Bovine stem cell factor: production of a biologically active protein and mRNA analysis in cattle infected with Trypanosoma congolense
The cDNA coding for the soluble form of bovine stem cell factor (boSCF Ala 165 ) was cloned and recombinant protein was produced in bacteria as a histidine tagged-protein. The protein was purified from the inclusion bodies in one step by metal chelation chromatography under denaturing conditions. Re...
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| Veröffentlicht in: | Veterinary immunology and immunopathology 1997-10, Vol.59 (1), p.65-78 |
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| creator | Mertens, Bea Muriuki, Cecilia Muiya, Paul Andrianarivo, Aurelie Mwangi, Simon Logan-Henfrey, Linda |
| description | The cDNA coding for the soluble form of bovine stem cell factor (boSCF
Ala
165
) was cloned and recombinant protein was produced in bacteria as a histidine tagged-protein. The protein was purified from the inclusion bodies in one step by metal chelation chromatography under denaturing conditions. Recombinant bovine SCF was shown to act synergistically with interleukin 3 (IL-3) and erythropoietin (EPO) in stimulating the growth of bone marrow progenitor cells such as colony forming units-granulocyte macrophage (CFU-GM) and burst forming units-erythroid (BFU-E). Analysis of SCF mRNA expression by reverse transcription-polymerase chain reaction (RT-PCR) revealed that the transcripts were detectable in bone marrow, lymph node and spleen of cattle, and that the level of transcription was upregulated in lymph nodes of cattle infected with
Trypanosoma congolense. Two isoforms of SCF mRNA were amplified by RT-PCR. The availability of recombinant bovine SCF provides a valuable tool for studying the role of SCF in the development, growth and differentiation of bovine hematopoietic cells. |
| doi_str_mv | 10.1016/S0165-2427(97)00068-8 |
| format | Article |
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Ala
165
) was cloned and recombinant protein was produced in bacteria as a histidine tagged-protein. The protein was purified from the inclusion bodies in one step by metal chelation chromatography under denaturing conditions. Recombinant bovine SCF was shown to act synergistically with interleukin 3 (IL-3) and erythropoietin (EPO) in stimulating the growth of bone marrow progenitor cells such as colony forming units-granulocyte macrophage (CFU-GM) and burst forming units-erythroid (BFU-E). Analysis of SCF mRNA expression by reverse transcription-polymerase chain reaction (RT-PCR) revealed that the transcripts were detectable in bone marrow, lymph node and spleen of cattle, and that the level of transcription was upregulated in lymph nodes of cattle infected with
Trypanosoma congolense. Two isoforms of SCF mRNA were amplified by RT-PCR. The availability of recombinant bovine SCF provides a valuable tool for studying the role of SCF in the development, growth and differentiation of bovine hematopoietic cells.</description><identifier>ISSN: 0165-2427</identifier><identifier>EISSN: 1873-2534</identifier><identifier>DOI: 10.1016/S0165-2427(97)00068-8</identifier><identifier>PMID: 9437826</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>ADN ; Animals ; BONE MARROW ; Bone Marrow - drug effects ; BOVIN ; Bovine ; CATTLE ; Cattle Diseases - metabolism ; Cell Differentiation ; CELLS ; CELLULE ; CELULAS ; CLONACION ; CLONAGE ; CLONING ; Cloning, Molecular ; DNA ; DNA Primers - chemistry ; Drug Synergism ; ERITROCITOS ; ERYTHROCYTE ; ERYTHROCYTES ; Erythroid Precursor Cells - metabolism ; Erythropoietin - pharmacology ; Escherichia coli - genetics ; Escherichia coli - metabolism ; GANADO BOVINO ; Gene Expression ; GLICOPROTEINAS ; GLYCOPROTEINE ; GLYCOPROTEINS ; Granulocyte-Macrophage Colony-Stimulating Factor - genetics ; Granulocyte-Macrophage Colony-Stimulating Factor - metabolism ; HISTIDINA ; HISTIDINE ; Histidine tag ; Interleukin-3 - pharmacology ; INTERLEUKINE ; INTERLEUKINS ; INTERLEUQUINAS ; Lymph Nodes - metabolism ; MEDULA OSEA ; MOELLE OSSEUSE ; Polymerase Chain Reaction ; Recombinant Proteins - biosynthesis ; RNA, Messenger - analysis ; RT-PCR ; SCF ; Spleen - metabolism ; Stem cell factor ; Stem Cell Factor - biosynthesis ; Stem Cell Factor - genetics ; Stem Cell Factor - pharmacology ; TRYPANOSOMA CONGOLENSE ; Trypanosomiasis ; Trypanosomiasis, African - metabolism ; Trypanosomiasis, African - veterinary ; Up-Regulation</subject><ispartof>Veterinary immunology and immunopathology, 1997-10, Vol.59 (1), p.65-78</ispartof><rights>1997</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-c899a50b737023a0e72ba333ae7426c6e427a365f100f37769f22783791b26883</citedby><cites>FETCH-LOGICAL-c413t-c899a50b737023a0e72ba333ae7426c6e427a365f100f37769f22783791b26883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0165-2427(97)00068-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9437826$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mertens, Bea</creatorcontrib><creatorcontrib>Muriuki, Cecilia</creatorcontrib><creatorcontrib>Muiya, Paul</creatorcontrib><creatorcontrib>Andrianarivo, Aurelie</creatorcontrib><creatorcontrib>Mwangi, Simon</creatorcontrib><creatorcontrib>Logan-Henfrey, Linda</creatorcontrib><title>Bovine stem cell factor: production of a biologically active protein and mRNA analysis in cattle infected with Trypanosoma congolense</title><title>Veterinary immunology and immunopathology</title><addtitle>Vet Immunol Immunopathol</addtitle><description>The cDNA coding for the soluble form of bovine stem cell factor (boSCF
Ala
165
) was cloned and recombinant protein was produced in bacteria as a histidine tagged-protein. The protein was purified from the inclusion bodies in one step by metal chelation chromatography under denaturing conditions. Recombinant bovine SCF was shown to act synergistically with interleukin 3 (IL-3) and erythropoietin (EPO) in stimulating the growth of bone marrow progenitor cells such as colony forming units-granulocyte macrophage (CFU-GM) and burst forming units-erythroid (BFU-E). Analysis of SCF mRNA expression by reverse transcription-polymerase chain reaction (RT-PCR) revealed that the transcripts were detectable in bone marrow, lymph node and spleen of cattle, and that the level of transcription was upregulated in lymph nodes of cattle infected with
Trypanosoma congolense. Two isoforms of SCF mRNA were amplified by RT-PCR. The availability of recombinant bovine SCF provides a valuable tool for studying the role of SCF in the development, growth and differentiation of bovine hematopoietic cells.</description><subject>ADN</subject><subject>Animals</subject><subject>BONE MARROW</subject><subject>Bone Marrow - drug effects</subject><subject>BOVIN</subject><subject>Bovine</subject><subject>CATTLE</subject><subject>Cattle Diseases - metabolism</subject><subject>Cell Differentiation</subject><subject>CELLS</subject><subject>CELLULE</subject><subject>CELULAS</subject><subject>CLONACION</subject><subject>CLONAGE</subject><subject>CLONING</subject><subject>Cloning, Molecular</subject><subject>DNA</subject><subject>DNA Primers - chemistry</subject><subject>Drug Synergism</subject><subject>ERITROCITOS</subject><subject>ERYTHROCYTE</subject><subject>ERYTHROCYTES</subject><subject>Erythroid Precursor Cells - metabolism</subject><subject>Erythropoietin - pharmacology</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - metabolism</subject><subject>GANADO BOVINO</subject><subject>Gene Expression</subject><subject>GLICOPROTEINAS</subject><subject>GLYCOPROTEINE</subject><subject>GLYCOPROTEINS</subject><subject>Granulocyte-Macrophage Colony-Stimulating Factor - genetics</subject><subject>Granulocyte-Macrophage Colony-Stimulating Factor - metabolism</subject><subject>HISTIDINA</subject><subject>HISTIDINE</subject><subject>Histidine tag</subject><subject>Interleukin-3 - pharmacology</subject><subject>INTERLEUKINE</subject><subject>INTERLEUKINS</subject><subject>INTERLEUQUINAS</subject><subject>Lymph Nodes - metabolism</subject><subject>MEDULA OSEA</subject><subject>MOELLE OSSEUSE</subject><subject>Polymerase Chain Reaction</subject><subject>Recombinant Proteins - biosynthesis</subject><subject>RNA, Messenger - analysis</subject><subject>RT-PCR</subject><subject>SCF</subject><subject>Spleen - metabolism</subject><subject>Stem cell factor</subject><subject>Stem Cell Factor - biosynthesis</subject><subject>Stem Cell Factor - genetics</subject><subject>Stem Cell Factor - pharmacology</subject><subject>TRYPANOSOMA CONGOLENSE</subject><subject>Trypanosomiasis</subject><subject>Trypanosomiasis, African - metabolism</subject><subject>Trypanosomiasis, African - veterinary</subject><subject>Up-Regulation</subject><issn>0165-2427</issn><issn>1873-2534</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtu1DAUQC0EKtPCJ1TyCsEi4EfiBxtUqvKQRkWCsrYc53owSuLB9gyaD-C_cTqjsmRjX_ke38dB6JKS15RQ8eZbPbqGtUy-1PIVIUSoRj1CK6okb1jH28do9YA8Rec5_6xQp5U6Q2e65VIxsUJ_3sd9mAHnAhN2MI7YW1dieou3KQ47V0KccfTY4j7EMW6Cs-N4wJUJe1iYAmHGdh7w9PX2qgZ2POSQcX10tpQRauTBFRjw71B-4Lt02No55jhZ7OK8iSPMGZ6hJ96OGZ6f7gv0_cPN3fWnZv3l4-frq3XjWspL45TWtiO95JIwbglI1lvOuQXZMuEE1E0tF52nhHgupdCeMam41LRnQil-gV4c69bBf-0gFzOFvGxtZ4i7bKhgjHMmKtgdQZdizgm82aYw2XQwlJhFv7nXbxa3Rktzr98sDS5PDXb9BMPDr5Pvf3lvo7GbFLK5XVOtFSGMirbm3x3zUC3sAySTXYDZwRBStWiGGP4zwV_4154j</recordid><startdate>19971006</startdate><enddate>19971006</enddate><creator>Mertens, Bea</creator><creator>Muriuki, Cecilia</creator><creator>Muiya, Paul</creator><creator>Andrianarivo, Aurelie</creator><creator>Mwangi, Simon</creator><creator>Logan-Henfrey, Linda</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>19971006</creationdate><title>Bovine stem cell factor: production of a biologically active protein and mRNA analysis in cattle infected with Trypanosoma congolense</title><author>Mertens, Bea ; Muriuki, Cecilia ; Muiya, Paul ; Andrianarivo, Aurelie ; Mwangi, Simon ; Logan-Henfrey, Linda</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-c899a50b737023a0e72ba333ae7426c6e427a365f100f37769f22783791b26883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>ADN</topic><topic>Animals</topic><topic>BONE MARROW</topic><topic>Bone Marrow - drug effects</topic><topic>BOVIN</topic><topic>Bovine</topic><topic>CATTLE</topic><topic>Cattle Diseases - metabolism</topic><topic>Cell Differentiation</topic><topic>CELLS</topic><topic>CELLULE</topic><topic>CELULAS</topic><topic>CLONACION</topic><topic>CLONAGE</topic><topic>CLONING</topic><topic>Cloning, Molecular</topic><topic>DNA</topic><topic>DNA Primers - chemistry</topic><topic>Drug Synergism</topic><topic>ERITROCITOS</topic><topic>ERYTHROCYTE</topic><topic>ERYTHROCYTES</topic><topic>Erythroid Precursor Cells - metabolism</topic><topic>Erythropoietin - pharmacology</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - metabolism</topic><topic>GANADO BOVINO</topic><topic>Gene Expression</topic><topic>GLICOPROTEINAS</topic><topic>GLYCOPROTEINE</topic><topic>GLYCOPROTEINS</topic><topic>Granulocyte-Macrophage Colony-Stimulating Factor - genetics</topic><topic>Granulocyte-Macrophage Colony-Stimulating Factor - metabolism</topic><topic>HISTIDINA</topic><topic>HISTIDINE</topic><topic>Histidine tag</topic><topic>Interleukin-3 - pharmacology</topic><topic>INTERLEUKINE</topic><topic>INTERLEUKINS</topic><topic>INTERLEUQUINAS</topic><topic>Lymph Nodes - metabolism</topic><topic>MEDULA OSEA</topic><topic>MOELLE OSSEUSE</topic><topic>Polymerase Chain Reaction</topic><topic>Recombinant Proteins - biosynthesis</topic><topic>RNA, Messenger - analysis</topic><topic>RT-PCR</topic><topic>SCF</topic><topic>Spleen - metabolism</topic><topic>Stem cell factor</topic><topic>Stem Cell Factor - biosynthesis</topic><topic>Stem Cell Factor - genetics</topic><topic>Stem Cell Factor - pharmacology</topic><topic>TRYPANOSOMA CONGOLENSE</topic><topic>Trypanosomiasis</topic><topic>Trypanosomiasis, African - metabolism</topic><topic>Trypanosomiasis, African - veterinary</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mertens, Bea</creatorcontrib><creatorcontrib>Muriuki, Cecilia</creatorcontrib><creatorcontrib>Muiya, Paul</creatorcontrib><creatorcontrib>Andrianarivo, Aurelie</creatorcontrib><creatorcontrib>Mwangi, Simon</creatorcontrib><creatorcontrib>Logan-Henfrey, Linda</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Veterinary immunology and immunopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mertens, Bea</au><au>Muriuki, Cecilia</au><au>Muiya, Paul</au><au>Andrianarivo, Aurelie</au><au>Mwangi, Simon</au><au>Logan-Henfrey, Linda</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bovine stem cell factor: production of a biologically active protein and mRNA analysis in cattle infected with Trypanosoma congolense</atitle><jtitle>Veterinary immunology and immunopathology</jtitle><addtitle>Vet Immunol Immunopathol</addtitle><date>1997-10-06</date><risdate>1997</risdate><volume>59</volume><issue>1</issue><spage>65</spage><epage>78</epage><pages>65-78</pages><issn>0165-2427</issn><eissn>1873-2534</eissn><abstract>The cDNA coding for the soluble form of bovine stem cell factor (boSCF
Ala
165
) was cloned and recombinant protein was produced in bacteria as a histidine tagged-protein. The protein was purified from the inclusion bodies in one step by metal chelation chromatography under denaturing conditions. Recombinant bovine SCF was shown to act synergistically with interleukin 3 (IL-3) and erythropoietin (EPO) in stimulating the growth of bone marrow progenitor cells such as colony forming units-granulocyte macrophage (CFU-GM) and burst forming units-erythroid (BFU-E). Analysis of SCF mRNA expression by reverse transcription-polymerase chain reaction (RT-PCR) revealed that the transcripts were detectable in bone marrow, lymph node and spleen of cattle, and that the level of transcription was upregulated in lymph nodes of cattle infected with
Trypanosoma congolense. Two isoforms of SCF mRNA were amplified by RT-PCR. The availability of recombinant bovine SCF provides a valuable tool for studying the role of SCF in the development, growth and differentiation of bovine hematopoietic cells.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>9437826</pmid><doi>10.1016/S0165-2427(97)00068-8</doi><tpages>14</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0165-2427 |
| ispartof | Veterinary immunology and immunopathology, 1997-10, Vol.59 (1), p.65-78 |
| issn | 0165-2427 1873-2534 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16223326 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | ADN Animals BONE MARROW Bone Marrow - drug effects BOVIN Bovine CATTLE Cattle Diseases - metabolism Cell Differentiation CELLS CELLULE CELULAS CLONACION CLONAGE CLONING Cloning, Molecular DNA DNA Primers - chemistry Drug Synergism ERITROCITOS ERYTHROCYTE ERYTHROCYTES Erythroid Precursor Cells - metabolism Erythropoietin - pharmacology Escherichia coli - genetics Escherichia coli - metabolism GANADO BOVINO Gene Expression GLICOPROTEINAS GLYCOPROTEINE GLYCOPROTEINS Granulocyte-Macrophage Colony-Stimulating Factor - genetics Granulocyte-Macrophage Colony-Stimulating Factor - metabolism HISTIDINA HISTIDINE Histidine tag Interleukin-3 - pharmacology INTERLEUKINE INTERLEUKINS INTERLEUQUINAS Lymph Nodes - metabolism MEDULA OSEA MOELLE OSSEUSE Polymerase Chain Reaction Recombinant Proteins - biosynthesis RNA, Messenger - analysis RT-PCR SCF Spleen - metabolism Stem cell factor Stem Cell Factor - biosynthesis Stem Cell Factor - genetics Stem Cell Factor - pharmacology TRYPANOSOMA CONGOLENSE Trypanosomiasis Trypanosomiasis, African - metabolism Trypanosomiasis, African - veterinary Up-Regulation |
| title | Bovine stem cell factor: production of a biologically active protein and mRNA analysis in cattle infected with Trypanosoma congolense |
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