Regeneration of spermatogenesis in a mouse model of azoospermia by follicle-stimulating hormone and oestradiol
Summary Busulfan is a chemotherapeutic drug that induces sterility, azoospermia and testicular atrophy. To induce degeneration of spermatogenesis, we used different amounts of busulfan. Adult male C57Bl/6 mice were treated with 15, 30 and 45 mg kg−1 of busulfan. After 5 weeks, animals had daily inje...
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| Veröffentlicht in: | Andrologia 2014-12, Vol.46 (10), p.1098-1106 |
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| creator | Jafarian, A. Sadeghi, M. R. Pejhan, N. Salehkhou, S. Lakpour, N. Akhondi, M. M. |
| description | Summary
Busulfan is a chemotherapeutic drug that induces sterility, azoospermia and testicular atrophy. To induce degeneration of spermatogenesis, we used different amounts of busulfan. Adult male C57Bl/6 mice were treated with 15, 30 and 45 mg kg−1 of busulfan. After 5 weeks, animals had daily injections of 7.5 IU human follicle‐stimulating hormone (hFSH) and 12.5 μg kg−1 oestradiol benzoate (EB), separately or simultaneously. After this time, the animals were killed and blood samples were taken through cardiac puncture. Testes were used for histopathology experiments, DNA flow cytometry and RNA extraction for expression of c‐kit and cyclin B1 genes. EB unlike FSH has induced stimulatory effects on spermatogenesis, increased the level of serum testosterone 2‐fold and caused a 2‐fold increase in the number of haploid cells. The result showed that hFSH with EB multiplied EB stimulatory effects on spermatogenesis up to four times. Expression of c‐kit and cyclin B1 genes increased in EB and hFSH+EB groups. These findings suggest that EB regulates spermatogonial stem cells via hFSH. hFSH with EB had synergistic effect on regeneration of spermatogenesis. |
| doi_str_mv | 10.1111/and.12198 |
| format | Article |
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Busulfan is a chemotherapeutic drug that induces sterility, azoospermia and testicular atrophy. To induce degeneration of spermatogenesis, we used different amounts of busulfan. Adult male C57Bl/6 mice were treated with 15, 30 and 45 mg kg−1 of busulfan. After 5 weeks, animals had daily injections of 7.5 IU human follicle‐stimulating hormone (hFSH) and 12.5 μg kg−1 oestradiol benzoate (EB), separately or simultaneously. After this time, the animals were killed and blood samples were taken through cardiac puncture. Testes were used for histopathology experiments, DNA flow cytometry and RNA extraction for expression of c‐kit and cyclin B1 genes. EB unlike FSH has induced stimulatory effects on spermatogenesis, increased the level of serum testosterone 2‐fold and caused a 2‐fold increase in the number of haploid cells. The result showed that hFSH with EB multiplied EB stimulatory effects on spermatogenesis up to four times. Expression of c‐kit and cyclin B1 genes increased in EB and hFSH+EB groups. These findings suggest that EB regulates spermatogonial stem cells via hFSH. hFSH with EB had synergistic effect on regeneration of spermatogenesis.</description><identifier>ISSN: 0303-4569</identifier><identifier>EISSN: 1439-0272</identifier><identifier>DOI: 10.1111/and.12198</identifier><identifier>PMID: 24325627</identifier><language>eng</language><publisher>Germany: Blackwell Publishing Ltd</publisher><subject>Animals ; Azoospermia - chemically induced ; Azoospermia - drug therapy ; Azoospermia - metabolism ; Azoospermia - pathology ; Busulfan ; Cyclin B1 - genetics ; Cyclin B1 - metabolism ; Disease Models, Animal ; Estradiol - pharmacology ; Estradiol - therapeutic use ; Follicle Stimulating Hormone - pharmacology ; Follicle Stimulating Hormone - therapeutic use ; hFSH ; Male ; Mice ; oestradiol ; Proto-Oncogene Proteins c-kit - genetics ; Proto-Oncogene Proteins c-kit - metabolism ; spermatogenesis ; Spermatogenesis - drug effects ; Testis - drug effects ; Testis - metabolism ; Testis - pathology ; testosterone ; Testosterone - blood</subject><ispartof>Andrologia, 2014-12, Vol.46 (10), p.1098-1106</ispartof><rights>2013 Blackwell Verlag GmbH</rights><rights>2013 Blackwell Verlag GmbH.</rights><rights>Copyright © 2014 Blackwell Verlag GmbH</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4268-322bfd83e20d8a144ace3e1e113405004907c19b3c1d3d7074db79929a9874933</citedby><cites>FETCH-LOGICAL-c4268-322bfd83e20d8a144ace3e1e113405004907c19b3c1d3d7074db79929a9874933</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fand.12198$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fand.12198$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24325627$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jafarian, A.</creatorcontrib><creatorcontrib>Sadeghi, M. R.</creatorcontrib><creatorcontrib>Pejhan, N.</creatorcontrib><creatorcontrib>Salehkhou, S.</creatorcontrib><creatorcontrib>Lakpour, N.</creatorcontrib><creatorcontrib>Akhondi, M. M.</creatorcontrib><title>Regeneration of spermatogenesis in a mouse model of azoospermia by follicle-stimulating hormone and oestradiol</title><title>Andrologia</title><addtitle>Andrologia</addtitle><description>Summary
Busulfan is a chemotherapeutic drug that induces sterility, azoospermia and testicular atrophy. To induce degeneration of spermatogenesis, we used different amounts of busulfan. Adult male C57Bl/6 mice were treated with 15, 30 and 45 mg kg−1 of busulfan. After 5 weeks, animals had daily injections of 7.5 IU human follicle‐stimulating hormone (hFSH) and 12.5 μg kg−1 oestradiol benzoate (EB), separately or simultaneously. After this time, the animals were killed and blood samples were taken through cardiac puncture. Testes were used for histopathology experiments, DNA flow cytometry and RNA extraction for expression of c‐kit and cyclin B1 genes. EB unlike FSH has induced stimulatory effects on spermatogenesis, increased the level of serum testosterone 2‐fold and caused a 2‐fold increase in the number of haploid cells. The result showed that hFSH with EB multiplied EB stimulatory effects on spermatogenesis up to four times. Expression of c‐kit and cyclin B1 genes increased in EB and hFSH+EB groups. These findings suggest that EB regulates spermatogonial stem cells via hFSH. hFSH with EB had synergistic effect on regeneration of spermatogenesis.</description><subject>Animals</subject><subject>Azoospermia - chemically induced</subject><subject>Azoospermia - drug therapy</subject><subject>Azoospermia - metabolism</subject><subject>Azoospermia - pathology</subject><subject>Busulfan</subject><subject>Cyclin B1 - genetics</subject><subject>Cyclin B1 - metabolism</subject><subject>Disease Models, Animal</subject><subject>Estradiol - pharmacology</subject><subject>Estradiol - therapeutic use</subject><subject>Follicle Stimulating Hormone - pharmacology</subject><subject>Follicle Stimulating Hormone - therapeutic use</subject><subject>hFSH</subject><subject>Male</subject><subject>Mice</subject><subject>oestradiol</subject><subject>Proto-Oncogene Proteins c-kit - genetics</subject><subject>Proto-Oncogene Proteins c-kit - metabolism</subject><subject>spermatogenesis</subject><subject>Spermatogenesis - drug effects</subject><subject>Testis - drug effects</subject><subject>Testis - metabolism</subject><subject>Testis - pathology</subject><subject>testosterone</subject><subject>Testosterone - blood</subject><issn>0303-4569</issn><issn>1439-0272</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU1P3DAQhq2qqKyAQ_9AZamX9hDwV-L4SLdAKyGqIqoeLSeeUFPHXuxEsP319e4Ch0qdw4xkPfN63hmE3lJyTEucmGCPKaOqfYUWVHBVESbZa7QgnPBK1I3aR0c535ESopZSiDdonwnO6obJBQrXcAsBkplcDDgOOK8gjWaKm9fsMnYBGzzGOUPJFvyGMX9i3HLO4G6Nh-i96z1UeXLj7ItUuMW_YhpjAFzGwxHylIx10R-ivcH4DEdP9QD9OD-7WX6pLr9dfF2eXla9YE1bcca6wbYcGLGtoUKYHjhQoJQLUhcjisieqo731HIriRS2k0oxZVQrheL8AH3Y6a5SvJ_L93p0uQfvTYDiRdOGsVoJJZuCvv8HvYtzCmW6LUVK4qJQH3dUn2LOCQa9Sm40aa0p0Zs76GJUb-9Q2HdPinM3gn0hn7degJMd8OA8rP-vpE-vPj9LVrsOlyd4fOkw6bduJJe1_nl1oa-bT99vyHKpOf8LAiugIw</recordid><startdate>201412</startdate><enddate>201412</enddate><creator>Jafarian, A.</creator><creator>Sadeghi, M. R.</creator><creator>Pejhan, N.</creator><creator>Salehkhou, S.</creator><creator>Lakpour, N.</creator><creator>Akhondi, M. M.</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>201412</creationdate><title>Regeneration of spermatogenesis in a mouse model of azoospermia by follicle-stimulating hormone and oestradiol</title><author>Jafarian, A. ; Sadeghi, M. R. ; Pejhan, N. ; Salehkhou, S. ; Lakpour, N. ; Akhondi, M. M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4268-322bfd83e20d8a144ace3e1e113405004907c19b3c1d3d7074db79929a9874933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Azoospermia - chemically induced</topic><topic>Azoospermia - drug therapy</topic><topic>Azoospermia - metabolism</topic><topic>Azoospermia - pathology</topic><topic>Busulfan</topic><topic>Cyclin B1 - genetics</topic><topic>Cyclin B1 - metabolism</topic><topic>Disease Models, Animal</topic><topic>Estradiol - pharmacology</topic><topic>Estradiol - therapeutic use</topic><topic>Follicle Stimulating Hormone - pharmacology</topic><topic>Follicle Stimulating Hormone - therapeutic use</topic><topic>hFSH</topic><topic>Male</topic><topic>Mice</topic><topic>oestradiol</topic><topic>Proto-Oncogene Proteins c-kit - genetics</topic><topic>Proto-Oncogene Proteins c-kit - metabolism</topic><topic>spermatogenesis</topic><topic>Spermatogenesis - drug effects</topic><topic>Testis - drug effects</topic><topic>Testis - metabolism</topic><topic>Testis - pathology</topic><topic>testosterone</topic><topic>Testosterone - blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jafarian, A.</creatorcontrib><creatorcontrib>Sadeghi, M. R.</creatorcontrib><creatorcontrib>Pejhan, N.</creatorcontrib><creatorcontrib>Salehkhou, S.</creatorcontrib><creatorcontrib>Lakpour, N.</creatorcontrib><creatorcontrib>Akhondi, M. M.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Andrologia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jafarian, A.</au><au>Sadeghi, M. R.</au><au>Pejhan, N.</au><au>Salehkhou, S.</au><au>Lakpour, N.</au><au>Akhondi, M. M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regeneration of spermatogenesis in a mouse model of azoospermia by follicle-stimulating hormone and oestradiol</atitle><jtitle>Andrologia</jtitle><addtitle>Andrologia</addtitle><date>2014-12</date><risdate>2014</risdate><volume>46</volume><issue>10</issue><spage>1098</spage><epage>1106</epage><pages>1098-1106</pages><issn>0303-4569</issn><eissn>1439-0272</eissn><abstract>Summary
Busulfan is a chemotherapeutic drug that induces sterility, azoospermia and testicular atrophy. To induce degeneration of spermatogenesis, we used different amounts of busulfan. Adult male C57Bl/6 mice were treated with 15, 30 and 45 mg kg−1 of busulfan. After 5 weeks, animals had daily injections of 7.5 IU human follicle‐stimulating hormone (hFSH) and 12.5 μg kg−1 oestradiol benzoate (EB), separately or simultaneously. After this time, the animals were killed and blood samples were taken through cardiac puncture. Testes were used for histopathology experiments, DNA flow cytometry and RNA extraction for expression of c‐kit and cyclin B1 genes. EB unlike FSH has induced stimulatory effects on spermatogenesis, increased the level of serum testosterone 2‐fold and caused a 2‐fold increase in the number of haploid cells. The result showed that hFSH with EB multiplied EB stimulatory effects on spermatogenesis up to four times. Expression of c‐kit and cyclin B1 genes increased in EB and hFSH+EB groups. These findings suggest that EB regulates spermatogonial stem cells via hFSH. hFSH with EB had synergistic effect on regeneration of spermatogenesis.</abstract><cop>Germany</cop><pub>Blackwell Publishing Ltd</pub><pmid>24325627</pmid><doi>10.1111/and.12198</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Azoospermia - chemically induced Azoospermia - drug therapy Azoospermia - metabolism Azoospermia - pathology Busulfan Cyclin B1 - genetics Cyclin B1 - metabolism Disease Models, Animal Estradiol - pharmacology Estradiol - therapeutic use Follicle Stimulating Hormone - pharmacology Follicle Stimulating Hormone - therapeutic use hFSH Male Mice oestradiol Proto-Oncogene Proteins c-kit - genetics Proto-Oncogene Proteins c-kit - metabolism spermatogenesis Spermatogenesis - drug effects Testis - drug effects Testis - metabolism Testis - pathology testosterone Testosterone - blood |
| title | Regeneration of spermatogenesis in a mouse model of azoospermia by follicle-stimulating hormone and oestradiol |
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