Cu and Cd Effects on the Earthworm Lumbricus rubellus in the Laboratory: Multivariate Statistical Analysis of Relationships between Exposure, Biomarkers, and Ecologically Relevant Parameters
This study sets out to examine the potential of a suite of novel molecular biomarkers as early warning indicators of environmental state and damage. Transcriptional responses of four genes, metallothionein 1 and 2, amine oxidase, and the lysosomal associated glycoprotein, were measured in the earthw...
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| Veröffentlicht in: | Environmental science & technology 2005-03, Vol.39 (6), p.1757-1763 |
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| creator | Burgos, Malyka Galay Winters, Carole Stürzenbaum, Stephen R Randerson, Peter F Kille, Peter Morgan, A. John |
| description | This study sets out to examine the potential of a suite of novel molecular biomarkers as early warning indicators of environmental state and damage. Transcriptional responses of four genes, metallothionein 1 and 2, amine oxidase, and the lysosomal associated glycoprotein, were measured in the earthworm Lumbricus rubellus exposed to increasing concentrations of cadmium and copper in OECD soil. These responses were compared to metal body concentra tions and lifecycle parameters: survival, cocoon production, and growth. Adverse physiological effects were observed at concentrations 1/3rd to 1/10th those of the artificial soil LC50. Multivariate statistics, principal component analysis (PCA), was used to investigate the correlations between the different variables. Three key components were derived explaining 77.6% of the variance, with component 1 contributing 32.4%, component 2 contributing 26.7%, and component 3 contributing 18.5%. These components were interpreted in terms of population health, pollutant exposure, and detoxification pathways, respectively. It is proposed that the use of such a suite of biomarkers could serve as indicators of the “health” of the soil environment and provide early warning signals of potential danger to the biota or as a means of monitoring soil remediation. |
| doi_str_mv | 10.1021/es049174x |
| format | Article |
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Three key components were derived explaining 77.6% of the variance, with component 1 contributing 32.4%, component 2 contributing 26.7%, and component 3 contributing 18.5%. These components were interpreted in terms of population health, pollutant exposure, and detoxification pathways, respectively. It is proposed that the use of such a suite of biomarkers could serve as indicators of the “health” of the soil environment and provide early warning signals of potential danger to the biota or as a means of monitoring soil remediation.</description><identifier>ISSN: 0013-936X</identifier><identifier>EISSN: 1520-5851</identifier><identifier>DOI: 10.1021/es049174x</identifier><identifier>PMID: 15819235</identifier><identifier>CODEN: ESTHAG</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Animal, plant and microbial ecology ; Animals ; Applied ecology ; Biological and medical sciences ; Biomarkers ; Biomarkers - analysis ; Cadmium - toxicity ; Chemical elements ; Copper ; Copper - toxicity ; Ecotoxicology, biological effects of pollution ; Effects of pollution and side effects of pesticides on protozoa and invertebrates ; Environmental Exposure ; Fundamental and applied biological sciences. 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John</creatorcontrib><title>Cu and Cd Effects on the Earthworm Lumbricus rubellus in the Laboratory: Multivariate Statistical Analysis of Relationships between Exposure, Biomarkers, and Ecologically Relevant Parameters</title><title>Environmental science & technology</title><addtitle>Environ. Sci. Technol</addtitle><description>This study sets out to examine the potential of a suite of novel molecular biomarkers as early warning indicators of environmental state and damage. Transcriptional responses of four genes, metallothionein 1 and 2, amine oxidase, and the lysosomal associated glycoprotein, were measured in the earthworm Lumbricus rubellus exposed to increasing concentrations of cadmium and copper in OECD soil. These responses were compared to metal body concentra tions and lifecycle parameters: survival, cocoon production, and growth. Adverse physiological effects were observed at concentrations 1/3rd to 1/10th those of the artificial soil LC50. Multivariate statistics, principal component analysis (PCA), was used to investigate the correlations between the different variables. Three key components were derived explaining 77.6% of the variance, with component 1 contributing 32.4%, component 2 contributing 26.7%, and component 3 contributing 18.5%. These components were interpreted in terms of population health, pollutant exposure, and detoxification pathways, respectively. 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Psychology</subject><subject>Genes</subject><subject>Lumbricus rubellus</subject><subject>Multivariate Analysis</subject><subject>Oligochaeta - physiology</subject><subject>Principal components analysis</subject><subject>Reproduction</subject><subject>Soil contamination</subject><subject>Soil Pollutants - toxicity</subject><subject>Survival</subject><subject>Tissue Distribution</subject><subject>Worms</subject><issn>0013-936X</issn><issn>1520-5851</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNplkc1uEzEUhUcIRENhwQsgCwkkpA7YY3t-2LXRUECJqEiRurM8nmviMjMOtqdNdmx5LF6FJ8EhUSPBypbud4_uOSdJnhL8muCMvAGPWUUKtr6XTAjPcMpLTu4nE4wJTSuaXx0lj7y_xhhnFJcPkyPCS1JllE-SX9MRyaFF0xbVWoMKHtkBhSWgWrqwvLWuR7Oxb5xRo0dubKDr4sfsmJlsrJPBus3b3z9-ovnYBXMjnZEB0CLIYHwwSnbodJDdxpuordFn6OLADn5pVh41EG4BBlSvV9aPDk7QmbG9dN_A-ZO_l9XKdvbrVqbbbJfhRg4BXUgnewiRepw80LLz8GT_Hidf3tWX0_fp7NP5h-npLJWMViElbQPAcZ5rRXOVESBcV5owlTPO2kZLVnKVlbwB2rZQMFrwqpCZ1pxXJaEFPU5e7nRXzn4fwQfRG69iHHIAO3pB8gyXLCsj-Pwf8NqOLibgRYw_SpWYRujVDlLOeu9Ai5Uz0fdGECy2pYq7UiP7bC84Nj20B3LfYgRe7AHpY07ayUEZf-DynFYMs8ilOy72Auu7eUxb5EU0LC4vFuLj_JxU86szsTjoSuUPJv4_8A_y7MlP</recordid><startdate>20050315</startdate><enddate>20050315</enddate><creator>Burgos, Malyka Galay</creator><creator>Winters, Carole</creator><creator>Stürzenbaum, Stephen R</creator><creator>Randerson, Peter F</creator><creator>Kille, Peter</creator><creator>Morgan, A. John</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7ST</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope><scope>7TV</scope></search><sort><creationdate>20050315</creationdate><title>Cu and Cd Effects on the Earthworm Lumbricus rubellus in the Laboratory: Multivariate Statistical Analysis of Relationships between Exposure, Biomarkers, and Ecologically Relevant Parameters</title><author>Burgos, Malyka Galay ; Winters, Carole ; Stürzenbaum, Stephen R ; Randerson, Peter F ; Kille, Peter ; Morgan, A. John</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a439t-1dbee5066fc36c21e15f9f14c6454dbfa485c285be3dde7437597a2ff55981373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animal, plant and microbial ecology</topic><topic>Animals</topic><topic>Applied ecology</topic><topic>Biological and medical sciences</topic><topic>Biomarkers</topic><topic>Biomarkers - analysis</topic><topic>Cadmium - toxicity</topic><topic>Chemical elements</topic><topic>Copper</topic><topic>Copper - toxicity</topic><topic>Ecotoxicology, biological effects of pollution</topic><topic>Effects of pollution and side effects of pesticides on protozoa and invertebrates</topic><topic>Environmental Exposure</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Lumbricus rubellus</topic><topic>Multivariate Analysis</topic><topic>Oligochaeta - physiology</topic><topic>Principal components analysis</topic><topic>Reproduction</topic><topic>Soil contamination</topic><topic>Soil Pollutants - toxicity</topic><topic>Survival</topic><topic>Tissue Distribution</topic><topic>Worms</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Burgos, Malyka Galay</creatorcontrib><creatorcontrib>Winters, Carole</creatorcontrib><creatorcontrib>Stürzenbaum, Stephen R</creatorcontrib><creatorcontrib>Randerson, Peter F</creatorcontrib><creatorcontrib>Kille, Peter</creatorcontrib><creatorcontrib>Morgan, A. 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John</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cu and Cd Effects on the Earthworm Lumbricus rubellus in the Laboratory: Multivariate Statistical Analysis of Relationships between Exposure, Biomarkers, and Ecologically Relevant Parameters</atitle><jtitle>Environmental science & technology</jtitle><addtitle>Environ. Sci. Technol</addtitle><date>2005-03-15</date><risdate>2005</risdate><volume>39</volume><issue>6</issue><spage>1757</spage><epage>1763</epage><pages>1757-1763</pages><issn>0013-936X</issn><eissn>1520-5851</eissn><coden>ESTHAG</coden><abstract>This study sets out to examine the potential of a suite of novel molecular biomarkers as early warning indicators of environmental state and damage. Transcriptional responses of four genes, metallothionein 1 and 2, amine oxidase, and the lysosomal associated glycoprotein, were measured in the earthworm Lumbricus rubellus exposed to increasing concentrations of cadmium and copper in OECD soil. These responses were compared to metal body concentra tions and lifecycle parameters: survival, cocoon production, and growth. Adverse physiological effects were observed at concentrations 1/3rd to 1/10th those of the artificial soil LC50. Multivariate statistics, principal component analysis (PCA), was used to investigate the correlations between the different variables. Three key components were derived explaining 77.6% of the variance, with component 1 contributing 32.4%, component 2 contributing 26.7%, and component 3 contributing 18.5%. These components were interpreted in terms of population health, pollutant exposure, and detoxification pathways, respectively. 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| identifier | ISSN: 0013-936X |
| ispartof | Environmental science & technology, 2005-03, Vol.39 (6), p.1757-1763 |
| issn | 0013-936X 1520-5851 |
| language | eng |
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| source | MEDLINE; ACS Publications |
| subjects | Animal, plant and microbial ecology Animals Applied ecology Biological and medical sciences Biomarkers Biomarkers - analysis Cadmium - toxicity Chemical elements Copper Copper - toxicity Ecotoxicology, biological effects of pollution Effects of pollution and side effects of pesticides on protozoa and invertebrates Environmental Exposure Fundamental and applied biological sciences. Psychology Genes Lumbricus rubellus Multivariate Analysis Oligochaeta - physiology Principal components analysis Reproduction Soil contamination Soil Pollutants - toxicity Survival Tissue Distribution Worms |
| title | Cu and Cd Effects on the Earthworm Lumbricus rubellus in the Laboratory: Multivariate Statistical Analysis of Relationships between Exposure, Biomarkers, and Ecologically Relevant Parameters |
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