The interaction of κ-bungarotoxin with the nicotinic receptor of bovine chromaffin cells
Whole-cell recording techniques were used to examine acetylcholine-induced nicotinic currents in isolated bovinei chromaffin cells. The effects on these currents of κ-bungarotoxin, a snake venon κ-neurotoxin, were tested. Exposure of cells to κ-bungarotoxin (600 nM for 40 min) produced a prolonged b...
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| Veröffentlicht in: | Brain research 1992-02, Vol.573 (1), p.77-82 |
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| creator | Nooney, Janet M. Lambert, Jeremy J. Chiappinelli, Vincent A. |
| description | Whole-cell recording techniques were used to examine acetylcholine-induced nicotinic currents in isolated bovinei chromaffin cells. The effects on these currents of κ-bungarotoxin, a snake venon κ-neurotoxin, were tested. Exposure of cells to κ-bungarotoxin (600 nM for 40 min) produced a prolonged blockade of nicotinic currents. The mechanism of this blockade was examined in several ways. Firstly, the pre-exposure of cells to trimetaphan, a competitive nicotinic antagonist, protected against the action of subsequent additions of κ-bungarotoxin. Secondly, voltage-clamp measurements indicated that the degree of blockade produced by κ-bungarotoxin was independent of cell membrane potential. Unlike (+)-tubocurarine, κ-bungarotoxin had no direct agonist effects on nicotinic receptors. It is concluded from the present functional studies and from previously reported binding studies that κ-bungarotoxin blocks nicotinic responses in bovine chromaffin cells by binding to regions overlying acetylcholine sites on nicotinic receptors. |
| doi_str_mv | 10.1016/0006-8993(92)90115-P |
| format | Article |
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The effects on these currents of κ-bungarotoxin, a snake venon κ-neurotoxin, were tested. Exposure of cells to κ-bungarotoxin (600 nM for 40 min) produced a prolonged blockade of nicotinic currents. The mechanism of this blockade was examined in several ways. Firstly, the pre-exposure of cells to trimetaphan, a competitive nicotinic antagonist, protected against the action of subsequent additions of κ-bungarotoxin. Secondly, voltage-clamp measurements indicated that the degree of blockade produced by κ-bungarotoxin was independent of cell membrane potential. Unlike (+)-tubocurarine, κ-bungarotoxin had no direct agonist effects on nicotinic receptors. It is concluded from the present functional studies and from previously reported binding studies that κ-bungarotoxin blocks nicotinic responses in bovine chromaffin cells by binding to regions overlying acetylcholine sites on nicotinic receptors.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/0006-8993(92)90115-P</identifier><identifier>PMID: 1576536</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Acetylcholine ; Acetylcholine - pharmacology ; Animals ; Biological and medical sciences ; Bovine adrenomedullary chromaffin cell ; Bungarotoxins - pharmacology ; Cattle ; Cell receptors ; Cell structures and functions ; Cells, Cultured ; Enterochromaffin Cells - drug effects ; Enterochromaffin Cells - metabolism ; Fundamental and applied biological sciences. Psychology ; Ganglionic Stimulants - pharmacology ; Molecular and cellular biology ; Monoamines receptors (catecholamine, serotonine, histamine, acetylcholine) ; Nicotinic Antagonists ; Nicotinic receptor ; Trimethaphan - pharmacology ; κ-bungarotoxin</subject><ispartof>Brain research, 1992-02, Vol.573 (1), p.77-82</ispartof><rights>1992 Elsevier Science Publishers B.V. All rights reserved</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-79ac0a9aec882b531782ed217f7bcff48a60c0bab4ef858a0bdaf39d7105f9ea3</citedby><cites>FETCH-LOGICAL-c417t-79ac0a9aec882b531782ed217f7bcff48a60c0bab4ef858a0bdaf39d7105f9ea3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/000689939290115P$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5110077$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1576536$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nooney, Janet M.</creatorcontrib><creatorcontrib>Lambert, Jeremy J.</creatorcontrib><creatorcontrib>Chiappinelli, Vincent A.</creatorcontrib><title>The interaction of κ-bungarotoxin with the nicotinic receptor of bovine chromaffin cells</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>Whole-cell recording techniques were used to examine acetylcholine-induced nicotinic currents in isolated bovinei chromaffin cells. The effects on these currents of κ-bungarotoxin, a snake venon κ-neurotoxin, were tested. Exposure of cells to κ-bungarotoxin (600 nM for 40 min) produced a prolonged blockade of nicotinic currents. The mechanism of this blockade was examined in several ways. Firstly, the pre-exposure of cells to trimetaphan, a competitive nicotinic antagonist, protected against the action of subsequent additions of κ-bungarotoxin. Secondly, voltage-clamp measurements indicated that the degree of blockade produced by κ-bungarotoxin was independent of cell membrane potential. Unlike (+)-tubocurarine, κ-bungarotoxin had no direct agonist effects on nicotinic receptors. It is concluded from the present functional studies and from previously reported binding studies that κ-bungarotoxin blocks nicotinic responses in bovine chromaffin cells by binding to regions overlying acetylcholine sites on nicotinic receptors.</description><subject>Acetylcholine</subject><subject>Acetylcholine - pharmacology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bovine adrenomedullary chromaffin cell</subject><subject>Bungarotoxins - pharmacology</subject><subject>Cattle</subject><subject>Cell receptors</subject><subject>Cell structures and functions</subject><subject>Cells, Cultured</subject><subject>Enterochromaffin Cells - drug effects</subject><subject>Enterochromaffin Cells - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Ganglionic Stimulants - pharmacology</subject><subject>Molecular and cellular biology</subject><subject>Monoamines receptors (catecholamine, serotonine, histamine, acetylcholine)</subject><subject>Nicotinic Antagonists</subject><subject>Nicotinic receptor</subject><subject>Trimethaphan - pharmacology</subject><subject>κ-bungarotoxin</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEuOEzEQhi00KGQCNwCpFyM0LBpc_XJ7MxKKeEkjkUVYsLKq3WVilNgZ2xngahyCM-EmUWbHpkql-v5S6WPsOfDXwKF7wznvyl7K-lpWryQHaMvVIzaHXlRlVzX8gs3PyBN2GeP3PNa15DM2g1Z0bd3N2df1hgrrEgXUyXpXeFP8-V0OB_cNg0_-p3XFD5s2Rcqcs9onm2sRSNM--TDhg7-3jgq9CX6HxuSApu02PmWPDW4jPTv1Bfvy_t16-bG8_fzh0_LtbakbEKkUEjVHiaT7vhraGkRf0ViBMGLQxjQ9dlzzAYeGTN_2yIcRTS1HAbw1krBesJfHu_vg7w4Uk9rZOH2AjvwhKuggG2ghg80R1MHHGMiofbA7DL8UcDUZVZMuNelSslL_jKpVjr043T8MOxofQkeFeX912mPUuDUBnbbxjLUAnAuRsZsjRtnFvaWgorbkNI02y0xq9Pb_f_wFTkyUjQ</recordid><startdate>19920221</startdate><enddate>19920221</enddate><creator>Nooney, Janet M.</creator><creator>Lambert, Jeremy J.</creator><creator>Chiappinelli, Vincent A.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>19920221</creationdate><title>The interaction of κ-bungarotoxin with the nicotinic receptor of bovine chromaffin cells</title><author>Nooney, Janet M. ; Lambert, Jeremy J. ; Chiappinelli, Vincent A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-79ac0a9aec882b531782ed217f7bcff48a60c0bab4ef858a0bdaf39d7105f9ea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Acetylcholine</topic><topic>Acetylcholine - pharmacology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Bovine adrenomedullary chromaffin cell</topic><topic>Bungarotoxins - pharmacology</topic><topic>Cattle</topic><topic>Cell receptors</topic><topic>Cell structures and functions</topic><topic>Cells, Cultured</topic><topic>Enterochromaffin Cells - drug effects</topic><topic>Enterochromaffin Cells - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Ganglionic Stimulants - pharmacology</topic><topic>Molecular and cellular biology</topic><topic>Monoamines receptors (catecholamine, serotonine, histamine, acetylcholine)</topic><topic>Nicotinic Antagonists</topic><topic>Nicotinic receptor</topic><topic>Trimethaphan - pharmacology</topic><topic>κ-bungarotoxin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nooney, Janet M.</creatorcontrib><creatorcontrib>Lambert, Jeremy J.</creatorcontrib><creatorcontrib>Chiappinelli, Vincent A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nooney, Janet M.</au><au>Lambert, Jeremy J.</au><au>Chiappinelli, Vincent A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The interaction of κ-bungarotoxin with the nicotinic receptor of bovine chromaffin cells</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>1992-02-21</date><risdate>1992</risdate><volume>573</volume><issue>1</issue><spage>77</spage><epage>82</epage><pages>77-82</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>Whole-cell recording techniques were used to examine acetylcholine-induced nicotinic currents in isolated bovinei chromaffin cells. The effects on these currents of κ-bungarotoxin, a snake venon κ-neurotoxin, were tested. Exposure of cells to κ-bungarotoxin (600 nM for 40 min) produced a prolonged blockade of nicotinic currents. The mechanism of this blockade was examined in several ways. Firstly, the pre-exposure of cells to trimetaphan, a competitive nicotinic antagonist, protected against the action of subsequent additions of κ-bungarotoxin. Secondly, voltage-clamp measurements indicated that the degree of blockade produced by κ-bungarotoxin was independent of cell membrane potential. Unlike (+)-tubocurarine, κ-bungarotoxin had no direct agonist effects on nicotinic receptors. It is concluded from the present functional studies and from previously reported binding studies that κ-bungarotoxin blocks nicotinic responses in bovine chromaffin cells by binding to regions overlying acetylcholine sites on nicotinic receptors.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>1576536</pmid><doi>10.1016/0006-8993(92)90115-P</doi><tpages>6</tpages></addata></record> |
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| ispartof | Brain research, 1992-02, Vol.573 (1), p.77-82 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Acetylcholine Acetylcholine - pharmacology Animals Biological and medical sciences Bovine adrenomedullary chromaffin cell Bungarotoxins - pharmacology Cattle Cell receptors Cell structures and functions Cells, Cultured Enterochromaffin Cells - drug effects Enterochromaffin Cells - metabolism Fundamental and applied biological sciences. Psychology Ganglionic Stimulants - pharmacology Molecular and cellular biology Monoamines receptors (catecholamine, serotonine, histamine, acetylcholine) Nicotinic Antagonists Nicotinic receptor Trimethaphan - pharmacology κ-bungarotoxin |
| title | The interaction of κ-bungarotoxin with the nicotinic receptor of bovine chromaffin cells |
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