Transformation of the fungal forest pathogen Dothistroma pini to hygromycin resistance

The forest pathogen Dothistroma pini, which produces the polyketide toxin, dothistromin, was transformed with the pAN7-1 plasmid containing the Escherichia coli hygromycin B phosphotransferase gene (hph). The frequency of hygromycin resistant transformants was 9–48 μg−1 DNA. Southern blot analysis i...

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Veröffentlicht in:	Mycological research 1997-10, Vol.101 (10), p.1247-1250
Hauptverfasser:	BRADSHAW, R. E., BIDLAKE, A., FORESTER, N., SCOTT, D. B.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology Fungal plant pathogens Genetic engineering Genetic technics Methods. Procedures. Technologies Pathology, epidemiology, host-fungus relationships. Damages, economic importance Phytopathology. Animal pests. Plant and forest protection Transgenic animals and transgenic plants Transgenic plants
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