Redox enzyme engineering: conversion of human glutathione reductase into a trypanothione reductase

The substrate specificity of the human enzyme glutathione reductase was changed from its natural substrate glutathione to trypanothione [N1,N8-bis(glutathionyl)spermidine] by site-directed mutagenesis of two residues. The glutathione analogue, trypanothione, is the natural substrate for trypanothion...

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Veröffentlicht in:	Biochemistry (Easton) 1991-06, Vol.30 (25), p.6124-6127
Hauptverfasser:	Bradley, Mark, Buecheler, Uwe S, Walsh, Christopher T
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Binding Sites Cloning, Molecular conversion Escherichia coli - genetics Glutathione - analogs & derivatives Glutathione - metabolism glutathione reductase Glutathione Reductase - chemistry Glutathione Reductase - genetics Humans Kinetics man Molecular Sequence Data Mutagenesis, Site-Directed NADH, NADPH Oxidoreductases - chemistry NADH, NADPH Oxidoreductases - genetics Oxidation-Reduction Protein Engineering - methods site-directed mutagenesis Spermidine - analogs & derivatives Spermidine - metabolism Substrate Specificity Trypanosoma - enzymology trypanothione reductase X-Ray Diffraction
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