Visual Detection of Norovirus Genogroup II by Reverse Transcription Loop-Mediated Isothermal Amplification with Hydroxynaphthol Blue Dye
A simple, rapid, specific, and sensitive colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay with hydroxynaphthol blue dye (HNB) was established, targeting RNA-dependent RNA polymerase and capsid protein gene for the detection of the dominant norovirus genogroup...
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| Veröffentlicht in: | Food and environmental virology 2014-09, Vol.6 (3), p.196-201 |
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| creator | Luo, Jianming Xu, Ziqian Nie, Kai Ding, Xiong Guan, Li Wang, Ji Xian, Yuying Wu, Xiyang Ma, Xuejun |
| description | A simple, rapid, specific, and sensitive colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay with hydroxynaphthol blue dye (HNB) was established, targeting RNA-dependent RNA polymerase and capsid protein gene for the detection of the dominant norovirus genogroup in China—NoV GII. The assay was carried out at 65 °C for 60 min with no cross-reactivity with other common gastroenteritis viruses. The sensitivity of this assay was 10
3
copies per reaction which is equivalent to the conventional RT-PCR test. The clinical test showed 94.83 % coincidence rate for NoV genogroup II detection compared with the results, confirmed by the Department of Viral Diarrhea of Chinese Center for Disease Control and Prevention via conventional RT-PCR. The HNB dye-based RT-LAMP could be a novel rapid screening method for prevalent norovirus genogroup II in China, especially in those resource-limited hospitals and rural local clinics. |
| doi_str_mv | 10.1007/s12560-014-9142-8 |
| format | Article |
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3
copies per reaction which is equivalent to the conventional RT-PCR test. The clinical test showed 94.83 % coincidence rate for NoV genogroup II detection compared with the results, confirmed by the Department of Viral Diarrhea of Chinese Center for Disease Control and Prevention via conventional RT-PCR. The HNB dye-based RT-LAMP could be a novel rapid screening method for prevalent norovirus genogroup II in China, especially in those resource-limited hospitals and rural local clinics.</description><identifier>ISSN: 1867-0334</identifier><identifier>EISSN: 1867-0342</identifier><identifier>DOI: 10.1007/s12560-014-9142-8</identifier><identifier>PMID: 24752892</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Assaying ; Biomedical and Life Sciences ; Biomedicine ; Caliciviridae Infections - virology ; Capsid protein ; Chemistry/Food Science ; China ; Colorimetry ; Coloring Agents - chemistry ; Coloring Agents - metabolism ; Cross-reactivity ; Diarrhea ; Disease control ; DNA-directed RNA polymerase ; Dyes ; Food Science ; Gastroenteritis ; Gene amplification ; Genotype ; Humans ; Naphthalenesulfonates - chemistry ; Naphthalenesulfonates - metabolism ; Norovirus - chemistry ; Norovirus - genetics ; Norovirus - isolation & purification ; Nucleic Acid Amplification Techniques - instrumentation ; Nucleic Acid Amplification Techniques - methods ; Original Paper ; Polymerase chain reaction ; Reverse Transcription ; Ribonucleic acid ; RNA ; RNA polymerase ; RNA-directed RNA polymerase ; Viral Proteins - genetics ; Virology ; Viruses</subject><ispartof>Food and environmental virology, 2014-09, Vol.6 (3), p.196-201</ispartof><rights>Springer Science+Business Media New York 2014</rights><rights>Springer Science+Business Media New York 2014.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c485t-a04653b089eeec81ad4afbdaf531f0d09ae3a3eb2b074256aa8dc55af5d71dfa3</citedby><cites>FETCH-LOGICAL-c485t-a04653b089eeec81ad4afbdaf531f0d09ae3a3eb2b074256aa8dc55af5d71dfa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12560-014-9142-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12560-014-9142-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24752892$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Luo, Jianming</creatorcontrib><creatorcontrib>Xu, Ziqian</creatorcontrib><creatorcontrib>Nie, Kai</creatorcontrib><creatorcontrib>Ding, Xiong</creatorcontrib><creatorcontrib>Guan, Li</creatorcontrib><creatorcontrib>Wang, Ji</creatorcontrib><creatorcontrib>Xian, Yuying</creatorcontrib><creatorcontrib>Wu, Xiyang</creatorcontrib><creatorcontrib>Ma, Xuejun</creatorcontrib><title>Visual Detection of Norovirus Genogroup II by Reverse Transcription Loop-Mediated Isothermal Amplification with Hydroxynaphthol Blue Dye</title><title>Food and environmental virology</title><addtitle>Food Environ Virol</addtitle><addtitle>Food Environ Virol</addtitle><description>A simple, rapid, specific, and sensitive colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay with hydroxynaphthol blue dye (HNB) was established, targeting RNA-dependent RNA polymerase and capsid protein gene for the detection of the dominant norovirus genogroup in China—NoV GII. The assay was carried out at 65 °C for 60 min with no cross-reactivity with other common gastroenteritis viruses. The sensitivity of this assay was 10
3
copies per reaction which is equivalent to the conventional RT-PCR test. The clinical test showed 94.83 % coincidence rate for NoV genogroup II detection compared with the results, confirmed by the Department of Viral Diarrhea of Chinese Center for Disease Control and Prevention via conventional RT-PCR. The HNB dye-based RT-LAMP could be a novel rapid screening method for prevalent norovirus genogroup II in China, especially in those resource-limited hospitals and rural local clinics.</description><subject>Assaying</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Caliciviridae Infections - virology</subject><subject>Capsid protein</subject><subject>Chemistry/Food Science</subject><subject>China</subject><subject>Colorimetry</subject><subject>Coloring Agents - chemistry</subject><subject>Coloring Agents - metabolism</subject><subject>Cross-reactivity</subject><subject>Diarrhea</subject><subject>Disease control</subject><subject>DNA-directed RNA polymerase</subject><subject>Dyes</subject><subject>Food Science</subject><subject>Gastroenteritis</subject><subject>Gene amplification</subject><subject>Genotype</subject><subject>Humans</subject><subject>Naphthalenesulfonates - chemistry</subject><subject>Naphthalenesulfonates - metabolism</subject><subject>Norovirus - chemistry</subject><subject>Norovirus - genetics</subject><subject>Norovirus - isolation & purification</subject><subject>Nucleic Acid Amplification Techniques - instrumentation</subject><subject>Nucleic Acid Amplification Techniques - methods</subject><subject>Original Paper</subject><subject>Polymerase chain reaction</subject><subject>Reverse Transcription</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA polymerase</subject><subject>RNA-directed RNA polymerase</subject><subject>Viral Proteins - genetics</subject><subject>Virology</subject><subject>Viruses</subject><issn>1867-0334</issn><issn>1867-0342</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kV9L3TAYh8OYTOf2AbyRwG686cy_tumlU6cHjhsMt9vwtnnribRNl7S6foN97EWPOhgMAgm8z--XkIeQA84-csbK48hFXrCMcZVVXIlMvyJ7XBdlxqQSr1_OUu2StzHeMlZIkcs3ZFeoMhe6Envk9w8XZ-joGU7YTM4P1Lf0iw_-zoU50gsc_E3w80hXK1ov9BveYYhIrwMMsQlufIysvR-zK7QOJrR0Ff20wdCn1pN-7FzrGnjE7t20oZeLDf7XMsC4mTa-o5-6GenZgu_ITgtdxPdP-z75_vn8-vQyW3-9WJ2erLNG6XzKgKkilzXTFSI2moNV0NYW2lzylllWAUqQWIualSr9DoC2TZ6nuS25bUHuk6Nt7xj8zxnjZHoXG-w6GNDP0fCCay3Skgn98A966-cwpNcZoZiSVVHIIlF8SzXBxxiwNWNwPYTFcGYeNJmtJpM0mQdNRqfM4VPzXPdoXxLPXhIgtkBMo-EGw9-r_9_6B8teoEs</recordid><startdate>20140901</startdate><enddate>20140901</enddate><creator>Luo, Jianming</creator><creator>Xu, Ziqian</creator><creator>Nie, Kai</creator><creator>Ding, Xiong</creator><creator>Guan, Li</creator><creator>Wang, Ji</creator><creator>Xian, Yuying</creator><creator>Wu, Xiyang</creator><creator>Ma, Xuejun</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140901</creationdate><title>Visual Detection of Norovirus Genogroup II by Reverse Transcription Loop-Mediated Isothermal Amplification with Hydroxynaphthol Blue Dye</title><author>Luo, Jianming ; Xu, Ziqian ; Nie, Kai ; Ding, Xiong ; Guan, Li ; Wang, Ji ; Xian, Yuying ; Wu, Xiyang ; Ma, Xuejun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c485t-a04653b089eeec81ad4afbdaf531f0d09ae3a3eb2b074256aa8dc55af5d71dfa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Assaying</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Caliciviridae Infections - virology</topic><topic>Capsid protein</topic><topic>Chemistry/Food Science</topic><topic>China</topic><topic>Colorimetry</topic><topic>Coloring Agents - chemistry</topic><topic>Coloring Agents - metabolism</topic><topic>Cross-reactivity</topic><topic>Diarrhea</topic><topic>Disease control</topic><topic>DNA-directed RNA polymerase</topic><topic>Dyes</topic><topic>Food Science</topic><topic>Gastroenteritis</topic><topic>Gene amplification</topic><topic>Genotype</topic><topic>Humans</topic><topic>Naphthalenesulfonates - chemistry</topic><topic>Naphthalenesulfonates - metabolism</topic><topic>Norovirus - chemistry</topic><topic>Norovirus - genetics</topic><topic>Norovirus - isolation & purification</topic><topic>Nucleic Acid Amplification Techniques - instrumentation</topic><topic>Nucleic Acid Amplification Techniques - methods</topic><topic>Original Paper</topic><topic>Polymerase chain reaction</topic><topic>Reverse Transcription</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA polymerase</topic><topic>RNA-directed RNA polymerase</topic><topic>Viral Proteins - genetics</topic><topic>Virology</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Luo, Jianming</creatorcontrib><creatorcontrib>Xu, Ziqian</creatorcontrib><creatorcontrib>Nie, Kai</creatorcontrib><creatorcontrib>Ding, Xiong</creatorcontrib><creatorcontrib>Guan, Li</creatorcontrib><creatorcontrib>Wang, Ji</creatorcontrib><creatorcontrib>Xian, Yuying</creatorcontrib><creatorcontrib>Wu, Xiyang</creatorcontrib><creatorcontrib>Ma, Xuejun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Food and environmental virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Luo, Jianming</au><au>Xu, Ziqian</au><au>Nie, Kai</au><au>Ding, Xiong</au><au>Guan, Li</au><au>Wang, Ji</au><au>Xian, Yuying</au><au>Wu, Xiyang</au><au>Ma, Xuejun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Visual Detection of Norovirus Genogroup II by Reverse Transcription Loop-Mediated Isothermal Amplification with Hydroxynaphthol Blue Dye</atitle><jtitle>Food and environmental virology</jtitle><stitle>Food Environ Virol</stitle><addtitle>Food Environ Virol</addtitle><date>2014-09-01</date><risdate>2014</risdate><volume>6</volume><issue>3</issue><spage>196</spage><epage>201</epage><pages>196-201</pages><issn>1867-0334</issn><eissn>1867-0342</eissn><abstract>A simple, rapid, specific, and sensitive colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay with hydroxynaphthol blue dye (HNB) was established, targeting RNA-dependent RNA polymerase and capsid protein gene for the detection of the dominant norovirus genogroup in China—NoV GII. The assay was carried out at 65 °C for 60 min with no cross-reactivity with other common gastroenteritis viruses. The sensitivity of this assay was 10
3
copies per reaction which is equivalent to the conventional RT-PCR test. The clinical test showed 94.83 % coincidence rate for NoV genogroup II detection compared with the results, confirmed by the Department of Viral Diarrhea of Chinese Center for Disease Control and Prevention via conventional RT-PCR. The HNB dye-based RT-LAMP could be a novel rapid screening method for prevalent norovirus genogroup II in China, especially in those resource-limited hospitals and rural local clinics.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>24752892</pmid><doi>10.1007/s12560-014-9142-8</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Assaying Biomedical and Life Sciences Biomedicine Caliciviridae Infections - virology Capsid protein Chemistry/Food Science China Colorimetry Coloring Agents - chemistry Coloring Agents - metabolism Cross-reactivity Diarrhea Disease control DNA-directed RNA polymerase Dyes Food Science Gastroenteritis Gene amplification Genotype Humans Naphthalenesulfonates - chemistry Naphthalenesulfonates - metabolism Norovirus - chemistry Norovirus - genetics Norovirus - isolation & purification Nucleic Acid Amplification Techniques - instrumentation Nucleic Acid Amplification Techniques - methods Original Paper Polymerase chain reaction Reverse Transcription Ribonucleic acid RNA RNA polymerase RNA-directed RNA polymerase Viral Proteins - genetics Virology Viruses |
| title | Visual Detection of Norovirus Genogroup II by Reverse Transcription Loop-Mediated Isothermal Amplification with Hydroxynaphthol Blue Dye |
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