Functional profiling of EcaICE1 transcription factor gene from Eucalyptus camaldulensis involved in cold response in tobacco plants
A full-length cDNA designated as the EcaICE1 gene was isolated from Eucalyptus camaldulensis. The EcaICE1 gene was predicted to encode a MYC-like protein of 523 amino acids presenting a considerable degree of homology to currently identified plant inducer of CBF expression 1 (ICE1) proteins. RT-PCR...
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| Veröffentlicht in: | Journal of plant biochemistry and biotechnology 2014-04, Vol.23 (2), p.141-150 |
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| creator | Lin, Yuanzhen Zheng, Huiquan Zhang, Qian Liu, Chunxin Zhang, Zhiyi |
| description | A full-length cDNA designated as the EcaICE1 gene was isolated from Eucalyptus camaldulensis. The EcaICE1 gene was predicted to encode a MYC-like protein of 523 amino acids presenting a considerable degree of homology to currently identified plant inducer of CBF expression 1 (ICE1) proteins. RT-PCR assays indicated that EcaICE1 gene was expressed in a constitutive pattern in intact E. camaldulensis plants or the cold-treated (4 °C) plantlets. Strikingly, over-expression of the EcaICE1 gene in transgenic tobacco plants could enhance the tolerance to cold stress. While over-expression of the EcaICE1 gene resulted in a profound change of transcript level of C-repeat Binding Factor 1 (CBF1), CBF3 and group 2 LEA protein NtERD10C genes in transgenic tobacco plants. Further analysis revealed that EcaICE1 could function as a transcription activator in planta. Briefly, our results strongly suggest that the EcaICE1 gene is involved in the plant defense responses to low temperature. |
| doi_str_mv | 10.1007/s13562-013-0192-z |
| format | Article |
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The EcaICE1 gene was predicted to encode a MYC-like protein of 523 amino acids presenting a considerable degree of homology to currently identified plant inducer of CBF expression 1 (ICE1) proteins. RT-PCR assays indicated that EcaICE1 gene was expressed in a constitutive pattern in intact E. camaldulensis plants or the cold-treated (4 °C) plantlets. Strikingly, over-expression of the EcaICE1 gene in transgenic tobacco plants could enhance the tolerance to cold stress. While over-expression of the EcaICE1 gene resulted in a profound change of transcript level of C-repeat Binding Factor 1 (CBF1), CBF3 and group 2 LEA protein NtERD10C genes in transgenic tobacco plants. Further analysis revealed that EcaICE1 could function as a transcription activator in planta. Briefly, our results strongly suggest that the EcaICE1 gene is involved in the plant defense responses to low temperature.</description><identifier>ISSN: 0971-7811</identifier><identifier>EISSN: 0974-1275</identifier><identifier>DOI: 10.1007/s13562-013-0192-z</identifier><language>eng</language><publisher>India: Springer-Verlag</publisher><subject>amino acids ; Biomedical and Life Sciences ; Cell Biology ; cold ; cold stress ; cold tolerance ; complementary DNA ; Eucalyptus camaldulensis ; gene overexpression ; genes ; Life Sciences ; Original Article ; Plant Biochemistry ; plantlets ; Protein Science ; proteins ; Receptors ; reverse transcriptase polymerase chain reaction ; temperature ; tobacco ; transcription factors</subject><ispartof>Journal of plant biochemistry and biotechnology, 2014-04, Vol.23 (2), p.141-150</ispartof><rights>Society for Plant Biochemistry and Biotechnology 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c345t-16a472a57cf1651118df4e6953483f0c0644c336db37f2d354715d8c349c4f5a3</citedby><cites>FETCH-LOGICAL-c345t-16a472a57cf1651118df4e6953483f0c0644c336db37f2d354715d8c349c4f5a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s13562-013-0192-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s13562-013-0192-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Lin, Yuanzhen</creatorcontrib><creatorcontrib>Zheng, Huiquan</creatorcontrib><creatorcontrib>Zhang, Qian</creatorcontrib><creatorcontrib>Liu, Chunxin</creatorcontrib><creatorcontrib>Zhang, Zhiyi</creatorcontrib><title>Functional profiling of EcaICE1 transcription factor gene from Eucalyptus camaldulensis involved in cold response in tobacco plants</title><title>Journal of plant biochemistry and biotechnology</title><addtitle>J. Plant Biochem. Biotechnol</addtitle><description>A full-length cDNA designated as the EcaICE1 gene was isolated from Eucalyptus camaldulensis. The EcaICE1 gene was predicted to encode a MYC-like protein of 523 amino acids presenting a considerable degree of homology to currently identified plant inducer of CBF expression 1 (ICE1) proteins. RT-PCR assays indicated that EcaICE1 gene was expressed in a constitutive pattern in intact E. camaldulensis plants or the cold-treated (4 °C) plantlets. Strikingly, over-expression of the EcaICE1 gene in transgenic tobacco plants could enhance the tolerance to cold stress. While over-expression of the EcaICE1 gene resulted in a profound change of transcript level of C-repeat Binding Factor 1 (CBF1), CBF3 and group 2 LEA protein NtERD10C genes in transgenic tobacco plants. Further analysis revealed that EcaICE1 could function as a transcription activator in planta. Briefly, our results strongly suggest that the EcaICE1 gene is involved in the plant defense responses to low temperature.</description><subject>amino acids</subject><subject>Biomedical and Life Sciences</subject><subject>Cell Biology</subject><subject>cold</subject><subject>cold stress</subject><subject>cold tolerance</subject><subject>complementary DNA</subject><subject>Eucalyptus camaldulensis</subject><subject>gene overexpression</subject><subject>genes</subject><subject>Life Sciences</subject><subject>Original Article</subject><subject>Plant Biochemistry</subject><subject>plantlets</subject><subject>Protein Science</subject><subject>proteins</subject><subject>Receptors</subject><subject>reverse transcriptase polymerase chain reaction</subject><subject>temperature</subject><subject>tobacco</subject><subject>transcription factors</subject><issn>0971-7811</issn><issn>0974-1275</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNp9kE1rHSEUQIfQQtK0P6CruuxmUq-fM8vyeGkDgSzSrMU4-jD4dOqdCSTb_vH6Oll3IV7kHNHTdZ-BXgGl-hsCl4r1FHhbI-tfz7oLOmrRA9Py3b8Zej0AnHcfEJ8oFVJTcdH9uV6zW2LJNpG5lhBTzAdSAtk7e7PbA1mqzehqnE8QCdYtpZKDz56EWo5kvzqbXuZlReLs0aZpTT5jRBLzc0nPfmoDcSVNpHqcS0Z_OljKo3WukDnZvODH7n2wCf2nt_2ye7je_9r97G_vftzsvt_2jgu59KCs0MxK7QIoCQDDFIRXo-Ri4IE6qoRwnKvpkevAJi6FBjkNTR6dCNLyy-7rdm_76O_V42KOEZ1P7RG-rGhAMaYAxoE1FDbU1YJYfTBzjUdbXwxQcwputuCmBTen4Oa1OWxzsLH54Kt5KmttYfG_0pdNCrYYe6gRzcM9oyBo40clNP8LKMGOvQ</recordid><startdate>20140401</startdate><enddate>20140401</enddate><creator>Lin, Yuanzhen</creator><creator>Zheng, Huiquan</creator><creator>Zhang, Qian</creator><creator>Liu, Chunxin</creator><creator>Zhang, Zhiyi</creator><general>Springer-Verlag</general><general>Springer India</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20140401</creationdate><title>Functional profiling of EcaICE1 transcription factor gene from Eucalyptus camaldulensis involved in cold response in tobacco plants</title><author>Lin, Yuanzhen ; Zheng, Huiquan ; Zhang, Qian ; Liu, Chunxin ; Zhang, Zhiyi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c345t-16a472a57cf1651118df4e6953483f0c0644c336db37f2d354715d8c349c4f5a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>amino acids</topic><topic>Biomedical and Life Sciences</topic><topic>Cell Biology</topic><topic>cold</topic><topic>cold stress</topic><topic>cold tolerance</topic><topic>complementary DNA</topic><topic>Eucalyptus camaldulensis</topic><topic>gene overexpression</topic><topic>genes</topic><topic>Life Sciences</topic><topic>Original Article</topic><topic>Plant Biochemistry</topic><topic>plantlets</topic><topic>Protein Science</topic><topic>proteins</topic><topic>Receptors</topic><topic>reverse transcriptase polymerase chain reaction</topic><topic>temperature</topic><topic>tobacco</topic><topic>transcription factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lin, Yuanzhen</creatorcontrib><creatorcontrib>Zheng, Huiquan</creatorcontrib><creatorcontrib>Zhang, Qian</creatorcontrib><creatorcontrib>Liu, Chunxin</creatorcontrib><creatorcontrib>Zhang, Zhiyi</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of plant biochemistry and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lin, Yuanzhen</au><au>Zheng, Huiquan</au><au>Zhang, Qian</au><au>Liu, Chunxin</au><au>Zhang, Zhiyi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional profiling of EcaICE1 transcription factor gene from Eucalyptus camaldulensis involved in cold response in tobacco plants</atitle><jtitle>Journal of plant biochemistry and biotechnology</jtitle><stitle>J. Plant Biochem. Biotechnol</stitle><date>2014-04-01</date><risdate>2014</risdate><volume>23</volume><issue>2</issue><spage>141</spage><epage>150</epage><pages>141-150</pages><issn>0971-7811</issn><eissn>0974-1275</eissn><abstract>A full-length cDNA designated as the EcaICE1 gene was isolated from Eucalyptus camaldulensis. The EcaICE1 gene was predicted to encode a MYC-like protein of 523 amino acids presenting a considerable degree of homology to currently identified plant inducer of CBF expression 1 (ICE1) proteins. RT-PCR assays indicated that EcaICE1 gene was expressed in a constitutive pattern in intact E. camaldulensis plants or the cold-treated (4 °C) plantlets. Strikingly, over-expression of the EcaICE1 gene in transgenic tobacco plants could enhance the tolerance to cold stress. While over-expression of the EcaICE1 gene resulted in a profound change of transcript level of C-repeat Binding Factor 1 (CBF1), CBF3 and group 2 LEA protein NtERD10C genes in transgenic tobacco plants. Further analysis revealed that EcaICE1 could function as a transcription activator in planta. Briefly, our results strongly suggest that the EcaICE1 gene is involved in the plant defense responses to low temperature.</abstract><cop>India</cop><pub>Springer-Verlag</pub><doi>10.1007/s13562-013-0192-z</doi><tpages>10</tpages></addata></record> |
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| subjects | amino acids Biomedical and Life Sciences Cell Biology cold cold stress cold tolerance complementary DNA Eucalyptus camaldulensis gene overexpression genes Life Sciences Original Article Plant Biochemistry plantlets Protein Science proteins Receptors reverse transcriptase polymerase chain reaction temperature tobacco transcription factors |
| title | Functional profiling of EcaICE1 transcription factor gene from Eucalyptus camaldulensis involved in cold response in tobacco plants |
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