Use of eukaryotic mitochondrial DNA to differentiate human, bovine, porcine and ovine sources in fecally contaminated surface water
A molecular method based on the detection of mitochondrial DNA from various animal species was developed to track the origin of surface water pollutions, and to differentiate human and animal sources. Mitochondrial DNA sequences were used to design PCR primers specific for human, bovine, ovine and p...
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| Veröffentlicht in: | Water research (Oxford) 2005-02, Vol.39 (4), p.541-548 |
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| creator | Martellini, Anouk Payment, Pierre Villemur, Richard |
| description | A molecular method based on the detection of mitochondrial DNA from various animal species was developed to track the origin of surface water pollutions, and to differentiate human and animal sources. Mitochondrial DNA sequences were used to design PCR primers specific for human, bovine, ovine and porcine DNA using single, multiplex and nested PCR protocols. The primers were tested with DNA extracted from untreated domestic sewage, agricultural soils run-off, swine farm effluents and water from two rivers with known pollution sources. At least one of the four species was detected in most of these samples. The limit of detection in wastewater was 10
3–10
4 cells
L
−1 with a multiplex PCR protocol. This is the first report of a method using eukaryotic genetic DNA to detect and differentiate animal DNA from fecal sources in water. This innovative method is simple and could be used to quickly differentiate sources of pollution in a watershed. |
| doi_str_mv | 10.1016/j.watres.2004.11.012 |
| format | Article |
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3–10
4 cells
L
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3–10
4 cells
L
−1 with a multiplex PCR protocol. This is the first report of a method using eukaryotic genetic DNA to detect and differentiate animal DNA from fecal sources in water. This innovative method is simple and could be used to quickly differentiate sources of pollution in a watershed.</description><subject>Agriculture</subject><subject>Animal source tracking</subject><subject>Animals</subject><subject>Applied sciences</subject><subject>Base Sequence</subject><subject>Cattle</subject><subject>contaminants</subject><subject>Continental surface waters</subject><subject>DNA, Mitochondrial - analysis</subject><subject>Earth sciences</subject><subject>Earth, ocean, space</subject><subject>Engineering and environment geology. Geothermics</subject><subject>Eukaryotic Cells</subject><subject>Exact sciences and technology</subject><subject>Fecal pollution</subject><subject>feces</subject><subject>Feces - virology</subject><subject>genetic markers</subject><subject>Humans</subject><subject>Mitochondrial DNA</subject><subject>Natural water pollution</subject><subject>Pollution</subject><subject>Pollution, environment geology</subject><subject>polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Rivers - microbiology</subject><subject>Sewage - microbiology</subject><subject>sheep</subject><subject>Sheep, Domestic</subject><subject>Soil - analysis</subject><subject>Species Specificity</subject><subject>Surface water</subject><subject>Swine</subject><subject>Wastewater</subject><subject>Water Pollution</subject><subject>Water treatment and pollution</subject><subject>Watershed</subject><issn>0043-1354</issn><issn>1879-2448</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxSMEotvCN0DgC5y6i8dxnOSCVJXyR6rgAHu2Zu0x9ZLYWzsp6pkvjpddqTc4jWX_3tP4vap6AXwFHNTb7eoXTonySnAuVwArDuJRtYCu7ZdCyu5xtSgP9RLqRp5UpzlvOedC1P3T6gSalrdKqEX1e52JRcdo_onpPk7esNFP0dzEYJPHgb3_csGmyKx3jhKFyeNE7GYeMZyzTbzzgc7ZLiZTDgyDZX-vWI5zMpSZD8yRwWG4ZyaGCUcfit6yPCeHhlj5AqVn1ROHQ6bnx3lWrT9cfb_8tLz--vHz5cX10jRSTMtOdA2AaKzt0CoiBN7VLZIsszUtAVrRoeqNcptaYO96s3GtVCSFwJJKfVa9OfjuUrydKU969NnQMGCgOGcNCnqoZfd_ULY1lCgLKA-gSTHnRE7vkh9LkBq43rekt_rQkt63pAF0aanIXh79581I9kF0rKUAr48A5pKeSxiMzw-caoRU9d7o1YFzGDX-SIVZfxMcas77DhTfO707EFSCvfOUdDaegiHrE5lJ2-j_vesfnQy9Dw</recordid><startdate>20050201</startdate><enddate>20050201</enddate><creator>Martellini, Anouk</creator><creator>Payment, Pierre</creator><creator>Villemur, Richard</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>C1K</scope><scope>SOI</scope><scope>7QH</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7TV</scope><scope>7UA</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H96</scope><scope>L.G</scope><scope>P64</scope></search><sort><creationdate>20050201</creationdate><title>Use of eukaryotic mitochondrial DNA to differentiate human, bovine, porcine and ovine sources in fecally contaminated surface water</title><author>Martellini, Anouk ; Payment, Pierre ; Villemur, Richard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c542t-82851125dd8ad6eea10837ae41087c7e1ad28a69c6fb32a9f9cbf746e422a8793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Agriculture</topic><topic>Animal source tracking</topic><topic>Animals</topic><topic>Applied sciences</topic><topic>Base Sequence</topic><topic>Cattle</topic><topic>contaminants</topic><topic>Continental surface waters</topic><topic>DNA, Mitochondrial - analysis</topic><topic>Earth sciences</topic><topic>Earth, ocean, space</topic><topic>Engineering and environment geology. 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Mitochondrial DNA sequences were used to design PCR primers specific for human, bovine, ovine and porcine DNA using single, multiplex and nested PCR protocols. The primers were tested with DNA extracted from untreated domestic sewage, agricultural soils run-off, swine farm effluents and water from two rivers with known pollution sources. At least one of the four species was detected in most of these samples. The limit of detection in wastewater was 10
3–10
4 cells
L
−1 with a multiplex PCR protocol. This is the first report of a method using eukaryotic genetic DNA to detect and differentiate animal DNA from fecal sources in water. This innovative method is simple and could be used to quickly differentiate sources of pollution in a watershed.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>15707626</pmid><doi>10.1016/j.watres.2004.11.012</doi><tpages>8</tpages></addata></record> |
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| subjects | Agriculture Animal source tracking Animals Applied sciences Base Sequence Cattle contaminants Continental surface waters DNA, Mitochondrial - analysis Earth sciences Earth, ocean, space Engineering and environment geology. Geothermics Eukaryotic Cells Exact sciences and technology Fecal pollution feces Feces - virology genetic markers Humans Mitochondrial DNA Natural water pollution Pollution Pollution, environment geology polymerase chain reaction Polymerase Chain Reaction - methods Rivers - microbiology Sewage - microbiology sheep Sheep, Domestic Soil - analysis Species Specificity Surface water Swine Wastewater Water Pollution Water treatment and pollution Watershed |
| title | Use of eukaryotic mitochondrial DNA to differentiate human, bovine, porcine and ovine sources in fecally contaminated surface water |
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