Immunomagnetic isolation of fetal rat gonocytes

An efficient method to obtain highly enriched populations of viable gonocytes from rat embryos at Day 18 and Day 20 postcoitum (pc) is described. Single-cell suspensions with high cell yield were obtained by a collagenase/trypsin digestion of the decapsulated testis. The gonocytes were purified by a...

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Veröffentlicht in:American journal of reproductive immunology and microbiology 1997-07, Vol.38 (1), p.39-45
Hauptverfasser: van den Ham, R, van Pelt, AMM, de Miguel, MP, van Kooten, PJS, Walther, N, van Dissel-Emiliani, FMF
Format: Artikel
Sprache:eng
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