Transformation of a grain legume (Lupinus angustifolius L.) via Agrobacterium tumefaciens-mediated gene transfer to shoot apices

Transgenic plants of Lupinus angustifolius L. (cvs. Unicrop and Merrit) were routinely generated using Agrobacterium-mediated gene transfer to shoot apices. The bar gene for resistance to phosphinothricin (PPT, the active ingredient of the herbicide Basta) was used as the selectable marker. After co...

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Veröffentlicht in:	Molecular breeding 1997-01, Vol.3 (5), p.341-349
Hauptverfasser:	Pigeaire, A, Abernethy, D, Smith, P.M, Simpson, K, Fletcher, N, Lu, C.Y, Atkins, C.A, Cornish, E
Format:	Artikel
Sprache:	eng
Schlagworte:	acyltransferases Agrobacterium Agrobacterium tumefaciens axillary shoots Bar gene basta beta-glucuronidase Cultivars Cultivation Explants Gene expression Gene transfer genetic markers genetic transformation Genomes glufosinate Greenhouses herbicide resistance Herbicides histochemistry in vitro selection inheritance (genetics) Legumes Lupinus angustifolius Molecular biology Phosphinothricin phosphinothricin acetyltransferase Plant biology Plant tissues Regeneration regenerative ability shoot apices Shoots Survival Transgenic plants
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