Characterization of a specific erythromegakaryocytic enhancer within the glycoprotein IIb promoter
The gene coding for glycoprotein IIb (GPIIb), the alpha subunit of platelet integrin GPIIb/IIIa is an early and specific marker of the megakaryocytic lineage. Thus, studies on the regulation of this gene may provide helpful information on the mechanisms controlling cell specificity and differentiati...
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| Veröffentlicht in: | The Journal of biological chemistry 1992-05, Vol.267 (15), p.10370-10374 |
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| creator | PRANDINI, M.-H UZAN, G MARTIN, F THEVENON, D MARGUERIE, G |
| description | The gene coding for glycoprotein IIb (GPIIb), the alpha subunit of platelet integrin GPIIb/IIIa is an early and specific marker
of the megakaryocytic lineage. Thus, studies on the regulation of this gene may provide helpful information on the mechanisms
controlling cell specificity and differentiation in this lineage. The promoter region of this gene was isolated and analyzed
to understand its tissue-specific transcriptional activity. A region extending from nucleotides -414 to -554 was found to
be extremely important for the promoter function. Deletion of this region results in a 70% decrease of the promoter activity,
as measured in CAT assays. This region has the properties of an enhancer. It is able to activate a heterologous promoter,
in a distance- and orientation-independent manner, in both megakaryocytic and erythroid cells. This enhancer contains binding
sites for nuclear factors and mutation of these sites, individually or together, abolish the enhancer activity. These nuclear
factors are present in megakaryocytic and erythroid cell lineages, but they are absent in the other tested cells. One of the
sites, named domain D, contains a TTATC motif that may interact with the transcription factor GATA1, active in erythroid and
megakaryocytic cells. These results indicate that the promoter of a megakaryocytic gene contains a tissue specific enhancer,
active in both the erythroid and the megakaryocytic lineages, and may implicate the erythroid factor GATA1. |
| doi_str_mv | 10.1016/s0021-9258(19)50028-x |
| format | Article |
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of the megakaryocytic lineage. Thus, studies on the regulation of this gene may provide helpful information on the mechanisms
controlling cell specificity and differentiation in this lineage. The promoter region of this gene was isolated and analyzed
to understand its tissue-specific transcriptional activity. A region extending from nucleotides -414 to -554 was found to
be extremely important for the promoter function. Deletion of this region results in a 70% decrease of the promoter activity,
as measured in CAT assays. This region has the properties of an enhancer. It is able to activate a heterologous promoter,
in a distance- and orientation-independent manner, in both megakaryocytic and erythroid cells. This enhancer contains binding
sites for nuclear factors and mutation of these sites, individually or together, abolish the enhancer activity. These nuclear
factors are present in megakaryocytic and erythroid cell lineages, but they are absent in the other tested cells. One of the
sites, named domain D, contains a TTATC motif that may interact with the transcription factor GATA1, active in erythroid and
megakaryocytic cells. These results indicate that the promoter of a megakaryocytic gene contains a tissue specific enhancer,
active in both the erythroid and the megakaryocytic lineages, and may implicate the erythroid factor GATA1.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/s0021-9258(19)50028-x</identifier><identifier>PMID: 1587823</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: American Society for Biochemistry and Molecular Biology</publisher><subject>Base Sequence ; Biological and medical sciences ; Cell Line ; Chloramphenicol O-Acetyltransferase - metabolism ; Chromosome Deletion ; Electrophoresis, Polyacrylamide Gel ; Enhancer Elements, Genetic ; Erythroid Precursor Cells - metabolism ; Fundamental and applied biological sciences. Psychology ; HeLa Cells ; Humans ; Luciferases - metabolism ; Megakaryocytes - metabolism ; Methylation ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Mutagenesis, Site-Directed ; Plasmids ; Platelet Membrane Glycoproteins - genetics ; Promoter Regions, Genetic ; Transcription, Genetic ; Transcription. Transcription factor. Splicing. Rna processing ; Transfection</subject><ispartof>The Journal of biological chemistry, 1992-05, Vol.267 (15), p.10370-10374</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-d0d6685f045bd1467c76e66b4d7e70b45f27b83307382368a8a2440fd8c22d173</citedby><cites>FETCH-LOGICAL-c438t-d0d6685f045bd1467c76e66b4d7e70b45f27b83307382368a8a2440fd8c22d173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5353707$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1587823$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>PRANDINI, M.-H</creatorcontrib><creatorcontrib>UZAN, G</creatorcontrib><creatorcontrib>MARTIN, F</creatorcontrib><creatorcontrib>THEVENON, D</creatorcontrib><creatorcontrib>MARGUERIE, G</creatorcontrib><title>Characterization of a specific erythromegakaryocytic enhancer within the glycoprotein IIb promoter</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>The gene coding for glycoprotein IIb (GPIIb), the alpha subunit of platelet integrin GPIIb/IIIa is an early and specific marker
of the megakaryocytic lineage. Thus, studies on the regulation of this gene may provide helpful information on the mechanisms
controlling cell specificity and differentiation in this lineage. The promoter region of this gene was isolated and analyzed
to understand its tissue-specific transcriptional activity. A region extending from nucleotides -414 to -554 was found to
be extremely important for the promoter function. Deletion of this region results in a 70% decrease of the promoter activity,
as measured in CAT assays. This region has the properties of an enhancer. It is able to activate a heterologous promoter,
in a distance- and orientation-independent manner, in both megakaryocytic and erythroid cells. This enhancer contains binding
sites for nuclear factors and mutation of these sites, individually or together, abolish the enhancer activity. These nuclear
factors are present in megakaryocytic and erythroid cell lineages, but they are absent in the other tested cells. One of the
sites, named domain D, contains a TTATC motif that may interact with the transcription factor GATA1, active in erythroid and
megakaryocytic cells. These results indicate that the promoter of a megakaryocytic gene contains a tissue specific enhancer,
active in both the erythroid and the megakaryocytic lineages, and may implicate the erythroid factor GATA1.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Chloramphenicol O-Acetyltransferase - metabolism</subject><subject>Chromosome Deletion</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Enhancer Elements, Genetic</subject><subject>Erythroid Precursor Cells - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Luciferases - metabolism</subject><subject>Megakaryocytes - metabolism</subject><subject>Methylation</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Mutagenesis, Site-Directed</subject><subject>Plasmids</subject><subject>Platelet Membrane Glycoproteins - genetics</subject><subject>Promoter Regions, Genetic</subject><subject>Transcription, Genetic</subject><subject>Transcription. Transcription factor. Splicing. 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Psychology</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Luciferases - metabolism</topic><topic>Megakaryocytes - metabolism</topic><topic>Methylation</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Mutagenesis, Site-Directed</topic><topic>Plasmids</topic><topic>Platelet Membrane Glycoproteins - genetics</topic><topic>Promoter Regions, Genetic</topic><topic>Transcription, Genetic</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>PRANDINI, M.-H</creatorcontrib><creatorcontrib>UZAN, G</creatorcontrib><creatorcontrib>MARTIN, F</creatorcontrib><creatorcontrib>THEVENON, D</creatorcontrib><creatorcontrib>MARGUERIE, G</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>PRANDINI, M.-H</au><au>UZAN, G</au><au>MARTIN, F</au><au>THEVENON, D</au><au>MARGUERIE, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of a specific erythromegakaryocytic enhancer within the glycoprotein IIb promoter</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1992-05-25</date><risdate>1992</risdate><volume>267</volume><issue>15</issue><spage>10370</spage><epage>10374</epage><pages>10370-10374</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>The gene coding for glycoprotein IIb (GPIIb), the alpha subunit of platelet integrin GPIIb/IIIa is an early and specific marker
of the megakaryocytic lineage. Thus, studies on the regulation of this gene may provide helpful information on the mechanisms
controlling cell specificity and differentiation in this lineage. The promoter region of this gene was isolated and analyzed
to understand its tissue-specific transcriptional activity. A region extending from nucleotides -414 to -554 was found to
be extremely important for the promoter function. Deletion of this region results in a 70% decrease of the promoter activity,
as measured in CAT assays. This region has the properties of an enhancer. It is able to activate a heterologous promoter,
in a distance- and orientation-independent manner, in both megakaryocytic and erythroid cells. This enhancer contains binding
sites for nuclear factors and mutation of these sites, individually or together, abolish the enhancer activity. These nuclear
factors are present in megakaryocytic and erythroid cell lineages, but they are absent in the other tested cells. One of the
sites, named domain D, contains a TTATC motif that may interact with the transcription factor GATA1, active in erythroid and
megakaryocytic cells. These results indicate that the promoter of a megakaryocytic gene contains a tissue specific enhancer,
active in both the erythroid and the megakaryocytic lineages, and may implicate the erythroid factor GATA1.</abstract><cop>Bethesda, MD</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>1587823</pmid><doi>10.1016/s0021-9258(19)50028-x</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Base Sequence Biological and medical sciences Cell Line Chloramphenicol O-Acetyltransferase - metabolism Chromosome Deletion Electrophoresis, Polyacrylamide Gel Enhancer Elements, Genetic Erythroid Precursor Cells - metabolism Fundamental and applied biological sciences. Psychology HeLa Cells Humans Luciferases - metabolism Megakaryocytes - metabolism Methylation Molecular and cellular biology Molecular genetics Molecular Sequence Data Mutagenesis, Site-Directed Plasmids Platelet Membrane Glycoproteins - genetics Promoter Regions, Genetic Transcription, Genetic Transcription. Transcription factor. Splicing. Rna processing Transfection |
| title | Characterization of a specific erythromegakaryocytic enhancer within the glycoprotein IIb promoter |
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