Evaluation of positive controls for the in vitro unscheduled DNA synthesis assay using hepatocytes from induced (Aroclor 1254) and uninduced male cynomolgus monkey
We have evaluated the use of four different positive control compounds for assessing UDS in monkey hepatocytes and have found three of these, methylmethanesulfonate, benzo[a]pyrene, and dimethylbenz[a]anthracene, to produce strong positive responses in vitro. Dimethylnitrosamine induced only weak re...
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| Veröffentlicht in: | Environmental and molecular mutagenesis 1997, Vol.30 (3), p.354-358 |
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| container_title | Environmental and molecular mutagenesis |
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| creator | Hamilton, Carol M. Dabbs, Jack E. Cunningham, Glenn D. Vernetti, Lawrence A. Mirsalis, Jon C. Snyder, Ronald D. |
| description | We have evaluated the use of four different positive control compounds for assessing UDS in monkey hepatocytes and have found three of these, methylmethanesulfonate, benzo[a]pyrene, and dimethylbenz[a]anthracene, to produce strong positive responses in vitro. Dimethylnitrosamine induced only weak responses. We also report that the strength of the response induced by procarcinogens was not enhanced in hepatocytes taken from Aroclor 1254‐pretreated monkeys, even though substantial induction of cytochrome P450 enzymes was demonstrated in these cells. These studies raise the question of the utility of employing an in vivo induction system to enhance the monkey UDS assay. Environ. Mol. Mutagen. 30:354–358, 1997 © 1997 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/(SICI)1098-2280(1997)30:3<354::AID-EM15>3.0.CO;2-C |
| format | Article |
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Dimethylnitrosamine induced only weak responses. We also report that the strength of the response induced by procarcinogens was not enhanced in hepatocytes taken from Aroclor 1254‐pretreated monkeys, even though substantial induction of cytochrome P450 enzymes was demonstrated in these cells. These studies raise the question of the utility of employing an in vivo induction system to enhance the monkey UDS assay. Environ. Mol. Mutagen. 30:354–358, 1997 © 1997 Wiley‐Liss, Inc.</description><identifier>ISSN: 0893-6692</identifier><identifier>EISSN: 1098-2280</identifier><identifier>DOI: 10.1002/(SICI)1098-2280(1997)30:3<354::AID-EM15>3.0.CO;2-C</identifier><identifier>PMID: 9366915</identifier><identifier>CODEN: EMMUEG</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>12-dimethylbenz[a]anthracene ; 7,12‐dimethylbenz[a]anthracene ; 9,10-Dimethyl-1,2-benzanthracene - toxicity ; Animals ; Aroclor 1254 ; Aroclors - pharmacology ; Benzo(a)pyrene - toxicity ; benzo[a]pyrene ; Biological and medical sciences ; Biotransformation ; Chemical mutagenesis ; dimethylnitrosamine ; Dimethylnitrosamine - toxicity ; DNA Repair ; Enzyme Induction ; hepatocytes ; in vitro ; Liver ; Macaca fascicularis ; Male ; Medical sciences ; Methyl Methanesulfonate - toxicity ; methylmethanesulfonate ; monkeys ; Mutagenicity Tests - methods ; Toxicology ; UDS</subject><ispartof>Environmental and molecular mutagenesis, 1997, Vol.30 (3), p.354-358</ispartof><rights>Copyright © 1997 Wiley‐Liss, Inc.</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3865-849144bb57bd35e9c70b5f1d8c890e4e9a0f65a5ae0c0423df55f42973b2f9f63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F%28SICI%291098-2280%281997%2930%3A3%3C354%3A%3AAID-EM15%3E3.0.CO%3B2-C$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F%28SICI%291098-2280%281997%2930%3A3%3C354%3A%3AAID-EM15%3E3.0.CO%3B2-C$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,4010,27904,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2062833$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9366915$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hamilton, Carol M.</creatorcontrib><creatorcontrib>Dabbs, Jack E.</creatorcontrib><creatorcontrib>Cunningham, Glenn D.</creatorcontrib><creatorcontrib>Vernetti, Lawrence A.</creatorcontrib><creatorcontrib>Mirsalis, Jon C.</creatorcontrib><creatorcontrib>Snyder, Ronald D.</creatorcontrib><title>Evaluation of positive controls for the in vitro unscheduled DNA synthesis assay using hepatocytes from induced (Aroclor 1254) and uninduced male cynomolgus monkey</title><title>Environmental and molecular mutagenesis</title><addtitle>Environ. Mol. Mutagen</addtitle><description>We have evaluated the use of four different positive control compounds for assessing UDS in monkey hepatocytes and have found three of these, methylmethanesulfonate, benzo[a]pyrene, and dimethylbenz[a]anthracene, to produce strong positive responses in vitro. Dimethylnitrosamine induced only weak responses. We also report that the strength of the response induced by procarcinogens was not enhanced in hepatocytes taken from Aroclor 1254‐pretreated monkeys, even though substantial induction of cytochrome P450 enzymes was demonstrated in these cells. These studies raise the question of the utility of employing an in vivo induction system to enhance the monkey UDS assay. Environ. Mol. Mutagen. 30:354–358, 1997 © 1997 Wiley‐Liss, Inc.</description><subject>12-dimethylbenz[a]anthracene</subject><subject>7,12‐dimethylbenz[a]anthracene</subject><subject>9,10-Dimethyl-1,2-benzanthracene - toxicity</subject><subject>Animals</subject><subject>Aroclor 1254</subject><subject>Aroclors - pharmacology</subject><subject>Benzo(a)pyrene - toxicity</subject><subject>benzo[a]pyrene</subject><subject>Biological and medical sciences</subject><subject>Biotransformation</subject><subject>Chemical mutagenesis</subject><subject>dimethylnitrosamine</subject><subject>Dimethylnitrosamine - toxicity</subject><subject>DNA Repair</subject><subject>Enzyme Induction</subject><subject>hepatocytes</subject><subject>in vitro</subject><subject>Liver</subject><subject>Macaca fascicularis</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Methyl Methanesulfonate - toxicity</subject><subject>methylmethanesulfonate</subject><subject>monkeys</subject><subject>Mutagenicity Tests - methods</subject><subject>Toxicology</subject><subject>UDS</subject><issn>0893-6692</issn><issn>1098-2280</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kduO0zAQhiMEWpaFR0DyBULtRYoPcRIXhFSypa20bMVJcDdyHWdrNolLnBTyPLwoDi3lAsSVNZ6Zb37pC4IpwROCMX02er_KVmOCRRpSmuIRESIZMzxlLxiPptPZ6jKcvyH8JZvgSbZ-TsPsTnB-Gr8bnONUsDCOBb0fPHDuC8aERIKeBWeC-V_Cz4Mf870sO9kaWyNboJ11pjV7jZSt28aWDhW2Qe1WI1OjvfFfqKud2uq8K3WOLq9nyPW17zvjkHRO9qhzpr5BW72TrVV9qz2isZXfzzvlV0azxqrSQwnl0RjJOvfE381Klv50X9vKljedQ5Wtb3X_MLhXyNLpR8f3Ivj4ev4hW4ZX68Uqm12FiqUxD9NIkCjabHiyyRnXQiV4wwuSpyoVWEdaSFzEXHKpscIRZXnBeRFRkbANLUQRs4vg6YG7a-zXTrsWKuOULktZa9s5IDHljCTUD747DKrGOtfoAnaNqWTTA8EwmAMYzMGgAgYVMJgDhoGBNwfgzcFgztcYsjVQyDz08fF6t6l0fkIeVfn-k2NfOiXLopG1Mu40RnFMU8b-ZPtmSt3_Fey_uf4R61ftoeEBalyrv5-gsrmFOGEJh0_XC8CLz8tXy-QtEPYTJajQiQ</recordid><startdate>1997</startdate><enddate>1997</enddate><creator>Hamilton, Carol M.</creator><creator>Dabbs, Jack E.</creator><creator>Cunningham, Glenn D.</creator><creator>Vernetti, Lawrence A.</creator><creator>Mirsalis, Jon C.</creator><creator>Snyder, Ronald D.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>1997</creationdate><title>Evaluation of positive controls for the in vitro unscheduled DNA synthesis assay using hepatocytes from induced (Aroclor 1254) and uninduced male cynomolgus monkey</title><author>Hamilton, Carol M. ; Dabbs, Jack E. ; Cunningham, Glenn D. ; Vernetti, Lawrence A. ; Mirsalis, Jon C. ; Snyder, Ronald D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3865-849144bb57bd35e9c70b5f1d8c890e4e9a0f65a5ae0c0423df55f42973b2f9f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>12-dimethylbenz[a]anthracene</topic><topic>7,12‐dimethylbenz[a]anthracene</topic><topic>9,10-Dimethyl-1,2-benzanthracene - toxicity</topic><topic>Animals</topic><topic>Aroclor 1254</topic><topic>Aroclors - pharmacology</topic><topic>Benzo(a)pyrene - toxicity</topic><topic>benzo[a]pyrene</topic><topic>Biological and medical sciences</topic><topic>Biotransformation</topic><topic>Chemical mutagenesis</topic><topic>dimethylnitrosamine</topic><topic>Dimethylnitrosamine - toxicity</topic><topic>DNA Repair</topic><topic>Enzyme Induction</topic><topic>hepatocytes</topic><topic>in vitro</topic><topic>Liver</topic><topic>Macaca fascicularis</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Methyl Methanesulfonate - toxicity</topic><topic>methylmethanesulfonate</topic><topic>monkeys</topic><topic>Mutagenicity Tests - methods</topic><topic>Toxicology</topic><topic>UDS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hamilton, Carol M.</creatorcontrib><creatorcontrib>Dabbs, Jack E.</creatorcontrib><creatorcontrib>Cunningham, Glenn D.</creatorcontrib><creatorcontrib>Vernetti, Lawrence A.</creatorcontrib><creatorcontrib>Mirsalis, Jon C.</creatorcontrib><creatorcontrib>Snyder, Ronald D.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Environmental and molecular mutagenesis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hamilton, Carol M.</au><au>Dabbs, Jack E.</au><au>Cunningham, Glenn D.</au><au>Vernetti, Lawrence A.</au><au>Mirsalis, Jon C.</au><au>Snyder, Ronald D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of positive controls for the in vitro unscheduled DNA synthesis assay using hepatocytes from induced (Aroclor 1254) and uninduced male cynomolgus monkey</atitle><jtitle>Environmental and molecular mutagenesis</jtitle><addtitle>Environ. Mol. Mutagen</addtitle><date>1997</date><risdate>1997</risdate><volume>30</volume><issue>3</issue><spage>354</spage><epage>358</epage><pages>354-358</pages><issn>0893-6692</issn><eissn>1098-2280</eissn><coden>EMMUEG</coden><abstract>We have evaluated the use of four different positive control compounds for assessing UDS in monkey hepatocytes and have found three of these, methylmethanesulfonate, benzo[a]pyrene, and dimethylbenz[a]anthracene, to produce strong positive responses in vitro. Dimethylnitrosamine induced only weak responses. We also report that the strength of the response induced by procarcinogens was not enhanced in hepatocytes taken from Aroclor 1254‐pretreated monkeys, even though substantial induction of cytochrome P450 enzymes was demonstrated in these cells. These studies raise the question of the utility of employing an in vivo induction system to enhance the monkey UDS assay. Environ. Mol. Mutagen. 30:354–358, 1997 © 1997 Wiley‐Liss, Inc.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>9366915</pmid><doi>10.1002/(SICI)1098-2280(1997)30:3<354::AID-EM15>3.0.CO;2-C</doi><tpages>5</tpages></addata></record> |
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| identifier | ISSN: 0893-6692 |
| ispartof | Environmental and molecular mutagenesis, 1997, Vol.30 (3), p.354-358 |
| issn | 0893-6692 1098-2280 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | 12-dimethylbenz[a]anthracene 7,12‐dimethylbenz[a]anthracene 9,10-Dimethyl-1,2-benzanthracene - toxicity Animals Aroclor 1254 Aroclors - pharmacology Benzo(a)pyrene - toxicity benzo[a]pyrene Biological and medical sciences Biotransformation Chemical mutagenesis dimethylnitrosamine Dimethylnitrosamine - toxicity DNA Repair Enzyme Induction hepatocytes in vitro Liver Macaca fascicularis Male Medical sciences Methyl Methanesulfonate - toxicity methylmethanesulfonate monkeys Mutagenicity Tests - methods Toxicology UDS |
| title | Evaluation of positive controls for the in vitro unscheduled DNA synthesis assay using hepatocytes from induced (Aroclor 1254) and uninduced male cynomolgus monkey |
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