Lead-induced alteration of heparan sulfate proteoglycans in cultured vascular endothelial cells
We investigated the alterations of heparan sulfate proteoglycans produced by vascular endothelial cells after exposure to lead. Bovine aortic endothelial cells were cultured and metabolically labeled with [ 3H]glucosamine and [ 35S]sulfate in the presence of lead chloride at 10 μM. Radiolabeled HSPG...
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| Veröffentlicht in: | Toxicology (Amsterdam) 1997-03, Vol.118 (1), p.1-10 |
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| creator | Kaji, Toshiyuki Ohkawara, Susumu Nakajima, Mie Yamamoto, Chika Fujiwara, Yasuyuki Miyajima, Sawako Koizumi, Fumitomo |
| description | We investigated the alterations of heparan sulfate proteoglycans produced by vascular endothelial cells after exposure to lead. Bovine aortic endothelial cells were cultured and metabolically labeled with [
3H]glucosamine and [
35S]sulfate in the presence of lead chloride at 10 μM. Radiolabeled HSPGs were separated by ion-exchange chromatography and either their hydrodynamic size or the length of heparan sulfate chains were characterized by gel filtration. It was found that lead markedly suppresses the incorporation of the radioactive precursors into HSPGs in the cell layer; the incorporation into chondroitin/dermatan sulfate proteoglycans was decreased by the metal only slightly. The suppression by lead occurred in the low molecular weight subclass of HSPGs rather than the high molecular weight subclass. However, the length of heparan sulfate chains was not changed by the metal. A sodium dodecyl sulfate-polyacrylamide gel electrophoresis of [
35S]methionine-labeled proteoglycans after heparitinase digestion showed that there were slightly more HSPG core proteins without a change of the size in lead-treated cell layer. It was, therefore, suggested that vascular endothelial cell layer after exposure to lead has more HSPG core proteins with fewer heparan sulfate chains without a change in length. |
| doi_str_mv | 10.1016/S0300-483X(96)03582-2 |
| format | Article |
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3H]glucosamine and [
35S]sulfate in the presence of lead chloride at 10 μM. Radiolabeled HSPGs were separated by ion-exchange chromatography and either their hydrodynamic size or the length of heparan sulfate chains were characterized by gel filtration. It was found that lead markedly suppresses the incorporation of the radioactive precursors into HSPGs in the cell layer; the incorporation into chondroitin/dermatan sulfate proteoglycans was decreased by the metal only slightly. The suppression by lead occurred in the low molecular weight subclass of HSPGs rather than the high molecular weight subclass. However, the length of heparan sulfate chains was not changed by the metal. A sodium dodecyl sulfate-polyacrylamide gel electrophoresis of [
35S]methionine-labeled proteoglycans after heparitinase digestion showed that there were slightly more HSPG core proteins without a change of the size in lead-treated cell layer. It was, therefore, suggested that vascular endothelial cell layer after exposure to lead has more HSPG core proteins with fewer heparan sulfate chains without a change in length.</description><identifier>ISSN: 0300-483X</identifier><identifier>EISSN: 1879-3185</identifier><identifier>DOI: 10.1016/S0300-483X(96)03582-2</identifier><identifier>PMID: 9074649</identifier><identifier>CODEN: TXICDD</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Animals ; Aorta ; Biological and medical sciences ; Cattle ; Cells, Cultured ; Chemical and industrial products toxicology. Toxic occupational diseases ; Chromatography, DEAE-Cellulose ; Chromatography, Gel ; Culture Media, Conditioned ; Electrophoresis, Polyacrylamide Gel ; Endothelial cells ; Endothelium, Vascular - chemistry ; Endothelium, Vascular - cytology ; Endothelium, Vascular - drug effects ; Glucosamine - analysis ; Glucosamine - metabolism ; Glycoproteins - analysis ; Glycosaminoglycans - metabolism ; Heparan sulfate ; Heparan Sulfate Proteoglycans ; Heparitin Sulfate - chemistry ; Heparitin Sulfate - metabolism ; Hyaluronic Acid - analysis ; Lead ; Lead - toxicity ; Medical sciences ; Metals and various inorganic compounds ; Molecular Weight ; Papain - metabolism ; Polysaccharide-Lyases - metabolism ; Proteoglycan ; Proteoglycans - chemistry ; Proteoglycans - metabolism ; Sulfates - analysis ; Sulfates - metabolism ; Toxicology ; Vascular</subject><ispartof>Toxicology (Amsterdam), 1997-03, Vol.118 (1), p.1-10</ispartof><rights>1997</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c517t-bac20ac1649d00eac960e1b16e2a1e0507bfd58f2de9319db6908557ef1f37863</citedby><cites>FETCH-LOGICAL-c517t-bac20ac1649d00eac960e1b16e2a1e0507bfd58f2de9319db6908557ef1f37863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0300483X96035822$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2615771$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9074649$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kaji, Toshiyuki</creatorcontrib><creatorcontrib>Ohkawara, Susumu</creatorcontrib><creatorcontrib>Nakajima, Mie</creatorcontrib><creatorcontrib>Yamamoto, Chika</creatorcontrib><creatorcontrib>Fujiwara, Yasuyuki</creatorcontrib><creatorcontrib>Miyajima, Sawako</creatorcontrib><creatorcontrib>Koizumi, Fumitomo</creatorcontrib><title>Lead-induced alteration of heparan sulfate proteoglycans in cultured vascular endothelial cells</title><title>Toxicology (Amsterdam)</title><addtitle>Toxicology</addtitle><description>We investigated the alterations of heparan sulfate proteoglycans produced by vascular endothelial cells after exposure to lead. Bovine aortic endothelial cells were cultured and metabolically labeled with [
3H]glucosamine and [
35S]sulfate in the presence of lead chloride at 10 μM. Radiolabeled HSPGs were separated by ion-exchange chromatography and either their hydrodynamic size or the length of heparan sulfate chains were characterized by gel filtration. It was found that lead markedly suppresses the incorporation of the radioactive precursors into HSPGs in the cell layer; the incorporation into chondroitin/dermatan sulfate proteoglycans was decreased by the metal only slightly. The suppression by lead occurred in the low molecular weight subclass of HSPGs rather than the high molecular weight subclass. However, the length of heparan sulfate chains was not changed by the metal. A sodium dodecyl sulfate-polyacrylamide gel electrophoresis of [
35S]methionine-labeled proteoglycans after heparitinase digestion showed that there were slightly more HSPG core proteins without a change of the size in lead-treated cell layer. It was, therefore, suggested that vascular endothelial cell layer after exposure to lead has more HSPG core proteins with fewer heparan sulfate chains without a change in length.</description><subject>Animals</subject><subject>Aorta</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Cells, Cultured</subject><subject>Chemical and industrial products toxicology. Toxic occupational diseases</subject><subject>Chromatography, DEAE-Cellulose</subject><subject>Chromatography, Gel</subject><subject>Culture Media, Conditioned</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Endothelial cells</subject><subject>Endothelium, Vascular - chemistry</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - drug effects</subject><subject>Glucosamine - analysis</subject><subject>Glucosamine - metabolism</subject><subject>Glycoproteins - analysis</subject><subject>Glycosaminoglycans - metabolism</subject><subject>Heparan sulfate</subject><subject>Heparan Sulfate Proteoglycans</subject><subject>Heparitin Sulfate - chemistry</subject><subject>Heparitin Sulfate - metabolism</subject><subject>Hyaluronic Acid - analysis</subject><subject>Lead</subject><subject>Lead - toxicity</subject><subject>Medical sciences</subject><subject>Metals and various inorganic compounds</subject><subject>Molecular Weight</subject><subject>Papain - metabolism</subject><subject>Polysaccharide-Lyases - metabolism</subject><subject>Proteoglycan</subject><subject>Proteoglycans - chemistry</subject><subject>Proteoglycans - metabolism</subject><subject>Sulfates - analysis</subject><subject>Sulfates - metabolism</subject><subject>Toxicology</subject><subject>Vascular</subject><issn>0300-483X</issn><issn>1879-3185</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2KFDEURoMoYzv6CANZiOii9CappCorGQb_oMGFCu5CKrlxIumqNkkNzNubnm56O6sE7rnJx_kIuWLwngFTH36AAOj6Ufx-q9U7EHLkHX9CNmwcdCfYKJ-SzRl5Tl6U8hcAuOjVBbnQMPSq1xtitmh9F2e_OvTUporZ1rjMdAn0Fvc225mWNQVbke7zUnH5k-6dnQuNM3Vrqmtue3e2tLvNFGe_1FtM0SbqMKXykjwLNhV8dTovya_Pn37efO223798u7nedk6yoXaTdRysYy2TB0DrtAJkE1PILUOQMEzByzFwj1ow7SelYZRywMCCGEYlLsmb47st5L8VSzW7WA4J7IzLWgxTXAgp-ONg38QwBg2UR9DlpZSMwexz3Nl8bxiYQwPmoQFz0Gu0Mg8NmMMHV6cP1mmH_rx1Ut7mr0_zJs2m0Ay7WM4YV0wOA2vYxyOGzdpdxGyKizi3lmJGV41f4iNB_gPhiKPu</recordid><startdate>19970314</startdate><enddate>19970314</enddate><creator>Kaji, Toshiyuki</creator><creator>Ohkawara, Susumu</creator><creator>Nakajima, Mie</creator><creator>Yamamoto, Chika</creator><creator>Fujiwara, Yasuyuki</creator><creator>Miyajima, Sawako</creator><creator>Koizumi, Fumitomo</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>C1K</scope><scope>SOI</scope><scope>7U7</scope></search><sort><creationdate>19970314</creationdate><title>Lead-induced alteration of heparan sulfate proteoglycans in cultured vascular endothelial cells</title><author>Kaji, Toshiyuki ; Ohkawara, Susumu ; Nakajima, Mie ; Yamamoto, Chika ; Fujiwara, Yasuyuki ; Miyajima, Sawako ; Koizumi, Fumitomo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c517t-bac20ac1649d00eac960e1b16e2a1e0507bfd58f2de9319db6908557ef1f37863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Aorta</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Cells, Cultured</topic><topic>Chemical and industrial products toxicology. Toxic occupational diseases</topic><topic>Chromatography, DEAE-Cellulose</topic><topic>Chromatography, Gel</topic><topic>Culture Media, Conditioned</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Endothelial cells</topic><topic>Endothelium, Vascular - chemistry</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - drug effects</topic><topic>Glucosamine - analysis</topic><topic>Glucosamine - metabolism</topic><topic>Glycoproteins - analysis</topic><topic>Glycosaminoglycans - metabolism</topic><topic>Heparan sulfate</topic><topic>Heparan Sulfate Proteoglycans</topic><topic>Heparitin Sulfate - chemistry</topic><topic>Heparitin Sulfate - metabolism</topic><topic>Hyaluronic Acid - analysis</topic><topic>Lead</topic><topic>Lead - toxicity</topic><topic>Medical sciences</topic><topic>Metals and various inorganic compounds</topic><topic>Molecular Weight</topic><topic>Papain - metabolism</topic><topic>Polysaccharide-Lyases - metabolism</topic><topic>Proteoglycan</topic><topic>Proteoglycans - chemistry</topic><topic>Proteoglycans - metabolism</topic><topic>Sulfates - analysis</topic><topic>Sulfates - metabolism</topic><topic>Toxicology</topic><topic>Vascular</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kaji, Toshiyuki</creatorcontrib><creatorcontrib>Ohkawara, Susumu</creatorcontrib><creatorcontrib>Nakajima, Mie</creatorcontrib><creatorcontrib>Yamamoto, Chika</creatorcontrib><creatorcontrib>Fujiwara, Yasuyuki</creatorcontrib><creatorcontrib>Miyajima, Sawako</creatorcontrib><creatorcontrib>Koizumi, Fumitomo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><collection>Toxicology Abstracts</collection><jtitle>Toxicology (Amsterdam)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kaji, Toshiyuki</au><au>Ohkawara, Susumu</au><au>Nakajima, Mie</au><au>Yamamoto, Chika</au><au>Fujiwara, Yasuyuki</au><au>Miyajima, Sawako</au><au>Koizumi, Fumitomo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lead-induced alteration of heparan sulfate proteoglycans in cultured vascular endothelial cells</atitle><jtitle>Toxicology (Amsterdam)</jtitle><addtitle>Toxicology</addtitle><date>1997-03-14</date><risdate>1997</risdate><volume>118</volume><issue>1</issue><spage>1</spage><epage>10</epage><pages>1-10</pages><issn>0300-483X</issn><eissn>1879-3185</eissn><coden>TXICDD</coden><abstract>We investigated the alterations of heparan sulfate proteoglycans produced by vascular endothelial cells after exposure to lead. Bovine aortic endothelial cells were cultured and metabolically labeled with [
3H]glucosamine and [
35S]sulfate in the presence of lead chloride at 10 μM. Radiolabeled HSPGs were separated by ion-exchange chromatography and either their hydrodynamic size or the length of heparan sulfate chains were characterized by gel filtration. It was found that lead markedly suppresses the incorporation of the radioactive precursors into HSPGs in the cell layer; the incorporation into chondroitin/dermatan sulfate proteoglycans was decreased by the metal only slightly. The suppression by lead occurred in the low molecular weight subclass of HSPGs rather than the high molecular weight subclass. However, the length of heparan sulfate chains was not changed by the metal. A sodium dodecyl sulfate-polyacrylamide gel electrophoresis of [
35S]methionine-labeled proteoglycans after heparitinase digestion showed that there were slightly more HSPG core proteins without a change of the size in lead-treated cell layer. It was, therefore, suggested that vascular endothelial cell layer after exposure to lead has more HSPG core proteins with fewer heparan sulfate chains without a change in length.</abstract><cop>Shannon</cop><cop>Amsterdam</cop><pub>Elsevier Ireland Ltd</pub><pmid>9074649</pmid><doi>10.1016/S0300-483X(96)03582-2</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0300-483X |
| ispartof | Toxicology (Amsterdam), 1997-03, Vol.118 (1), p.1-10 |
| issn | 0300-483X 1879-3185 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16233532 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Aorta Biological and medical sciences Cattle Cells, Cultured Chemical and industrial products toxicology. Toxic occupational diseases Chromatography, DEAE-Cellulose Chromatography, Gel Culture Media, Conditioned Electrophoresis, Polyacrylamide Gel Endothelial cells Endothelium, Vascular - chemistry Endothelium, Vascular - cytology Endothelium, Vascular - drug effects Glucosamine - analysis Glucosamine - metabolism Glycoproteins - analysis Glycosaminoglycans - metabolism Heparan sulfate Heparan Sulfate Proteoglycans Heparitin Sulfate - chemistry Heparitin Sulfate - metabolism Hyaluronic Acid - analysis Lead Lead - toxicity Medical sciences Metals and various inorganic compounds Molecular Weight Papain - metabolism Polysaccharide-Lyases - metabolism Proteoglycan Proteoglycans - chemistry Proteoglycans - metabolism Sulfates - analysis Sulfates - metabolism Toxicology Vascular |
| title | Lead-induced alteration of heparan sulfate proteoglycans in cultured vascular endothelial cells |
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