Oral administration of whole dihomo-γ-linolenic acid-producing Saccharomyces cerevisiae suppresses cutaneous inflammatory responses induced by croton oil application in mice

Polyunsaturated fatty acids have been attracting considerable interest because of their many biological activities and important roles in human health and nutrition. Dihomo-γ-linolenic acid (DGLA; C20: 3n-6) is known to have an anti-inflammatory activity, but its range of effects was not well studie...

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Veröffentlicht in:Applied microbiology and biotechnology 2014-10, Vol.98 (20), p.8697-8706
Hauptverfasser: Watanabe, Naoko, Masubuchi, Daiki, Itoh, Maki, Teradu, Soichiro, Yazawa, Hisashi, Uemura, Hiroshi
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container_issue 20
container_start_page 8697
container_title Applied microbiology and biotechnology
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creator Watanabe, Naoko
Masubuchi, Daiki
Itoh, Maki
Teradu, Soichiro
Yazawa, Hisashi
Uemura, Hiroshi
description Polyunsaturated fatty acids have been attracting considerable interest because of their many biological activities and important roles in human health and nutrition. Dihomo-γ-linolenic acid (DGLA; C20: 3n-6) is known to have an anti-inflammatory activity, but its range of effects was not well studied because of its limited natural sources. Taking advantage of genetic tractability and increasing wealth of accessible data of Saccharomyces cerevisiae, we have previously constructed a DGLA-producing yeast strain by introducing two types of desaturase and one elongase genes to convert endogenous oleic acid (C18:1n-9) to DGLA. In this study, we investigated the efficacy of oral intake of heat-killed whole DGLA-producing yeast cells in the absence of lipid purification on cutaneous inflammation. Topical application of croton oil to mouse ears induces ear swelling in parallel with the increased production of chemokines and accumulation of infiltrating cells into the skin sites. These inflammatory reactions were significantly suppressed in a dose-dependent manner by oral intake of the DGLA-producing yeast cells for only 7 days. This suppression was not observed by the intake of the γ-linolenic acid-producing (C18:3n-6, an immediate precursor of DGLA) yeast, indicating DGLA itself suppressed the inflammation. Further analysis demonstrated that DGLA exerted an anti-inflammatory effect via prostaglandin E1 formation because naproxen, a cyclooxygenase inhibitor, attenuated the suppression. Since 25-fold of purified DGLA compared with that provided as a form of yeast was not effective, oral administration of the whole DGLA-producing yeast is considered to be a simple but efficient method to suppress inflammatory responses.
doi_str_mv 10.1007/s00253-014-5949-3
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This suppression was not observed by the intake of the γ-linolenic acid-producing (C18:3n-6, an immediate precursor of DGLA) yeast, indicating DGLA itself suppressed the inflammation. Further analysis demonstrated that DGLA exerted an anti-inflammatory effect via prostaglandin E1 formation because naproxen, a cyclooxygenase inhibitor, attenuated the suppression. 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subjects 8,11,14-Eicosatrienoic Acid - metabolism
Administration, Oral
Analysis
Animals
anti-inflammatory activity
Anti-Inflammatory Agents - metabolism
Applied Microbial and Cell Physiology
Biological Therapy - methods
Biomedical and Life Sciences
Biotechnology
Brewer's yeast
chemokines
Croton
Croton Oil - toxicity
Dermatitis - prevention & control
Disease Models, Animal
ears
genes
human health
Inflammation
Life Sciences
Metabolic Engineering
Mice
Microbial Genetics and Genomics
Microbiology
nutrition
oils
oleic acid
Oleic Acid - metabolism
oral administration
Physiological aspects
polyunsaturated fatty acids
prostaglandin synthase
prostaglandins
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
topical application
yeasts
title Oral administration of whole dihomo-γ-linolenic acid-producing Saccharomyces cerevisiae suppresses cutaneous inflammatory responses induced by croton oil application in mice
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