Moraxella catarrhalis is susceptible to antimicrobial photodynamic therapy with Photofrin
Background and Objective Moraxella catarrhalis is a significant cause of pediatric otitis media (OM), which is the most prevalent bacterial infection in children and primary reason for antibiotic administration in this population. Moreover, biofilm formation has been implicated as a primary mechanis...
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| Veröffentlicht in: | Lasers in surgery and medicine 2014-11, Vol.46 (9), p.712-717 |
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| creator | Luke-Marshall, Nicole R. Mang, Thomas S. Hansen, Lisa A. Campagnari, Anthony A. |
| description | Background and Objective
Moraxella catarrhalis is a significant cause of pediatric otitis media (OM), which is the most prevalent bacterial infection in children and primary reason for antibiotic administration in this population. Moreover, biofilm formation has been implicated as a primary mechanism of chronic or recurrent OM disease. As bacterial biofilms are inherently resistant to most antibiotics and these complex structures also present a significant challenge to the immune system, there is a clear need to identify novel antimicrobial approaches to treat OM infections. In this study, we evaluated the potential efficacy of antibacterial photodynamic therapy (aPDT) with porfimer sodium (Photofrin (PF)) against planktonic as well as biofilm‐associated M. catarrhalis.
Materials and Methods
The bactericidal activity of aPDT with PF was assessed against multiple recent clinical isolates of M. catarrhalis grown planktonically as well as in biofilms. The bactericidal activity of PF‐aPDT was quantified by enumeration of colony forming units post‐treatment. The effect of aPDT on M. catarrhalis biofilms was further investigated with scanning electron microscopy (SEM) imaging.
Results
aPDT with PF significantly reduced M. catarrhalis viability. Although PF‐aPDT caused higher killing in planktonic grown organisms (5–6 log kill), biofilm grown bacteria also demonstrated a statistically significant reduction in viable organisms (3–4 log decrease in recoverable bacteria) following treatment as compared to saline only controls (P |
| doi_str_mv | 10.1002/lsm.22287 |
| format | Article |
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Moraxella catarrhalis is a significant cause of pediatric otitis media (OM), which is the most prevalent bacterial infection in children and primary reason for antibiotic administration in this population. Moreover, biofilm formation has been implicated as a primary mechanism of chronic or recurrent OM disease. As bacterial biofilms are inherently resistant to most antibiotics and these complex structures also present a significant challenge to the immune system, there is a clear need to identify novel antimicrobial approaches to treat OM infections. In this study, we evaluated the potential efficacy of antibacterial photodynamic therapy (aPDT) with porfimer sodium (Photofrin (PF)) against planktonic as well as biofilm‐associated M. catarrhalis.
Materials and Methods
The bactericidal activity of aPDT with PF was assessed against multiple recent clinical isolates of M. catarrhalis grown planktonically as well as in biofilms. The bactericidal activity of PF‐aPDT was quantified by enumeration of colony forming units post‐treatment. The effect of aPDT on M. catarrhalis biofilms was further investigated with scanning electron microscopy (SEM) imaging.
Results
aPDT with PF significantly reduced M. catarrhalis viability. Although PF‐aPDT caused higher killing in planktonic grown organisms (5–6 log kill), biofilm grown bacteria also demonstrated a statistically significant reduction in viable organisms (3–4 log decrease in recoverable bacteria) following treatment as compared to saline only controls (P < 0.01). SEM studies indicated the PF‐aPDT treated bacteria exhibited prominent morphological changes with visibly distorted cell membranes.
Conclusions
aPDT with PF elicits significant bactericidal activity against both planktonic and biofilm‐associated M. catarrhalis, suggesting this technology warrants further analysis as a potential novel antimicrobial treatment for acute or recurrent OM. Lasers Surg. Med. 46:712–717, 2014. © 2014 Wiley Periodicals, Inc.</description><identifier>ISSN: 0196-8092</identifier><identifier>EISSN: 1096-9101</identifier><identifier>DOI: 10.1002/lsm.22287</identifier><identifier>PMID: 25154610</identifier><identifier>CODEN: LSMEDI</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>antimicrobial photodynamic therapy ; biofilm ; Biofilms - drug effects ; Biofilms - growth & development ; Biofilms - radiation effects ; Dihematoporphyrin Ether - pharmacology ; gram-negative bacteria ; Lasers, Dye ; Lasers, Solid-State ; Microbial Viability - drug effects ; Microbial Viability - radiation effects ; Moraxella (Branhamella) catarrhalis - drug effects ; Moraxella (Branhamella) catarrhalis - growth & development ; Moraxella (Branhamella) catarrhalis - radiation effects ; Moraxella catarrhalis ; otitis media ; Photochemotherapy ; photofrin ; Photosensitizing Agents - pharmacology</subject><ispartof>Lasers in surgery and medicine, 2014-11, Vol.46 (9), p.712-717</ispartof><rights>2014 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5317-1ee4cef1ae304f22ac25a65f1daefac0782e7a1164fa3e4c56d659f4438729f13</citedby><cites>FETCH-LOGICAL-c5317-1ee4cef1ae304f22ac25a65f1daefac0782e7a1164fa3e4c56d659f4438729f13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Flsm.22287$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Flsm.22287$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25154610$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Luke-Marshall, Nicole R.</creatorcontrib><creatorcontrib>Mang, Thomas S.</creatorcontrib><creatorcontrib>Hansen, Lisa A.</creatorcontrib><creatorcontrib>Campagnari, Anthony A.</creatorcontrib><title>Moraxella catarrhalis is susceptible to antimicrobial photodynamic therapy with Photofrin</title><title>Lasers in surgery and medicine</title><addtitle>Lasers Surg. Med</addtitle><description>Background and Objective
Moraxella catarrhalis is a significant cause of pediatric otitis media (OM), which is the most prevalent bacterial infection in children and primary reason for antibiotic administration in this population. Moreover, biofilm formation has been implicated as a primary mechanism of chronic or recurrent OM disease. As bacterial biofilms are inherently resistant to most antibiotics and these complex structures also present a significant challenge to the immune system, there is a clear need to identify novel antimicrobial approaches to treat OM infections. In this study, we evaluated the potential efficacy of antibacterial photodynamic therapy (aPDT) with porfimer sodium (Photofrin (PF)) against planktonic as well as biofilm‐associated M. catarrhalis.
Materials and Methods
The bactericidal activity of aPDT with PF was assessed against multiple recent clinical isolates of M. catarrhalis grown planktonically as well as in biofilms. The bactericidal activity of PF‐aPDT was quantified by enumeration of colony forming units post‐treatment. The effect of aPDT on M. catarrhalis biofilms was further investigated with scanning electron microscopy (SEM) imaging.
Results
aPDT with PF significantly reduced M. catarrhalis viability. Although PF‐aPDT caused higher killing in planktonic grown organisms (5–6 log kill), biofilm grown bacteria also demonstrated a statistically significant reduction in viable organisms (3–4 log decrease in recoverable bacteria) following treatment as compared to saline only controls (P < 0.01). SEM studies indicated the PF‐aPDT treated bacteria exhibited prominent morphological changes with visibly distorted cell membranes.
Conclusions
aPDT with PF elicits significant bactericidal activity against both planktonic and biofilm‐associated M. catarrhalis, suggesting this technology warrants further analysis as a potential novel antimicrobial treatment for acute or recurrent OM. Lasers Surg. Med. 46:712–717, 2014. © 2014 Wiley Periodicals, Inc.</description><subject>antimicrobial photodynamic therapy</subject><subject>biofilm</subject><subject>Biofilms - drug effects</subject><subject>Biofilms - growth & development</subject><subject>Biofilms - radiation effects</subject><subject>Dihematoporphyrin Ether - pharmacology</subject><subject>gram-negative bacteria</subject><subject>Lasers, Dye</subject><subject>Lasers, Solid-State</subject><subject>Microbial Viability - drug effects</subject><subject>Microbial Viability - radiation effects</subject><subject>Moraxella (Branhamella) catarrhalis - drug effects</subject><subject>Moraxella (Branhamella) catarrhalis - growth & development</subject><subject>Moraxella (Branhamella) catarrhalis - radiation effects</subject><subject>Moraxella catarrhalis</subject><subject>otitis media</subject><subject>Photochemotherapy</subject><subject>photofrin</subject><subject>Photosensitizing Agents - pharmacology</subject><issn>0196-8092</issn><issn>1096-9101</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkV1rFDEUhoModq1e-AdkwBu9mDYnXzNzqUVbYbcKKqI34ezsCZs6XyYZ2v33Zt22F4IgBBJOnvNyzvsy9hz4CXAuTrvYnwgh6uoBWwBvTNkAh4dswSG_a96II_YkxivOuRS8esyOhAatDPAF-74aA95Q12HRYsIQttj5WOQT59jSlPy6oyKNBQ7J974N49pjV0zbMY2b3YC5VKQtBZx2xbVP2-LT_scFPzxljxx2kZ7d3sfs6_t3X84uyuXH8w9nb5ZlqyVUJRCplhwgSa6cENgKjUY72CA5bHlVC6oQwCiHMqPabIxunFKyrkTjQB6zVwfdKYy_ZorJ9j5PnjcaaJyjBSOEAVMr8x8oKNOIGnRGX_6FXo1zGPIie0oqyH7LTL0-UNmXGAM5OwXfY9hZ4HYfjc3R2D_RZPbFreK87mlzT95lkYHTA3DtO9r9W8kuP6_uJMtDh4-Jbu47MPy0ppKVtt8uz-0PcQGXunlrV_I3fM-ndQ</recordid><startdate>201411</startdate><enddate>201411</enddate><creator>Luke-Marshall, Nicole R.</creator><creator>Mang, Thomas S.</creator><creator>Hansen, Lisa A.</creator><creator>Campagnari, Anthony A.</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>C1K</scope></search><sort><creationdate>201411</creationdate><title>Moraxella catarrhalis is susceptible to antimicrobial photodynamic therapy with Photofrin</title><author>Luke-Marshall, Nicole R. ; Mang, Thomas S. ; Hansen, Lisa A. ; Campagnari, Anthony A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5317-1ee4cef1ae304f22ac25a65f1daefac0782e7a1164fa3e4c56d659f4438729f13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>antimicrobial photodynamic therapy</topic><topic>biofilm</topic><topic>Biofilms - drug effects</topic><topic>Biofilms - growth & development</topic><topic>Biofilms - radiation effects</topic><topic>Dihematoporphyrin Ether - pharmacology</topic><topic>gram-negative bacteria</topic><topic>Lasers, Dye</topic><topic>Lasers, Solid-State</topic><topic>Microbial Viability - drug effects</topic><topic>Microbial Viability - radiation effects</topic><topic>Moraxella (Branhamella) catarrhalis - drug effects</topic><topic>Moraxella (Branhamella) catarrhalis - growth & development</topic><topic>Moraxella (Branhamella) catarrhalis - radiation effects</topic><topic>Moraxella catarrhalis</topic><topic>otitis media</topic><topic>Photochemotherapy</topic><topic>photofrin</topic><topic>Photosensitizing Agents - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Luke-Marshall, Nicole R.</creatorcontrib><creatorcontrib>Mang, Thomas S.</creatorcontrib><creatorcontrib>Hansen, Lisa A.</creatorcontrib><creatorcontrib>Campagnari, Anthony A.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Lasers in surgery and medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Luke-Marshall, Nicole R.</au><au>Mang, Thomas S.</au><au>Hansen, Lisa A.</au><au>Campagnari, Anthony A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Moraxella catarrhalis is susceptible to antimicrobial photodynamic therapy with Photofrin</atitle><jtitle>Lasers in surgery and medicine</jtitle><addtitle>Lasers Surg. Med</addtitle><date>2014-11</date><risdate>2014</risdate><volume>46</volume><issue>9</issue><spage>712</spage><epage>717</epage><pages>712-717</pages><issn>0196-8092</issn><eissn>1096-9101</eissn><coden>LSMEDI</coden><abstract>Background and Objective
Moraxella catarrhalis is a significant cause of pediatric otitis media (OM), which is the most prevalent bacterial infection in children and primary reason for antibiotic administration in this population. Moreover, biofilm formation has been implicated as a primary mechanism of chronic or recurrent OM disease. As bacterial biofilms are inherently resistant to most antibiotics and these complex structures also present a significant challenge to the immune system, there is a clear need to identify novel antimicrobial approaches to treat OM infections. In this study, we evaluated the potential efficacy of antibacterial photodynamic therapy (aPDT) with porfimer sodium (Photofrin (PF)) against planktonic as well as biofilm‐associated M. catarrhalis.
Materials and Methods
The bactericidal activity of aPDT with PF was assessed against multiple recent clinical isolates of M. catarrhalis grown planktonically as well as in biofilms. The bactericidal activity of PF‐aPDT was quantified by enumeration of colony forming units post‐treatment. The effect of aPDT on M. catarrhalis biofilms was further investigated with scanning electron microscopy (SEM) imaging.
Results
aPDT with PF significantly reduced M. catarrhalis viability. Although PF‐aPDT caused higher killing in planktonic grown organisms (5–6 log kill), biofilm grown bacteria also demonstrated a statistically significant reduction in viable organisms (3–4 log decrease in recoverable bacteria) following treatment as compared to saline only controls (P < 0.01). SEM studies indicated the PF‐aPDT treated bacteria exhibited prominent morphological changes with visibly distorted cell membranes.
Conclusions
aPDT with PF elicits significant bactericidal activity against both planktonic and biofilm‐associated M. catarrhalis, suggesting this technology warrants further analysis as a potential novel antimicrobial treatment for acute or recurrent OM. Lasers Surg. Med. 46:712–717, 2014. © 2014 Wiley Periodicals, Inc.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>25154610</pmid><doi>10.1002/lsm.22287</doi><tpages>6</tpages></addata></record> |
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| ispartof | Lasers in surgery and medicine, 2014-11, Vol.46 (9), p.712-717 |
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| subjects | antimicrobial photodynamic therapy biofilm Biofilms - drug effects Biofilms - growth & development Biofilms - radiation effects Dihematoporphyrin Ether - pharmacology gram-negative bacteria Lasers, Dye Lasers, Solid-State Microbial Viability - drug effects Microbial Viability - radiation effects Moraxella (Branhamella) catarrhalis - drug effects Moraxella (Branhamella) catarrhalis - growth & development Moraxella (Branhamella) catarrhalis - radiation effects Moraxella catarrhalis otitis media Photochemotherapy photofrin Photosensitizing Agents - pharmacology |
| title | Moraxella catarrhalis is susceptible to antimicrobial photodynamic therapy with Photofrin |
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