Purifications and properties of orotidine-phosphorolyzing enzyme and purine nucleoside phosphorylase from Erwinia carotovora AJ 2992

An orotidine-phosphorolyzing enzyme and a purine nucleoside phosphorylase (PNPase) of Erwinia carotovora AJ 2992, which is a potent producer of ribavirin (1-β-d-ribofuranosyl-1,2,4-triazole-3-carboxamide), an antiviral agent, from orotidine and 1,2,4-triazole-3-carboxamide (TCA), were purified 23-fo...

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Veröffentlicht in:	Agricultural and biological chemistry 1991-07, Vol.55 (7), p.1849-1857
Hauptverfasser:	Shirae, H. (Ajinomoto Co. Inc., Kawasaki, Kanagawa (Japan). Central Research Labs.), Yokozeki, K
Format:	Artikel
Sprache:	eng
Schlagworte:	ACIDE OROTIQUE ACIDO OROTICO Amino Acid Sequence Biological and medical sciences Biotechnology CHEMICOPHYSICAL PROPERTIES ENZIMAS ENZYME Enzyme engineering ENZYMES ERWINIA CAROTOVORA FOSFOPIROLASA FOSFORILACION Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Isoelectric Point Kinetics Metals - pharmacology Methods. Procedures. Technologies Molecular Sequence Data Molecular Weight NUCLEOSIDE nucleoside phosphorylase NUCLEOSIDES NUCLEOSIDOS OROTIC ACID orotidine Osmolar Concentration Pectobacterium carotovorum - enzymology Phosphates - pharmacology PHOSPHORYLASE PHOSPHORYLATION PROPIEDADES FISICO-QUIMICAS PROPRIETE PHYSICOCHIMIQUE PURIFICACION PURIFICATION PURINAS PURINE Purine-Nucleoside Phosphorylase - antagonists & inhibitors Purine-Nucleoside Phosphorylase - isolation & purification Purine-Nucleoside Phosphorylase - metabolism PURINES Ribavirin - metabolism RIBONUCLEOSIDE RIBONUCLEOSIDES RIBONUCLEOSIDOS Substrate Specificity Temperature Uridine Phosphorylase - isolation & purification Uridine Phosphorylase - metabolism
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