LC-MS/MS determination of cinacalcet enantiomers in rat plasma on Chirobiotic V column in polar ionic mode: application to a pharmacokinetic study
ABSTRACT A simple and selective polar ionic liquid chromatography–tandem mass spectrometric method for separation and determination of cinacalcet enantiomers in rat plasma was developed and validated. The chromatographic separation was accomplished on a Chirobiotic V column packed with vancomycin as...
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| Veröffentlicht in: | Biomedical chromatography 2014-12, Vol.28 (12), p.1846-1853 |
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| container_title | Biomedical chromatography |
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| creator | Ramisetti, Nageswara Rao Bompelli, Sravan |
| description | ABSTRACT
A simple and selective polar ionic liquid chromatography–tandem mass spectrometric method for separation and determination of cinacalcet enantiomers in rat plasma was developed and validated. The chromatographic separation was accomplished on a Chirobiotic V column packed with vancomycin as a chiral stationary phase using 2.5 mm ammonium formate in 100% methanol as a mobile phase in an isocratic mode of elution at a flow rate of 1.0 mL/min. The analytes were extracted from rat plasma by precipitating the proteins with acetonitrile. The developed method exhibited a linear dynamic range over 0.5–500 ng/mL in rat plasma for both enantiomers. The method was successfully applied to study the pharmacokinetics after a single dose by oral administration of 10 mg/kg of cinacalcet enantiomers to healthy male Wistar rats. Copyright © 2014 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/bmc.3229 |
| format | Article |
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A simple and selective polar ionic liquid chromatography–tandem mass spectrometric method for separation and determination of cinacalcet enantiomers in rat plasma was developed and validated. The chromatographic separation was accomplished on a Chirobiotic V column packed with vancomycin as a chiral stationary phase using 2.5 mm ammonium formate in 100% methanol as a mobile phase in an isocratic mode of elution at a flow rate of 1.0 mL/min. The analytes were extracted from rat plasma by precipitating the proteins with acetonitrile. The developed method exhibited a linear dynamic range over 0.5–500 ng/mL in rat plasma for both enantiomers. The method was successfully applied to study the pharmacokinetics after a single dose by oral administration of 10 mg/kg of cinacalcet enantiomers to healthy male Wistar rats. Copyright © 2014 John Wiley & Sons, Ltd.</description><identifier>ISSN: 0269-3879</identifier><identifier>EISSN: 1099-0801</identifier><identifier>DOI: 10.1002/bmc.3229</identifier><identifier>PMID: 24816613</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Administration, Oral ; Animals ; Chromatography, Liquid - instrumentation ; Chromatography, Liquid - methods ; cinacalcet ; Cinacalcet Hydrochloride ; Drug Stability ; enantiomers ; Hydrogen-Ion Concentration ; Limit of Detection ; Male ; Naphthalenes - administration & dosage ; Naphthalenes - blood ; Naphthalenes - chemistry ; Naphthalenes - pharmacokinetics ; polar ionic ; rat plasma ; Rats ; Rats, Wistar ; Reproducibility of Results ; Sodium Chloride ; Stereoisomerism ; Tandem Mass Spectrometry - methods ; vancomycin ; Vancomycin - chemistry</subject><ispartof>Biomedical chromatography, 2014-12, Vol.28 (12), p.1846-1853</ispartof><rights>Copyright © 2014 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3599-898b12500029d9fe5fc25b1dbb9bf76dcc110b8fd0f04f653c0e6db1d9eac61e3</citedby><cites>FETCH-LOGICAL-c3599-898b12500029d9fe5fc25b1dbb9bf76dcc110b8fd0f04f653c0e6db1d9eac61e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbmc.3229$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbmc.3229$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24816613$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ramisetti, Nageswara Rao</creatorcontrib><creatorcontrib>Bompelli, Sravan</creatorcontrib><title>LC-MS/MS determination of cinacalcet enantiomers in rat plasma on Chirobiotic V column in polar ionic mode: application to a pharmacokinetic study</title><title>Biomedical chromatography</title><addtitle>Biomed. Chromatogr</addtitle><description>ABSTRACT
A simple and selective polar ionic liquid chromatography–tandem mass spectrometric method for separation and determination of cinacalcet enantiomers in rat plasma was developed and validated. The chromatographic separation was accomplished on a Chirobiotic V column packed with vancomycin as a chiral stationary phase using 2.5 mm ammonium formate in 100% methanol as a mobile phase in an isocratic mode of elution at a flow rate of 1.0 mL/min. The analytes were extracted from rat plasma by precipitating the proteins with acetonitrile. The developed method exhibited a linear dynamic range over 0.5–500 ng/mL in rat plasma for both enantiomers. The method was successfully applied to study the pharmacokinetics after a single dose by oral administration of 10 mg/kg of cinacalcet enantiomers to healthy male Wistar rats. Copyright © 2014 John Wiley & Sons, Ltd.</description><subject>Administration, Oral</subject><subject>Animals</subject><subject>Chromatography, Liquid - instrumentation</subject><subject>Chromatography, Liquid - methods</subject><subject>cinacalcet</subject><subject>Cinacalcet Hydrochloride</subject><subject>Drug Stability</subject><subject>enantiomers</subject><subject>Hydrogen-Ion Concentration</subject><subject>Limit of Detection</subject><subject>Male</subject><subject>Naphthalenes - administration & dosage</subject><subject>Naphthalenes - blood</subject><subject>Naphthalenes - chemistry</subject><subject>Naphthalenes - pharmacokinetics</subject><subject>polar ionic</subject><subject>rat plasma</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Reproducibility of Results</subject><subject>Sodium Chloride</subject><subject>Stereoisomerism</subject><subject>Tandem Mass Spectrometry - methods</subject><subject>vancomycin</subject><subject>Vancomycin - chemistry</subject><issn>0269-3879</issn><issn>1099-0801</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU2P0zAQhi0EYsuCxC9APnLJrj8aJ-YGESxIbZEoH0fLccZas3EcbEfQv8EvxlXLcuLk0eiZx5p5EXpOyRUlhF333lxxxuQDtKJEyoq0hD5EK8KErHjbyAv0JKXvhBApWPMYXbB1S4WgfIV-b7pqu7_e7vEAGaJ3k84uTDhYbEpt9GggY5j0VNoeYsJuwlFnPI86eY0L2t26GHoXsjP4KzZhXPx0pOYw6oiLrPR9GOAV1vM8OnP6IAes8Xyro9cm3LkJjuMpL8PhKXpk9Zjg2fm9RF_evf3cva82H28-dK83leF12bGVbU9ZXZZicpAWamtY3dOh72VvGzEYQynpWzsQS9ZW1NwQEEMBJGgjKPBL9PLknWP4sUDKyrtkYBz1BGFJigpW83XT0vofamJIKYJVc3Rex4OiRB0TUCUBdUygoC_O1qX3MNyDf09egOoE_HQjHP4rUm-23Vl45l3K8Oue1_FOiYY3tfq2u1Gf6ma_Y-1e7fgf-Aagtg</recordid><startdate>201412</startdate><enddate>201412</enddate><creator>Ramisetti, Nageswara Rao</creator><creator>Bompelli, Sravan</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201412</creationdate><title>LC-MS/MS determination of cinacalcet enantiomers in rat plasma on Chirobiotic V column in polar ionic mode: application to a pharmacokinetic study</title><author>Ramisetti, Nageswara Rao ; Bompelli, Sravan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3599-898b12500029d9fe5fc25b1dbb9bf76dcc110b8fd0f04f653c0e6db1d9eac61e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Administration, Oral</topic><topic>Animals</topic><topic>Chromatography, Liquid - instrumentation</topic><topic>Chromatography, Liquid - methods</topic><topic>cinacalcet</topic><topic>Cinacalcet Hydrochloride</topic><topic>Drug Stability</topic><topic>enantiomers</topic><topic>Hydrogen-Ion Concentration</topic><topic>Limit of Detection</topic><topic>Male</topic><topic>Naphthalenes - administration & dosage</topic><topic>Naphthalenes - blood</topic><topic>Naphthalenes - chemistry</topic><topic>Naphthalenes - pharmacokinetics</topic><topic>polar ionic</topic><topic>rat plasma</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Reproducibility of Results</topic><topic>Sodium Chloride</topic><topic>Stereoisomerism</topic><topic>Tandem Mass Spectrometry - methods</topic><topic>vancomycin</topic><topic>Vancomycin - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ramisetti, Nageswara Rao</creatorcontrib><creatorcontrib>Bompelli, Sravan</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biomedical chromatography</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ramisetti, Nageswara Rao</au><au>Bompelli, Sravan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>LC-MS/MS determination of cinacalcet enantiomers in rat plasma on Chirobiotic V column in polar ionic mode: application to a pharmacokinetic study</atitle><jtitle>Biomedical chromatography</jtitle><addtitle>Biomed. Chromatogr</addtitle><date>2014-12</date><risdate>2014</risdate><volume>28</volume><issue>12</issue><spage>1846</spage><epage>1853</epage><pages>1846-1853</pages><issn>0269-3879</issn><eissn>1099-0801</eissn><abstract>ABSTRACT
A simple and selective polar ionic liquid chromatography–tandem mass spectrometric method for separation and determination of cinacalcet enantiomers in rat plasma was developed and validated. The chromatographic separation was accomplished on a Chirobiotic V column packed with vancomycin as a chiral stationary phase using 2.5 mm ammonium formate in 100% methanol as a mobile phase in an isocratic mode of elution at a flow rate of 1.0 mL/min. The analytes were extracted from rat plasma by precipitating the proteins with acetonitrile. The developed method exhibited a linear dynamic range over 0.5–500 ng/mL in rat plasma for both enantiomers. The method was successfully applied to study the pharmacokinetics after a single dose by oral administration of 10 mg/kg of cinacalcet enantiomers to healthy male Wistar rats. Copyright © 2014 John Wiley & Sons, Ltd.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>24816613</pmid><doi>10.1002/bmc.3229</doi><tpages>8</tpages></addata></record> |
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| ispartof | Biomedical chromatography, 2014-12, Vol.28 (12), p.1846-1853 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Administration, Oral Animals Chromatography, Liquid - instrumentation Chromatography, Liquid - methods cinacalcet Cinacalcet Hydrochloride Drug Stability enantiomers Hydrogen-Ion Concentration Limit of Detection Male Naphthalenes - administration & dosage Naphthalenes - blood Naphthalenes - chemistry Naphthalenes - pharmacokinetics polar ionic rat plasma Rats Rats, Wistar Reproducibility of Results Sodium Chloride Stereoisomerism Tandem Mass Spectrometry - methods vancomycin Vancomycin - chemistry |
| title | LC-MS/MS determination of cinacalcet enantiomers in rat plasma on Chirobiotic V column in polar ionic mode: application to a pharmacokinetic study |
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