HPLC determination of cefprozil in tablets using monolithic and C18 silica columns
Cefprozil (CPZ) is a second‐generation semi‐synthetic cephalosporin antibiotic that commonly exists as the mixture of Z and E diastereoisomers, at the ratio of approximately 9:1. A novel reversed‐phase HPLC method for the determination of CPZ in tablets was described. The separation of CPZ diastereo...
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| Veröffentlicht in: | Journal of separation science 2011-08, Vol.34 (16-17), p.2223-2231 |
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| container_title | Journal of separation science |
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| creator | Can, Nafiz O. |
| description | Cefprozil (CPZ) is a second‐generation semi‐synthetic cephalosporin antibiotic that commonly exists as the mixture of Z and E diastereoisomers, at the ratio of approximately 9:1. A novel reversed‐phase HPLC method for the determination of CPZ in tablets was described. The separation of CPZ diastereoisomers and caffeine (internal standard) was carried out by applying the same analytical and instrumental conditions on two stationary phases, which have different surface chemistries. The columns used in the study were monolithic silica Merck Chromolith Performance RP‐18e and conventional C18 silica Phenomenex Synergi Hydro RP columns. In total, 10 μL aliquots of samples were injected into the system and eluted using water–acetonitrile (90:10, v/v) solution, which was pumped through the column at a flow rate of 1.0 mL/min. The analyte peaks were detected at 200 nm using diode array detector with high specificity. CPZ diastereoisomers and caffeine were measured within 13 min using the C18 column, whereas |
| doi_str_mv | 10.1002/jssc.201100170 |
| format | Article |
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A novel reversed‐phase HPLC method for the determination of CPZ in tablets was described. The separation of CPZ diastereoisomers and caffeine (internal standard) was carried out by applying the same analytical and instrumental conditions on two stationary phases, which have different surface chemistries. The columns used in the study were monolithic silica Merck Chromolith Performance RP‐18e and conventional C18 silica Phenomenex Synergi Hydro RP columns. In total, 10 μL aliquots of samples were injected into the system and eluted using water–acetonitrile (90:10, v/v) solution, which was pumped through the column at a flow rate of 1.0 mL/min. The analyte peaks were detected at 200 nm using diode array detector with high specificity. CPZ diastereoisomers and caffeine were measured within 13 min using the C18 column, whereas <5 min was required for the monolithic one. Validation studies were performed according to official recommendations. Value of a monolithic column for the assay of diastereoisomers in pharmaceutical tablets was evaluated for the first time and found as a powerful alternative to highly efficient C18 columns.</description><identifier>ISSN: 1615-9306</identifier><identifier>ISSN: 1615-9314</identifier><identifier>EISSN: 1615-9314</identifier><identifier>DOI: 10.1002/jssc.201100170</identifier><identifier>PMID: 21557474</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>Anti-Bacterial Agents - analysis ; Arrays ; Caffeine ; Cefprozil ; Cephalosporins - analysis ; Chromatography, High Pressure Liquid - instrumentation ; Chromatography, High Pressure Liquid - methods ; Diastereoisomer separation ; Diodes ; Mathematical analysis ; Monolithic silica column ; Pharmaceutical application ; Phases ; Separation ; Silicon dioxide ; Tablets ; Tablets - analysis</subject><ispartof>Journal of separation science, 2011-08, Vol.34 (16-17), p.2223-2231</ispartof><rights>Copyright © 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><rights>Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjssc.201100170$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjssc.201100170$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21557474$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Can, Nafiz O.</creatorcontrib><title>HPLC determination of cefprozil in tablets using monolithic and C18 silica columns</title><title>Journal of separation science</title><addtitle>J. Sep. Science</addtitle><description>Cefprozil (CPZ) is a second‐generation semi‐synthetic cephalosporin antibiotic that commonly exists as the mixture of Z and E diastereoisomers, at the ratio of approximately 9:1. A novel reversed‐phase HPLC method for the determination of CPZ in tablets was described. The separation of CPZ diastereoisomers and caffeine (internal standard) was carried out by applying the same analytical and instrumental conditions on two stationary phases, which have different surface chemistries. The columns used in the study were monolithic silica Merck Chromolith Performance RP‐18e and conventional C18 silica Phenomenex Synergi Hydro RP columns. In total, 10 μL aliquots of samples were injected into the system and eluted using water–acetonitrile (90:10, v/v) solution, which was pumped through the column at a flow rate of 1.0 mL/min. The analyte peaks were detected at 200 nm using diode array detector with high specificity. CPZ diastereoisomers and caffeine were measured within 13 min using the C18 column, whereas <5 min was required for the monolithic one. Validation studies were performed according to official recommendations. Value of a monolithic column for the assay of diastereoisomers in pharmaceutical tablets was evaluated for the first time and found as a powerful alternative to highly efficient C18 columns.</description><subject>Anti-Bacterial Agents - analysis</subject><subject>Arrays</subject><subject>Caffeine</subject><subject>Cefprozil</subject><subject>Cephalosporins - analysis</subject><subject>Chromatography, High Pressure Liquid - instrumentation</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Diastereoisomer separation</subject><subject>Diodes</subject><subject>Mathematical analysis</subject><subject>Monolithic silica column</subject><subject>Pharmaceutical application</subject><subject>Phases</subject><subject>Separation</subject><subject>Silicon dioxide</subject><subject>Tablets</subject><subject>Tablets - analysis</subject><issn>1615-9306</issn><issn>1615-9314</issn><issn>1615-9314</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9P3TAMxyO0CRhw3XGKtAuXgp00aXKcqgGbnhiMHztGaZtueUtT1rTa4K9fnx7rgctOtuXP17L9JeQtwgkCsNN1SvUJA5wLLGCH7KNEkWmO-aslB7lH3qS03iBKwy7ZYyhEkRf5Pvl6cbUqaeNGN3Q-2tH3kfYtrV37MPRPPlAf6Wir4MZEp-Tjd9r1sQ9-_OFramNDS1Q0-eBrS-s-TF1Mh-R1a0NyR8_xgNydfbwtL7LVl_NP5YdV5hkIyBoncgnMyaYSra1QC1E5JXSjQFnBnaxyqYA3wFnB85Yj1MrpSjhdM9tyzQ_I8XbuvOmvyaXRdD7VLgQbXT8lg5LNHxIK1f9R4Mg0kwAz-v4Fuu6nIc6HGBS5AF0Ums_Uu2dqqjrXmIfBd3Z4NP8eOwN6C_z2wT0ufQSzsc1sbDOLbebzzU25VLM222p9Gt2fRWuHn0YWvBDm2-W5ub8uyyuO1-aW_wUONZgk</recordid><startdate>201108</startdate><enddate>201108</enddate><creator>Can, Nafiz O.</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>201108</creationdate><title>HPLC determination of cefprozil in tablets using monolithic and C18 silica columns</title><author>Can, Nafiz O.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i2050-de54602e6db5fab1955be859d808a53e6b46803d032734f310c8e9b5e9c2af393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Anti-Bacterial Agents - analysis</topic><topic>Arrays</topic><topic>Caffeine</topic><topic>Cefprozil</topic><topic>Cephalosporins - analysis</topic><topic>Chromatography, High Pressure Liquid - instrumentation</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Diastereoisomer separation</topic><topic>Diodes</topic><topic>Mathematical analysis</topic><topic>Monolithic silica column</topic><topic>Pharmaceutical application</topic><topic>Phases</topic><topic>Separation</topic><topic>Silicon dioxide</topic><topic>Tablets</topic><topic>Tablets - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Can, Nafiz O.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of separation science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Can, Nafiz O.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>HPLC determination of cefprozil in tablets using monolithic and C18 silica columns</atitle><jtitle>Journal of separation science</jtitle><addtitle>J. Sep. Science</addtitle><date>2011-08</date><risdate>2011</risdate><volume>34</volume><issue>16-17</issue><spage>2223</spage><epage>2231</epage><pages>2223-2231</pages><issn>1615-9306</issn><issn>1615-9314</issn><eissn>1615-9314</eissn><abstract>Cefprozil (CPZ) is a second‐generation semi‐synthetic cephalosporin antibiotic that commonly exists as the mixture of Z and E diastereoisomers, at the ratio of approximately 9:1. A novel reversed‐phase HPLC method for the determination of CPZ in tablets was described. The separation of CPZ diastereoisomers and caffeine (internal standard) was carried out by applying the same analytical and instrumental conditions on two stationary phases, which have different surface chemistries. The columns used in the study were monolithic silica Merck Chromolith Performance RP‐18e and conventional C18 silica Phenomenex Synergi Hydro RP columns. In total, 10 μL aliquots of samples were injected into the system and eluted using water–acetonitrile (90:10, v/v) solution, which was pumped through the column at a flow rate of 1.0 mL/min. The analyte peaks were detected at 200 nm using diode array detector with high specificity. CPZ diastereoisomers and caffeine were measured within 13 min using the C18 column, whereas <5 min was required for the monolithic one. Validation studies were performed according to official recommendations. Value of a monolithic column for the assay of diastereoisomers in pharmaceutical tablets was evaluated for the first time and found as a powerful alternative to highly efficient C18 columns.</abstract><cop>Weinheim</cop><pub>WILEY-VCH Verlag</pub><pmid>21557474</pmid><doi>10.1002/jssc.201100170</doi><tpages>9</tpages></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Anti-Bacterial Agents - analysis Arrays Caffeine Cefprozil Cephalosporins - analysis Chromatography, High Pressure Liquid - instrumentation Chromatography, High Pressure Liquid - methods Diastereoisomer separation Diodes Mathematical analysis Monolithic silica column Pharmaceutical application Phases Separation Silicon dioxide Tablets Tablets - analysis |
| title | HPLC determination of cefprozil in tablets using monolithic and C18 silica columns |
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