Evaluation of methods to extract ergosterol for quantitation of soil fungal biomass

Two methods were evaluated to extract ergosterol for quantitation of fungal biomass in Marshan, Zurich, and Batavia soils. Yields of ergosterol from hyphae and from fungal-colonized soil were greater when fungal tissue was extracted with an alkaline solvent mixture than when base was added to neutra...

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Veröffentlicht in:	Soil biology & biochemistry 1992, Vol.24 (3), p.189-198
Hauptverfasser:	Davis, Mark W., Lamar, Richard T.
Format:	Artikel
Sprache:	eng
Schlagworte:	acetone acetone-dry ice bath Agronomy. Soil science and plant productions Biochemistry and biology Biological and medical sciences biomass Chemical, physicochemical, biochemical and biological properties clay loam soils copper sulfate ergosterol evaluation extraction free ergosterol freeze drying freezing frozen storage Fundamental and applied biological sciences. Psychology Generalities Hapludalfs heplaquolls hyphae hyphal mats magnesium sulfate Mollisols Phanerochaete chrysosporium Physics, chemistry, biochemistry and biology of agricultural and forest soils Populus tremuloides pretreatment quantitative analysis sandy loam soils soil analysis soil fungi soil inoculation Soil science stability total alkali-extractable ergosterol total neutral-extractable ergosterol wood chips
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