Biotransformation of warfarin by the fungus Beauveria bassiana
Biotransformation of racemic warfarin by the fungus Beauveria bassiana IMI 12939 was studied as part of an investigation into alternative methods for the production of drug metabolites. Resting cell suspensions in buffer were able to biotransform warfarin. High activity occurred within a broad pH ra...
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| Veröffentlicht in: | Applied microbiology and biotechnology 1992-05, Vol.37 (2), p.169-175 |
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| container_title | Applied microbiology and biotechnology |
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| creator | GRIFFITHS, D. A BROWN, D. E JEZEQUEL, S. G |
| description | Biotransformation of racemic warfarin by the fungus Beauveria bassiana IMI 12939 was studied as part of an investigation into alternative methods for the production of drug metabolites. Resting cell suspensions in buffer were able to biotransform warfarin. High activity occurred within a broad pH range (6.0-9.0) with an optimum at 7.0. The major metabolites were 4'-hydroxywarfarin (metabolite I), warfarin alpha-diketone (metabolite B) and diastereomers of novel tautomeric ring-cleaved warfarin (metabolites E and F). The exact quantitative metabolite profile obtained following warfarin metabolism was shown to be highly dependent upon the cell concentration. Kinetic evidence suggested the operation of multiple enzyme activities. |
| doi_str_mv | 10.1007/BF00178165 |
| format | Article |
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Kinetic evidence suggested the operation of multiple enzyme activities.</description><subject>4'-hydroxywarfarin</subject><subject>Beauveria bassiana</subject><subject>Bioconversions. Hemisynthesis</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>metabolism</subject><subject>metabolites</subject><subject>Methods. Procedures. Technologies</subject><subject>stereochemistry</subject><subject>stereoisomers</subject><subject>warfarin</subject><subject>warfarin alpha -diketone</subject><issn>0175-7598</issn><issn>1432-0614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><recordid>eNpFkMFKxDAURYMoWEc3fkEW4kKoJmmaJhvBGRwVBtzourw0iUY6zZi0yvy9kRl0cx9czruLg9A5JdeUkOZmviSENpKK-gAVlFesJILyQ1Tkti6bWsljdJLSR6aYFKJAt3MfxghDciGuYfRhwMHhb4gOoh-w3uLx3WI3DW9TwnML05eNHrCGlDwMcIqOHPTJnu3vDL0u718Wj-Xq-eFpcbcqu4rVY2klGMt0DuBaKEOVMJSC0gasYUoq0jAnudSdqhTRyjaVgY4bA5JbR2k1Q5e73U0Mn5NNY7v2qbN9D4MNU2qpYHXFWZXBqx3YxZBStK7dRL-GuG0paX8Vtf-KMnyxX4XUQe-yh86nv4-aM8GyxB_lKmYr</recordid><startdate>19920501</startdate><enddate>19920501</enddate><creator>GRIFFITHS, D. A</creator><creator>BROWN, D. E</creator><creator>JEZEQUEL, S. 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Psychology</topic><topic>metabolism</topic><topic>metabolites</topic><topic>Methods. Procedures. Technologies</topic><topic>stereochemistry</topic><topic>stereoisomers</topic><topic>warfarin</topic><topic>warfarin alpha -diketone</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>GRIFFITHS, D. A</creatorcontrib><creatorcontrib>BROWN, D. E</creatorcontrib><creatorcontrib>JEZEQUEL, S. 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| ispartof | Applied microbiology and biotechnology, 1992-05, Vol.37 (2), p.169-175 |
| issn | 0175-7598 1432-0614 |
| language | eng |
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| source | Springer Nature - Complete Springer Journals |
| subjects | 4'-hydroxywarfarin Beauveria bassiana Bioconversions. Hemisynthesis Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology metabolism metabolites Methods. Procedures. Technologies stereochemistry stereoisomers warfarin warfarin alpha -diketone |
| title | Biotransformation of warfarin by the fungus Beauveria bassiana |
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