Follicles: Role of Cyclic Nucleotide-Dependent Protein Kinases on Steroidogenesis and Oocyte Maturation In Vitro |
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